Restriction map for pF1KA0947
 (Linear) (Six-base) MAPSORT of: KIAA0947.seq  Check: 5814  from: 1  to: 6798



KIAA0947.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     668    6798 
   Size:        668    6130
 
AatII G_ACGT'C

Cuts at:      0    3460    6798 
   Size:       3460    3338
 
AccI GT'mk_AC

Cuts at:      0    5518    6478    6633    6798 
   Size:       5518     960     155     165

  Fragments arranged by size: 

                5518     960     165     155
 
Acc65I G'GTAC_C

Cuts at:      0    3274    6798 
   Size:       3274    3524
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     643    1658    3200    3842    3929    4694    5093    5384 
   Size:        643    1015    1542     642      87     765     399     291

Cuts at:   5384    5654    5776    6798 
   Size:        270     122    1022

  Fragments arranged by size: 

                1542    1022    1015     765     643     642     399     291
                 270     122      87
 
AflIII A'CryG_T

Cuts at:      0    4302    6101    6798 
   Size:       4302    1799     697

  Fragments arranged by size: 

                4302    1799     697
 
AgeI A'CCGG_T

Cuts at:      0    2686    6798 
   Size:       2686    4112
 
AhdI GACnn_n'nnGTC

Cuts at:      0    5556    6798 
   Size:       5556    1242
 
AleI CACnn'nnGTG

Cuts at:      0    2070    2378    2749    3019    6798 
   Size:       2070     308     371     270    3779

  Fragments arranged by size: 

                3779    2070     371     308     270
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     443    3019    3360    3871    5165    6173    6798 
   Size:        443    2576     341     511    1294    1008     625

  Fragments arranged by size: 

                2576    1294    1008     625     511     443     341
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1744    3412    4096    4947    5410    6798 
   Size:       1744    1668     684     851     463    1388

  Fragments arranged by size: 

                1744    1668    1388     851     684     463
 
ApaI G_GGCC'C

Cuts at:      0    5289    6798 
   Size:       5289    1509
 
ApoI r'AATT_y

Cuts at:      0      84     242     490     520     569     952    1158    2536 
   Size:         84     158     248      30      49     383     206    1378

Cuts at:   2536    2841    3393    3816    3891    5238    5352    5872    5936 
   Size:        305     552     423      75    1347     114     520      64

Cuts at:   5936    6292    6310    6689    6798 
   Size:        356      18     379     109

  Fragments arranged by size: 

                1378    1347     552     520     423     383     379     356
                 305     248     206     158     114     109      84      75
                  64      49      30      18
 
AseI AT'TA_AT

Cuts at:      0     109    1706    2311    6137    6798 
   Size:        109    1597     605    3826     661

  Fragments arranged by size: 

                3826    1597     661     605     109
 
AvaI C'yCGr_G

Cuts at:      0      31    1071    1324    1514    6798 
   Size:         31    1040     253     190    5284

  Fragments arranged by size: 

                5284    1040     253     190      31
 
BanI G'GyrC_C

Cuts at:      0      27    2372    3193    3274    6798 
   Size:         27    2345     821      81    3524

  Fragments arranged by size: 

                3524    2345     821      81      27
 
BanII G_rGCy'C

Cuts at:      0     859    2122    2282    5289    6030    6798 
   Size:        859    1263     160    3007     741     768

  Fragments arranged by size: 

                3007    1263     859     768     741     160
 
BbeI G_GCGC'C

Cuts at:      0      31    6798 
   Size:         31    6767
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     505    1079    1501    1931    2446    4088    6326    6798 
   Size:        505     574     422     430     515    1642    2238     472

  Fragments arranged by size: 

                2238    1642     574     515     505     472     430     422
            
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1505    1539    6798 
   Size:       1505      34    5259

  Fragments arranged by size: 

                5259    1505      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     324    3243    5276    6798 
   Size:        324    2919    2033    1522

  Fragments arranged by size: 

                2919    2033    1522     324
 
BclI T'GATC_A

Cuts at:      0     960     966    2148    2333    4578    4611    6798 
   Size:        960       6    1182     185    2245      33    2187

  Fragments arranged by size: 

                2245    2187    1182     960     185      33       6
 
BfrBI ATG'CAT

Cuts at:      0    1526    6798 
   Size:       1526    5272
 
BglII A'GATC_T

Cuts at:      0     984    2970    3076    6798 
   Size:        984    1986     106    3722

  Fragments arranged by size: 

                3722    1986     984     106
 
Bme1580I G_kGCm'C

Cuts at:      0    1043    5289    6777    6798 
   Size:       1043    4246    1488      21

  Fragments arranged by size: 

                4246    1488    1043      21
 
BmgBI CAC'GTC

Cuts at:      0    5068    6798 
   Size:       5068    1730
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     283    1929    4381    5020    5435    5564    6798 
   Size:        283    1646    2452     639     415     129    1234

  Fragments arranged by size: 

                2452    1646    1234     639     415     283     129
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     398    4005    6798 
   Size:        398    3607    2793

  Fragments arranged by size: 

                3607    2793     398
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     702     843    2801    2964    4355    5063    5612    6798 
   Size:        702     141    1958     163    1391     708     549    1186

  Fragments arranged by size: 

                1958    1391    1186     708     702     549     163     141
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     404    6778    6798 
   Size:        404    6374      20

  Fragments arranged by size: 

                6374     404      20
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     931    1781    2136    2652    3556    6216    6687    6798 
   Size:        931     850     355     516     904    2660     471     111

  Fragments arranged by size: 

                2660     931     904     850     516     471     355     111
            
 
BsaI GGTCTCn'nnnn_

Cuts at:      0       7    3423    6798 
   Size:          7    3416    3375

  Fragments arranged by size: 

                3416    3375       7
 
BsaBI GATnn'nnATC

Cuts at:      0     335     965     983    4241    6555    6798 
   Size:        335     630      18    3258    2314     243

  Fragments arranged by size: 

                3258    2314     630     335     243      18
 
BsaHI Gr'CG_yC

Cuts at:      0      28    3457    6798 
   Size:         28    3429    3341

  Fragments arranged by size: 

                3429    3341      28
 
BsaWI w'CCGG_w

Cuts at:      0    2067    2686    6066    6798 
   Size:       2067     619    3380     732

  Fragments arranged by size: 

                3380    2067     732     619
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     732     762    3066    3096    4818    4848    6798 
   Size:        732      30    2304      30    1722      30    1950

  Fragments arranged by size: 

                2304    1950    1722     732      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     239    1013    3921    4901    5066    5126    6798 
   Size:        239     774    2908     980     165      60    1672

  Fragments arranged by size: 

                2908    1672     980     774     239     165      60
 
BseYI C'CCAG_C

Cuts at:      0    6673    6798 
   Size:       6673     125
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     805    1535    2950    5705    6798 
   Size:        805     730    1415    2755    1093

  Fragments arranged by size: 

                2755    1415    1093     805     730
 
BsiHKAI G_wGCw'C

Cuts at:      0     859    2282    4120    4507    6030    6784    6798 
   Size:        859    1423    1838     387    1523     754      14

  Fragments arranged by size: 

                1838    1523    1423     859     754     387      14
 
BsmI GAATG_Cn'

Cuts at:      0     370    3296    6228    6798 
   Size:        370    2926    2932     570

  Fragments arranged by size: 

                2932    2926     570     370
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3305    5138    6798 
   Size:       3305    1833    1660

  Fragments arranged by size: 

                3305    1833    1660
 
Bsp1286I G_dGCh'C

Cuts at:      0     859    1043    2122    2282    4120    4507    5289    6030 
   Size:        859     184    1079     160    1838     387     782     741

Cuts at:   6030    6777    6784    6798 
   Size:        747       7      14

  Fragments arranged by size: 

                1838    1079     859     782     747     741     387     184
                 160      14       7
 
BspEI T'CCGG_A

Cuts at:      0    6066    6798 
   Size:       6066     732
 
BspHI T'CATG_A

Cuts at:      0     963    6798 
   Size:        963    5835
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     668     705    4094    5373    6798 
   Size:        668      37    3389    1279    1425

  Fragments arranged by size: 

                3389    1425    1279     668      37
 
BsrDI GCAATG_nn'

Cuts at:      0    2257    2471    3837    6152    6470    6798 
   Size:       2257     214    1366    2315     318     328

  Fragments arranged by size: 

                2315    2257    1366     328     318     214
 
BsrFI r'CCGG_y

Cuts at:      0    2686    6798 
   Size:       2686    4112
 
BssSI C'ACGA_G

Cuts at:      0     350    5327    6798 
   Size:        350    4977    1471

  Fragments arranged by size: 

                4977    1471     350
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    5410    6472    6798 
   Size:       5410    1062     326

  Fragments arranged by size: 

                5410    1062     326
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2973    6702    6798 
   Size:       2973    3729      96

  Fragments arranged by size: 

                3729    2973      96
 
BstYI r'GATC_y

Cuts at:      0     277     984    2265    2970    3076    5082    6386    6698 
   Size:        277     707    1281     705     106    2006    1304     312

Cuts at:   6698    6798 
   Size:        100

  Fragments arranged by size: 

                2006    1304    1281     707     705     312     277     106
                 100
 
BstZ17I GTA'TAC

Cuts at:      0    6479    6634    6798 
   Size:       6479     155     164

  Fragments arranged by size: 

                6479     164     155
 
Bsu36I CC'TnA_GG

Cuts at:      0    2960    5231    6798 
   Size:       2960    2271    1567

  Fragments arranged by size: 

                2960    2271    1567
 
BtgI C'CryG_G

Cuts at:      0     809    5047    6625    6798 
   Size:        809    4238    1578     173

  Fragments arranged by size: 

                4238    1578     809     173
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      69    6798 
   Size:         69    6729
 
BtsI GCAGTG_nn'

Cuts at:      0    2166    2546    2933    3003    3354    4500    5585    6798 
   Size:       2166     380     387      70     351    1146    1085    1213

  Fragments arranged by size: 

                2166    1213    1146    1085     387     380     351      70
            
 
ClaI AT'CG_AT

Cuts at:      0    4662    6798 
   Size:       4662    2136
 
DraI TTT'AAA

Cuts at:      0     284     317    2039    3896    6798 
   Size:        284      33    1722    1857    2902

  Fragments arranged by size: 

                2902    1857    1722     284      33
 
DraIII CAC_nnn'GTG

Cuts at:      0     222    3758    4322    4324    5200    6798 
   Size:        222    3536     564       2     876    1598

  Fragments arranged by size: 

                3536    1598     876     564     222       2
 
EaeI y'GGCC_r

Cuts at:      0    1746    4998    5050    5153    5184    5208    6798 
   Size:       1746    3252      52     103      31      24    1590

  Fragments arranged by size: 

                3252    1746    1590     103      52      31      24
 
EarI CTCTTCn'nnn_

Cuts at:      0     189     221     293     367    1031    1902    2149    3692 
   Size:        189      32      72      74     664     871     247    1543

Cuts at:   3692    6798 
   Size:       3106

  Fragments arranged by size: 

                3106    1543     871     664     247     189      74      72
                  32
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      58    5324    6729    6798 
   Size:         58    5266    1405      69

  Fragments arranged by size: 

                5266    1405      69      58
 
EcoICRI GAG'CTC

Cuts at:      0     857    2280    6028    6798 
   Size:        857    1423    3748     770

  Fragments arranged by size: 

                3748    1423     857     770
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     643     702     843    1658    2801    2964    3200    3842 
   Size:        643      59     141     815    1143     163     236     642

Cuts at:   3842    3929    4355    4694    5063    5093    5384    5612    5654 
   Size:         87     426     339     369      30     291     228      42

Cuts at:   5654    5776    6798 
   Size:        122    1022

  Fragments arranged by size: 

                1143    1022     815     643     642     426     369     339
                 291     236     228     163     141     122      87      59
                  42      30
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     668    6798 
   Size:        668    6130
 
EcoO109I rG'GnC_Cy

Cuts at:      0     773    3090    4885    5058    5227    5285    5362    5558 
   Size:        773    2317    1795     173     169      58      77     196

Cuts at:   5558    6798 
   Size:       1240

  Fragments arranged by size: 

                2317    1795    1240     773     196     173     169      77
                  58
 
EcoRI G'AATT_C

Cuts at:      0    2536    5238    6798 
   Size:       2536    2702    1560

  Fragments arranged by size: 

                2702    2536    1560
 
EcoRV GAT'ATC

Cuts at:      0    4961    5948    6798 
   Size:       4961     987     850

  Fragments arranged by size: 

                4961     987     850
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     250     843    3700    6798 
   Size:        250     593    2857    3098

  Fragments arranged by size: 

                3098    2857     593     250
 
HaeII r_GCGC'y

Cuts at:      0      31    6798 
   Size:         31    6767
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1126    1158    1522    1554    1825    1857    2290    2322 
   Size:       1126      32     364      32     271      32     433      32

Cuts at:   2322    2433    2465    2962    2994    3447    3479    4551    4583 
   Size:        111      32     497      32     453      32    1072      32

Cuts at:   4583    4956    4988    5542    5574    6798 
   Size:        373      32     554      32    1224

  Fragments arranged by size: 

                1224    1126    1072     554     497     453     433     373
                 364     271     111      32      32      32      32      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     152    2696    5513    6798 
   Size:        152    2544    2817    1285

  Fragments arranged by size: 

                2817    2544    1285     152
 
HindIII A'AGCT_T

Cuts at:      0    6707    6798 
   Size:       6707      91
 
HpaI GTT'AAC

Cuts at:      0     152    6798 
   Size:        152    6646
 
KasI G'GCGC_C

Cuts at:      0      27    6798 
   Size:         27    6771
 
KpnI G_GTAC'C

Cuts at:      0    3278    6798 
   Size:       3278    3520
 
MfeI C'AATT_G

Cuts at:      0     998    6532    6798 
   Size:        998    5534     266

  Fragments arranged by size: 

                5534     998     266
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     775    6798 
   Size:        775    6023
 
MscI TGG'CCA

Cuts at:      0    1748    5000    5052    5210    6798 
   Size:       1748    3252      52     158    1588

  Fragments arranged by size: 

                3252    1748    1588     158      52
 
MslI CAynn'nnrTG

Cuts at:      0    2070    2378    2749    2996    3019    4182    4387    4739 
   Size:       2070     308     371     247      23    1163     205     352

Cuts at:   4739    5810    6798 
   Size:       1071     988

  Fragments arranged by size: 

                2070    1163    1071     988     371     352     308     247
                 205      23
 
MspA1I CmG'CkG

Cuts at:      0    3059    3967    4001    5632    6755    6798 
   Size:       3059     908      34    1631    1123      43

  Fragments arranged by size: 

                3059    1631    1123     908      43      34
 
NarI GG'CG_CC

Cuts at:      0      28    6798 
   Size:         28    6770
 
NcoI C'CATG_G

Cuts at:      0     809    5047    6625    6798 
   Size:        809    4238    1578     173

  Fragments arranged by size: 

                4238    1578     809     173
 
NdeI CA'TA_TG

Cuts at:      0     585    6798 
   Size:        585    6213
 
NruI TCG'CGA

Cuts at:      0      54    6798 
   Size:         54    6744
 
NsiI A_TGCA'T

Cuts at:      0    1528    6798 
   Size:       1528    5270
 
NspI r_CATG'y

Cuts at:      0     794    3943    4306    6105    6466    6607    6798 
   Size:        794    3149     363    1799     361     141     191

  Fragments arranged by size: 

                3149    1799     794     363     361     191     141
 
PasI CC'CwG_GG

Cuts at:      0    1573    5614    6798 
   Size:       1573    4041    1184

  Fragments arranged by size: 

                4041    1573    1184
 
PciI A'CATG_T

Cuts at:      0    4302    6101    6798 
   Size:       4302    1799     697

  Fragments arranged by size: 

                4302    1799     697
 
PfoI T'CCnGG_A

Cuts at:      0    4332    6701    6798 
   Size:       4332    2369      97

  Fragments arranged by size: 

                4332    2369      97
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     620     652    6798 
   Size:        620      32    6146

  Fragments arranged by size: 

                6146     620      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     773    5058    5362    5558    6798 
   Size:        773    4285     304     196    1240

  Fragments arranged by size: 

                4285    1240     773     304     196
 
PshAI GACnn'nnGTC

Cuts at:      0    6668    6798 
   Size:       6668     130
 
PsiI TTA'TAA

Cuts at:      0    4216    4228    4625    6798 
   Size:       4216      12     397    2173

  Fragments arranged by size: 

                4216    2173     397      12
 
PspOMI G'GGCC_C

Cuts at:      0    5285    6798 
   Size:       5285    1513
 
PspXI vC'TCGA_Gb

Cuts at:      0    1514    6798 
   Size:       1514    5284
 
PstI C_TGCA'G

Cuts at:      0      80     185    2057    2928    3349    5106    5144    5592 
   Size:         80     105    1872     871     421    1757      38     448

Cuts at:   5592    5983    6798 
   Size:        391     815

  Fragments arranged by size: 

                1872    1757     871     815     448     421     391     105
                  80      38
 
PvuII CAG'CTG

Cuts at:      0    3059    3967    5632    6755    6798 
   Size:       3059     908    1665    1123      43

  Fragments arranged by size: 

                3059    1665    1123     908      43
 
SacI G_AGCT'C

Cuts at:      0     859    2282    6030    6798 
   Size:        859    1423    3748     768

  Fragments arranged by size: 

                3748    1423     859     768
 
SanDI GG'GwC_CC

Cuts at:      0    5558    6798 
   Size:       5558    1240
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2149    6798 
   Size:       2149    4649
 
ScaI AGT'ACT

Cuts at:      0     948    2364    3486    6798 
   Size:        948    1416    1122    3312

  Fragments arranged by size: 

                3312    1416    1122     948
 
SexAI A'CCwGG_T

Cuts at:      0     663    4310    6798 
   Size:        663    3647    2488

  Fragments arranged by size: 

                3647    2488     663
 
SfcI C'TryA_G

Cuts at:      0      76     181     253    2053    2078    2531    2699    2924 
   Size:         76     105      72    1800      25     453     168     225

Cuts at:   2924    3345    3416    4838    5102    5140    5588    5979    6042 
   Size:        421      71    1422     264      38     448     391      63

Cuts at:   6042    6798 
   Size:        756

  Fragments arranged by size: 

                1800    1422     756     453     448     421     391     264
                 225     168     105      76      72      71      63      38
                  25
 
SfoI GGC'GCC

Cuts at:      0      29    6798 
   Size:         29    6769
 
SmaI CCC'GGG

Cuts at:      0      33    1073    6798 
   Size:         33    1040    5725

  Fragments arranged by size: 

                5725    1040      33
 
SmlI C'TyrA_G

Cuts at:      0     910    1324    1514    1796    2115    2631    3535    6195 
   Size:        910     414     190     282     319     516     904    2660

Cuts at:   6195    6666    6798 
   Size:        471     132

  Fragments arranged by size: 

                2660     910     904     516     471     414     319     282
                 190     132
 
SphI G_CATG'C

Cuts at:      0    6607    6798 
   Size:       6607     191
 
SspI AAT'ATT

Cuts at:      0    5316    6573    6798 
   Size:       5316    1257     225

  Fragments arranged by size: 

                5316    1257     225
 
StuI AGG'CCT

Cuts at:      0    6724    6798 
   Size:       6724      74
 
StyI C'CwwG_G

Cuts at:      0     809    1602    1904    3971    4930    5047    6625    6798 
   Size:        809     793     302    2067     959     117    1578     173

  Fragments arranged by size: 

                2067    1578     959     809     793     302     173     117
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2207    2511    6798 
   Size:       2207     304    4287

  Fragments arranged by size: 

                4287    2207     304
 
TatI w'GTAC_w

Cuts at:      0     921     946    1482    2362    3484    6798 
   Size:        921      25     536     880    1122    3314

  Fragments arranged by size: 

                3314    1122     921     880     536      25
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1060    1092    3066    3098    5250    5282    5316    5348 
   Size:       1060      32    1974      32    2152      32      34      32

Cuts at:   5348    6798 
   Size:       1450

  Fragments arranged by size: 

                2152    1974    1450    1060      34      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1144    3027    4312    6798 
   Size:       1144    1883    1285    2486

  Fragments arranged by size: 

                2486    1883    1285    1144
 
XbaI T'CTAG_A

Cuts at:      0    1765    6798 
   Size:       1765    5033
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1942    6798 
   Size:       1942    4856
 
XhoI C'TCGA_G

Cuts at:      0    1324    1514    6798 
   Size:       1324     190    5284

  Fragments arranged by size: 

                5284    1324     190
 
XmaI C'CCGG_G

Cuts at:      0      31    1071    6798 
   Size:         31    1040    5727

  Fragments arranged by size: 

                5727    1040      31
 
XmnI GAAnn'nnTTC

Cuts at:      0     986    2606    5664    6062    6551    6798 
   Size:        986    1620    3058     398     489     247

  Fragments arranged by size: 

                3058    1620     986     489     398     247
 
ZraI GAC'GTC

Cuts at:      0    3458    6798 
   Size:       3458    3340
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI   AflIII     AgeI     AhdI
     AleI     AlfI    AlwNI     ApaI     ApoI     AseI     AvaI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI    BglII
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrFI
    BssSI   BstAPI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI     DraI   DraIII     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MmeI     MscI
     MslI   MspA1I     NarI     NcoI     NdeI     NruI     NsiI     NspI
     PasI     PciI     PfoI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PstI    PvuII     SacI    SanDI     SapI     ScaI    SexAI
     SfcI     SfoI     SmaI     SmlI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AclI     AfeI    AflII     AloI    ApaLI     AscI    AsiSI    AvrII
     BaeI    BamHI    BbvCI     BglI     BlpI     BmtI    BsaAI    BsiEI
    BsiWI    BsrBI    BsrGI   BssHII    BstBI   BstEII    CspCI     DrdI
     EagI     FseI     FspI    FspAI     MluI     NaeI   NgoMIV     NheI
     NotI     PacI    PflMI     PmeI     PmlI     PsrI     PvuI    RsrII
    SacII     SalI     SbfI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SrfI     SwaI
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