Restriction map for pF1KA0852
 (Linear) (Six-base) MAPSORT of: KIAA0852.seq  Check: 3665  from: 1  to: 2910



KIAA0852.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1136    2910 
   Size:       1136    1774
 
AatII G_ACGT'C

Cuts at:      0     499    2910 
   Size:        499    2411
 
AccI GT'mk_AC

Cuts at:      0      94    1638    2910 
   Size:         94    1544    1272

  Fragments arranged by size: 

                1544    1272      94
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     740    1531    1603    2674    2910 
   Size:        740     791      72    1071     236

  Fragments arranged by size: 

                1071     791     740     236      72
 
AflIII A'CryG_T

Cuts at:      0     650    2910 
   Size:        650    2260
 
AgeI A'CCGG_T

Cuts at:      0    1415    2910 
   Size:       1415    1495
 
AleI CACnn'nnGTG

Cuts at:      0    2254    2910 
   Size:       2254     656
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2633    2910 
   Size:       2633     277
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     943     975    2910 
   Size:        943      32    1935

  Fragments arranged by size: 

                1935     943      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1896    2041    2802    2910 
   Size:       1896     145     761     108

  Fragments arranged by size: 

                1896     761     145     108
 
ApaI G_GGCC'C

Cuts at:      0    1009    2437    2910 
   Size:       1009    1428     473

  Fragments arranged by size: 

                1428    1009     473
 
ApoI r'AATT_y

Cuts at:      0     304     912    1514    2625    2910 
   Size:        304     608     602    1111     285

  Fragments arranged by size: 

                1111     608     602     304     285
 
AvaI C'yCGr_G

Cuts at:      0     275    1787    1931    2726    2910 
   Size:        275    1512     144     795     184

  Fragments arranged by size: 

                1512     795     275     184     144
 
AvrII C'CTAG_G

Cuts at:      0     771    2910 
   Size:        771    2139
 
BamHI G'GATC_C

Cuts at:      0      33    2910 
   Size:         33    2877
 
BanI G'GyrC_C

Cuts at:      0    1124    1830    2910 
   Size:       1124     706    1080

  Fragments arranged by size: 

                1124    1080     706
 
BanII G_rGCy'C

Cuts at:      0     136    1009    1930    2141    2187    2437    2471    2687 
   Size:        136     873     921     211      46     250      34     216

Cuts at:   2687    2910 
   Size:        223

  Fragments arranged by size: 

                 921     873     250     223     216     211     136      46
                  34
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     185    1483    1984    2910 
   Size:        185    1298     501     926

  Fragments arranged by size: 

                1298     926     501     185
 
BbvCI CC'TCA_GC

Cuts at:      0    1643    2803    2910 
   Size:       1643    1160     107

  Fragments arranged by size: 

                1643    1160     107
 
BclI T'GATC_A

Cuts at:      0     410     983    1676    2910 
   Size:        410     573     693    1234

  Fragments arranged by size: 

                1234     693     573     410
 
BglI GCCn_nnn'nGGC

Cuts at:      0     522    2449    2910 
   Size:        522    1927     461

  Fragments arranged by size: 

                1927     522     461
 
Bme1580I G_kGCm'C

Cuts at:      0     511     593    1009    1064    2309    2437    2910 
   Size:        511      82     416      55    1245     128     473

  Fragments arranged by size: 

                1245     511     473     416     128      82      55
 
BmgBI CAC'GTC

Cuts at:      0     191     653    2910 
   Size:        191     462    2257

  Fragments arranged by size: 

                2257     462     191
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     366    2910 
   Size:        366    2544
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     543     767    2910 
   Size:        543     224    2143

  Fragments arranged by size: 

                2143     543     224
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     780    1096    1558    1801    1949    2910 
   Size:        780     316     462     243     148     961

  Fragments arranged by size: 

                 961     780     462     316     243     148
 
Bpu10I CC'TnA_GC

Cuts at:      0    1643    2515    2803    2910 
   Size:       1643     872     288     107

  Fragments arranged by size: 

                1643     872     288     107
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2161    2910 
   Size:       2161     749
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     773    2321    2524    2910 
   Size:        773    1548     203     386

  Fragments arranged by size: 

                1548     773     386     203
 
BsaAI yAC'GTr

Cuts at:      0     705    2208    2304    2910 
   Size:        705    1503      96     606

  Fragments arranged by size: 

                1503     705     606      96
 
BsaBI GATnn'nnATC

Cuts at:      0     574     580    2910 
   Size:        574       6    2330

  Fragments arranged by size: 

                2330     574       6
 
BsaHI Gr'CG_yC

Cuts at:      0     496    2876    2910 
   Size:        496    2380      34

  Fragments arranged by size: 

                2380     496      34
 
BsaWI w'CCGG_w

Cuts at:      0    1415    2382    2558    2910 
   Size:       1415     967     176     352

  Fragments arranged by size: 

                1415     967     352     176
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     370    1807    2074    2088    2437    2440    2800    2910 
   Size:        370    1437     267      14     349       3     360     110

  Fragments arranged by size: 

                1437     370     360     349     267     110      14       3
            
 
BseYI C'CCAG_C

Cuts at:      0    1690    2152    2333    2444    2910 
   Size:       1690     462     181     111     466

  Fragments arranged by size: 

                1690     466     462     181     111
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     619    1909    2188    2857    2910 
   Size:        619    1290     279     669      53

  Fragments arranged by size: 

                1290     669     619     279      53
 
BsiEI CG_ry'CG

Cuts at:      0    1657    2910 
   Size:       1657    1253
 
BsiHKAI G_wGCw'C

Cuts at:      0     554     704     891    1151    2141    2187    2521    2910 
   Size:        554     150     187     260     990      46     334     389

  Fragments arranged by size: 

                 990     554     389     334     260     187     150      46
            
 
BsmI GAATG_Cn'

Cuts at:      0     270    2488    2910 
   Size:        270    2218     422

  Fragments arranged by size: 

                2218     422     270
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     487    2773    2910 
   Size:        487    2286     137

  Fragments arranged by size: 

                2286     487     137
 
Bsp1286I G_dGCh'C

Cuts at:      0     136     511     554     593     704     891    1009    1064 
   Size:        136     375      43      39     111     187     118      55

Cuts at:   1064    1151    1930    2141    2187    2309    2437    2471    2521 
   Size:         87     779     211      46     122     128      34      50

Cuts at:   2521    2687    2910 
   Size:        166     223

  Fragments arranged by size: 

                 779     375     223     211     187     166     136     128
                 122     118     111      87      55      50      46      43
                  39      34
 
BspEI T'CCGG_A

Cuts at:      0    2382    2558    2910 
   Size:       2382     176     352

  Fragments arranged by size: 

                2382     352     176
 
BspHI T'CATG_A

Cuts at:      0     216    2910 
   Size:        216    2694
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1136    1677    1884    2549    2910 
   Size:       1136     541     207     665     361

  Fragments arranged by size: 

                1136     665     541     361     207
 
BsrBI CCG'CTC

Cuts at:      0     522    1568    2910 
   Size:        522    1046    1342

  Fragments arranged by size: 

                1342    1046     522
 
BsrDI GCAATG_nn'

Cuts at:      0    1145    2910 
   Size:       1145    1765
 
BsrFI r'CCGG_y

Cuts at:      0    1121    1415    2910 
   Size:       1121     294    1495

  Fragments arranged by size: 

                1495    1121     294
 
BsrGI T'GTAC_A

Cuts at:      0     626     641    2910 
   Size:        626      15    2269

  Fragments arranged by size: 

                2269     626      15
 
BssSI C'ACGA_G

Cuts at:      0    2527    2910 
   Size:       2527     383
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     597     715    2277    2835    2910 
   Size:        597     118    1562     558      75

  Fragments arranged by size: 

                1562     597     558     118      75
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2817    2910 
   Size:       2817      93
 
BstYI r'GATC_y

Cuts at:      0      33     575     945    2552    2910 
   Size:         33     542     370    1607     358

  Fragments arranged by size: 

                1607     542     370     358      33
 
Bsu36I CC'TnA_GG

Cuts at:      0     902    1826    2910 
   Size:        902     924    1084

  Fragments arranged by size: 

                1084     924     902
 
BtgI C'CryG_G

Cuts at:      0     585    2255    2910 
   Size:        585    1670     655

  Fragments arranged by size: 

                1670     655     585
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1164    2910 
   Size:       1164    1746
 
BtsI GCAGTG_nn'

Cuts at:      0     250    2355    2507    2910 
   Size:        250    2105     152     403

  Fragments arranged by size: 

                2105     403     250     152
 
DraIII CAC_nnn'GTG

Cuts at:      0     597    2910 
   Size:        597    2313
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1646    2545    2910 
   Size:       1646     899     365

  Fragments arranged by size: 

                1646     899     365
 
EaeI y'GGCC_r

Cuts at:      0      74    2910 
   Size:         74    2836
 
EarI CTCTTCn'nnn_

Cuts at:      0     202     716     856    1481    2153    2910 
   Size:        202     514     140     625     672     757

  Fragments arranged by size: 

                 757     672     625     514     202     140
 
EcoICRI GAG'CTC

Cuts at:      0    2139    2185    2910 
   Size:       2139      46     725

  Fragments arranged by size: 

                2139     725      46
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     740     780    1096    1531    1558    1603    1801    1949 
   Size:        740      40     316     435      27      45     198     148

Cuts at:   1949    2674    2910 
   Size:        725     236

  Fragments arranged by size: 

                 740     725     435     316     236     198     148      45
                  40      27
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2763    2830    2910 
   Size:       2763      67      80

  Fragments arranged by size: 

                2763      80      67
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1542    2434    2889    2910 
   Size:       1542     892     455      21

  Fragments arranged by size: 

                1542     892     455      21
 
EcoRI G'AATT_C

Cuts at:      0    2625    2910 
   Size:       2625     285
 
EcoRV GAT'ATC

Cuts at:      0     480    2910 
   Size:        480    2430
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2679    2910 
   Size:       2679     231
 
FspI TGC'GCA

Cuts at:      0     142     841    2755    2910 
   Size:        142     699    1914     155

  Fragments arranged by size: 

                1914     699     155     142
 
FspAI rTGC'GCAy

Cuts at:      0     142    2910 
   Size:        142    2768
 
HaeII r_GCGC'y

Cuts at:      0     528    2910 
   Size:        528    2382
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     448     480    2508    2540    2626    2658    2910 
   Size:        448      32    2028      32      86      32     252

  Fragments arranged by size: 

                2028     448     252      86      32      32      32
 
HincII GTy'rAC

Cuts at:      0     930    2910 
   Size:        930    1980
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1999    2910 
   Size:       1999     911
 
MslI CAynn'nnrTG

Cuts at:      0    2254    2910 
   Size:       2254     656
 
MspA1I CmG'CkG

Cuts at:      0     398     547    1014    1347    2333    2613    2910 
   Size:        398     149     467     333     986     280     297

  Fragments arranged by size: 

                 986     467     398     333     297     280     149
 
NcoI C'CATG_G

Cuts at:      0     585    2910 
   Size:        585    2325
 
NspI r_CATG'y

Cuts at:      0     960    1034    2275    2910 
   Size:        960      74    1241     635

  Fragments arranged by size: 

                1241     960     635      74
 
PasI CC'CwG_GG

Cuts at:      0    1452    2910 
   Size:       1452    1458
 
PfoI T'CCnGG_A

Cuts at:      0    1073    2910 
   Size:       1073    1837
 
PmlI CAC'GTG

Cuts at:      0    2208    2910 
   Size:       2208     702
 
PpuMI rG'GwC_Cy

Cuts at:      0    2889    2910 
   Size:       2889      21
 
PspOMI G'GGCC_C

Cuts at:      0    1005    2433    2910 
   Size:       1005    1428     477

  Fragments arranged by size: 

                1428    1005     477
 
PstI C_TGCA'G

Cuts at:      0    2798    2910 
   Size:       2798     112
 
PvuII CAG'CTG

Cuts at:      0    1014    1347    2613    2910 
   Size:       1014     333    1266     297

  Fragments arranged by size: 

                1266    1014     333     297
 
SacI G_AGCT'C

Cuts at:      0    2141    2187    2910 
   Size:       2141      46     723

  Fragments arranged by size: 

                2141     723      46
 
ScaI AGT'ACT

Cuts at:      0     337    2662    2910 
   Size:        337    2325     248

  Fragments arranged by size: 

                2325     337     248
 
SexAI A'CCwGG_T

Cuts at:      0    1067    2910 
   Size:       1067    1843
 
SfcI C'TryA_G

Cuts at:      0     972    2794    2910 
   Size:        972    1822     116

  Fragments arranged by size: 

                1822     972     116
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2449    2910 
   Size:       2449     461
 
SmaI CCC'GGG

Cuts at:      0     277    1789    2728    2910 
   Size:        277    1512     939     182

  Fragments arranged by size: 

                1512     939     277     182
 
SmlI C'TyrA_G

Cuts at:      0    2176    2910 
   Size:       2176     734
 
StuI AGG'CCT

Cuts at:      0    2737    2910 
   Size:       2737     173
 
StyI C'CwwG_G

Cuts at:      0     585     771    1112    1595    2732    2900    2910 
   Size:        585     186     341     483    1137     168      10

  Fragments arranged by size: 

                1137     585     483     341     186     168      10
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1575    1643    2004    2325    2910 
   Size:       1575      68     361     321     585

  Fragments arranged by size: 

                1575     585     361     321      68
 
TatI w'GTAC_w

Cuts at:      0     335     626     641     647    2660    2910 
   Size:        335     291      15       6    2013     250

  Fragments arranged by size: 

                2013     335     291     250      15       6
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     392     424    2013    2045    2910 
   Size:        392      32    1589      32     865

  Fragments arranged by size: 

                1589     865     392      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1983    2910 
   Size:       1983     927
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     257    2330    2910 
   Size:        257    2073     580

  Fragments arranged by size: 

                2073     580     257
 
XmaI C'CCGG_G

Cuts at:      0     275    1787    2726    2910 
   Size:        275    1512     939     184

  Fragments arranged by size: 

                1512     939     275     184
 
ZraI GAC'GTC

Cuts at:      0     497    2910 
   Size:        497    2413
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AgeI     AleI     AlfI
     AloI    AlwNI     ApaI     ApoI     AvaI    AvrII    BamHI     BanI
    BanII     BbsI    BbvCI     BclI     BglI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
   DraIII     DrdI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FspI    FspAI    HaeII    Hin4I   HincII
     MmeI     MslI   MspA1I     NcoI     NspI     PasI     PfoI     PmlI
    PpuMI   PspOMI     PstI    PvuII     SacI     ScaI    SexAI     SfcI
     SfiI     SmaI     SmlI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AhdI    ApaLI     AscI     AseI
    AsiSI     BaeI     BbeI     BcgI    BciVI    BfrBI    BglII     BlpI
     BmtI    BsaXI    BsiWI   BssHII    BstBI   BstEII  BstZ17I     ClaI
    CspCI     DraI     EagI     EciI     FseI  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI     PmeI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     SfoI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     XbaI     XhoI     XmnI
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