Restriction map for pF1KA0866
##################################
# NO CUT ENZYMES (   63 enzymes) #
##################################

Acc65I      AclI        AgeI        Aor51HI     AscI        
Asp718I     AviII       AvrII       BanI        BbeI        
BbrPI       BlnI        BsaBI       BsiWI       BspDI       
BssHII      Bst1107I    BstEII      BstPI       BstZ17I     
ClaI        CpoI        DraI        DraIII      DrdI        
Eco47III    EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HindIII     HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MunI        NarI        NheI        NruI        NsiI        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RsrII       SacII       
SbfI        ScaI        SfoI        SgrAI       SpeI        
Sse8387I    SspI        SwaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AatII         751  GACGTC               GACGT^C
AccIII       5390  TCCGGA               T^CCGGA
AflII        2623  CTTAAG               C^TTAAG
Alw44I       1659  GTGCAC               G^TGCAC
AocI          312  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1659  GTGCAC               G^TGCAC
AseI         1159  ATTAAT               AT^TAAT
AsnI         1159  ATTAAT               AT^TAAT
BamHI        1367  GGATCC               G^GATCC
BcgI          740  GCATCAACTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1304  GGATAC               GTATCC(6/5)
BfrI         2623  CTTAAG               C^TTAAG
BlpI         5304  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     5304  GCTCAGC              GC^T[ACGT]AGC
BsaAI         345  TACGTA               [CT]AC^GT[GA]
BseAI        5390  TCCGGA               T^CCGGA
BspEI        5390  TCCGGA               T^CCGGA
BspHI        2246  TCATGA               T^CATGA
BspLU11I      419  ACATGT               A^CATGT
BsrFI        2132  GCCGGC               [GA]^CCGG[CT]
BstBI        4726  TTCGAA               TT^CGAA
Bsu36I        312  CCTGAGG              CC^T[ACGT]AGG
CelII        5304  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       2132  GCCGGC               [GA]^CCGG[CT]
EagI         4235  CGGCCG               C^GGCCG
EclXI        4235  CGGCCG               C^GGCCG
Eco52I       4235  CGGCCG               C^GGCCG
Eco81I        312  CCTGAGG              CC^T[ACGT]AGG
MroI         5390  TCCGGA               T^CCGGA
NaeI         2132  GCCGGC               GCC^GGC
NdeI         1283  CATATG               CA^TATG
NgoMIV       2132  GCCGGC               G^CCGGC
NotI         4234  GCGGCCGC             GC^GGCCGC
NspV         4726  TTCGAA               TT^CGAA
PshBI        1159  ATTAAT               AT^TAAT
RcaI         2246  TCATGA               T^CATGA
SalI          415  GTCGAC               G^TCGAC
SfiI          585  GGCCGTGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI         4726  TTCGAA               TT^CGAA
SnaBI         345  TACGTA               TAC^GTA
SphI          491  GCATGC               GCATG^C
XbaI         5815  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccI          415  GTCGAC               GT^[AC][GT]AC
             4002  GTCTAC              
AcsI          730  AAATTC               [GA]^AATT[CT]
             4354  AAATTT              
AflIII        419  ACATGT               A^C[GA][CT]GT
             3998  ACGTGT              
AhdI         1102  GACCAGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3925  GACGTGGCGTC         
ApoI          730  AAATTC               [GA]^AATT[CT]
             4354  AAATTT              
AspEI        1102  GACCAGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3925  GACGTGGCGTC         
BglII        1391  AGATCT               A^GATCT
             5009  AGATCT              
BsgI         3781  GTGCAG               GTGCAG(16/14)
             3801  GTGCAG              
Bsp1407I      422  TGTACA               T^GTACA
             1967  TGTACA              
BsrDI        2102  GCAATG               GCAATG(2/0)
             5624  GCAATG              
BsrGI         422  TGTACA               T^GTACA
             1967  TGTACA              
Eam1105I     1102  GACCAGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3925  GACGTGGCGTC         
NcoI          995  CCATGG               C^CATGG
             5747  CCATGG              
SexAI        4292  ACCTGGT              A^CC[AT]GGT
             4544  ACCTGGT             
SmaI         3821  CCCGGG               CCC^GGG
             4745  CCCGGG              
SspBI         422  TGTACA               T^GTACA
             1967  TGTACA              
StuI         2234  AGGCCT               AGG^CCT
             4481  AGGCCT              
XmaCI        3821  CCCGGG               C^CCGGG
             4745  CCCGGG              
XmaI         3821  CCCGGG               C^CCGGG
             4745  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  134 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        Asp700I     AspHI       
AspI        AvaI        AvaII       BalI        BanII       
BbsI        BbvCI       BbvI        BclI        BcnI        
BfaI        BglI        BmyI        BpmI        BpuAI       
BsaHI       BsaI        BsaJI       BsaWI       BseRI       
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspMI       BsrBI       BsrI        BssKI       BssSI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
FbaI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PaeR7I      PflFI       
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        SacI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     Van91I      
XcmI        XhoI        XhoII       XmnI        
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