Restriction map for pF1KA0674
##################################
# NO CUT ENZYMES (   95 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AhdI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       BamHI       BbeI        BbrPI       BclI        
BfrI        BsaAI       BsaBI       BseAI       BsiEI       
BsiWI       Bsp1407I    BspEI       BspLU11I    BsrGI       
BssHII      BssSI       Bst1107I    BstEII      BstPI       
BstZ17I     CpoI        DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRI       
EcoRV       FbaI        FseI        HaeII       HindIII     
HpaI        KasI        KpnI        KspI        MaeII       
MamI        MfeI        MluI        MroI        MunI        
NarI        NdeI        NheI        NotI        NruI        
PacI        PflMI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        ScaI        SfiI        
SfoI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Van91I      XbaI        XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccII         913  CGCG                 CG^CG
AcyI         3593  GACGCC               G[GA]^CG[CT]C
AgeI         3394  ACCGGT               A^CCGGT
ApaI         3115  GGGCCC               GGGCC^C
Asp700I      2858  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1212  GACTCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        3144  CCTAGG               C^CTAGG
BalI           96  TGGCCA               TGG^CCA
BciVI        3154  GTATCC               GTATCC(6/5)
BcnI         1535  CCCGG                CC^[GC]GG
BglII        2040  AGATCT               A^GATCT
BlnI         3144  CCTAGG               C^CTAGG
BsaHI        3593  GACGCC               G[GA]^CG[CT]C
BsaI         1953  GGTCTC               GGTCTC(1/5)
BsgI         2068  GTGCAG               GTGCAG(16/14)
BsmBI         929  CGTCTC               CGTCTC(1/5)
BsmI          731  GAATGC               GAATGC(1/-1)
BspDI         248  ATCGAT               AT^CGAT
BsrDI         504  CATTGC               GCAATG(2/0)
BstBI        1556  TTCGAA               TT^CGAA
BstUI         913  CGCG                 CG^CG
ClaI          248  ATCGAT               AT^CGAT
DraI         3514  TTTAAA               TTT^AAA
DrdI         2343  GACTCGAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I       242  ATGCAT               ATGCA^T
HgaI         3593  GACGC                GACGC(5/10)
Hin1I        3593  GACGCC               G[GA]^CG[CT]C
HincII        347  GTCAAC               GT[CT]^[GA]AC
HindII        347  GTCAAC               GT[CT]^[GA]AC
MluNI          96  TGGCCA               TGG^CCA
MscI           96  TGGCCA               TGG^CCA
MvnI          913  CGCG                 CG^CG
NaeI         1239  GCCGGC               GCC^GGC
NciI         1535  CCCGG                CC^[GC]GG
NgoMIV       1239  GCCGGC               G^CCGGC
NsiI          242  ATGCAT               ATGCA^T
NspV         1556  TTCGAA               TT^CGAA
PaeR7I       2345  CTCGAG               C^TCGAG
PflFI        1212  GACTCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        3394  ACCGGT               A^CCGGT
SacI         2086  GAGCTC               GAGCT^C
SexAI        2675  ACCAGGT              A^CC[AT]GGT
SfuI         1556  TTCGAA               TT^CGAA
SphI         1896  GCATGC               GCATG^C
Tth111I      1212  GACTCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         2345  CTCGAG               C^TCGAG
XmnI         2858  GAAGACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AvaI         2345  CTCGAG               C^[CT]CG[GA]G
             3304  CCCGAG              
AviII        1947  TGCGCA               TGC^GCA
             2482  TGCGCA              
BbsI         2858  GAAGAC               GAAGAC(2/6)
             3571  GAAGAC              
BlpI         2395  GCTGAGC              GC^T[ACGT]AGC
             3205  GCTAAGC             
Bpu1102I     2395  GCTGAGC              GC^T[ACGT]AGC
             3205  GCTAAGC             
BpuAI        2858  GAAGAC               GAAGAC(2/6)
             3571  GAAGAC              
BsaWI        2141  ACCGGA               [AT]^CCGG[AT]
             3394  ACCGGT              
BsoBI        2345  CTCGAG               C^[CT]CG[GA]G
             3304  CCCGAG              
BstAPI        235  GCAGTCCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1306  GCACCTTCTGC         
BstYI        1739  AGATCC               [GA]^GATC[CT]
             2040  AGATCT              
CelII        2395  GCTGAGC              GC^T[ACGT]AGC
             3205  GCTAAGC             
CfoI         1948  GCGC                 GCG^C
             2483  GCGC                
EcoNI         139  CCTAAGAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2172  CCTGGAGGAGG         
FspI         1947  TGCGCA               TGC^GCA
             2482  TGCGCA              
HhaI         1948  GCGC                 GCG^C
             2483  GCGC                
HinP1I       1948  GCGC                 G^CGC
             2483  GCGC                
MflI         1739  AGATCC               [GA]^GATC[CT]
             2040  AGATCT              
NspI         1658  GCATGT               [GA]CATG^[CT]
             1896  GCATGC              
PpuMI         551  AGGACCT              [GA]G^G[AT]CC[CT]
             3314  GGGACCC             
SapI         2845  GAAGAGC              GCTCTTC(1/4)
             1015  GCTCTTC             
XhoII        1739  AGATCC               [GA]^GATC[CT]
             2040  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApoI        AspHI       AvaII       
BanI        BanII       BbvCI       BbvI        BcgI        
BfaI        BglI        BmyI        BpmI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspHI       BspMI       BsrBI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstXI       Bsu36I      Cac8I       Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        Eco81I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NcoI        NdeII       NlaIII      NlaIV       
PleI        PstI        PvuII       RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
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