Restriction map for pF1KA0966
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# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       AscI        Asp718I     AspEI       AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BcgI        BciVI       BclI        
BfrI        BlnI        BlpI        Bpu1102I    BsaBI       
BsaHI       BsaWI       BseAI       BsiWI       BsoBI       
Bsp1407I    BspEI       BspHI       BspMI       BsrBI       
BsrFI       BsrGI       Bst1107I    BstEII      BstPI       
BstZ17I     CelII       Cfr10I      CpoI        DraI        
DraIII      EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO65I     EcoRV       EcoT22I     FbaI        
FseI        FspI        HgaI        Hin1I       KasI        
KpnI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MunI        NaeI        NarI        
NdeI        NgoMIV      NotI        NsiI        PacI        
PaeR7I      PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PvuI        
RcaI        RsrII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       StuI        
SwaI        Van91I      XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI         1132  GTAGAC               GT^[AC][GT]AC
AflIII       2549  ACATGT               A^C[GA][CT]GT
ApaI         1862  GGGCCC               GGGCC^C
AseI         3328  ATTAAT               AT^TAAT
AsnI         3328  ATTAAT               AT^TAAT
Asp700I       501  GAAGATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1997  GACTAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI        2687  CCTCAGC              CCTCAGC(-5/-2)
BcnI         1464  CCCGG                CC^[GC]GG
BsaAI        1948  TACGTG               [CT]AC^GT[GA]
BsiEI         591  CGGTCG               CG[GA][CT]^CG
BsmBI        1907  GAGACG               CGTCTC(1/5)
BspDI        1016  ATCGAT               AT^CGAT
BspLU11I     2549  ACATGT               A^CATGT
BssSI         977  CACGAG               CACGAG(-5/-1)
BstAPI       1179  GCAAGTGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI         1016  ATCGAT               AT^CGAT
DrdI          238  GACCATGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         3028  CGGCCA               [CT]^GGCC[GA]
EcoNI          33  CCTGCAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        2584  GAATTC               G^AATTC
FauI           94  CCCGC                CCCGC(4/6)
HaeII        2691  AGCGCC               [GA]GCGC^[CT]
HapII        1465  CCGG                 C^CGG
HincII       2437  GTTAAC               GT[CT]^[GA]AC
HindII       2437  GTTAAC               GT[CT]^[GA]AC
HindIII      1754  AAGCTT               A^AGCTT
HpaI         2437  GTTAAC               GTT^AAC
HpaII        1465  CCGG                 C^CGG
KspI         1033  CCGCGG               CCGC^GG
MspI         1465  CCGG                 C^CGG
NciI         1464  CCCGG                CC^[GC]GG
NheI         2622  GCTAGC               G^CTAGC
NruI         1415  TCGCGA               TCG^CGA
PflFI        1997  GACTAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI        3328  ATTAAT               AT^TAAT
SacII        1033  CCGCGG               CCGC^GG
SspI         2386  AATATT               AAT^ATT
Tth111I      1997  GACTAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         2004  TCTAGA               T^CTAGA
XmnI          501  GAAGATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AfaI          668  GTAC                 GT^AC
             2836  GTAC                
AocI          295  CCTCAGG              CC^T[ACGT]AGG
              787  CCTCAGG             
BglI           68  GCCGCGACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              597  GCCCCTCTGGC         
BseRI         477  GAGGAG               GAGGAG(10/8)
              887  GAGGAG              
BsrDI        2120  CATTGC               GCAATG(2/0)
             2175  CATTGC              
BssHII         48  GCGCGC               G^CGCGC
               50  GCGCGC              
BstBI        1084  TTCGAA               TT^CGAA
             3221  TTCGAA              
Bsu36I        295  CCTCAGG              CC^T[ACGT]AGG
              787  CCTCAGG             
DraII        1862  GGGCCCT              [GA]G^G[ACGT]CC[CT]
             2031  AGGCCCT             
Eco81I        295  CCTCAGG              CC^T[ACGT]AGG
              787  CCTCAGG             
EcoO109I     1862  GGGCCCT              [GA]G^G[ACGT]CC[CT]
             2031  AGGCCCT             
NspV         1084  TTCGAA               TT^CGAA
             3221  TTCGAA              
PstI           34  CTGCAG               CTGCA^G
             2188  CTGCAG              
PvuII        1438  CAGCTG               CAG^CTG
             1556  CAGCTG              
RsaI          668  GTAC                 GT^AC
             2836  GTAC                
SacI            4  GAGCTC               GAGCT^C
              313  GAGCTC              
SapI         2250  GAAGAGC              GCTCTTC(1/4)
                6  GCTCTTC             
SfuI         1084  TTCGAA               TT^CGAA
             3221  TTCGAA              
SphI         2072  GCATGC               GCATG^C
             3134  GCATGC              
XcmI         1412  CCATCGCGAGAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2198  CCAAGCAAGCCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   87 enzymes) #
#########################################

AccII       AciI        AcsI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaII       BanII       
BbsI        BbvI        BfaI        BglII       BmyI        
BpmI        BpuAI       BsaI        BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DsaI        
EarI        EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HhaI        HinP1I      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MvaI        MvnI        MwoI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PleI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       
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