Restriction map for
pF1KA0910
(Circular) (Six-base) MAPSORT of: pF1KA0910.seq Check: 2481 from: 1 to: 6950
pF1KA0910 6950 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 954 954
Size: 6950
AccI GT'mk_AC
Cuts at: 3988 3988
Size: 6950
Acc65I G'GTAC_C
Cuts at: 3966 3966
Size: 6950
AclI AA'CG_TT
Cuts at: 6790 6790
Size: 6950
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 407 422 1936 2591 2689 2753 3188 3431 3754
Size: 15 1514 655 98 64 435 243 323
Cuts at: 3754 3887 4775 5207 5954 6222 407
Size: 133 888 432 747 268 1135
Fragments arranged by size:
1514 1135 888 747 655 435 432 323
268 243 133 98 64 15
AflII C'TTAA_G
Cuts at: 973 973
Size: 6950
AflIII A'CryG_T
Cuts at: 2624 4302 5406 6383 2624
Size: 1678 1104 977 3191
Fragments arranged by size:
3191 1678 1104 977
AgeI A'CCGG_T
Cuts at: 5338 5338
Size: 6950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2662 2888 3091 2662
Size: 226 203 6521
Fragments arranged by size:
6521 226 203
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1202 1234 1202
Size: 32 6918
AlwNI CAG_nnn'CTG
Cuts at: 1435 3574 4255 5822 1435
Size: 2139 681 1567 2563
Fragments arranged by size:
2563 2139 1567 681
ApaLI G'TGCA_C
Cuts at: 3347 5720 3347
Size: 2373 4577
ApoI r'AATT_y
Cuts at: 374 477 1005 1101 1674 1703 2615 3033 3481
Size: 103 528 96 573 29 912 418 448
Cuts at: 3481 3846 3954 6303 6559 374
Size: 365 108 2349 256 765
Fragments arranged by size:
2349 912 765 573 528 448 418 365
256 108 103 96 29
AseI AT'TA_AT
Cuts at: 20 20
Size: 6950
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6950
AvaI C'yCGr_G
Cuts at: 1586 3970 1586
Size: 2384 4566
BamHI G'GATC_C
Cuts at: 147 3975 147
Size: 3828 3122
BanI G'GyrC_C
Cuts at: 3966 4380 4628 4663 3966
Size: 414 248 35 6253
Fragments arranged by size:
6253 414 248 35
BanII G_rGCy'C
Cuts at: 1443 2342 3964 4994 1443
Size: 899 1622 1030 3399
Fragments arranged by size:
3399 1622 1030 899
BbeI G_GCGC'C
Cuts at: 4384 4632 4384
Size: 248 6702
BbsI GAAGACnn'nnnn_
Cuts at: 1963 2089 2677 3669 3755 1963
Size: 126 588 992 86 5158
Fragments arranged by size:
5158 992 588 126 86
BbvCI CC'TCA_GC
Cuts at: 3188 3188
Size: 6950
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1235 1269 1235
Size: 34 6916
BciVI GTATCCnnnnn_n'
Cuts at: 3574 4848 5609 6950 3574
Size: 1274 761 1341 3574
Fragments arranged by size:
3574 1341 1274 761
BclI T'GATC_A
Cuts at: 4470 4470
Size: 6950
BfrBI ATG'CAT
Cuts at: 283 283
Size: 6950
BglII A'GATC_T
Cuts at: 2574 2580 4465 6502 2574
Size: 6 1885 2037 3022
Fragments arranged by size:
3022 2037 1885 6
BlpI GC'TnA_GC
Cuts at: 2663 4062 2663
Size: 1399 5551
Bme1580I G_kGCm'C
Cuts at: 3351 3633 4575 4668 5724 3351
Size: 282 942 93 1056 4577
Fragments arranged by size:
4577 1056 942 282 93
BmrI ACTGGGnnnn_n'
Cuts at: 1558 3338 4162 4243 4578 6722 1558
Size: 1780 824 81 335 2144 1786
Fragments arranged by size:
2144 1786 1780 824 335 81
BmtI G_CTAG'C
Cuts at: 1315 1315
Size: 6950
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2961 3455 5317 2961
Size: 494 1862 4594
Fragments arranged by size:
4594 1862 494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1457 1950 2618 3181 3638 1457
Size: 493 668 563 457 4769
Fragments arranged by size:
4769 668 563 493 457
Bpu10I CC'TnA_GC
Cuts at: 3188 3188
Size: 6950
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1151 3533 4126 5005 5497 5795 1151
Size: 2382 593 879 492 298 2306
Fragments arranged by size:
2382 2306 879 593 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2805 3426 6654 2805
Size: 621 3228 3101
Fragments arranged by size:
3228 3101 621
BsaAI yAC'GTr
Cuts at: 2627 4303 2627
Size: 1676 5274
BsaBI GATnn'nnATC
Cuts at: 6 446 2579 4464 6501 6
Size: 440 2133 1885 2037 455
Fragments arranged by size:
2133 2037 1885 455 440
BsaHI Gr'CG_yC
Cuts at: 2012 4381 4629 5331 6827 2012
Size: 2369 248 702 1496 2135
Fragments arranged by size:
2369 2135 1496 702 248
BsaWI w'CCGG_w
Cuts at: 128 4139 4660 5338 5612 5759 6170 128
Size: 4011 521 678 274 147 411 908
Fragments arranged by size:
4011 908 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2433 2463 2616 2646 3591 3621 2433
Size: 30 153 30 945 30 5762
Fragments arranged by size:
5762 945 153 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 677 3428 3599 677
Size: 2751 171 4028
Fragments arranged by size:
4028 2751 171
BseYI C'CCAG_C
Cuts at: 742 3232 3839 4375 5133 5710 6206 742
Size: 2490 607 536 758 577 496 1486
Fragments arranged by size:
2490 1486 758 607 577 536 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1306 2632 3449 1306
Size: 1326 817 4807
Fragments arranged by size:
4807 1326 817
BsiEI CG_ry'CG
Cuts at: 104 3401 4492 4538 5746 104
Size: 3297 1091 46 1208 1308
Fragments arranged by size:
3297 1308 1208 1091 46
BsiHKAI G_wGCw'C
Cuts at: 230 1443 1476 1593 2342 3321 3351 3492 3964
Size: 1213 33 117 749 979 30 141 472
Cuts at: 3964 4742 4932 5724 230
Size: 778 190 792 1456
Fragments arranged by size:
1456 1213 979 792 778 749 472 190
141 117 33 30
BsmI GAATG_Cn'
Cuts at: 1899 2138 1899
Size: 239 6711
Bsp1286I G_dGCh'C
Cuts at: 230 1443 1476 1593 2342 3321 3351 3492 3633
Size: 1213 33 117 749 979 30 141 141
Cuts at: 3633 3964 4575 4668 4742 4932 4994 5724 230
Size: 331 611 93 74 190 62 730 1456
Fragments arranged by size:
1456 1213 979 749 730 611 331 190
141 141 117 93 74 62 33 30
BspEI T'CCGG_A
Cuts at: 128 6170 128
Size: 6042 908
BspHI T'CATG_A
Cuts at: 6126 6126
Size: 6950
BspMI ACCTGCnnnn'nnnn_
Cuts at: 954 3582 3872 4000 4214 4516 4897 954
Size: 2628 290 128 214 302 381 3007
Fragments arranged by size:
3007 2628 381 302 290 214 128
BsrBI CCG'CTC
Cuts at: 50 5242 5296 6780 6945 50
Size: 5192 54 1484 165 55
Fragments arranged by size:
5192 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3068 3073 4862 3068
Size: 5 1789 5156
Fragments arranged by size:
5156 1789 5
BsrFI r'CCGG_y
Cuts at: 4948 5129 5338 4948
Size: 181 209 6560
Fragments arranged by size:
6560 209 181
BsrGI T'GTAC_A
Cuts at: 2621 2621
Size: 6950
BssSI C'ACGA_G
Cuts at: 3611 5221 5579 3611
Size: 1610 358 4982
Fragments arranged by size:
4982 1610 358
BstAPI GCAn_nnn'nTGC
Cuts at: 505 3121 3559 4327 505
Size: 2616 438 768 3128
Fragments arranged by size:
3128 2616 768 438
BstBI TT'CG_AA
Cuts at: 1245 5311 1245
Size: 4066 2884
BstEII G'GTnAC_C
Cuts at: 2439 2439
Size: 6950
BstXI CCAn_nnnn'nTGG
Cuts at: 276 875 276
Size: 599 6351
BstYI r'GATC_y
Cuts at: 1 131 147 859 901 1015 2574 2580 3975
Size: 130 16 712 42 114 1559 6 1395
Cuts at: 3975 4442 4465 4800 5046 6058 6144 6156 6502
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6502 1
Size: 449
Fragments arranged by size:
1559 1395 1012 712 467 449 346 335
246 130 114 86 42 23 16 12
6
Bsu36I CC'TnA_GG
Cuts at: 3740 3740
Size: 6950
BtgI C'CryG_G
Cuts at: 107 107
Size: 6950
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4900 5081 5244 4900
Size: 181 163 6606
Fragments arranged by size:
6606 181 163
BtsI GCAGTG_nn'
Cuts at: 421 4193 421
Size: 3772 3178
ClaI AT'CG_AT
Cuts at: 431 2312 431
Size: 1881 5069
DraI TTT'AAA
Cuts at: 3011 3949 3011
Size: 938 6012
DrdI GACnn_nn'nnGTC
Cuts at: 1635 4656 5514 1635
Size: 3021 858 3071
Fragments arranged by size:
3071 3021 858
EaeI y'GGCC_r
Cuts at: 4535 4709 5100 5127 6472 4535
Size: 174 391 27 1345 5013
Fragments arranged by size:
5013 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4535 4535
Size: 6950
EarI CTCTTCn'nnn_
Cuts at: 2143 2652 2729 3243 4973 5183 2143
Size: 509 77 514 1730 210 3910
Fragments arranged by size:
3910 1730 514 509 210 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3431 5158 5468 5614 6760 3431
Size: 1727 310 146 1146 3621
Fragments arranged by size:
3621 1727 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1441 2340 3962 1441
Size: 899 1622 4429
Fragments arranged by size:
4429 1622 899
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 407 422 1457 1936 1950 2591 2618 2689 2753
Size: 15 1035 479 14 641 27 71 64
Cuts at: 2753 3181 3188 3431 3638 3754 3887 4775 5207
Size: 428 7 243 207 116 133 888 432
Cuts at: 5207 5954 6222 407
Size: 747 268 1135
Fragments arranged by size:
1135 1035 888 747 641 479 432 428
268 243 207 133 116 71 64 27
15 14 7
EcoNI CCTnn'n_nnAGG
Cuts at: 3551 3551
Size: 6950
EcoO109I rG'GnC_Cy
Cuts at: 3283 3571 4089 3283
Size: 288 518 6144
Fragments arranged by size:
6144 518 288
EcoRI G'AATT_C
Cuts at: 477 3954 477
Size: 3477 3473
EcoRV GAT'ATC
Cuts at: 1089 1089
Size: 6950
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1064 2460 2484 2911 3574 3817 4969 1064
Size: 1396 24 427 663 243 1152 3045
Fragments arranged by size:
3045 1396 1152 663 427 243 24
FspI TGC'GCA
Cuts at: 542 4731 542
Size: 4189 2761
FspAI rTGC'GCAy
Cuts at: 542 542
Size: 6950
HaeII r_GCGC'y
Cuts at: 4384 4632 5654 6633 4384
Size: 248 1022 979 4701
Fragments arranged by size:
4701 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 116 148 403 435 656 688 1700 1732 3591
Size: 32 255 32 221 32 1012 32 1859
Cuts at: 3591 3594 3623 3626 3922 3954 4792 4822 4824
Size: 3 29 3 296 32 838 30 2
Cuts at: 4824 4854 116
Size: 30 2212
Fragments arranged by size:
2212 1859 1012 838 296 255 221 32
32 32 32 32 30 30 29 3
3 2
HincII GTy'rAC
Cuts at: 3989 3989
Size: 6950
HindIII A'AGCT_T
Cuts at: 1046 1067 3908 1046
Size: 21 2841 4088
Fragments arranged by size:
4088 2841 21
KasI G'GCGC_C
Cuts at: 4380 4628 4380
Size: 248 6702
KpnI G_GTAC'C
Cuts at: 3970 3970
Size: 6950
MfeI C'AATT_G
Cuts at: 1865 1865
Size: 6950
MluI A'CGCG_T
Cuts at: 6383 6383
Size: 6950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 348 1304 1698 2476 5621 5805 348
Size: 956 394 778 3145 184 1493
Fragments arranged by size:
3145 1493 956 778 394 184
MscI TGG'CCA
Cuts at: 4711 4711
Size: 6950
MslI CAynn'nnrTG
Cuts at: 2276 4939 5066 2276
Size: 2663 127 4160
Fragments arranged by size:
4160 2663 127
MspA1I CmG'CkG
Cuts at: 2569 4061 4375 4735 5748 5993 2569
Size: 1492 314 360 1013 245 3526
Fragments arranged by size:
3526 1492 1013 360 314 245
NaeI GCC'GGC
Cuts at: 5131 5131
Size: 6950
NarI GG'CG_CC
Cuts at: 4381 4629 4381
Size: 248 6702
NcoI C'CATG_G
Cuts at: 107 107
Size: 6950
NdeI CA'TA_TG
Cuts at: 285 1034 285
Size: 749 6201
NgoMIV G'CCGG_C
Cuts at: 5129 5129
Size: 6950
NheI G'CTAG_C
Cuts at: 1311 1311
Size: 6950
NsiI A_TGCA'T
Cuts at: 285 285
Size: 6950
NspI r_CATG'y
Cuts at: 555 1081 1668 2047 4003 4503 5410 555
Size: 526 587 379 1956 500 907 2095
Fragments arranged by size:
2095 1956 907 587 526 500 379
PciI A'CATG_T
Cuts at: 5406 5406
Size: 6950
PflMI CCAn_nnn'nTGG
Cuts at: 2785 2785
Size: 6950
PfoI T'CCnGG_A
Cuts at: 1820 1820
Size: 6950
PmeI GTTT'AAAC
Cuts at: 3949 3949
Size: 6950
PmlI CAC'GTG
Cuts at: 2627 2627
Size: 6950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 848 880 1425 1457 6089 6121 848
Size: 32 545 32 4632 32 1677
Fragments arranged by size:
4632 1677 545 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3283 3571 3283
Size: 288 6662
PsiI TTA'TAA
Cuts at: 2753 2899 2753
Size: 146 6804
PspXI vC'TCGA_Gb
Cuts at: 1586 1586
Size: 6950
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2753 2785 2753
Size: 32 6918
PstI C_TGCA'G
Cuts at: 240 2184 2867 3997 4682 240
Size: 1944 683 1130 685 2508
Fragments arranged by size:
2508 1944 1130 685 683
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6950
PvuII CAG'CTG
Cuts at: 2569 4375 4735 2569
Size: 1806 360 4784
Fragments arranged by size:
4784 1806 360
RsrII CG'GwC_CG
Cuts at: 5145 5145
Size: 6950
SacI G_AGCT'C
Cuts at: 1443 2342 3964 1443
Size: 899 1622 4429
Fragments arranged by size:
4429 1622 899
SalI G'TCGA_C
Cuts at: 3987 3987
Size: 6950
SapI GCTCTTCn'nnn_
Cuts at: 2143 2652 3243 4973 5183 2143
Size: 509 591 1730 210 3910
Fragments arranged by size:
3910 1730 591 509 210
SbfI CC_TGCA'GG
Cuts at: 3997 3997
Size: 6950
ScaI AGT'ACT
Cuts at: 275 1655 6467 275
Size: 1380 4812 758
Fragments arranged by size:
4812 1380 758
SexAI A'CCwGG_T
Cuts at: 748 748
Size: 6950
SfcI C'TryA_G
Cuts at: 33 236 1943 2166 2180 2863 3159 3993 4678
Size: 203 1707 223 14 683 296 834 685
Cuts at: 4678 5671 5862 33
Size: 993 191 1121
Fragments arranged by size:
1707 1121 993 834 685 683 296 223
203 191 14
SfoI GGC'GCC
Cuts at: 4382 4630 4382
Size: 248 6702
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6950
SmaI CCC'GGG
Cuts at: 3972 3972
Size: 6950
SmlI C'TyrA_G
Cuts at: 973 1166 1586 3548 4105 5020 5512 5774 973
Size: 193 420 1962 557 915 492 262 2149
Fragments arranged by size:
2149 1962 915 557 492 420 262 193
SpeI A'CTAG_T
Cuts at: 67 3250 67
Size: 3183 3767
SphI G_CATG'C
Cuts at: 555 2047 4003 4503 555
Size: 1492 1956 500 3002
Fragments arranged by size:
3002 1956 1492 500
SspI AAT'ATT
Cuts at: 1687 1687
Size: 6950
StuI AGG'CCT
Cuts at: 2688 2688
Size: 6950
StyI C'CwwG_G
Cuts at: 107 3219 4084 4438 107
Size: 3112 865 354 2619
Fragments arranged by size:
3112 2619 865 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1373 1818 3483 3507 3612 4533 4666 5125 5334
Size: 445 1665 24 105 921 133 459 209
Cuts at: 5334 1373
Size: 2989
Fragments arranged by size:
2989 1665 921 459 445 209 133 105
24
TatI w'GTAC_w
Cuts at: 273 818 1653 2621 3586 6465 273
Size: 545 835 968 965 2879 758
Fragments arranged by size:
2879 968 965 835 758 545
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1892 1924 2511 2543 2616 2648 6467 6499 1892
Size: 32 587 32 73 32 3819 32 2343
Fragments arranged by size:
3819 2343 587 73 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2527 3533 4747 2527
Size: 1006 1214 4730
Fragments arranged by size:
4730 1214 1006
XbaI T'CTAG_A
Cuts at: 1182 2577 3981 1182
Size: 1395 1404 4151
Fragments arranged by size:
4151 1404 1395
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4445 4445
Size: 6950
XhoI C'TCGA_G
Cuts at: 1586 1586
Size: 6950
XmaI C'CCGG_G
Cuts at: 3970 3970
Size: 6950
XmnI GAAnn'nnTTC
Cuts at: 432 1379 1504 3182 432
Size: 947 125 1678 4200
Fragments arranged by size:
4200 1678 947 125
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
FspAI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NsiI NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI ApaI AscI AvrII BaeI
BglI BmgBI BsiWI BsmBI BssHII BstZ17I CspCI DraIII
FseI HpaI NotI NruI PacI PasI PshAI PspOMI
SacII SanDI SfiI SgrAI SnaBI SrfI SwaI ZraI