Restriction map for pF1KA0791
 (Linear) (Six-base) MAPSORT of: KIAA0791.seq  Check: 7322  from: 1  to: 3996



KIAA0791.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     904    3996 
   Size:        904    3092
 
AccI GT'mk_AC

Cuts at:      0     335    1701    2960    3801    3996 
   Size:        335    1366    1259     841     195

  Fragments arranged by size: 

                1366    1259     841     335     195
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     569    2140    2236    2296    2692    3139    3271    3996 
   Size:        569    1571      96      60     396     447     132     725

  Fragments arranged by size: 

                1571     725     569     447     396     132      96      60
            
 
AflIII A'CryG_T

Cuts at:      0    1301    3804    3996 
   Size:       1301    2503     192

  Fragments arranged by size: 

                2503    1301     192
 
AlwNI CAG_nnn'CTG

Cuts at:      0     382    2055    2109    2196    3996 
   Size:        382    1673      54      87    1800

  Fragments arranged by size: 

                1800    1673     382      87      54
 
ApoI r'AATT_y

Cuts at:      0     497     870    1318    1978    3146    3238    3394    3581 
   Size:        497     373     448     660    1168      92     156     187

Cuts at:   3581    3957    3996 
   Size:        376      39

  Fragments arranged by size: 

                1168     660     497     448     376     373     187     156
                  92      39
 
AseI AT'TA_AT

Cuts at:      0    2332    3996 
   Size:       2332    1664
 
AvaI C'yCGr_G

Cuts at:      0    1532    3179    3996 
   Size:       1532    1647     817

  Fragments arranged by size: 

                1647    1532     817
 
BamHI G'GATC_C

Cuts at:      0    3278    3753    3996 
   Size:       3278     475     243

  Fragments arranged by size: 

                3278     475     243
 
BanII G_rGCy'C

Cuts at:      0     601    1056    1203    2924    3532    3914    3928    3996 
   Size:        601     455     147    1721     608     382      14      68

  Fragments arranged by size: 

                1721     608     601     455     382     147      68      14
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1460    2699    3996 
   Size:       1460    1239    1297

  Fragments arranged by size: 

                1460    1297    1239
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2158    2192    3996 
   Size:       2158      34    1804

  Fragments arranged by size: 

                2158    1804      34
 
BclI T'GATC_A

Cuts at:      0    3033    3732    3996 
   Size:       3033     699     264

  Fragments arranged by size: 

                3033     699     264
 
BfrBI ATG'CAT

Cuts at:      0    2744    3135    3546    3996 
   Size:       2744     391     411     450

  Fragments arranged by size: 

                2744     450     411     391
 
BglI GCCn_nnn'nGGC

Cuts at:      0     544    3996 
   Size:        544    3452
 
BglII A'GATC_T

Cuts at:      0    2295    3996 
   Size:       2295    1701
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1992    2795    3669    3962    3996 
   Size:       1992     803     874     293      34

  Fragments arranged by size: 

                1992     874     803     293      34
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     103     187    3996 
   Size:        103      84    3809

  Fragments arranged by size: 

                3809     103      84
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     404    1709    2449    2810    3022    3898    3996 
   Size:        404    1305     740     361     212     876      98

  Fragments arranged by size: 

                1305     876     740     404     361     212      98
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2633    3544    3996 
   Size:       2633     911     452

  Fragments arranged by size: 

                2633     911     452
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     212    1625    3996 
   Size:        212    1413    2371

  Fragments arranged by size: 

                2371    1413     212
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1978    2008    3996 
   Size:       1978      30    1988

  Fragments arranged by size: 

                1988    1978      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     230    1318    1424    2780    3522    3996 
   Size:        230    1088     106    1356     742     474

  Fragments arranged by size: 

                1356    1088     742     474     230     106
 
BseYI C'CCAG_C

Cuts at:      0    1746    2090    3835    3996 
   Size:       1746     344    1745     161

  Fragments arranged by size: 

                1746    1745     344     161
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2065    3493    3769    3996 
   Size:       2065    1428     276     227

  Fragments arranged by size: 

                2065    1428     276     227
 
BsiEI CG_ry'CG

Cuts at:      0     828    3996 
   Size:        828    3168
 
BsiHKAI G_wGCw'C

Cuts at:      0     601    1056    1737    2577    2924    3532    3914    3928 
   Size:        601     455     681     840     347     608     382      14

Cuts at:   3928    3996 
   Size:         68

  Fragments arranged by size: 

                 840     681     608     601     455     382     347      68
                  14
 
BsmI GAATG_Cn'

Cuts at:      0     419     789    3548    3996 
   Size:        419     370    2759     448

  Fragments arranged by size: 

                2759     448     419     370
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1697    3996 
   Size:       1697    2299
 
Bsp1286I G_dGCh'C

Cuts at:      0     601    1056    1203    1737    2577    2924    3532    3914 
   Size:        601     455     147     534     840     347     608     382

Cuts at:   3914    3928    3996 
   Size:         14      68

  Fragments arranged by size: 

                 840     608     601     534     455     382     347     147
                  68      14
 
BspHI T'CATG_A

Cuts at:      0    2124    3246    3996 
   Size:       2124    1122     750

  Fragments arranged by size: 

                2124    1122     750
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     904    3633    3996 
   Size:        904    2729     363

  Fragments arranged by size: 

                2729     904     363
 
BsrBI CCG'CTC

Cuts at:      0    2788    3996 
   Size:       2788    1208
 
BsrDI GCAATG_nn'

Cuts at:      0     809    3173    3706    3996 
   Size:        809    2364     533     290

  Fragments arranged by size: 

                2364     809     533     290
 
BsrFI r'CCGG_y

Cuts at:      0    3448    3996 
   Size:       3448     548
 
BsrGI T'GTAC_A

Cuts at:      0    1290    3333    3624    3996 
   Size:       1290    2043     291     372

  Fragments arranged by size: 

                2043    1290     372     291
 
BssSI C'ACGA_G

Cuts at:      0    3107    3996 
   Size:       3107     889
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2335    3996 
   Size:       2335    1661
 
BstBI TT'CG_AA

Cuts at:      0    2178    3996 
   Size:       2178    1818
 
BstEII G'GTnAC_C

Cuts at:      0    3898    3996 
   Size:       3898      98
 
BstYI r'GATC_y

Cuts at:      0     429    2295    2643    3278    3753    3996 
   Size:        429    1866     348     635     475     243

  Fragments arranged by size: 

                1866     635     475     429     348     243
 
BstZ17I GTA'TAC

Cuts at:      0    1702    3802    3996 
   Size:       1702    2100     194

  Fragments arranged by size: 

                2100    1702     194
 
BtgI C'CryG_G

Cuts at:      0    1025    3996 
   Size:       1025    2971
 
BtsI GCAGTG_nn'

Cuts at:      0     130     866    1493    1907    3206    3781    3996 
   Size:        130     736     627     414    1299     575     215

  Fragments arranged by size: 

                1299     736     627     575     414     215     130
 
ClaI AT'CG_AT

Cuts at:      0     824    3996 
   Size:        824    3172
 
DraI TTT'AAA

Cuts at:      0     840    1250    1440    2292    3411    3848    3963    3996 
   Size:        840     410     190     852    1119     437     115      33

  Fragments arranged by size: 

                1119     852     840     437     410     190     115      33
            
 
EaeI y'GGCC_r

Cuts at:      0    3023    3996 
   Size:       3023     973
 
EarI CTCTTCn'nnn_

Cuts at:      0      72     449    1415    1589    3974    3996 
   Size:         72     377     966     174    2385      22

  Fragments arranged by size: 

                2385     966     377     174      72      22
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1577    3996 
   Size:       1577    2419
 
EcoICRI GAG'CTC

Cuts at:      0     599    1054    2922    3530    3912    3926    3996 
   Size:        599     455    1868     608     382      14      70

  Fragments arranged by size: 

                1868     608     599     455     382      70      14
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     404     569    1709    2140    2236    2296    2449    2692 
   Size:        404     165    1140     431      96      60     153     243

Cuts at:   2692    2810    3022    3139    3271    3898    3996 
   Size:        118     212     117     132     627      98

  Fragments arranged by size: 

                1140     627     431     404     243     212     165     153
                 132     118     117      98      96      60
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      23    3996 
   Size:         23    3973
 
EcoO109I rG'GnC_Cy

Cuts at:      0      28    3996 
   Size:         28    3968
 
EcoRV GAT'ATC

Cuts at:      0    2406    3492    3996 
   Size:       2406    1086     504

  Fragments arranged by size: 

                2406    1086     504
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2575    2980    3996 
   Size:       2575     405    1016

  Fragments arranged by size: 

                2575    1016     405
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     388     420     810     842    1978    2010    3901    3933 
   Size:        388      32     390      32    1136      32    1891      32

Cuts at:   3933    3996 
   Size:         63

  Fragments arranged by size: 

                1891    1136     390     388      63      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1125    1721    2961    3996 
   Size:       1125     596    1240    1035

  Fragments arranged by size: 

                1240    1125    1035     596
 
HindIII A'AGCT_T

Cuts at:      0    1863    3382    3996 
   Size:       1863    1519     614

  Fragments arranged by size: 

                1863    1519     614
 
MfeI C'AATT_G

Cuts at:      0     176     671     845    1913    3996 
   Size:        176     495     174    1068    2083

  Fragments arranged by size: 

                2083    1068     495     176     174
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1600    2682    3306    3996 
   Size:       1600    1082     624     690

  Fragments arranged by size: 

                1600    1082     690     624
 
MscI TGG'CCA

Cuts at:      0    3025    3996 
   Size:       3025     971
 
MslI CAynn'nnrTG

Cuts at:      0     914    1765    3996 
   Size:        914     851    2231

  Fragments arranged by size: 

                2231     914     851
 
MspA1I CmG'CkG

Cuts at:      0    1177    2274    2374    3354    3996 
   Size:       1177    1097     100     980     642

  Fragments arranged by size: 

                1177    1097     980     642     100
 
NdeI CA'TA_TG

Cuts at:      0     119    3996 
   Size:        119    3877
 
NsiI A_TGCA'T

Cuts at:      0    2746    3137    3548    3996 
   Size:       2746     391     411     448

  Fragments arranged by size: 

                2746     448     411     391
 
NspI r_CATG'y

Cuts at:      0     913    1305    3135    3550    3808    3996 
   Size:        913     392    1830     415     258     188

  Fragments arranged by size: 

                1830     913     415     392     258     188
 
PciI A'CATG_T

Cuts at:      0    1301    3804    3996 
   Size:       1301    2503     192

  Fragments arranged by size: 

                2503    1301     192
 
PfoI T'CCnGG_A

Cuts at:      0    1002    3996 
   Size:       1002    2994
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1677    1709    3996 
   Size:       1677      32    2287

  Fragments arranged by size: 

                2287    1677      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      28    3996 
   Size:         28    3968
 
PsiI TTA'TAA

Cuts at:      0    2942    3996 
   Size:       2942    1054
 
PspXI vC'TCGA_Gb

Cuts at:      0    3179    3996 
   Size:       3179     817
 
PstI C_TGCA'G

Cuts at:      0    2114    2634    3996 
   Size:       2114     520    1362

  Fragments arranged by size: 

                2114    1362     520
 
PvuI CG_AT'CG

Cuts at:      0     828    3996 
   Size:        828    3168
 
PvuII CAG'CTG

Cuts at:      0    1177    2274    3354    3996 
   Size:       1177    1097    1080     642

  Fragments arranged by size: 

                1177    1097    1080     642
 
SacI G_AGCT'C

Cuts at:      0     601    1056    2924    3532    3914    3928    3996 
   Size:        601     455    1868     608     382      14      68

  Fragments arranged by size: 

                1868     608     601     455     382      68      14
 
SalI G'TCGA_C

Cuts at:      0    2959    3996 
   Size:       2959    1037
 
SexAI A'CCwGG_T

Cuts at:      0      30    2817    3996 
   Size:         30    2787    1179

  Fragments arranged by size: 

                2787    1179      30
 
SfcI C'TryA_G

Cuts at:      0     332    1370    2110    2630    2980    3996 
   Size:        332    1038     740     520     350    1016

  Fragments arranged by size: 

                1038    1016     740     520     350     332
 
SmlI C'TyrA_G

Cuts at:      0    2648    3179    3559    3996 
   Size:       2648     531     380     437

  Fragments arranged by size: 

                2648     531     437     380
 
SphI G_CATG'C

Cuts at:      0    3550    3996 
   Size:       3550     446
 
SspI AAT'ATT

Cuts at:      0     690    1883    3996 
   Size:        690    1193    2113

  Fragments arranged by size: 

                2113    1193     690
 
StuI AGG'CCT

Cuts at:      0    2713    3996 
   Size:       2713    1283
 
StyI C'CwwG_G

Cuts at:      0    2912    3573    3996 
   Size:       2912     661     423

  Fragments arranged by size: 

                2912     661     423
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     130    3996 
   Size:        130    3866
 
TatI w'GTAC_w

Cuts at:      0    1044    1290    1796    3111    3170    3333    3624    3649 
   Size:       1044     246     506    1315      59     163     291      25

Cuts at:   3649    3939    3996 
   Size:        290      57

  Fragments arranged by size: 

                1315    1044     506     291     290     246     163      59
                  57      25
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     593     625    2999    3031    3996 
   Size:        593      32    2374      32     965

  Fragments arranged by size: 

                2374     965     593      32      32
 
XbaI T'CTAG_A

Cuts at:      0     682    1983    3996 
   Size:        682    1301    2013

  Fragments arranged by size: 

                2013    1301     682
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1174    3996 
   Size:       1174    2822
 
XhoI C'TCGA_G

Cuts at:      0    3179    3996 
   Size:       3179     817
 
XmnI GAAnn'nnTTC

Cuts at:      0    3242    3989    3996 
   Size:       3242     747       7

  Fragments arranged by size: 

                3242     747       7
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII    AlwNI     ApoI     AseI     AvaI
    BamHI    BanII     BbsI     BcgI     BclI    BfrBI     BglI    BglII
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I     BtgI     BtsI     ClaI     DraI     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI    Hin4I
   HincII  HindIII     MfeI     MmeI     MscI     MslI   MspA1I     NdeI
     NsiI     NspI     PciI     PfoI     PpiI    PpuMI     PsiI    PspXI
     PstI     PvuI    PvuII     SacI     SalI    SexAI     SfcI     SmlI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI     XbaI
     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI
     BanI     BbeI    BbvCI    BciVI     BlpI Bme1580I    BmgBI     BmtI
   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BsiWI    BspEI   BssHII
    BstXI   Bsu36I    BtgZI    CspCI   DraIII     DrdI     EagI    EcoRI
     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MluI
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     PacI
     PasI    PflMI     PmeI     PmlI    PshAI   PspOMI     PsrI    RsrII
    SacII    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SwaI  Tth111I     XmaI
     ZraI
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