Restriction map for pF1KA1018
 (Linear) (Six-base) MAPSORT of: KIAA1018.seq  Check: 6902  from: 1  to: 3051



KIAA1018.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     123    3051 
   Size:        123    2928
 
AccI GT'mk_AC

Cuts at:      0     829    3051 
   Size:        829    2222
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     572     818     911    1418    2496    2559    2758    3017 
   Size:        572     246      93     507    1078      63     199     259

Cuts at:   3017    3051 
   Size:         34

  Fragments arranged by size: 

                1078     572     507     259     246     199      93      63
                  34
 
AflIII A'CryG_T

Cuts at:      0     641    2042    2334    3051 
   Size:        641    1401     292     717

  Fragments arranged by size: 

                1401     717     641     292
 
AgeI A'CCGG_T

Cuts at:      0    1108    3051 
   Size:       1108    1943
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2858    3051 
   Size:       2858     193
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1237    1378    1485    2988    3051 
   Size:       1237     141     107    1503      63

  Fragments arranged by size: 

                1503    1237     141     107      63
 
ApaI G_GGCC'C

Cuts at:      0    2812    3051 
   Size:       2812     239
 
ApaLI G'TGCA_C

Cuts at:      0    2610    3051 
   Size:       2610     441
 
ApoI r'AATT_y

Cuts at:      0     240     321     523     611    1216    2090    3051 
   Size:        240      81     202      88     605     874     961

  Fragments arranged by size: 

                 961     874     605     240     202      88      81
 
AvaI C'yCGr_G

Cuts at:      0     734    2680    3051 
   Size:        734    1946     371

  Fragments arranged by size: 

                1946     734     371
 
BamHI G'GATC_C

Cuts at:      0     216    2202    3051 
   Size:        216    1986     849

  Fragments arranged by size: 

                1986     849     216
 
BanI G'GyrC_C

Cuts at:      0     150     173    2834    3051 
   Size:        150      23    2661     217

  Fragments arranged by size: 

                2661     217     150      23
 
BanII G_rGCy'C

Cuts at:      0    1889    2812    3051 
   Size:       1889     923     239

  Fragments arranged by size: 

                1889     923     239
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     845    1688    2565    3051 
   Size:        845     843     877     486

  Fragments arranged by size: 

                 877     845     843     486
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2407    2590    2650    2704    2807    3051 
   Size:       2407     183      60      54     103     244

  Fragments arranged by size: 

                2407     244     183     103      60      54
 
Bme1580I G_kGCm'C

Cuts at:      0    2362    2614    2812    3051 
   Size:       2362     252     198     239

  Fragments arranged by size: 

                2362     252     239     198
 
BmgBI CAC'GTC

Cuts at:      0    2768    3051 
   Size:       2768     283
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1878    3051 
   Size:       1878    1173
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2887    3051 
   Size:       2887     164
 
Bpu10I CC'TnA_GC

Cuts at:      0    1880    2783    3046    3051 
   Size:       1880     903     263       5

  Fragments arranged by size: 

                1880     903     263       5
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1180    2668    3051 
   Size:       1180    1488     383

  Fragments arranged by size: 

                1488    1180     383
 
BsaAI yAC'GTr

Cuts at:      0     489    2337    2714    3051 
   Size:        489    1848     377     337

  Fragments arranged by size: 

                1848     489     377     337
 
BsaHI Gr'CG_yC

Cuts at:      0    1493    1685    2637    3051 
   Size:       1493     192     952     414

  Fragments arranged by size: 

                1493     952     414     192
 
BsaWI w'CCGG_w

Cuts at:      0    1108    2205    2565    3051 
   Size:       1108    1097     360     486

  Fragments arranged by size: 

                1108    1097     486     360
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1063    1093    3051 
   Size:       1063      30    1958

  Fragments arranged by size: 

                1958    1063      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1007    2458    2521    2698    3051 
   Size:       1007    1451      63     177     353

  Fragments arranged by size: 

                1451    1007     353     177      63
 
BseYI C'CCAG_C

Cuts at:      0    2640    2753    2988    3051 
   Size:       2640     113     235      63

  Fragments arranged by size: 

                2640     235     113      63
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1512    1580    2850    3051 
   Size:       1512      68    1270     201

  Fragments arranged by size: 

                1512    1270     201      68
 
BsiEI CG_ry'CG

Cuts at:      0    1740    3051 
   Size:       1740    1311
 
BsiHKAI G_wGCw'C

Cuts at:      0    1889    2437    2614    2819    3051 
   Size:       1889     548     177     205     232

  Fragments arranged by size: 

                1889     548     232     205     177
 
BsmI GAATG_Cn'

Cuts at:      0     674    1061    1359    2593    3051 
   Size:        674     387     298    1234     458

  Fragments arranged by size: 

                1234     674     458     387     298
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2774    3051 
   Size:       2774     277
 
Bsp1286I G_dGCh'C

Cuts at:      0    1889    2362    2437    2614    2812    2819    3051 
   Size:       1889     473      75     177     198       7     232

  Fragments arranged by size: 

                1889     473     232     198     177      75       7
 
BspEI T'CCGG_A

Cuts at:      0    2205    2565    3051 
   Size:       2205     360     486

  Fragments arranged by size: 

                2205     486     360
 
BspHI T'CATG_A

Cuts at:      0    2670    3051 
   Size:       2670     381
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     123     216    3051 
   Size:        123      93    2835

  Fragments arranged by size: 

                2835     123      93
 
BsrDI GCAATG_nn'

Cuts at:      0    1095    1859    3051 
   Size:       1095     764    1192

  Fragments arranged by size: 

                1192    1095     764
 
BsrFI r'CCGG_y

Cuts at:      0     170     529    1108    3051 
   Size:        170     359     579    1943

  Fragments arranged by size: 

                1943     579     359     170
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     115     985    2357    3051 
   Size:        115     870    1372     694

  Fragments arranged by size: 

                1372     870     694     115
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1587    3051 
   Size:       1587    1464
 
BstYI r'GATC_y

Cuts at:      0     216    2202    2787    3051 
   Size:        216    1986     585     264

  Fragments arranged by size: 

                1986     585     264     216
 
BtgI C'CryG_G

Cuts at:      0    2423    3051 
   Size:       2423     628
 
BtsI GCAGTG_nn'

Cuts at:      0    1568    3051 
   Size:       1568    1483
 
DraI TTT'AAA

Cuts at:      0     387     648    1571    3051 
   Size:        387     261     923    1480

  Fragments arranged by size: 

                1480     923     387     261
 
DraIII CAC_nnn'GTG

Cuts at:      0    2368    3051 
   Size:       2368     683
 
EaeI y'GGCC_r

Cuts at:      0     333    1508    1737    1856    3051 
   Size:        333    1175     229     119    1195

  Fragments arranged by size: 

                1195    1175     333     229     119
 
EagI C'GGCC_G

Cuts at:      0    1737    3051 
   Size:       1737    1314
 
EarI CTCTTCn'nnn_

Cuts at:      0     591     662    1304    1969    2299    2777    3051 
   Size:        591      71     642     665     330     478     274

  Fragments arranged by size: 

                 665     642     591     478     330     274      71
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2215    3051 
   Size:       2215     836
 
EcoICRI GAG'CTC

Cuts at:      0    1887    3051 
   Size:       1887    1164
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     572     818     911    1418    2496    2559    2758    3017 
   Size:        572     246      93     507    1078      63     199     259

Cuts at:   3017    3051 
   Size:         34

  Fragments arranged by size: 

                1078     572     507     259     246     199      93      63
                  34
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1618    2808    2809    2868    2932    3051 
   Size:       1618    1190       1      59      64     119

  Fragments arranged by size: 

                1618    1190     119      64      59       1
 
EcoRV GAT'ATC

Cuts at:      0    1050    3051 
   Size:       1050    2001
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     862    2310    3051 
   Size:        862    1448     741

  Fragments arranged by size: 

                1448     862     741
 
HaeII r_GCGC'y

Cuts at:      0    2163    3051 
   Size:       2163     888
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     949     981    1063    1095    1995    2027    2779    2811 
   Size:        949      32      82      32     900      32     752      32

Cuts at:   2811    3051 
   Size:        240

  Fragments arranged by size: 

                 949     900     752     240      82      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1661    1731    3051 
   Size:       1661      70    1320

  Fragments arranged by size: 

                1661    1320      70
 
HindIII A'AGCT_T

Cuts at:      0      45    3051 
   Size:         45    3006
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    3006    3051 
   Size:       3006      45
 
MscI TGG'CCA

Cuts at:      0     335    1510    1858    3051 
   Size:        335    1175     348    1193

  Fragments arranged by size: 

                1193    1175     348     335
 
MslI CAynn'nnrTG

Cuts at:      0     179    1828    2045    2551    3051 
   Size:        179    1649     217     506     500

  Fragments arranged by size: 

                1649     506     500     217     179
 
MspA1I CmG'CkG

Cuts at:      0     945    1485    2413    2474    2664    2753    3051 
   Size:        945     540     928      61     190      89     298

  Fragments arranged by size: 

                 945     928     540     298     190      89      61
 
NdeI CA'TA_TG

Cuts at:      0     686    3051 
   Size:        686    2365
 
NspI r_CATG'y

Cuts at:      0     896    1833    2046    3051 
   Size:        896     937     213    1005

  Fragments arranged by size: 

                1005     937     896     213
 
PasI CC'CwG_GG

Cuts at:      0    1622    3051 
   Size:       1622    1429
 
PciI A'CATG_T

Cuts at:      0    2042    3051 
   Size:       2042    1009
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2368    3051 
   Size:       2368     683
 
PmlI CAC'GTG

Cuts at:      0    2337    2714    3051 
   Size:       2337     377     337

  Fragments arranged by size: 

                2337     377     337
 
PspOMI G'GGCC_C

Cuts at:      0    2808    3051 
   Size:       2808     243
 
PstI C_TGCA'G

Cuts at:      0     981    1025    2033    2663    2984    3051 
   Size:        981      44    1008     630     321      67

  Fragments arranged by size: 

                1008     981     630     321      67      44
 
PvuII CAG'CTG

Cuts at:      0     945    1485    2664    2753    3051 
   Size:        945     540    1179      89     298

  Fragments arranged by size: 

                1179     945     540     298      89
 
SacI G_AGCT'C

Cuts at:      0    1889    3051 
   Size:       1889    1162
 
SexAI A'CCwGG_T

Cuts at:      0    2879    3051 
   Size:       2879     172
 
SfcI C'TryA_G

Cuts at:      0     977    1021    1366    2029    2659    2980    3051 
   Size:        977      44     345     663     630     321      71

  Fragments arranged by size: 

                 977     663     630     345     321      71      44
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2407    3051 
   Size:       2407     644
 
SmaI CCC'GGG

Cuts at:      0     736    3051 
   Size:        736    2315
 
SmlI C'TyrA_G

Cuts at:      0    1159    2647    3051 
   Size:       1159    1488     404

  Fragments arranged by size: 

                1488    1159     404
 
SnaBI TAC'GTA

Cuts at:      0     489    3051 
   Size:        489    2562
 
StuI AGG'CCT

Cuts at:      0      36    3051 
   Size:         36    3015
 
StyI C'CwwG_G

Cuts at:      0    1586    3051 
   Size:       1586    1465
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1678    1976    2691    3051 
   Size:       1678     298     715     360

  Fragments arranged by size: 

                1678     715     360     298
 
Tth111I GACn'n_nGTC

Cuts at:      0    2858    3051 
   Size:       2858     193
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2491    3051 
   Size:       2491     560
 
XmaI C'CCGG_G

Cuts at:      0     734    3051 
   Size:        734    2317
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AgeI     AlfI    AlwNI     ApaI
    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbsI     BglI
 Bme1580I    BmgBI     BmrI     BplI   Bpu10I    BpuEI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrFI   BstAPI
    BstXI    BstYI     BtgI     BtsI     DraI   DraIII     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRV     FalI    HaeII
    Hin4I   HincII  HindIII     MmeI     MscI     MslI   MspA1I     NdeI
     NspI     PasI     PciI    PflMI     PmlI   PspOMI     PstI    PvuII
     SacI    SexAI     SfcI     SfiI     SmaI     SmlI    SnaBI     StuI
     StyI    TaqII  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AhdI     AleI     AloI
     AscI     AseI    AsiSI    AvrII     BaeI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI     BmtI     BpmI     BsaI
    BsaBI    BsiWI    BsrBI    BsrGI   BssHII    BssSI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DrdI    EcoNI    EcoRI
     FseI     FspI    FspAI     HpaI     KasI     KpnI     MfeI     MluI
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SfoI     SgfI    SgrAI     SpeI     SphI     SrfI     SspI
     SwaI     TatI     TstI     XbaI     XhoI     XmnI     ZraI
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