Restriction map for pF1KA0987
 (Linear) (Six-base) MAPSORT of: KIAA0987.seq  Check: 1559  from: 1  to: 3261



KIAA0987.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1655    2537    3261 
   Size:       1655     882     724

  Fragments arranged by size: 

                1655     882     724
 
AclI AA'CG_TT

Cuts at:      0    1308    3261 
   Size:       1308    1953
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     611    2363    2379    3261 
   Size:        611    1752      16     882

  Fragments arranged by size: 

                1752     882     611      16
 
AflIII A'CryG_T

Cuts at:      0    3197    3261 
   Size:       3197      64
 
AhdI GACnn_n'nnGTC

Cuts at:      0     639    3261 
   Size:        639    2622
 
AleI CACnn'nnGTG

Cuts at:      0     169    1399    2597    3261 
   Size:        169    1230    1198     664

  Fragments arranged by size: 

                1230    1198     664     169
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     187    2330    3261 
   Size:        187    2143     931

  Fragments arranged by size: 

                2143     931     187
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2321    2353    3261 
   Size:       2321      32     908

  Fragments arranged by size: 

                2321     908      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     132     577    2245    2761    3156    3261 
   Size:        132     445    1668     516     395     105

  Fragments arranged by size: 

                1668     516     445     395     132     105
 
ApaLI G'TGCA_C

Cuts at:      0    2521    2590    3014    3261 
   Size:       2521      69     424     247

  Fragments arranged by size: 

                2521     424     247      69
 
ApoI r'AATT_y

Cuts at:      0     559    1192    2428    2906    3261 
   Size:        559     633    1236     478     355

  Fragments arranged by size: 

                1236     633     559     478     355
 
AvaI C'yCGr_G

Cuts at:      0    2043    3261 
   Size:       2043    1218
 
AvrII C'CTAG_G

Cuts at:      0    1695    3261 
   Size:       1695    1566
 
BanI G'GyrC_C

Cuts at:      0      90    3261 
   Size:         90    3171
 
BanII G_rGCy'C

Cuts at:      0      56     688     710    1583    1661    2006    2147    2683 
   Size:         56     632      22     873      78     345     141     536

Cuts at:   2683    3261 
   Size:        578

  Fragments arranged by size: 

                 873     632     578     536     345     141      78      56
                  22
 
BbeI G_GCGC'C

Cuts at:      0      94    3261 
   Size:         94    3167
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     254     797    2351    2860    3261 
   Size:        254     543    1554     509     401

  Fragments arranged by size: 

                1554     543     509     401     254
 
BbvCI CC'TCA_GC

Cuts at:      0    1421    3261 
   Size:       1421    1840
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2904    2938    3261 
   Size:       2904      34     323

  Fragments arranged by size: 

                2904     323      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1804    3261 
   Size:       1804    1457
 
BclI T'GATC_A

Cuts at:      0    1391    3261 
   Size:       1391    1870
 
BfrBI ATG'CAT

Cuts at:      0     852    3261 
   Size:        852    2409
 
BglII A'GATC_T

Cuts at:      0    2988    3261 
   Size:       2988     273
 
BlpI GC'TnA_GC

Cuts at:      0    1640    2536    3261 
   Size:       1640     896     725

  Fragments arranged by size: 

                1640     896     725
 
Bme1580I G_kGCm'C

Cuts at:      0    1563    1901    2525    2594    3018    3261 
   Size:       1563     338     624      69     424     243

  Fragments arranged by size: 

                1563     624     424     338     243      69
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     402    3261 
   Size:        402    2859
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     372    1673    2020    3013    3261 
   Size:        154     218    1301     347     993     248

  Fragments arranged by size: 

                1301     993     347     248     218     154
 
Bpu10I CC'TnA_GC

Cuts at:      0     503     908    1421    3161    3261 
   Size:        503     405     513    1740     100

  Fragments arranged by size: 

                1740     513     503     405     100
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2040    2131    2156    3261 
   Size:       2040      91      25    1105

  Fragments arranged by size: 

                2040    1105      91      25
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     299    2098    2554    3261 
   Size:        299    1799     456     707

  Fragments arranged by size: 

                1799     707     456     299
 
BsaAI yAC'GTr

Cuts at:      0     461    3261 
   Size:        461    2800
 
BsaBI GATnn'nnATC

Cuts at:      0     899    3261 
   Size:        899    2362
 
BsaHI Gr'CG_yC

Cuts at:      0      91     832    3261 
   Size:         91     741    2429

  Fragments arranged by size: 

                2429     741      91
 
BsaWI w'CCGG_w

Cuts at:      0     643    1407    3261 
   Size:        643     764    1854

  Fragments arranged by size: 

                1854     764     643
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1389    1419    2551    2581    3261 
   Size:       1389      30    1132      30     680

  Fragments arranged by size: 

                1389    1132     680      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     130    1438    1441    2590    2734    2797    2906    3261 
   Size:        130    1308       3    1149     144      63     109     355

  Fragments arranged by size: 

                1308    1149     355     144     130     109      63       3
            
 
BseYI C'CCAG_C

Cuts at:      0     681    1275    2462    3261 
   Size:        681     594    1187     799

  Fragments arranged by size: 

                1187     799     681     594
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     793    2575    2813    3261 
   Size:        793    1782     238     448

  Fragments arranged by size: 

                1782     793     448     238
 
BsiEI CG_ry'CG

Cuts at:      0       8     975    1057    3261 
   Size:          8     967      82    2204

  Fragments arranged by size: 

                2204     967      82       8
 
BsiHKAI G_wGCw'C

Cuts at:      0     710    1583    1646    2147    2525    2594    3018    3261 
   Size:        710     873      63     501     378      69     424     243

  Fragments arranged by size: 

                 873     710     501     424     378     243      69      63
            
 
BsmI GAATG_Cn'

Cuts at:      0    2653    3261 
   Size:       2653     608
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2500    3261 
   Size:       2500     761
 
Bsp1286I G_dGCh'C

Cuts at:      0      56     688     710    1563    1583    1646    1661    1901 
   Size:         56     632      22     853      20      63      15     240

Cuts at:   1901    2006    2147    2525    2594    2683    3018    3261 
   Size:        105     141     378      69      89     335     243

  Fragments arranged by size: 

                 853     632     378     335     243     240     141     105
                  89      69      63      56      22      20      15
 
BspEI T'CCGG_A

Cuts at:      0     643    1407    3261 
   Size:        643     764    1854

  Fragments arranged by size: 

                1854     764     643
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1655    2537    3261 
   Size:       1655     882     724

  Fragments arranged by size: 

                1655     882     724
 
BsrBI CCG'CTC

Cuts at:      0    1431    1883    2580    3261 
   Size:       1431     452     697     681

  Fragments arranged by size: 

                1431     697     681     452
 
BsrFI r'CCGG_y

Cuts at:      0     974    3261 
   Size:        974    2287
 
BssHII G'CGCG_C

Cuts at:      0      81    3261 
   Size:         81    3180
 
BssSI C'ACGA_G

Cuts at:      0    1495    3261 
   Size:       1495    1766
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     332    2529    3261 
   Size:        332    2197     732

  Fragments arranged by size: 

                2197     732     332
 
BstBI TT'CG_AA

Cuts at:      0     525    3261 
   Size:        525    2736
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3216    3261 
   Size:       3216      45
 
BstYI r'GATC_y

Cuts at:      0    1049    2988    3261 
   Size:       1049    1939     273

  Fragments arranged by size: 

                1939    1049     273
 
BtsI GCAGTG_nn'

Cuts at:      0     337    2243    2883    3261 
   Size:        337    1906     640     378

  Fragments arranged by size: 

                1906     640     378     337
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2355    3261 
   Size:       2355     906
 
EaeI y'GGCC_r

Cuts at:      0    1054    1350    1484    2297    3261 
   Size:       1054     296     134     813     964

  Fragments arranged by size: 

                1054     964     813     296     134
 
EagI C'GGCC_G

Cuts at:      0    1054    3261 
   Size:       1054    2207
 
EarI CTCTTCn'nnn_

Cuts at:      0    1245    1436    1915    2057    2263    2339    2744    3261 
   Size:       1245     191     479     142     206      76     405     517

  Fragments arranged by size: 

                1245     517     479     405     206     191     142      76
            
 
EcoICRI GAG'CTC

Cuts at:      0     708    1581    2145    3261 
   Size:        708     873     564    1116

  Fragments arranged by size: 

                1116     873     708     564
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     372     611    1673    2020    2363    2379    3013 
   Size:        154     218     239    1062     347     343      16     634

Cuts at:   3013    3261 
   Size:        248

  Fragments arranged by size: 

                1062     634     347     343     248     239     218     154
                  16
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1694    2743    3261 
   Size:       1694    1049     518

  Fragments arranged by size: 

                1694    1049     518
 
EcoO109I rG'GnC_Cy

Cuts at:      0     129    3261 
   Size:        129    3132
 
EcoRI G'AATT_C

Cuts at:      0    2428    3261 
   Size:       2428     833
 
EcoRV GAT'ATC

Cuts at:      0     597    3261 
   Size:        597    2664
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     361     551    3261 
   Size:        361     190    2710

  Fragments arranged by size: 

                2710     361     190
 
HaeII r_GCGC'y

Cuts at:      0      94    2027    3157    3261 
   Size:         94    1933    1130     104

  Fragments arranged by size: 

                1933    1130     104      94
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      13      45     620     625     652     657    1489    1521 
   Size:         13      32     575       5      27       5     832      32

Cuts at:   1521    2175    2207    3261 
   Size:        654      32    1054

  Fragments arranged by size: 

                1054     832     654     575      32      32      32      27
                  13       5       5
 
KasI G'GCGC_C

Cuts at:      0      90    3261 
   Size:         90    3171
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     473    2102    3261 
   Size:        473    1629    1159

  Fragments arranged by size: 

                1629    1159     473
 
MscI TGG'CCA

Cuts at:      0    1352    3261 
   Size:       1352    1909
 
MslI CAynn'nnrTG

Cuts at:      0     169    1399    2597    3202    3261 
   Size:        169    1230    1198     605      59

  Fragments arranged by size: 

                1230    1198     605     169      59
 
MspA1I CmG'CkG

Cuts at:      0     151    2630    2761    3261 
   Size:        151    2479     131     500

  Fragments arranged by size: 

                2479     500     151     131
 
NarI GG'CG_CC

Cuts at:      0      91    3261 
   Size:         91    3170
 
NdeI CA'TA_TG

Cuts at:      0    2958    3261 
   Size:       2958     303
 
NruI TCG'CGA

Cuts at:      0     971    3261 
   Size:        971    2290
 
NsiI A_TGCA'T

Cuts at:      0     854    3261 
   Size:        854    2407
 
NspI r_CATG'y

Cuts at:      0     330    3201    3261 
   Size:        330    2871      60

  Fragments arranged by size: 

                2871     330      60
 
PasI CC'CwG_GG

Cuts at:      0    1681    3261 
   Size:       1681    1580
 
PciI A'CATG_T

Cuts at:      0    3197    3261 
   Size:       3197      64
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3156    3250    3261 
   Size:       3156      94      11

  Fragments arranged by size: 

                3156      94      11
 
PfoI T'CCnGG_A

Cuts at:      0    2738    3261 
   Size:       2738     523
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2037    2069    3261 
   Size:       2037      32    1192

  Fragments arranged by size: 

                2037    1192      32
 
PshAI GACnn'nnGTC

Cuts at:      0    1529    3261 
   Size:       1529    1732
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1024    1056    3261 
   Size:       1024      32    2205

  Fragments arranged by size: 

                2205    1024      32
 
PstI C_TGCA'G

Cuts at:      0     156    2643    3261 
   Size:        156    2487     618

  Fragments arranged by size: 

                2487     618     156
 
SacI G_AGCT'C

Cuts at:      0     710    1583    2147    3261 
   Size:        710     873     564    1114

  Fragments arranged by size: 

                1114     873     710     564
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1245    3261 
   Size:       1245    2016
 
SexAI A'CCwGG_T

Cuts at:      0     601    3261 
   Size:        601    2660
 
SfcI C'TryA_G

Cuts at:      0     152    1722    2639    3261 
   Size:        152    1570     917     622

  Fragments arranged by size: 

                1570     917     622     152
 
SfoI GGC'GCC

Cuts at:      0      92    3261 
   Size:         92    3169
 
SmlI C'TyrA_G

Cuts at:      0    2019    2146    2171    3261 
   Size:       2019     127      25    1090

  Fragments arranged by size: 

                2019    1090     127      25
 
SnaBI TAC'GTA

Cuts at:      0     461    3261 
   Size:        461    2800
 
SphI G_CATG'C

Cuts at:      0     330    3261 
   Size:        330    2931
 
StuI AGG'CCT

Cuts at:      0    1689    3261 
   Size:       1689    1572
 
StyI C'CwwG_G

Cuts at:      0     110    1004    1202    1664    1695    2006    3261 
   Size:        110     894     198     462      31     311    1255

  Fragments arranged by size: 

                1255     894     462     311     198     110      31
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      22     281    1567    3261 
   Size:         22     259    1286    1694

  Fragments arranged by size: 

                1694    1286     259      22
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1122    1154    1389    1421    3261 
   Size:       1122      32     235      32    1840

  Fragments arranged by size: 

                1840    1122     235      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2490    3261 
   Size:       2490     771
 

 Enzymes that do cut:

     AarI     AclI     AcuI   AflIII     AhdI     AleI     AlfI     AloI
    AlwNI    ApaLI     ApoI     AvaI    AvrII     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI   BssHII
    BssSI   BstAPI    BstBI    BstXI    BstYI     BtsI     DrdI     EaeI
     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI    HaeII    Hin4I     KasI     MmeI     MscI     MslI   MspA1I
     NarI     NdeI     NruI     NsiI     NspI     PasI     PciI    PflMI
     PfoI     PpiI    PshAI     PsrI     PstI     SacI     SapI    SexAI
     SfcI     SfoI     SmlI    SnaBI     SphI     StuI     StyI    TaqII
     TstI  Tth111I

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AfeI    AflII     AgeI     ApaI     AscI
     AseI    AsiSI     BaeI    BamHI     BglI    BmgBI     BmrI     BmtI
    BsiWI    BspHI    BsrDI    BsrGI   BstEII  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     EciI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     NaeI
     NcoI   NgoMIV     NheI     NotI     PacI     PmeI     PmlI    PpuMI
     PsiI   PspOMI    PspXI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmaI     SpeI
     SrfI     SspI     SwaI     TatI     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI
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