Restriction map for pF1KA0866
 (Circular) (Six-base) MAPSORT of: pF1KA0866.seq  Check: 7296  from: 1  to: 9029



pF1KA0866  9029 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    506    2634    3876    5667     506 
   Size:       2128    1242    1791    3868

  Fragments arranged by size: 

                3868    2128    1791    1242
 
AatII G_ACGT'C

Cuts at:    863     863 
   Size:       9029
 
AccI GT'mk_AC

Cuts at:    524    4111    6067     524 
   Size:       3587    1956    3486

  Fragments arranged by size: 

                3587    3486    1956
 
Acc65I G'GTAC_C

Cuts at:   6045    6045 
   Size:       9029
 
AclI AA'CG_TT

Cuts at:   8869    8869 
   Size:       9029
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   3457    3664    4366    4984    5659    5680    6854    7286    8033 
   Size:        207     702     618     675      21    1174     432     747

Cuts at:   8033    8301    3457 
   Size:        268    4185

  Fragments arranged by size: 

                4185    1174     747     702     675     618     432     268
                 207      21
 
AflII C'TTAA_G

Cuts at:   2731    2731 
   Size:       9029
 
AflIII A'CryG_T

Cuts at:    527    4106    6381    7485    8462     527 
   Size:       3579    2275    1104     977    1094

  Fragments arranged by size: 

                3579    2275    1104    1094     977
 
AgeI A'CCGG_T

Cuts at:   7417    7417 
   Size:       9029
 
AhdI GACnn_n'nnGTC

Cuts at:   1215    4038    1215 
   Size:       2823    6206
 
AleI CACnn'nnGTG

Cuts at:   1423    2626    1423 
   Size:       1203    7826
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2233    4271    5830    2233 
   Size:       2038    1559    5432

  Fragments arranged by size: 

                5432    2038    1559
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    805     837    1711    1743    3098    3130     805 
   Size:         32     874      32    1355      32    6704

  Fragments arranged by size: 

                6704    1355     874      32      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:    690    2544    2824    2865    2964    3012    3264    3792    3897 
   Size:       1854     280      41      99      48     252     528     105

Cuts at:   3897    4074    4191    4392    6334    7901     690 
   Size:        177     117     201    1942    1567    1818

  Fragments arranged by size: 

                1942    1854    1818    1567     528     280     252     201
                 177     117     105      99      48      41
 
ApaI G_GGCC'C

Cuts at:   3930    4581    4716    3930 
   Size:        651     135    8243

  Fragments arranged by size: 

                8243     651     135
 
ApaLI G'TGCA_C

Cuts at:   1767    7799    1767 
   Size:       6032    2997
 
ApoI r'AATT_y

Cuts at:    838    4462    6033    8382    8638     838 
   Size:       3624    1571    2349     256    1229

  Fragments arranged by size: 

                3624    2349    1571    1229     256
 
AseI AT'TA_AT

Cuts at:     20    1268      20 
   Size:       1248    7781
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       9029
 
AvaI C'yCGr_G

Cuts at:   3929    4574    4612    4853    5230    5476    5987    6049    3929 
   Size:        645      38     241     377     246     511      62    6909

  Fragments arranged by size: 

                6909     645     511     377     246     241      62      38
            
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    717     750     717 
   Size:         33    8996
 
BamHI G'GATC_C

Cuts at:   1475    6054    1475 
   Size:       4579    4450
 
BanI G'GyrC_C

Cuts at:   6045    6459    6707    6742    6045 
   Size:        414     248      35    8332

  Fragments arranged by size: 

                8332     414     248      35
 
BanII G_rGCy'C

Cuts at:   1670    2020    3350    3611    3731    3930    3941    4581    4716 
   Size:        350    1330     261     120     199      11     640     135

Cuts at:   4716    5214    5498    6043    7073    1670 
   Size:        498     284     545    1030    3626

  Fragments arranged by size: 

                3626    1330    1030     640     545     498     350     284
                 261     199     135     120      11
 
BbeI G_GCGC'C

Cuts at:   6463    6711    6463 
   Size:        248    8781
 
BbsI GAAGACnn'nnnn_

Cuts at:   1179    2259    2701    2918    3581    4655    4747    4931    1179 
   Size:       1080     442     217     663    1074      92     184    5277

  Fragments arranged by size: 

                5277    1080    1074     663     442     217     184      92
            
 
BbvCI CC'TCA_GC

Cuts at:   2825    3035    4529    2825 
   Size:        210    1494    7325

  Fragments arranged by size: 

                7325    1494     210
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    837     871     837 
   Size:         34    8995
 
BciVI GTATCCnnnnn_n'

Cuts at:   1406    6927    7688    9029    1406 
   Size:       5521     761    1341    1406

  Fragments arranged by size: 

                5521    1406    1341     761
 
BclI T'GATC_A

Cuts at:   2357    3081    4467    6549    2357 
   Size:        724    1386    2082    4837

  Fragments arranged by size: 

                4837    2082    1386     724
 
BglI GCCn_nnn'nGGC

Cuts at:    700    2246    2488    2518     700 
   Size:       1546     242      30    7211

  Fragments arranged by size: 

                7211    1546     242      30
 
BglII A'GATC_T

Cuts at:   1499    5117    6544    8581    1499 
   Size:       3618    1427    2037    1947

  Fragments arranged by size: 

                3618    2037    1947    1427
 
BlpI GC'TnA_GC

Cuts at:   5413    6141    5413 
   Size:        728    8301
 
Bme1580I G_kGCm'C

Cuts at:   1055    1771    3930    4581    4716    4947    5466    6654    6747 
   Size:        716    2159     651     135     231     519    1188      93

Cuts at:   6747    7803    1055 
   Size:       1056    2281

  Fragments arranged by size: 

                2281    2159    1188    1056     716     651     519     231
                 135      93
 
BmgBI CAC'GTC

Cuts at:    389    1971    4035    4674     389 
   Size:       1582    2064     639    4744

  Fragments arranged by size: 

                4744    2064    1582     639
 
BmrI ACTGGGnnnn_n'

Cuts at:    172     204    6241    6322    6657    8801     172 
   Size:         32    6037      81     335    2144     400

  Fragments arranged by size: 

                6037    2144     400     335      81      32
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1530    7396    1530 
   Size:       5866    3163
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    668     802     995    1609    1676    2215    2887    2920    3004 
   Size:        134     193     614      67     539     672      33      84

Cuts at:   3004    3076    3286    3307    3313    3460    3475    3571    3814 
   Size:         72     210      21       6     147      15      96     243

Cuts at:   3814    3898    4042    4150    4213    4717    4738    4780    5014 
   Size:         84     144     108      63     504      21      42     234

Cuts at:   5014    5091    5272    5329    5350    5362    5584    5785    5954 
   Size:         77     181      57      21      12     222     201     169

Cuts at:   5954     668 
   Size:       3743

  Fragments arranged by size: 

                3743     672     614     539     504     243     234     222
                 210     201     193     181     169     147     144     134
                 108      96      84      84      77      72      67      63
                  57      42      33      21      21      21      15      12
                   6
 
Bpu10I CC'TnA_GC

Cuts at:   2825    3035    3610    3683    4529    5890    2825 
   Size:        210     575      73     846    1361    5964

  Fragments arranged by size: 

                5964    1361     846     575     210      73
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1798    2572    3040    3139    3334    3763    4807    5737    5863 
   Size:        774     468      99     195     429    1044     930     126

Cuts at:   5863    6205    7084    7576    7874    1798 
   Size:        342     879     492     298    2953

  Fragments arranged by size: 

                2953    1044     930     879     774     492     468     429
                 342     298     195     126      99
 
BsaI GGTCTCn'nnnn_

Cuts at:    886    3527    4714    5899    8733     886 
   Size:       2641    1187    1185    2834    1182

  Fragments arranged by size: 

                2834    2641    1187    1185    1182
 
BsaAI yAC'GTr

Cuts at:    455    6382     455 
   Size:       5927    3102
 
BsaBI GATnn'nnATC

Cuts at:      6    6543    8580       6 
   Size:       6537    2037     455

  Fragments arranged by size: 

                6537    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    860    1216    2426    4039    4787    6460    6708    7410    8906 
   Size:        356    1210    1613     748    1673     248     702    1496

Cuts at:   8906     860 
   Size:        983

  Fragments arranged by size: 

                1673    1613    1496    1210     983     748     702     356
                 248
 
BsaWI w'CCGG_w

Cuts at:    593    2258    5498    6218    6739    7417    7691    7838    8249 
   Size:       1665    3240     720     521     678     274     147     411

Cuts at:   8249     593 
   Size:       1373

  Fragments arranged by size: 

                3240    1665    1373     720     678     521     411     274
                 147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    373     388     403     418     607     637     805     835    1750 
   Size:         15      15      15     189      30     168      30     915

Cuts at:   1750    1780    3098    3128    3886    3916     373 
   Size:         30    1318      30     758      30    5486

  Fragments arranged by size: 

                5486    1318     915     758     189     168      30      30
                  30      30      30      15      15      15
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    277     696     813    1141    1144    1292    1612    1926    2458 
   Size:        419     117     328       3     148     320     314     532

Cuts at:   2458    2461    2674    2677    2782    2842    2884    2917    2920 
   Size:          3     213       3     105      60      42      33       3

Cuts at:   2920    3001    3004    3136    3388    3391    3559    3739    4147 
   Size:         81       3     132     252       3     168     180     408

Cuts at:   4147    4150    4183    4348    4498    4747    4885    4893    5266 
   Size:          3      33     165     150     249     138       8     373

Cuts at:   5266    5347    5350    5359    5362    5602    5782    5791    5794 
   Size:         81       3       9       3     240     180       9       3

Cuts at:   5794    5986     277 
   Size:        192    3320

  Fragments arranged by size: 

                3320     532     419     408     373     328     320     314
                 252     249     240     213     192     180     180     168
                 165     150     148     138     132     117     105      81
                  81      60      42      33      33       9       9       8
                   3       3       3       3       3       3       3       3
                   3       3
 
BseYI C'CCAG_C

Cuts at:   1059    1849    2026    2088    2954    4052    4322    5324    5786 
   Size:        790     177      62     866    1098     270    1002     462

Cuts at:   5786    6454    7212    7789    8285    1059 
   Size:        668     758     577     496    1803

  Fragments arranged by size: 

                1803    1098    1002     866     790     758     668     577
                 496     462     270     177      62
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   3910    3930    3910 
   Size:         20    9009
 
BsiEI CG_ry'CG

Cuts at:    104    1681    4346    5379    6571    6617    7825     104 
   Size:       1577    2665    1033    1192      46    1208    1308

  Fragments arranged by size: 

                2665    1577    1308    1208    1192    1033      46
 
BsiHKAI G_wGCw'C

Cuts at:   1670    1771    2020    3350    3611    3731    3941    5498    6043 
   Size:        101     249    1330     261     120     210    1557     545

Cuts at:   6043    6821    7011    7803    1670 
   Size:        778     190     792    2896

  Fragments arranged by size: 

                2896    1557    1330     792     778     545     261     249
                 210     190     120     101
 
BsmI GAATG_Cn'

Cuts at:   1726    2228    2313    2348    1726 
   Size:        502      85      35    8407

  Fragments arranged by size: 

                8407     502      85      35
 
BsmBI CGTCTCn'nnnn_

Cuts at:    929    3658    4906    5984     929 
   Size:       2729    1248    1078    3974

  Fragments arranged by size: 

                3974    2729    1248    1078
 
Bsp1286I G_dGCh'C

Cuts at:   1055    1670    1771    2020    3350    3611    3731    3930    3941 
   Size:        615     101     249    1330     261     120     199      11

Cuts at:   3941    4581    4716    4947    5214    5466    5498    6043    6654 
   Size:        640     135     231     267     252      32     545     611

Cuts at:   6654    6747    6821    7011    7073    7803    1055 
   Size:         93      74     190      62     730    2281

  Fragments arranged by size: 

                2281    1330     730     640     615     611     545     267
                 261     252     249     231     199     190     135     120
                 101      93      74      62      32      11
 
BspEI T'CCGG_A

Cuts at:   5498    8249    5498 
   Size:       2751    6278
 
BspHI T'CATG_A

Cuts at:   2354    8205    2354 
   Size:       5851    3178
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    506    2634    2786    3876    5583    5667    6079    6293    6595 
   Size:       2128     152    1090    1707      84     412     214     302

Cuts at:   6595    6976     506 
   Size:        381    2559

  Fragments arranged by size: 

                2559    2128    1707    1090     412     381     302     214
                 152      84
 
BsrBI CCG'CTC

Cuts at:     50     429    2706    3498    3924    4140    4617    5481    7321 
   Size:        379    2277     792     426     216     477     864    1840

Cuts at:   7321    7375    8859    9024      50 
   Size:         54    1484     165      55

  Fragments arranged by size: 

                2277    1840    1484     864     792     477     426     379
                 216     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2217    5739    6941    2217 
   Size:       3522    1202    4305

  Fragments arranged by size: 

                4305    3522    1202
 
BsrFI r'CCGG_y

Cuts at:   2240    7027    7208    7417    2240 
   Size:       4787     181     209    3852

  Fragments arranged by size: 

                4787    3852     209     181
 
BsrGI T'GTAC_A

Cuts at:    530    2075     530 
   Size:       1545    7484
 
BssSI C'ACGA_G

Cuts at:    550    2155    3310    4432    4903    5512    7300    7658     550 
   Size:       1605    1155    1122     471     609    1788     358    1921

  Fragments arranged by size: 

                1921    1788    1605    1155    1122     609     471     358
            
 
BstAPI GCAn_nnn'nTGC

Cuts at:    555    3012    3897    6406     555 
   Size:       2457     885    2509    3178

  Fragments arranged by size: 

                3178    2509    2457     885
 
BstBI TT'CG_AA

Cuts at:   4835    7390    4835 
   Size:       2555    6474
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1849    1893    4023    4035    4266    5160    1849 
   Size:         44    2130      12     231     894    5718

  Fragments arranged by size: 

                5718    2130     894     231      44      12
 
BstYI r'GATC_y

Cuts at:      1    1288    1475    1499    2294    3065    3443    4842    5117 
   Size:       1287     187      24     795     771     378    1399     275

Cuts at:   5117    6054    6521    6544    6879    7125    8137    8223    8235 
   Size:        937     467      23     335     246    1012      86      12

Cuts at:   8235    8581       1 
   Size:        346     449

  Fragments arranged by size: 

                1399    1287    1012     937     795     771     467     449
                 378     346     335     275     246     187      86      24
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:    421     421 
   Size:       9029
 
BtgI C'CryG_G

Cuts at:    107     695    1094    1103    1739    4265    4778    5837    5855 
   Size:        588     399       9     636    2526     513    1059      18

Cuts at:   5855     107 
   Size:       3281

  Fragments arranged by size: 

                3281    2526    1059     636     588     513     399      18
                   9
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    561    2506    3929    5538    6979    7160    7323     561 
   Size:       1945    1423    1609    1441     181     163    2267

  Fragments arranged by size: 

                2267    1945    1609    1441    1423     181     163
 
BtsI GCAGTG_nn'

Cuts at:   2611    2635    6272    2611 
   Size:         24    3637    5368

  Fragments arranged by size: 

                5368    3637      24
 
DraI TTT'AAA

Cuts at:   6028    6028 
   Size:       9029
 
DrdI GACnn_nn'nnGTC

Cuts at:   6735    7593    6735 
   Size:        858    8171
 
EaeI y'GGCC_r

Cuts at:    692    1379    1952    2000    2510    2849    3362    3395    3815 
   Size:        687     573      48     510     339     513      33     420

Cuts at:   3815    4025    4343    5258    5426    5624    5699    6614    6788 
   Size:        210     318     915     168     198      75     915     174

Cuts at:   6788    7179    7206    8551     692 
   Size:        391      27    1345    1170

  Fragments arranged by size: 

                1345    1170     915     915     687     573     513     510
                 420     391     339     318     210     198     174     168
                  75      48      33      27
 
EagI C'GGCC_G

Cuts at:   4343    6614    4343 
   Size:       2271    6758
 
EarI CTCTTCn'nnn_

Cuts at:    472     538    1570    2155    3152    3239    3244    3656    4274 
   Size:         66    1032     585     997      87       5     412     618

Cuts at:   4274    4285    4503    4580    4748    4870    7052    7262     472 
   Size:         11     218      77     168     122    2182     210    2239

  Fragments arranged by size: 

                2239    2182    1032     997     618     585     412     218
                 210     168     122      87      77      66      11       5
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    394    2269    3949    7237    7547    7693    8839     394 
   Size:       1875    1680    3288     310     146    1146     584

  Fragments arranged by size: 

                3288    1875    1680    1146     584     310     146
 
EcoICRI GAG'CTC

Cuts at:   1668    2018    3348    3609    3729    3939    5496    6041    1668 
   Size:        350    1330     261     120     210    1557     545    4656

  Fragments arranged by size: 

                4656    1557    1330     545     350     261     210     120
            
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    668     802     995    1609    1676    2215    2887    2920    3004 
   Size:        134     193     614      67     539     672      33      84

Cuts at:   3004    3076    3286    3307    3313    3457    3460    3475    3571 
   Size:         72     210      21       6     144       3      15      96

Cuts at:   3571    3664    3814    3898    4042    4150    4213    4366    4717 
   Size:         93     150      84     144     108      63     153     351

Cuts at:   4717    4738    4780    4984    5014    5091    5272    5329    5350 
   Size:         21      42     204      30      77     181      57      21

Cuts at:   5350    5362    5584    5659    5680    5785    5954    6854    7286 
   Size:         12     222      75      21     105     169     900     432

Cuts at:   7286    8033    8301     668 
   Size:        747     268    1396

  Fragments arranged by size: 

                1396     900     747     672     614     539     432     351
                 268     222     210     204     193     181     169     153
                 150     144     144     134     108     105      96      93
                  84      84      77      75      72      67      63      57
                  42      33      30      21      21      21      21      15
                  12       6       3
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1591    1963    2443    2782    2902    3478    3541    3838    4585 
   Size:        372     480     339     120     576      63     297     747

Cuts at:   4585    5233    1591 
   Size:        648    5387

  Fragments arranged by size: 

                5387     747     648     576     480     372     339     297
                 120      63
 
EcoO109I rG'GnC_Cy

Cuts at:    219    3483    3555    3651    3831    4560    4578    4713    4980 
   Size:       3264      72      96     180     729      18     135     267

Cuts at:   4980    5190    6168     219 
   Size:        210     978    3080

  Fragments arranged by size: 

                3264    3080     978     729     267     210     180     135
                  96      72      18
 
EcoRI G'AATT_C

Cuts at:   6033    6033 
   Size:       9029
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    165     255    5685    7048     165 
   Size:         90    5430    1363    2146

  Fragments arranged by size: 

                5430    2146    1363      90
 
FspI TGC'GCA

Cuts at:   6810    6810 
   Size:       9029
 
HaeII r_GCGC'y

Cuts at:   1401    2033    4200    4822    5304    5563    6463    6711    7733 
   Size:        632    2167     622     482     259     900     248    1022

Cuts at:   7733    8712    1401 
   Size:        979    1718

  Fragments arranged by size: 

                2167    1718    1022     979     900     632     622     482
                 259     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    546     578     607     639     808     840    1123    1155    1201 
   Size:         32      29      32     169      32     283      32      46

Cuts at:   1201    1233    1280    1312    1484    1516    2149    2181    3475 
   Size:         32      47      32     172      32     633      32    1294

Cuts at:   3475    3507    3655    3687    3886    3918    4024    4056    5102 
   Size:         32     148      32     199      32     106      32    1046

Cuts at:   5102    5134    5767    5799    6871    6901    6903    6933     546 
   Size:         32     633      32    1072      30       2      30    2642

  Fragments arranged by size: 

                2642    1294    1072    1046     633     633     283     199
                 172     169     148     106      47      46      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      30      30      29       2
            
 
HincII GTy'rAC

Cuts at:    483     525    4809    6068     483 
   Size:         42    4284    1259    3444

  Fragments arranged by size: 

                4284    3444    1259      42
 
KasI G'GCGC_C

Cuts at:   6459    6707    6459 
   Size:        248    8781
 
KpnI G_GTAC'C

Cuts at:   6049    6049 
   Size:       9029
 
MluI A'CGCG_T

Cuts at:   8462    8462 
   Size:       9029
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1964    4253    4463    7700    7884    1964 
   Size:       2289     210    3237     184    3109

  Fragments arranged by size: 

                3237    3109    2289     210     184
 
MscI TGG'CCA

Cuts at:   1381    2002    2512    3364    3397    4027    5260    5428    6790 
   Size:        621     510     852      33     630    1233     168    1362

Cuts at:   6790    1381 
   Size:       3620

  Fragments arranged by size: 

                3620    1362    1233     852     630     621     510     168
                  33
 
MslI CAynn'nnrTG

Cuts at:   1423    2353    2557    2626    3988    4907    7018    7145    1423 
   Size:        930     204      69    1362     919    2111     127    3307

  Fragments arranged by size: 

                3307    2111    1362     930     919     204     127      69
            
 
MspA1I CmG'CkG

Cuts at:   1237    1409    1527    1902    2073    2088    2202    2544    2760 
   Size:        172     118     375     171      15     114     342     216

Cuts at:   2760    2793    2982    3360    4128    4161    4425    4749    4954 
   Size:         33     189     378     768      33     264     324     205

Cuts at:   4954    5203    5328    5763    5820    6140    6454    6814    7827 
   Size:        249     125     435      57     320     314     360    1013

Cuts at:   7827    8072    1237 
   Size:        245    2194

  Fragments arranged by size: 

                2194    1013     768     435     378     375     360     342
                 324     320     314     264     249     245     216     205
                 189     172     171     125     118     114      57      33
                  33      15
 
NaeI GCC'GGC

Cuts at:   2242    7210    2242 
   Size:       4968    4061
 
NarI GG'CG_CC

Cuts at:   6460    6708    6460 
   Size:        248    8781
 
NcoI C'CATG_G

Cuts at:    107    1103    5855     107 
   Size:        996    4752    3281

  Fragments arranged by size: 

                4752    3281     996
 
NdeI CA'TA_TG

Cuts at:   1392    1392 
   Size:       9029
 
NgoMIV G'CCGG_C

Cuts at:   2240    7208    2240 
   Size:       4968    4061
 
NotI GC'GGCC_GC

Cuts at:   4343    4343 
   Size:       9029
 
NspI r_CATG'y

Cuts at:    531     603    2055    4815    6082    6582    7489     531 
   Size:         72    1452    2760    1267     500     907    2071

  Fragments arranged by size: 

                2760    2071    1452    1267     907     500      72
 
PciI A'CATG_T

Cuts at:    527    7485     527 
   Size:       6958    2071
 
PflMI CCAn_nnn'nTGG

Cuts at:   1260    4695    5517    1260 
   Size:       3435     822    4772

  Fragments arranged by size: 

                4772    3435     822
 
PfoI T'CCnGG_A

Cuts at:   1085    1478    1586    2270    3446    3476    3689    4058    5288 
   Size:        393     108     684    1176      30     213     369    1230

Cuts at:   5288    1085 
   Size:       4826

  Fragments arranged by size: 

                4826    1230    1176     684     393     369     213     108
                  30
 
PmeI GTTT'AAAC

Cuts at:   6028    6028 
   Size:       9029
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    805     837    3098    3130    8168    8200     805 
   Size:         32    2261      32    5038      32    1634

  Fragments arranged by size: 

                5038    2261    1634      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    219    3483    3831    4980    5190     219 
   Size:       3264     348    1149     210    4058

  Fragments arranged by size: 

                4058    3264    1149     348     210
 
PspOMI G'GGCC_C

Cuts at:   3926    4577    4712    3926 
   Size:        651     135    8243

  Fragments arranged by size: 

                8243     651     135
 
PspXI vC'TCGA_Gb

Cuts at:   4612    5476    4612 
   Size:        864    8165
 
PstI C_TGCA'G

Cuts at:   1172    1559    2645    2693    2798    3386    3902    3965    4250 
   Size:        387    1086      48     105     588     516      63     285

Cuts at:   4250    4892    4959    5060    5678    5819    6076    6761    1172 
   Size:        642      67     101     618     141     257     685    3440

  Fragments arranged by size: 

                3440    1086     685     642     618     588     516     387
                 285     257     141     105     101      67      63      48
            
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       9029
 
PvuII CAG'CTG

Cuts at:   1237    1527    2073    2088    2202    2544    2760    2793    2982 
   Size:        290     546      15     114     342     216      33     189

Cuts at:   2982    3360    4128    4161    4749    4954    5328    5763    6454 
   Size:        378     768      33     588     205     374     435     691

Cuts at:   6454    6814    1237 
   Size:        360    3452

  Fragments arranged by size: 

                3452     768     691     588     546     435     378     374
                 360     342     290     216     205     189     114      33
                  33      15
 
RsrII CG'GwC_CG

Cuts at:   7224    7224 
   Size:       9029
 
SacI G_AGCT'C

Cuts at:   1670    2020    3350    3611    3731    3941    5498    6043    1670 
   Size:        350    1330     261     120     210    1557     545    4656

  Fragments arranged by size: 

                4656    1557    1330     545     350     261     210     120
            
 
SalI G'TCGA_C

Cuts at:    523    6066     523 
   Size:       5543    3486
 
SanDI GG'GwC_CC

Cuts at:    219     219 
   Size:       9029
 
SapI GCTCTTCn'nnn_

Cuts at:   1570    3244    4748    7052    7262    1570 
   Size:       1674    1504    2304     210    3337

  Fragments arranged by size: 

                3337    2304    1674    1504     210
 
SbfI CC_TGCA'GG

Cuts at:   6076    6076 
   Size:       9029
 
ScaI AGT'ACT

Cuts at:   8546    8546 
   Size:       9029
 
SexAI A'CCwGG_T

Cuts at:   4400    4652    4400 
   Size:        252    8777
 
SfcI C'TryA_G

Cuts at:     33    1168    1367    1555    1651    2641    2689    2782    2794 
   Size:       1135     199     188      96     990      48      93      12

Cuts at:   2794    2929    3382    3898    3961    4246    4888    4955    5056 
   Size:        135     453     516      63     285     642      67     101

Cuts at:   5056    5674    5815    6072    6757    7750    7941      33 
   Size:        618     141     257     685     993     191    1121

  Fragments arranged by size: 

                1135    1121     993     990     685     642     618     516
                 453     285     257     199     191     188     141     135
                 101      96      93      67      63      48      12
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    700     700 
   Size:       9029
 
SfoI GGC'GCC

Cuts at:   6461    6709    6461 
   Size:        248    8781
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       9029
 
SmaI CCC'GGG

Cuts at:   3931    4855    6051    3931 
   Size:        924    1196    6909

  Fragments arranged by size: 

                6909    1196     924
 
SmlI C'TyrA_G

Cuts at:   1813    2587    2731    3019    3118    3349    3742    4612    4822 
   Size:        774     144     288      99     231     393     870     210

Cuts at:   4822    5230    5476    5752    5878    5987    6184    7099    7591 
   Size:        408     246     276     126     109     197     915     492

Cuts at:   7591    7853    1813 
   Size:        262    2989

  Fragments arranged by size: 

                2989     915     870     774     492     408     393     288
                 276     262     246     231     210     197     144     126
                 109      99
 
SnaBI TAC'GTA

Cuts at:    455     455 
   Size:       9029
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       9029
 
SphI G_CATG'C

Cuts at:    603    6082    6582     603 
   Size:       5479     500    3050

  Fragments arranged by size: 

                5479    3050     500
 
SrfI GCCC'GGGC

Cuts at:   3931    3931 
   Size:       9029
 
StuI AGG'CCT

Cuts at:   2344    4591    2344 
   Size:       2247    6782
 
StyI C'CwwG_G

Cuts at:    107     362     980    1103    1382    2675    2699    3761    4028 
   Size:        255     618     123     279    1293      24    1062     267

Cuts at:   4028    4556    4592    4664    5855    6011    6163    6517     107 
   Size:        528      36      72    1191     156     152     354    2619

  Fragments arranged by size: 

                2619    1293    1191    1062     618     528     354     279
                 267     255     156     152     123      72      36      24
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    366    1152    1695    2779    4081    4336    6612    6745    7204 
   Size:        786     543    1084    1302     255    2276     133     459

Cuts at:   7204    7413     366 
   Size:        209    1982

  Fragments arranged by size: 

                2276    1982    1302    1084     786     543     459     255
                 209     133
 
TatI w'GTAC_w

Cuts at:    530    2075    5708    8544     530 
   Size:       1545    3633    2836    1015

  Fragments arranged by size: 

                3633    2836    1545    1015
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    373     405    1256    1288    1750    1782    8546    8578     373 
   Size:         32     851      32     462      32    6764      32     824

  Fragments arranged by size: 

                6764     851     824     462      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   3669    3949    4654    5380    6826    3669 
   Size:        280     705     726    1446    5872

  Fragments arranged by size: 

                5872    1446     726     705     280
 
XbaI T'CTAG_A

Cuts at:   5923    6060    5923 
   Size:        137    8892
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1110    1149    1584    2325    3852    4188    4671    4713    4797 
   Size:         39     435     741    1527     336     483      42      84

Cuts at:   4797    5559    6524    1110 
   Size:        762     965    3615

  Fragments arranged by size: 

                3615    1527     965     762     741     483     435     336
                  84      42      39
 
XhoI C'TCGA_G

Cuts at:   4612    5230    5476    5987    4612 
   Size:        618     246     511    7654

  Fragments arranged by size: 

                7654     618     511     246
 
XmaI C'CCGG_G

Cuts at:   3929    4853    6049    3929 
   Size:        924    1196    6909

  Fragments arranged by size: 

                6909    1196     924
 
XmnI GAAnn'nnTTC

Cuts at:   1516    2228    4294    1516 
   Size:        712    2066    6251

  Fragments arranged by size: 

                6251    2066     712
 
ZraI GAC'GTC

Cuts at:    861     861 
   Size:       9029
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NotI     NspI     PciI    PflMI     PfoI     PmeI
     PpiI    PpuMI   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII
     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AscI    AvrII    BfrBI     BmtI    BsiWI   BssHII   BstEII
  BstZ17I     ClaI    CspCI   DraIII    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NheI     NruI     NsiI     PacI     PasI     PmlI
    PshAI     PsiI     PsrI    SacII    SgrAI     SspI     SwaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com