Restriction map for pF1KA0966
 (Linear) (Six-base) MAPSORT of: KIAA0966.seq  Check: 8874  from: 1  to: 3396



KIAA0966.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:21  ..

AccI GT'mk_AC

Cuts at:      0    1133    3396 
   Size:       1133    2263
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412     520    1195    1585    2152    2165    2540    2738 
   Size:        412     108     675     390     567      13     375     198

Cuts at:   2738    3161    3396 
   Size:        423     235

  Fragments arranged by size: 

                 675     567     423     412     390     375     235     198
                 108      13
 
AflIII A'CryG_T

Cuts at:      0    2549    3396 
   Size:       2549     847
 
AleI CACnn'nnGTG

Cuts at:      0    1396    2512    3396 
   Size:       1396    1116     884

  Fragments arranged by size: 

                1396    1116     884
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2095    3116    3396 
   Size:       2095    1021     280

  Fragments arranged by size: 

                2095    1021     280
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1440    2539    3319    3396 
   Size:       1440    1099     780      77

  Fragments arranged by size: 

                1440    1099     780      77
 
ApaI G_GGCC'C

Cuts at:      0    1866    3396 
   Size:       1866    1530
 
ApoI r'AATT_y

Cuts at:      0     161    2322    2338    2584    2595    3396 
   Size:        161    2161      16     246      11     801

  Fragments arranged by size: 

                2161     801     246     161      16      11
 
AseI AT'TA_AT

Cuts at:      0    3329    3396 
   Size:       3329      67
 
BanII G_rGCy'C

Cuts at:      0       8     317    1866    3396 
   Size:          8     309    1549    1530

  Fragments arranged by size: 

                1549    1530     309       8
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     400    2276    2546    2618    3396 
   Size:        400    1876     270      72     778

  Fragments arranged by size: 

                1876     778     400     270      72
 
BbvCI CC'TCA_GC

Cuts at:      0    2688    3396 
   Size:       2688     708
 
BglI GCCn_nnn'nGGC

Cuts at:      0      74     603    3396 
   Size:         74     529    2793

  Fragments arranged by size: 

                2793     529      74
 
BglII A'GATC_T

Cuts at:      0     177     651    1716    2932    3396 
   Size:        177     474    1065    1216     464

  Fragments arranged by size: 

                1216    1065     474     464     177
 
Bme1580I G_kGCm'C

Cuts at:      0    1080    1866    3396 
   Size:       1080     786    1530

  Fragments arranged by size: 

                1530    1080     786
 
BmgBI CAC'GTC

Cuts at:      0    1911    3396 
   Size:       1911    1485
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     587    2709    3396 
   Size:        587    2122     687

  Fragments arranged by size: 

                2122     687     587
 
BmtI G_CTAG'C

Cuts at:      0    2626    3396 
   Size:       2626     770
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     656    3296    3396 
   Size:         67     589    2640     100

  Fragments arranged by size: 

                2640     589     100      67
 
Bpu10I CC'TnA_GC

Cuts at:      0    2688    3396 
   Size:       2688     708
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     325    2263    3166    3396 
   Size:        325    1938     903     230

  Fragments arranged by size: 

                1938     903     325     230
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     230     772    2294    2920    3396 
   Size:        230     542    1522     626     476

  Fragments arranged by size: 

                1522     626     542     476     230
 
BsaAI yAC'GTr

Cuts at:      0    1950    3396 
   Size:       1950    1446
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    3156    3186    3396 
   Size:       3156      30     210

  Fragments arranged by size: 

                3156     210      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     492     902    3396 
   Size:        492     410    2494

  Fragments arranged by size: 

                2494     492     410
 
BseYI C'CCAG_C

Cuts at:      0    1330    1549    1859    3396 
   Size:       1330     219     310    1537

  Fragments arranged by size: 

                1537    1330     310     219
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      81     583     736    2065    3396 
   Size:         81     502     153    1329    1331

  Fragments arranged by size: 

                1331    1329     502     153      81
 
BsiEI CG_ry'CG

Cuts at:      0     594    3396 
   Size:        594    2802
 
BsiHKAI G_wGCw'C

Cuts at:      0       8     275     317     577    1238    3396 
   Size:          8     267      42     260     661    2158

  Fragments arranged by size: 

                2158     661     267     260      42       8
 
BsmI GAATG_Cn'

Cuts at:      0     875    1700    3093    3396 
   Size:        875     825    1393     303

  Fragments arranged by size: 

                1393     875     825     303
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1901    3396 
   Size:       1901    1495
 
Bsp1286I G_dGCh'C

Cuts at:      0       8     275     317     577    1080    1238    1866    3396 
   Size:          8     267      42     260     503     158     628    1530

  Fragments arranged by size: 

                1530     628     503     267     260     158      42       8
            
 
BsrDI GCAATG_nn'

Cuts at:      0    2119    2174    3396 
   Size:       2119      55    1222

  Fragments arranged by size: 

                2119    1222      55
 
BssHII G'CGCG_C

Cuts at:      0      48      50    3396 
   Size:         48       2    3346

  Fragments arranged by size: 

                3346      48       2
 
BssSI C'ACGA_G

Cuts at:      0     977    3396 
   Size:        977    2419
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1185    3396 
   Size:       1185    2211
 
BstBI TT'CG_AA

Cuts at:      0    1085    3222    3396 
   Size:       1085    2137     174

  Fragments arranged by size: 

                2137    1085     174
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     132     921    2865    3396 
   Size:        132     789    1944     531

  Fragments arranged by size: 

                1944     789     531     132
 
BstYI r'GATC_y

Cuts at:      0     177     300     651    1716    2932    3396 
   Size:        177     123     351    1065    1216     464

  Fragments arranged by size: 

                1216    1065     464     351     177     123
 
Bsu36I CC'TnA_GG

Cuts at:      0     296     788    3396 
   Size:        296     492    2608

  Fragments arranged by size: 

                2608     492     296
 
BtgI C'CryG_G

Cuts at:      0     184    1033    1851    2207    3230    3270    3396 
   Size:        184     849     818     356    1023      40     126

  Fragments arranged by size: 

                1023     849     818     356     184     126      40
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1178    1398    3017    3396 
   Size:       1178     220    1619     379

  Fragments arranged by size: 

                1619    1178     379     220
 
BtsI GCAGTG_nn'

Cuts at:      0    1236    3293    3396 
   Size:       1236    2057     103

  Fragments arranged by size: 

                2057    1236     103
 
ClaI AT'CG_AT

Cuts at:      0    1017    3396 
   Size:       1017    2379
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2031    2066    3056    3091    3396 
   Size:       2031      35     990      35     305

  Fragments arranged by size: 

                2031     990     305      35      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     244    3396 
   Size:        244    3152
 
EaeI y'GGCC_r

Cuts at:      0    3028    3396 
   Size:       3028     368
 
EarI CTCTTCn'nnn_

Cuts at:      0      13     485     879    1140    2141    2245    3298    3396 
   Size:         13     472     394     261    1001     104    1053      98

  Fragments arranged by size: 

                1053    1001     472     394     261     104      98      13
            
 
EcoICRI GAG'CTC

Cuts at:      0       6     315    3396 
   Size:          6     309    3081

  Fragments arranged by size: 

                3081     309       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      67     412     520     656    1195    1585    2152    2165 
   Size:         67     345     108     136     539     390     567      13

Cuts at:   2165    2540    2738    3161    3296    3396 
   Size:        375     198     423     135     100

  Fragments arranged by size: 

                 567     539     423     390     375     345     198     136
                 135     108     100      67      13
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      37    3396 
   Size:         37    3359
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1863    2032    3396 
   Size:       1863     169    1364

  Fragments arranged by size: 

                1863    1364     169
 
EcoRI G'AATT_C

Cuts at:      0    2584    3396 
   Size:       2584     812
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1204    1590    2182    2485    3396 
   Size:       1204     386     592     303     911

  Fragments arranged by size: 

                1204     911     592     386     303
 
HaeII r_GCGC'y

Cuts at:      0    2695    3396 
   Size:       2695     701
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     505     537     576     608     636     654     668     686 
   Size:        505      32      39      32      28      18      14      18

Cuts at:    686     771     803    1545    1577    1631    1663    1701    1733 
   Size:         85      32     742      32      54      32      38      32

Cuts at:   1733    2551    2583    2917    2949    3009    3041    3396 
   Size:        818      32     334      32      60      32     355

  Fragments arranged by size: 

                 818     742     505     355     334      85      60      54
                  39      38      32      32      32      32      32      32
                  32      32      32      28      18      18      14
 
HincII GTy'rAC

Cuts at:      0    2439    3396 
   Size:       2439     957
 
HindIII A'AGCT_T

Cuts at:      0    1754    3396 
   Size:       1754    1642
 
HpaI GTT'AAC

Cuts at:      0    2439    3396 
   Size:       2439     957
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     114     452    3396 
   Size:        114     338    2944

  Fragments arranged by size: 

                2944     338     114
 
MslI CAynn'nnrTG

Cuts at:      0    1396    1705    2512    2579    2777    3396 
   Size:       1396     309     807      67     198     619

  Fragments arranged by size: 

                1396     807     619     309     198      67
 
MspA1I CmG'CkG

Cuts at:      0    1035    1440    1558    3396 
   Size:       1035     405     118    1838

  Fragments arranged by size: 

                1838    1035     405     118
 
NcoI C'CATG_G

Cuts at:      0     184    1851    2207    3230    3270    3396 
   Size:        184    1667     356    1023      40     126

  Fragments arranged by size: 

                1667    1023     356     184     126      40
 
NheI G'CTAG_C

Cuts at:      0    2622    3396 
   Size:       2622     774
 
NruI TCG'CGA

Cuts at:      0    1417    3396 
   Size:       1417    1979
 
NspI r_CATG'y

Cuts at:      0    2076    2553    3138    3396 
   Size:       2076     477     585     258

  Fragments arranged by size: 

                2076     585     477     258
 
PasI CC'CwG_GG

Cuts at:      0    2313    3396 
   Size:       2313    1083
 
PciI A'CATG_T

Cuts at:      0    2549    3396 
   Size:       2549     847
 
PspOMI G'GGCC_C

Cuts at:      0    1862    3396 
   Size:       1862    1534
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1460    1492    3396 
   Size:       1460      32    1904

  Fragments arranged by size: 

                1904    1460      32
 
PstI C_TGCA'G

Cuts at:      0      38    2192    3396 
   Size:         38    2154    1204

  Fragments arranged by size: 

                2154    1204      38
 
PvuII CAG'CTG

Cuts at:      0    1440    1558    3396 
   Size:       1440     118    1838

  Fragments arranged by size: 

                1838    1440     118
 
SacI G_AGCT'C

Cuts at:      0       8     317    3396 
   Size:          8     309    3079

  Fragments arranged by size: 

                3079     309       8
 
SacII CC_GC'GG

Cuts at:      0    1036    3396 
   Size:       1036    2360
 
SapI GCTCTTCn'nnn_

Cuts at:      0      13    2245    3396 
   Size:         13    2232    1151

  Fragments arranged by size: 

                2232    1151      13
 
SfcI C'TryA_G

Cuts at:      0      34    2085    2188    3396 
   Size:         34    2051     103    1208

  Fragments arranged by size: 

                2051    1208     103      34
 
SmlI C'TyrA_G

Cuts at:      0     304    2242    3145    3396 
   Size:        304    1938     903     251

  Fragments arranged by size: 

                1938     903     304     251
 
SphI G_CATG'C

Cuts at:      0    2076    3138    3396 
   Size:       2076    1062     258

  Fragments arranged by size: 

                2076    1062     258
 
SspI AAT'ATT

Cuts at:      0    2388    3396 
   Size:       2388    1008
 
StyI C'CwwG_G

Cuts at:      0      17     184     705    1736    1851    2207    2298    3230 
   Size:         17     167     521    1031     115     356      91     932

Cuts at:   3230    3270    3396 
   Size:         40     126

  Fragments arranged by size: 

                1031     932     521     356     167     126     115      91
                  40      17
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     635     833    2699    3396 
   Size:        635     198    1866     697

  Fragments arranged by size: 

                1866     697     635     198
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1347    1379    3396 
   Size:       1347      32    2017

  Fragments arranged by size: 

                2017    1347      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2000    3396 
   Size:       2000    1396
 
XbaI T'CTAG_A

Cuts at:      0    2004    3396 
   Size:       2004    1392
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1419    2205    3396 
   Size:       1419     786    1191

  Fragments arranged by size: 

                1419    1191     786
 
XmnI GAAnn'nnTTC

Cuts at:      0     505    3396 
   Size:        505    2891
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI     AlfI    AlwNI     ApaI     ApoI
     AseI    BanII     BbsI    BbvCI     BglI    BglII Bme1580I    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BsrDI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DrdI     EaeI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I
   HincII  HindIII     HpaI     MmeI     MslI   MspA1I     NcoI     NheI
     NruI     NspI     PasI     PciI   PspOMI     PsrI     PstI    PvuII
     SacI    SacII     SapI     SfcI     SmlI     SphI     SspI     StyI
    TaqII     TstI  Tth111I     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AloI    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BcgI    BciVI     BclI    BfrBI     BlpI     BplI
    BsaBI    BsaHI    BsaWI    BsiWI    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BstEII  BstZ17I     DraI   DraIII     EagI     EciI
    EcoRV     FseI     FspI    FspAI     KasI     KpnI     MfeI     MluI
     MscI     NaeI     NarI     NdeI   NgoMIV     NotI     NsiI     PacI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PvuI    RsrII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     StuI     SwaI     TatI     XhoI     XmaI     ZraI
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