Restriction map for pF1KA0706
##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        Alw44I      ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvrII       BamHI       BbrPI       BbvCI       BciVI       
BclI        BfrI        BlnI        BsaBI       BsiEI       
BsmI        Bsp1407I    BspDI       BspLU11I    BspMI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     ClaI        CpoI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HpaI        KpnI        KspI        
MamI        MfeI        MluI        MunI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RsrII       SacI        SacII       SapI        SbfI        
ScaI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
Van91I      XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI         1864  GTCGAC               GT^[AC][GT]AC
AccII         385  CGCG                 CG^CG
AccIII       1637  TCCGGA               T^CCGGA
AcsI          933  AAATTT               [GA]^AATT[CT]
AflIII        269  ACGTGT               A^C[GA][CT]GT
ApoI          933  AAATTT               [GA]^AATT[CT]
AviII        2885  TGCGCA               TGC^GCA
BalI         2971  TGGCCA               TGG^CCA
BbeI         1195  GGCGCC               GGCGC^C
BcgI          213  GCAAGTGTATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          832  CTAG                 C^TAG
BsaAI         529  TACGTG               [CT]AC^GT[GA]
BseAI        1637  TCCGGA               T^CCGGA
BsiWI         527  CGTACG               C^GTACG
BspEI        1637  TCCGGA               T^CCGGA
BspHI        2631  TCATGA               T^CATGA
BsrFI        3053  GCCGGC               [GA]^CCGG[CT]
BstUI         385  CGCG                 CG^CG
Cfr10I       3053  GCCGGC               [GA]^CCGG[CT]
DraIII       1498  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
FspI         2885  TGCGCA               TGC^GCA
HincII       1864  GTCGAC               GT[CT]^[GA]AC
HindII       1864  GTCGAC               GT[CT]^[GA]AC
KasI         1195  GGCGCC               G^GCGCC
MaeI          832  CTAG                 C^TAG
MluNI        2971  TGGCCA               TGG^CCA
MroI         1637  TCCGGA               T^CCGGA
MscI         2971  TGGCCA               TGG^CCA
MseI           44  TTAA                 T^TAA
MvnI          385  CGCG                 CG^CG
NaeI         3053  GCCGGC               GCC^GGC
NarI         1195  GGCGCC               GG^CGCC
NdeI         1703  CATATG               CA^TATG
NgoMIV       3053  GCCGGC               G^CCGGC
PshAI        2115  GACCATTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         2631  TCATGA               T^CATGA
SalI         1864  GTCGAC               G^TCGAC
SexAI        1499  ACCTGGT              A^CC[AT]GGT
SfiI         3215  GGCCCCCAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1195  GGCGCC               GGC^GCC
StuI         1164  AGGCCT               AGG^CCT
Tru9I          44  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

Aor51HI       680  AGCGCT               AGC^GCT
             2045  AGCGCT              
BanI            7  GGTGCC               G^G[CT][GA]CC
             1195  GGCGCC              
BglII         443  AGATCT               A^GATCT
             2261  AGATCT              
BlpI          406  GCTCAGC              GC^T[ACGT]AGC
             1354  GCTGAGC             
Bpu1102I      406  GCTCAGC              GC^T[ACGT]AGC
             1354  GCTGAGC             
BsgI         3134  CTGCAC               GTGCAG(16/14)
              532  GTGCAG              
BsmBI        2378  GAGACG               CGTCTC(1/5)
             3261  GAGACG              
CelII         406  GCTCAGC              GC^T[ACGT]AGC
             1354  GCTGAGC             
Eco47III      680  AGCGCT               AGC^GCT
             2045  AGCGCT              
EcoNI        1684  CCTTGTGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2619  CCTGGCCCAGG         
EcoT14I      1319  CCATGG               C^C[AT][AT]GG
             2228  CCATGG              
HindIII       144  AAGCTT               A^AGCTT
             1717  AAGCTT              
NcoI         1319  CCATGG               C^CATGG
             2228  CCATGG              
StyI         1319  CCATGG               C^C[AT][AT]GG
             2228  CCATGG              
TfiI         1953  GAATC                G^A[AT]TC
             2526  GATTC               
XcmI         1548  CCACATCAAAGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2749  CCACTGTCAAGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  106 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       AocI        ApaI        AspHI       AvaI        
AvaII       BanII       BbsI        BbvI        BcnI        
BglI        BmyI        BpmI        BpuAI       BsaHI       
BsaI        BsaJI       BsaWI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrBI       BsrDI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstXI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco81I      EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
SmlI        SphI        TaqI        TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmaCI       
XmaI        
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