Restriction map for pF1KA0829
 (Linear) (Six-base) MAPSORT of: KIAA0829.seq  Check: 8347  from: 1  to: 3690



KIAA0829.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:21  ..

AatII G_ACGT'C

Cuts at:      0     411    3690 
   Size:        411    3279
 
AccI GT'mk_AC

Cuts at:      0     537    2488    3391    3690 
   Size:        537    1951     903     299

  Fragments arranged by size: 

                1951     903     537     299
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1267    1631    1819    3690 
   Size:       1267     364     188    1871

  Fragments arranged by size: 

                1871    1267     364     188
 
AflII C'TTAA_G

Cuts at:      0    2550    2908    3690 
   Size:       2550     358     782

  Fragments arranged by size: 

                2550     782     358
 
AflIII A'CryG_T

Cuts at:      0    1367    3454    3690 
   Size:       1367    2087     236

  Fragments arranged by size: 

                2087    1367     236
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     298    3690 
   Size:        298    3392
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1937    1969    3690 
   Size:       1937      32    1721

  Fragments arranged by size: 

                1937    1721      32
 
ApaI G_GGCC'C

Cuts at:      0    1404    3690 
   Size:       1404    2286
 
ApoI r'AATT_y

Cuts at:      0     195     784    1108    1473    2968    3310    3589    3690 
   Size:        195     589     324     365    1495     342     279     101

  Fragments arranged by size: 

                1495     589     365     342     324     279     195     101
            
 
AseI AT'TA_AT

Cuts at:      0    3118    3690 
   Size:       3118     572
 
AvaI C'yCGr_G

Cuts at:      0    2420    3690 
   Size:       2420    1270
 
BamHI G'GATC_C

Cuts at:      0    2194    3690 
   Size:       2194    1496
 
BanII G_rGCy'C

Cuts at:      0    1404    2090    2253    3690 
   Size:       1404     686     163    1437

  Fragments arranged by size: 

                1437    1404     686     163
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1351    1527    3053    3690 
   Size:       1351     176    1526     637

  Fragments arranged by size: 

                1526    1351     637     176
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2305    3129    3652    3690 
   Size:       2305     824     523      38

  Fragments arranged by size: 

                2305     824     523      38
 
BclI T'GATC_A

Cuts at:      0     993    1743    3690 
   Size:        993     750    1947

  Fragments arranged by size: 

                1947     993     750
 
BfrBI ATG'CAT

Cuts at:      0    2118    2635    3690 
   Size:       2118     517    1055

  Fragments arranged by size: 

                2118    1055     517
 
BglII A'GATC_T

Cuts at:      0     636    1698    3690 
   Size:        636    1062    1992

  Fragments arranged by size: 

                1992    1062     636
 
Bme1580I G_kGCm'C

Cuts at:      0    1404    3690 
   Size:       1404    2286
 
BmgBI CAC'GTC

Cuts at:      0    1051    3690 
   Size:       1051    2639
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     540    2011    3690 
   Size:        540    1471    1679

  Fragments arranged by size: 

                1679    1471     540
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2381    3690 
   Size:       2381    1309
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     338    1550    3690 
   Size:        338    1212    2140

  Fragments arranged by size: 

                2140    1212     338
 
Bpu10I CC'TnA_GC

Cuts at:      0    3617    3690 
   Size:       3617      73
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     778    1514    2466    3690 
   Size:        778     736     952    1224

  Fragments arranged by size: 

                1224     952     778     736
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1580    3690 
   Size:       1580    2110
 
BsaAI yAC'GTr

Cuts at:      0    1506    3448    3690 
   Size:       1506    1942     242

  Fragments arranged by size: 

                1942    1506     242
 
BsaBI GATnn'nnATC

Cuts at:      0     992    3690 
   Size:        992    2698
 
BsaHI Gr'CG_yC

Cuts at:      0     408    3690 
   Size:        408    3282
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1110    1140    1937    1967    3690 
   Size:       1110      30     797      30    1723

  Fragments arranged by size: 

                1723    1110     797      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1517    2163    3690 
   Size:       1517     646    1527

  Fragments arranged by size: 

                1527    1517     646
 
BseYI C'CCAG_C

Cuts at:      0    1906    3690 
   Size:       1906    1784
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     258    1073    1115    2846    3428    3690 
   Size:        258     815      42    1731     582     262

  Fragments arranged by size: 

                1731     815     582     262     258      42
 
BsiHKAI G_wGCw'C

Cuts at:      0    2090    2253    2362    3690 
   Size:       2090     163     109    1328

  Fragments arranged by size: 

                2090    1328     163     109
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1041    1128    2509    3690 
   Size:       1041      87    1381    1181

  Fragments arranged by size: 

                1381    1181    1041      87
 
Bsp1286I G_dGCh'C

Cuts at:      0    1404    2090    2253    2362    3690 
   Size:       1404     686     163     109    1328

  Fragments arranged by size: 

                1404    1328     686     163     109
 
BsrDI GCAATG_nn'

Cuts at:      0     798     971    1256    2393    3690 
   Size:        798     173     285    1137    1297

  Fragments arranged by size: 

                1297    1137     798     285     173
 
BsrGI T'GTAC_A

Cuts at:      0    1221    3266    3457    3690 
   Size:       1221    2045     191     233

  Fragments arranged by size: 

                2045    1221     233     191
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     379     970    1098    2632    3690 
   Size:        379     591     128    1534    1058

  Fragments arranged by size: 

                1534    1058     591     379     128
 
BstEII G'GTnAC_C

Cuts at:      0    1910    3690 
   Size:       1910    1780
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      33     120    3690 
   Size:         33      87    3570

  Fragments arranged by size: 

                3570      87      33
 
BstYI r'GATC_y

Cuts at:      0     636    1698    2194    3126    3690 
   Size:        636    1062     496     932     564

  Fragments arranged by size: 

                1062     932     636     564     496
 
Bsu36I CC'TnA_GG

Cuts at:      0     860    3690 
   Size:        860    2830
 
BtgI C'CryG_G

Cuts at:      0    2902    3690 
   Size:       2902     788
 
BtsI GCAGTG_nn'

Cuts at:      0     575    3690 
   Size:        575    3115
 
ClaI AT'CG_AT

Cuts at:      0    1484    3690 
   Size:       1484    2206
 
DraI TTT'AAA

Cuts at:      0    1149    3213    3690 
   Size:       1149    2064     477

  Fragments arranged by size: 

                2064    1149     477
 
EaeI y'GGCC_r

Cuts at:      0     597    2132    3690 
   Size:        597    1535    1558

  Fragments arranged by size: 

                1558    1535     597
 
EarI CTCTTCn'nnn_

Cuts at:      0    1154    1319    3063    3565    3690 
   Size:       1154     165    1744     502     125

  Fragments arranged by size: 

                1744    1154     502     165     125
 
EcoICRI GAG'CTC

Cuts at:      0    2088    2251    3690 
   Size:       2088     163    1439

  Fragments arranged by size: 

                2088    1439     163
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     338    1267    1550    1631    1819    3690 
   Size:        338     929     283      81     188    1871

  Fragments arranged by size: 

                1871     929     338     283     188      81
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1400    1401    3690 
   Size:       1400       1    2289

  Fragments arranged by size: 

                2289    1400       1
 
EcoRI G'AATT_C

Cuts at:      0    1108    3589    3690 
   Size:       1108    2481     101

  Fragments arranged by size: 

                2481    1108     101
 
EcoRV GAT'ATC

Cuts at:      0    2924    3303    3690 
   Size:       2924     379     387

  Fragments arranged by size: 

                2924     387     379
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     319     517    1636    2934    3690 
   Size:        319     198    1119    1298     756

  Fragments arranged by size: 

                1298    1119     756     319     198
 
FspI TGC'GCA

Cuts at:      0    2329    3690 
   Size:       2329    1361
 
HaeII r_GCGC'y

Cuts at:      0      11    3690 
   Size:         11    3679
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     667     699    1110    1142    1477    1509    2186    2218 
   Size:        667      32     411      32     335      32     677      32

Cuts at:   2218    3287    3319    3643    3675    3690 
   Size:       1069      32     324      32      15

  Fragments arranged by size: 

                1069     677     667     411     335     324      32      32
                  32      32      32      32      15
 
HincII GTy'rAC

Cuts at:      0     551     686    1373    1877    2704    3690 
   Size:        551     135     687     504     827     986

  Fragments arranged by size: 

                 986     827     687     551     504     135
 
HindIII A'AGCT_T

Cuts at:      0    2588    3690 
   Size:       2588    1102
 
HpaI GTT'AAC

Cuts at:      0    1373    1877    3690 
   Size:       1373     504    1813

  Fragments arranged by size: 

                1813    1373     504
 
MfeI C'AATT_G

Cuts at:      0     257    3690 
   Size:        257    3433
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     101    1018    1563    2543    3404    3690 
   Size:        101     917     545     980     861     286

  Fragments arranged by size: 

                 980     917     861     545     286     101
 
MscI TGG'CCA

Cuts at:      0     599    2134    3690 
   Size:        599    1535    1556

  Fragments arranged by size: 

                1556    1535     599
 
MslI CAynn'nnrTG

Cuts at:      0     280    2650    3258    3690 
   Size:        280    2370     608     432

  Fragments arranged by size: 

                2370     608     432     280
 
MspA1I CmG'CkG

Cuts at:      0    1057    1735    2059    2446    2623    3690 
   Size:       1057     678     324     387     177    1067

  Fragments arranged by size: 

                1067    1057     678     387     324     177
 
NdeI CA'TA_TG

Cuts at:      0    2781    2937    3690 
   Size:       2781     156     753

  Fragments arranged by size: 

                2781     753     156
 
NsiI A_TGCA'T

Cuts at:      0    2120    2637    3690 
   Size:       2120     517    1053

  Fragments arranged by size: 

                2120    1053     517
 
NspI r_CATG'y

Cuts at:      0     285    1371    2122    2334    2429    3458    3690 
   Size:        285    1086     751     212      95    1029     232

  Fragments arranged by size: 

                1086    1029     751     285     232     212      95
 
PciI A'CATG_T

Cuts at:      0    1367    3454    3690 
   Size:       1367    2087     236

  Fragments arranged by size: 

                2087    1367     236
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2283    3690 
   Size:       2283    1407
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1937    1969    3690 
   Size:       1937      32    1721

  Fragments arranged by size: 

                1937    1721      32
 
PshAI GACnn'nnGTC

Cuts at:      0    3284    3690 
   Size:       3284     406
 
PsiI TTA'TAA

Cuts at:      0     892     932    3187    3690 
   Size:        892      40    2255     503

  Fragments arranged by size: 

                2255     892     503      40
 
PspOMI G'GGCC_C

Cuts at:      0    1400    3690 
   Size:       1400    2290
 
PspXI vC'TCGA_Gb

Cuts at:      0    2420    3690 
   Size:       2420    1270
 
PstI C_TGCA'G

Cuts at:      0     104    3419    3690 
   Size:        104    3315     271

  Fragments arranged by size: 

                3315     271     104
 
PvuII CAG'CTG

Cuts at:      0    1057    1735    2059    2446    2623    3690 
   Size:       1057     678     324     387     177    1067

  Fragments arranged by size: 

                1067    1057     678     387     324     177
 
SacI G_AGCT'C

Cuts at:      0    2090    2253    3690 
   Size:       2090     163    1437

  Fragments arranged by size: 

                2090    1437     163
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1319    3690 
   Size:       1319    2371
 
ScaI AGT'ACT

Cuts at:      0     820    3413    3690 
   Size:        820    2593     277

  Fragments arranged by size: 

                2593     820     277
 
SexAI A'CCwGG_T

Cuts at:      0    1870    3690 
   Size:       1870    1820
 
SfcI C'TryA_G

Cuts at:      0     100    1076    1880    2040    2447    2489    3415    3690 
   Size:        100     976     804     160     407      42     926     275

  Fragments arranged by size: 

                 976     926     804     407     275     160     100      42
            
 
SmlI C'TyrA_G

Cuts at:      0     757    1529    2420    2481    2550    2908    3690 
   Size:        757     772     891      61      69     358     782

  Fragments arranged by size: 

                 891     782     772     757     358      69      61
 
SnaBI TAC'GTA

Cuts at:      0    1506    3690 
   Size:       1506    2184
 
SpeI A'CTAG_T

Cuts at:      0    2698    3685    3690 
   Size:       2698     987       5

  Fragments arranged by size: 

                2698     987       5
 
SphI G_CATG'C

Cuts at:      0    2334    2429    3690 
   Size:       2334      95    1261

  Fragments arranged by size: 

                2334    1261      95
 
SspI AAT'ATT

Cuts at:      0     897    3690 
   Size:        897    2793
 
StuI AGG'CCT

Cuts at:      0    1935    3690 
   Size:       1935    1755
 
StyI C'CwwG_G

Cuts at:      0     461    1797    3077    3690 
   Size:        461    1336    1280     613

  Fragments arranged by size: 

                1336    1280     613     461
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3443    3690 
   Size:       3443     247
 
TatI w'GTAC_w

Cuts at:      0     818    1221    1422    3266    3411    3457    3690 
   Size:        818     403     201    1844     145      46     233

  Fragments arranged by size: 

                1844     818     403     233     201     145      46
 
XbaI T'CTAG_A

Cuts at:      0    2079    3276    3690 
   Size:       2079    1197     414

  Fragments arranged by size: 

                2079    1197     414
 
XhoI C'TCGA_G

Cuts at:      0    2420    3690 
   Size:       2420    1270
 
XmnI GAAnn'nnTTC

Cuts at:      0     302    1100    1616    1952    3690 
   Size:        302     798     516     336    1738

  Fragments arranged by size: 

                1738     798     516     336     302
 
ZraI GAC'GTC

Cuts at:      0     409    3690 
   Size:        409    3281
 

 Enzymes that do cut:

    AatII     AccI     AcuI    AflII   AflIII     AlfI     AloI     ApaI
     ApoI     AseI     AvaI    BamHI    BanII     BbsI    BciVI     BclI
    BfrBI    BglII Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI    BsmBI Bsp1286I    BsrDI    BsrGI   BstAPI   BstEII
    BstXI    BstYI   Bsu36I     BtgI     BtsI     ClaI     DraI     EaeI
     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     MfeI     MmeI     MscI
     MslI   MspA1I     NdeI     NsiI     NspI     PciI    PflMI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PstI    PvuII     SacI     SapI
     ScaI    SexAI     SfcI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     XbaI     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI     AgeI     AhdI     AleI    AlwNI
    ApaLI     AscI    AsiSI    AvrII     BaeI     BanI     BbeI    BbvCI
     BcgI     BglI     BlpI     BmtI    BsaWI    BsiEI    BsiWI     BsmI
    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI    BstBI
  BstZ17I    BtgZI    CspCI   DraIII     DrdI     EagI     EciI    EcoNI
     FseI    FspAI     KasI     KpnI     MluI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PfoI     PmeI
     PmlI    PpuMI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI     SrfI     SwaI
     TstI  Tth111I     XcmI     XmaI
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