Restriction map for pF1KA0837
 (Linear) (Six-base) MAPSORT of: KIAA0837.seq  Check: 5865  from: 1  to: 2166



KIAA0837.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:21  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1191    1910    1984    2166 
   Size:       1191     719      74     182

  Fragments arranged by size: 

                1191     719     182      74
 
AfeI AGC'GCT

Cuts at:      0    1234    2166 
   Size:       1234     932
 
AflIII A'CryG_T

Cuts at:      0    1046    2057    2166 
   Size:       1046    1011     109

  Fragments arranged by size: 

                1046    1011     109
 
AleI CACnn'nnGTG

Cuts at:      0     163    2166 
   Size:        163    2003
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1894    1962    2166 
   Size:       1894      68     204

  Fragments arranged by size: 

                1894     204      68
 
AlwNI CAG_nnn'CTG

Cuts at:      0     435    2166 
   Size:        435    1731
 
ApaI G_GGCC'C

Cuts at:      0     395     622    1911    2166 
   Size:        395     227    1289     255

  Fragments arranged by size: 

                1289     395     255     227
 
ApaLI G'TGCA_C

Cuts at:      0    1461    2166 
   Size:       1461     705
 
ApoI r'AATT_y

Cuts at:      0     466    2166 
   Size:        466    1700
 
AvaI C'yCGr_G

Cuts at:      0    1783    2166 
   Size:       1783     383
 
BanI G'GyrC_C

Cuts at:      0     175    1427    1518    2166 
   Size:        175    1252      91     648

  Fragments arranged by size: 

                1252     648     175      91
 
BanII G_rGCy'C

Cuts at:      0      31     395     622    1911    2166 
   Size:         31     364     227    1289     255

  Fragments arranged by size: 

                1289     364     255     227      31
 
BbeI G_GCGC'C

Cuts at:      0    1522    2166 
   Size:       1522     644
 
BbvCI CC'TCA_GC

Cuts at:      0     311    2166 
   Size:        311    1855
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      92    2166 
   Size:         92    2074
 
BclI T'GATC_A

Cuts at:      0     513     743    2166 
   Size:        513     230    1423

  Fragments arranged by size: 

                1423     513     230
 
BglI GCCn_nnn'nGGC

Cuts at:      0     460    2166 
   Size:        460    1706
 
BglII A'GATC_T

Cuts at:      0    1202    1941    2166 
   Size:       1202     739     225

  Fragments arranged by size: 

                1202     739     225
 
BlpI GC'TnA_GC

Cuts at:      0     373    2166 
   Size:        373    1793
 
Bme1580I G_kGCm'C

Cuts at:      0     180     285     395     622     866    1465    1511    1911 
   Size:        180     105     110     227     244     599      46     400

Cuts at:   1911    2166 
   Size:        255

  Fragments arranged by size: 

                 599     400     255     244     227     180     110     105
                  46
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1581    2166 
   Size:       1581     585
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     472     713    1264    1394    1709    2166 
   Size:         88     384     241     551     130     315     457

  Fragments arranged by size: 

                 551     457     384     315     241     130      88
 
Bpu10I CC'TnA_GC

Cuts at:      0     113     311     416    1763    2111    2166 
   Size:        113     198     105    1347     348      55

  Fragments arranged by size: 

                1347     348     198     113     105      55
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     721    2166 
   Size:        721    1445
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2100    2166 
   Size:       2100      66
 
BsaAI yAC'GTr

Cuts at:      0    1512    2166 
   Size:       1512     654
 
BsaHI Gr'CG_yC

Cuts at:      0    1519    2166 
   Size:       1519     647
 
BsaWI w'CCGG_w

Cuts at:      0     478    1187    1277    1808    2166 
   Size:        478     709      90     531     358

  Fragments arranged by size: 

                 709     531     478     358      90
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1079    1109    1760    1790    2143    2166 
   Size:       1079      30     651      30     353      23

  Fragments arranged by size: 

                1079     651     353      30      30      23
 
BseYI C'CCAG_C

Cuts at:      0    1383    2166 
   Size:       1383     783
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1932    2166 
   Size:       1932     234
 
BsiHKAI G_wGCw'C

Cuts at:      0    1022    1465    2166 
   Size:       1022     443     701

  Fragments arranged by size: 

                1022     701     443
 
Bsp1286I G_dGCh'C

Cuts at:      0      31     180     285     395     622     866    1022    1465 
   Size:         31     149     105     110     227     244     156     443

Cuts at:   1465    1511    1911    2166 
   Size:         46     400     255

  Fragments arranged by size: 

                 443     400     255     244     227     156     149     110
                 105      46      31
 
BspEI T'CCGG_A

Cuts at:      0     478    1277    1808    2166 
   Size:        478     799     531     358

  Fragments arranged by size: 

                 799     531     478     358
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2019    2166 
   Size:       2019     147
 
BsrBI CCG'CTC

Cuts at:      0     603    2166 
   Size:        603    1563
 
BsrFI r'CCGG_y

Cuts at:      0     212    1710    2166 
   Size:        212    1498     456

  Fragments arranged by size: 

                1498     456     212
 
BssSI C'ACGA_G

Cuts at:      0      16     163    2166 
   Size:         16     147    2003

  Fragments arranged by size: 

                2003     147      16
 
BstBI TT'CG_AA

Cuts at:      0     764    2166 
   Size:        764    1402
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     594    2166 
   Size:        594    1572
 
BstYI r'GATC_y

Cuts at:      0      89    1202    1638    1941    1959    2166 
   Size:         89    1113     436     303      18     207

  Fragments arranged by size: 

                1113     436     303     207      89      18
 
Bsu36I CC'TnA_GG

Cuts at:      0      94    1503    2166 
   Size:         94    1409     663

  Fragments arranged by size: 

                1409     663      94
 
BtgI C'CryG_G

Cuts at:      0     366     587     824     942    1083    1839    2166 
   Size:        366     221     237     118     141     756     327

  Fragments arranged by size: 

                 756     366     327     237     221     141     118
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     946    1685    2166 
   Size:        946     739     481

  Fragments arranged by size: 

                 946     739     481
 
DraI TTT'AAA

Cuts at:      0    1755    2166 
   Size:       1755     411
 
EaeI y'GGCC_r

Cuts at:      0     152     214     452     544     837    1449    2166 
   Size:        152      62     238      92     293     612     717

  Fragments arranged by size: 

                 717     612     293     238     152      92      62
 
EarI CTCTTCn'nnn_

Cuts at:      0    1324    1909    2138    2166 
   Size:       1324     585     229      28

  Fragments arranged by size: 

                1324     585     229      28
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     352    1108    2166 
   Size:        352     756    1058

  Fragments arranged by size: 

                1058     756     352
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     472     713    1191    1264    1394    1709    1910 
   Size:         88     384     241     478      73     130     315     201

Cuts at:   1910    1984    2166 
   Size:         74     182

  Fragments arranged by size: 

                 478     384     315     241     201     182     130      88
                  74      73
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     116    2166 
   Size:        116    2050
 
EcoO109I rG'GnC_Cy

Cuts at:      0     392     619    1659    2166 
   Size:        392     227    1040     507

  Fragments arranged by size: 

                1040     507     392     227
 
EcoRV GAT'ATC

Cuts at:      0    1116    2166 
   Size:       1116    1050
 
HaeII r_GCGC'y

Cuts at:      0    1236    1522    2166 
   Size:       1236     286     644

  Fragments arranged by size: 

                1236     644     286
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     868     900    1486    1518    2047    2079    2166 
   Size:        868      32     586      32     529      32      87

  Fragments arranged by size: 

                 868     586     529      87      32      32      32
 
KasI G'GCGC_C

Cuts at:      0    1518    2166 
   Size:       1518     648
 
MfeI C'AATT_G

Cuts at:      0     495    2166 
   Size:        495    1671
 
MscI TGG'CCA

Cuts at:      0     839    1451    2166 
   Size:        839     612     715

  Fragments arranged by size: 

                 839     715     612
 
MslI CAynn'nnrTG

Cuts at:      0     163     329     592     751    1876    2166 
   Size:        163     166     263     159    1125     290

  Fragments arranged by size: 

                1125     290     263     166     163     159
 
MspA1I CmG'CkG

Cuts at:      0     652    1468    2166 
   Size:        652     816     698

  Fragments arranged by size: 

                 816     698     652
 
NaeI GCC'GGC

Cuts at:      0    1712    2166 
   Size:       1712     454
 
NarI GG'CG_CC

Cuts at:      0    1519    2166 
   Size:       1519     647
 
NcoI C'CATG_G

Cuts at:      0     587     942    1839    2166 
   Size:        587     355     897     327

  Fragments arranged by size: 

                 897     587     355     327
 
NdeI CA'TA_TG

Cuts at:      0    1935    2166 
   Size:       1935     231
 
NgoMIV G'CCGG_C

Cuts at:      0    1710    2166 
   Size:       1710     456
 
NspI r_CATG'y

Cuts at:      0     509     712    1050    2061    2166 
   Size:        509     203     338    1011     105

  Fragments arranged by size: 

                1011     509     338     203     105
 
PasI CC'CwG_GG

Cuts at:      0     615     622    2166 
   Size:        615       7    1544

  Fragments arranged by size: 

                1544     615       7
 
PciI A'CATG_T

Cuts at:      0    1046    2057    2166 
   Size:       1046    1011     109

  Fragments arranged by size: 

                1046    1011     109
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     450    1821    2166 
   Size:        450    1371     345

  Fragments arranged by size: 

                1371     450     345
 
PfoI T'CCnGG_A

Cuts at:      0    1241    2166 
   Size:       1241     925
 
PspOMI G'GGCC_C

Cuts at:      0     391     618    1907    2166 
   Size:        391     227    1289     259

  Fragments arranged by size: 

                1289     391     259     227
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     343     375    1177    1209    2166 
   Size:        343      32     802      32     957

  Fragments arranged by size: 

                 957     802     343      32      32
 
PvuII CAG'CTG

Cuts at:      0    1468    2166 
   Size:       1468     698
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2138    2166 
   Size:       2138      28
 
ScaI AGT'ACT

Cuts at:      0    2125    2166 
   Size:       2125      41
 
SexAI A'CCwGG_T

Cuts at:      0     353    2166 
   Size:        353    1813
 
SfoI GGC'GCC

Cuts at:      0    1520    2166 
   Size:       1520     646
 
SmlI C'TyrA_G

Cuts at:      0     736    2166 
   Size:        736    1430
 
SphI G_CATG'C

Cuts at:      0     509    2166 
   Size:        509    1657
 
StuI AGG'CCT

Cuts at:      0     223     734    1858    2166 
   Size:        223     511    1124     308

  Fragments arranged by size: 

                1124     511     308     223
 
StyI C'CwwG_G

Cuts at:      0     587     942    1839    2166 
   Size:        587     355     897     327

  Fragments arranged by size: 

                 897     587     355     327
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     163     955    1340    2166 
   Size:        163     792     385     826

  Fragments arranged by size: 

                 826     792     385     163
 
TatI w'GTAC_w

Cuts at:      0    2123    2166 
   Size:       2123      43
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      74     106    1601    1633    2166 
   Size:         74      32    1495      32     533

  Fragments arranged by size: 

                1495     533      74      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     822    1164    1232    2166 
   Size:        822     342      68     934

  Fragments arranged by size: 

                 934     822     342      68
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII     AleI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AvaI     BanI    BanII     BbeI    BbvCI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrFI    BssSI    BstBI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     DraI     EaeI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRV    HaeII    Hin4I     KasI     MfeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NspI     PasI     PciI    PflMI     PfoI   PspOMI     PsrI    PvuII
     SapI     ScaI    SexAI     SfoI     SmlI     SphI     StuI     StyI
    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AloI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI
     BcgI    BfrBI    BmgBI     BmtI     BplI    BsaBI    BseRI    BsiEI
    BsiWI     BsmI    BsmBI    BspHI    BsrDI    BsrGI   BssHII   BstAPI
   BstEII  BstZ17I     BtsI     ClaI    CspCI   DraIII     DrdI     EagI
  EcoICRI    EcoRI     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MluI     MmeI     NheI     NotI     NruI     NsiI
     PacI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     SfcI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     SwaI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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