Restriction map for pF1KA0910
##################################
# NO CUT ENZYMES (   79 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AgeI        
AhdI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp718I     AspEI       AvrII       BalI        
BanI        BbeI        BclI        BglI        BlnI        
BsiWI       BsmBI       BspHI       BspLU11I    BsrBI       
BsrFI       BssHII      Bst1107I    BstZ17I     Cfr10I      
CpoI        DraIII      DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      FbaI        
FseI        HaeII       HincII      HindII      HpaI        
KasI        KpnI        KspI        MluI        MluNI       
MscI        NaeI        NarI        NcoI        NgoMIV      
NotI        NruI        PacI        PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SbfI        SfiI        
SfoI        SgrAI       SmaI        SnaBI       Sse8387I    
SwaI        XcmI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   65 enzymes) #
###################################

AccIII         20  TCCGGA               T^CCGGA
AcyI         1903  GGCGTC               G[GA]^CG[CT]C
AflII         865  CTTAAG               C^TTAAG
AflIII       2516  ACACGT               A^C[GA][CT]GT
Alw44I       3239  GTGCAC               G^TGCAC
AocI         3631  CCTCAGG              CC^T[ACGT]AGG
ApaLI        3239  GTGCAC               G^TGCAC
AvaI         1478  CTCGAG               C^[CT]CG[GA]G
AviII         432  TGCGCA               TGC^GCA
BamHI          39  GGATCC               G^GATCC
BbrPI        2517  CACGTG               CAC^GTG
BbvCI        3079  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1138  CGAACAAACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        3472  GGATAC               GTATCC(6/5)
BfrI          865  CTTAAG               C^TTAAG
BlpI         2554  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2554  GCTGAGC              GC^T[ACGT]AGC
BsaAI        2517  CACGTG               [CT]AC^GT[GA]
BsaHI        1903  GGCGTC               G[GA]^CG[CT]C
BsaWI          20  TCCGGA               [AT]^CCGG[AT]
BseAI          20  TCCGGA               T^CCGGA
BsiEI        3290  CGGTCG               CG[GA][CT]^CG
BsoBI        1478  CTCGAG               C^[CT]CG[GA]G
Bsp1407I     2513  TGTACA               T^GTACA
BspEI          20  TCCGGA               T^CCGGA
BsrGI        2513  TGTACA               T^GTACA
BssSI        3503  CTCGTG               CACGAG(-5/-1)
BstBI        1136  TTCGAA               TT^CGAA
BstEII       2331  GGTAACC              G^GT[ACGT]ACC
BstPI        2331  GGTAACC              G^GT[ACGT]ACC
Bsu36I       3631  CCTCAGG              CC^T[ACGT]AGG
CelII        2554  GCTGAGC              GC^T[ACGT]AGC
DraI         2901  TTTAAA               TTT^AAA
DrdI         1521  GACATTACAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       3631  CCTCAGG              CC^T[ACGT]AGG
EcoNI        3439  CCTCAAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      2331  GGTAACC              G^GT[ACGT]ACC
EcoRI         369  GAATTC               G^AATTC
EcoRV         979  GATATC               GAT^ATC
EcoT14I      3111  CCTTGG               C^C[AT][AT]GG
EcoT22I       173  ATGCAT               ATGCA^T
FspI          432  TGCGCA               TGC^GCA
Hin1I        1903  GGCGTC               G[GA]^CG[CT]C
MfeI         1757  CAATTG               C^AATTG
MroI           20  TCCGGA               T^CCGGA
MslI         2164  CATGACTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       2459  CAGCTG               C[AC]G^C[GT]G
MunI         1757  CAATTG               C^AATTG
NheI         1203  GCTAGC               G^CTAGC
NsiI          173  ATGCAT               ATGCA^T
NspV         1136  TTCGAA               TT^CGAA
PaeR7I       1478  CTCGAG               C^TCGAG
PflMI        2671  CCACCAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        2517  CACGTG               CAC^GTG
PmlI         2517  CACGTG               CAC^GTG
PvuII        2459  CAGCTG               CAG^CTG
SexAI         640  ACCAGGT              A^CC[AT]GGT
SfuI         1136  TTCGAA               TT^CGAA
SpeI         3142  ACTAGT               A^CTAGT
SspBI        2513  TGTACA               T^GTACA
SspI         1577  AATATT               AAT^ATT
StuI         2578  AGGCCT               AGG^CCT
StyI         3111  CCTTGG               C^C[AT][AT]GG
Van91I       2671  CCACCAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1478  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwNI        1322  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
             3461  CAGGACCTG           
AspI         2416  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3422  GACGCAGTC           
BanII        1331  GAGCTC               G[GA]GC[CT]^C
             2230  GAGCTC              
BglII        2466  AGATCT               A^GATCT
             2472  AGATCT              
BsaBI         334  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2467  GATCTAGATC          
BsaI         3324  GAGACC               GGTCTC(1/5)
             2691  GGTCTC              
BsmI         1785  GAATGC               GAATGC(1/-1)
             2024  GAATGC              
BspDI         322  ATCGAT               AT^CGAT
             2203  ATCGAT              
BsrDI        2961  CATTGC               GCAATG(2/0)
             2966  CATTGC              
BstXI         161  CCAAAGTACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              760  CCATTGTTCTGG        
ClaI          322  ATCGAT               AT^CGAT
             2203  ATCGAT              
DraII        3174  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             3462  AGGACCT             
EcoO109I     3174  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             3462  AGGACCT             
MamI          334  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2467  GATCTAGATC          
NdeI          176  CATATG               CA^TATG
              925  CATATG              
PflFI        2416  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3422  GACGCAGTC           
PpuMI        3174  GGGTCCT              [GA]G^G[AT]CC[CT]
             3462  AGGACCT             
SacI         1331  GAGCTC               GAGCT^C
             2230  GAGCTC              
ScaI          165  AGTACT               AGT^ACT
             1545  AGTACT              
SphI          443  GCATGC               GCATG^C
             1935  GCATGC              
Tth111I      2416  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3422  GACGCAGTC           
XbaI         1074  TCTAGA               T^CTAGA
             2469  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        Asp700I     AspHI       AvaII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       EarI        
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HindIII     
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        RsaI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmnI        
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