Restriction map for pF1KA0987
##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

AatII       Acc65I      AccI        AflII       AgeI        
AocI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp700I     Asp718I     AviII       BamHI       
BbrPI       BfrI        BglI        BsiWI       Bsp1407I    
BspDI       BspHI       BsrDI       BsrGI       Bst1107I    
BstEII      BstPI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DraIII      DsaI        Eco47III    
Eco81I      EcoO65I     FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MfeI        MluI        MunI        NaeI        NcoI        
NgoMIV      NheI        NotI        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshBI       PvuI        PvuII       RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SfiI        
SgrAI       SmaI        SpeI        Sse8387I    SspBI       
SspI        SwaI        XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AclI         1307  AACGTT               AA^CGTT
AflIII       3197  ACATGT               A^C[GA][CT]GT
AhdI          634  GACATCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         634  GACATCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2487  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         2043  CCCGAG               C^[CT]CG[GA]G
AvrII        1695  CCTAGG               C^CTAGG
BalI         1350  TGGCCA               TGG^CCA
BanI           90  GGCGCC               G^G[CT][GA]CC
BbeI           90  GGCGCC               GGCGC^C
BbvCI        1420  GCTGAGG              CCTCAGC(-5/-2)
BcgI         2915  CGAAGGAAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1810  GGATAC               GTATCC(6/5)
BclI         1391  TGATCA               T^GATCA
BglII        2988  AGATCT               A^GATCT
BlnI         1695  CCTAGG               C^CTAGG
BsaAI         459  TACGTA               [CT]AC^GT[GA]
BsaBI         895  GATTTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        2506  GAGACG               CGTCTC(1/5)
BsmI         2653  GCATTC               GAATGC(1/-1)
BsoBI        2043  CCCGAG               C^[CT]CG[GA]G
BspLU11I     3197  ACATGT               A^CATGT
BsrFI         974  ACCGGC               [GA]^CCGG[CT]
BssHII         81  GCGCGC               G^CGCGC
BssSI        1495  CACGAG               CACGAG(-5/-1)
BstBI         524  TTCGAA               TT^CGAA
BstXI        3209  CCAAAGTAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        974  ACCGGC               [GA]^CCGG[CT]
DraII         128  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DrdI         2349  GACTAAGCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1054  CGGCCG               C^GGCCG
Eam1105I      634  GACATCGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1054  CGGCCG               C^GGCCG
Eco52I       1054  CGGCCG               C^GGCCG
EcoO109I      128  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRI        2428  GAATTC               G^AATTC
EcoRV         595  GATATC               GAT^ATC
EcoT22I       850  ATGCAT               ATGCA^T
FbaI         1391  TGATCA               T^GATCA
HgaI          831  GACGC                GACGC(5/10)
KasI           90  GGCGCC               G^GCGCC
MamI          895  GATTTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1350  TGGCCA               TGG^CCA
MscI         1350  TGGCCA               TGG^CCA
NarI           90  GGCGCC               GG^CGCC
NdeI         2957  CATATG               CA^TATG
NruI          969  TCGCGA               TCG^CGA
NsiI          850  ATGCAT               ATGCA^T
NspV          524  TTCGAA               TT^CGAA
PflFI        2487  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        1525  GACTCATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     1307  AACGTT               AA^CGTT
SapI         1250  GAAGAGC              GCTCTTC(1/4)
SexAI         601  ACCAGGT              A^CC[AT]GGT
SfoI           90  GGCGCC               GGC^GCC
SfuI          524  TTCGAA               TT^CGAA
SnaBI         459  TACGTA               TAC^GTA
SphI          326  GCATGC               GCATG^C
StuI         1687  AGGCCT               AGG^CCT
Tth111I      2487  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccIII        643  TCCGGA               T^CCGGA
             1407  TCCGGA              
AcyI           90  GGCGCC               G[GA]^CG[CT]C
              831  GACGCC              
BlpI         1639  GCTGAGC              GC^T[ACGT]AGC
             2535  GCTTAGC             
Bpu1102I     1639  GCTGAGC              GC^T[ACGT]AGC
             2535  GCTTAGC             
BsaHI          90  GGCGCC               G[GA]^CG[CT]C
              831  GACGCC              
BsaWI         643  TCCGGA               [AT]^CCGG[AT]
             1407  TCCGGA              
BseAI         643  TCCGGA               T^CCGGA
             1407  TCCGGA              
BspEI         643  TCCGGA               T^CCGGA
             1407  TCCGGA              
BspMI        1646  ACCTGC               ACCTGC(4/8)
             2528  ACCTGC              
BstAPI        326  GCATGCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2523  GCACCACCTGC         
BstYI        1049  AGATCC               [GA]^GATC[CT]
             2988  AGATCT              
CelII        1639  GCTGAGC              GC^T[ACGT]AGC
             2535  GCTTAGC             
EcoNI        1690  CCTTACCTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2739  CCTGGAACAGG         
Hin1I          90  GGCGCC               G[GA]^CG[CT]C
              831  GACGCC              
MaeII         460  ACGT                 A^CGT
             1308  ACGT                
MflI         1049  AGATCC               [GA]^GATC[CT]
             2988  AGATCT              
MroI          643  TCCGGA               T^CCGGA
             1407  TCCGGA              
NspI          326  GCATGC               [GA]CATG^[CT]
             3197  ACATGT              
PflMI        3150  CCAGGCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3244  CCAGAAGATGG         
PstI          152  CTGCAG               CTGCA^G
             2639  CTGCAG              
Van91I       3150  CCAGGCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3244  CCAGAAGATGG         
XhoII        1049  AGATCC               [GA]^GATC[CT]
             2988  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
Alw44I      AlwI        AlwNI       ApaLI       ApoI        
AspHI       AvaII       BanII       BbsI        BbvI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaI        BsaJI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrBI       BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       EaeI        EarI        EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     
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