Restriction map for pF1KA0651
 (Linear) (Six-base) MAPSORT of: KIAA0651.seq  Check: 2516  from: 1  to: 3240



KIAA0651.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:21  ..

Acc65I G'GTAC_C

Cuts at:      0     280    3240 
   Size:        280    2960
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     297     601    1063    1820    3240 
   Size:        297     304     462     757    1420

  Fragments arranged by size: 

                1420     757     462     304     297
 
AleI CACnn'nnGTG

Cuts at:      0      74    3240 
   Size:         74    3166
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     973    1090    3240 
   Size:        973     117    2150

  Fragments arranged by size: 

                2150     973     117
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2563    2595    3240 
   Size:       2563      32     645

  Fragments arranged by size: 

                2563     645      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1116    1194    1524    1654    3069    3240 
   Size:       1116      78     330     130    1415     171

  Fragments arranged by size: 

                1415    1116     330     171     130      78
 
ApaI G_GGCC'C

Cuts at:      0    1602    1641    1666    2593    2910    3240 
   Size:       1602      39      25     927     317     330

  Fragments arranged by size: 

                1602     927     330     317      39      25
 
ApaLI G'TGCA_C

Cuts at:      0    1933    3240 
   Size:       1933    1307
 
AvaI C'yCGr_G

Cuts at:      0     284     324     350    1352    1634    1946    2321    2627 
   Size:        284      40      26    1002     282     312     375     306

Cuts at:   2627    2816    3240 
   Size:        189     424

  Fragments arranged by size: 

                1002     424     375     312     306     284     282     189
                  40      26
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     272     305    1411    1444    3240 
   Size:        272      33    1106      33    1796

  Fragments arranged by size: 

                1796    1106     272      33      33
 
BamHI G'GATC_C

Cuts at:      0     791    1967    2922    3240 
   Size:        791    1176     955     318

  Fragments arranged by size: 

                1176     955     791     318
 
BanI G'GyrC_C

Cuts at:      0     280     401    3240 
   Size:        280     121    2839

  Fragments arranged by size: 

                2839     280     121
 
BanII G_rGCy'C

Cuts at:      0     694    1139    1602    1641    1666    2593    2910    3079 
   Size:        694     445     463      39      25     927     317     169

Cuts at:   3079    3240 
   Size:        161

  Fragments arranged by size: 

                 927     694     463     445     317     169     161      39
                  25
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1732    3240 
   Size:       1732    1508
 
BbvCI CC'TCA_GC

Cuts at:      0    1162    2894    3240 
   Size:       1162    1732     346

  Fragments arranged by size: 

                1732    1162     346
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     198     231    3240 
   Size:        198      33    3009

  Fragments arranged by size: 

                3009     198      33
 
BclI T'GATC_A

Cuts at:      0    1901    3240 
   Size:       1901    1339
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1670    2665    2845    2905    3240 
   Size:       1670     995     180      60     335

  Fragments arranged by size: 

                1670     995     335     180      60
 
BglII A'GATC_T

Cuts at:      0    1613    3240 
   Size:       1613    1627
 
Bme1580I G_kGCm'C

Cuts at:      0     404    1201    1602    1641    1666    1937    2091    2593 
   Size:        404     797     401      39      25     271     154     502

Cuts at:   2593    2823    2910    3240 
   Size:        230      87     330

  Fragments arranged by size: 

                 797     502     404     401     330     271     230     154
                  87      39      25
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     219    1546    1642    3079    3240 
   Size:        219    1327      96    1437     161

  Fragments arranged by size: 

                1437    1327     219     161      96
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1609    1774    2806    2848    3240 
   Size:       1609     165    1032      42     392

  Fragments arranged by size: 

                1609    1032     392     165      42
 
Bpu10I CC'TnA_GC

Cuts at:      0    1162    2594    2894    3240 
   Size:       1162    1432     300     346

  Fragments arranged by size: 

                1432    1162     346     300
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1390    2239    2985    3240 
   Size:       1390     849     746     255

  Fragments arranged by size: 

                1390     849     746     255
 
BsaHI Gr'CG_yC

Cuts at:      0    1185    3240 
   Size:       1185    2055
 
BsaWI w'CCGG_w

Cuts at:      0     334    1379    1955    3240 
   Size:        334    1045     576    1285

  Fragments arranged by size: 

                1285    1045     576     334
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1267    1297    1543    1573    2596    2626    3240 
   Size:       1267      30     246      30    1023      30     614

  Fragments arranged by size: 

                1267    1023     614     246      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     285     699    1522    1525    2503    2743    3139    3211 
   Size:        285     414     823       3     978     240     396      72

Cuts at:   3211    3240 
   Size:         29

  Fragments arranged by size: 

                 978     823     414     396     285     240      72      29
                   3
 
BseYI C'CCAG_C

Cuts at:      0    2552    2650    2889    2986    3240 
   Size:       2552      98     239      97     254

  Fragments arranged by size: 

                2552     254     239      98      97
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1009    3240 
   Size:       1009    2231
 
BsiHKAI G_wGCw'C

Cuts at:      0    1139    1406    1937    1963    3240 
   Size:       1139     267     531      26    1277

  Fragments arranged by size: 

                1277    1139     531     267      26
 
BsiWI C'GTAC_G

Cuts at:      0    1863    3240 
   Size:       1863    1377
 
BsmI GAATG_Cn'

Cuts at:      0    1491    3185    3240 
   Size:       1491    1694      55

  Fragments arranged by size: 

                1694    1491      55
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3193    3240 
   Size:       3193      47
 
Bsp1286I G_dGCh'C

Cuts at:      0     404     694    1139    1201    1406    1602    1641    1666 
   Size:        404     290     445      62     205     196      39      25

Cuts at:   1666    1937    1963    2091    2593    2823    2910    3079    3240 
   Size:        271      26     128     502     230      87     169     161

  Fragments arranged by size: 

                 502     445     404     290     271     230     205     196
                 169     161     128      87      62      39      26      25
            
 
BspEI T'CCGG_A

Cuts at:      0    1379    3240 
   Size:       1379    1861
 
BspHI T'CATG_A

Cuts at:      0    1049    3240 
   Size:       1049    2191
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     502    2057    2291    3240 
   Size:        502    1555     234     949

  Fragments arranged by size: 

                1555     949     502     234
 
BsrBI CCG'CTC

Cuts at:      0     639     707    2207    2238    2598    3087    3240 
   Size:        639      68    1500      31     360     489     153

  Fragments arranged by size: 

                1500     639     489     360     153      68      31
 
BsrDI GCAATG_nn'

Cuts at:      0     504     746    1871    3240 
   Size:        504     242    1125    1369

  Fragments arranged by size: 

                1369    1125     504     242
 
BsrFI r'CCGG_y

Cuts at:      0    2744    3240 
   Size:       2744     496
 
BssSI C'ACGA_G

Cuts at:      0    2228    2364    3164    3240 
   Size:       2228     136     800      76

  Fragments arranged by size: 

                2228     800     136      76
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1989    2953    3240 
   Size:       1989     964     287

  Fragments arranged by size: 

                1989     964     287
 
BstBI TT'CG_AA

Cuts at:      0     615    3240 
   Size:        615    2625
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1536    2691    3240 
   Size:       1536    1155     549

  Fragments arranged by size: 

                1536    1155     549
 
BstYI r'GATC_y

Cuts at:      0      33     791    1613    1967    2922    3009    3240 
   Size:         33     758     822     354     955      87     231

  Fragments arranged by size: 

                 955     822     758     354     231      87      33
 
Bsu36I CC'TnA_GG

Cuts at:      0     244    2585    3240 
   Size:        244    2341     655

  Fragments arranged by size: 

                2341     655     244
 
BtgI C'CryG_G

Cuts at:      0     699     842    1497    2684    3240 
   Size:        699     143     655    1187     556

  Fragments arranged by size: 

                1187     699     655     556     143
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1213    2966    3240 
   Size:       1213    1753     274

  Fragments arranged by size: 

                1753    1213     274
 
BtsI GCAGTG_nn'

Cuts at:      0    2172    2378    2821    3108    3240 
   Size:       2172     206     443     287     132

  Fragments arranged by size: 

                2172     443     287     206     132
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     729     764    3240 
   Size:        729      35    2476

  Fragments arranged by size: 

                2476     729      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1032    3240 
   Size:       1032    2208
 
EaeI y'GGCC_r

Cuts at:      0     140     640     650     702    1671    2861    3240 
   Size:        140     500      10      52     969    1190     379

  Fragments arranged by size: 

                1190     969     500     379     140      52      10
 
EarI CTCTTCn'nnn_

Cuts at:      0      29     412     617     857    1066    1076    2147    2837 
   Size:         29     383     205     240     209      10    1071     690

Cuts at:   2837    3077    3240 
   Size:        240     163

  Fragments arranged by size: 

                1071     690     383     240     240     209     205     163
                  29      10
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3140    3240 
   Size:       3140     100
 
EcoICRI GAG'CTC

Cuts at:      0    1137    3240 
   Size:       1137    2103
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     297     601    1063    1609    1774    1820    2806    2848 
   Size:        297     304     462     546     165      46     986      42

Cuts at:   2848    3240 
   Size:        392

  Fragments arranged by size: 

                 986     546     462     392     304     297     165      46
                  42
 
EcoO109I rG'GnC_Cy

Cuts at:      0       8      19    1113    1191    1264    1521    1598    1662 
   Size:          8      11    1094      78      73     257      77      64

Cuts at:   1662    1663    2196    2589    2590    2898    3240 
   Size:          1     533     393       1     308     342

  Fragments arranged by size: 

                1094     533     393     342     308     257      78      77
                  73      64      11       8       1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1683    1830    3240 
   Size:       1683     147    1410

  Fragments arranged by size: 

                1683    1410     147
 
FspI TGC'GCA

Cuts at:      0    1993    3240 
   Size:       1993    1247
 
FspAI rTGC'GCAy

Cuts at:      0    1993    3240 
   Size:       1993    1247
 
HaeII r_GCGC'y

Cuts at:      0    1518    1743    2742    3240 
   Size:       1518     225     999     498

  Fragments arranged by size: 

                1518     999     498     225
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1543    1575    2099    2131    2425    2457    3240 
   Size:       1543      32     524      32     294      32     783

  Fragments arranged by size: 

                1543     783     524     294      32      32      32
 
KpnI G_GTAC'C

Cuts at:      0     284    3240 
   Size:        284    2956
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     689    3240 
   Size:        689    2551
 
MslI CAynn'nnrTG

Cuts at:      0      74     148     436     761    1072    2566    3240 
   Size:         74      74     288     325     311    1494     674

  Fragments arranged by size: 

                1494     674     325     311     288      74      74
 
MspA1I CmG'CkG

Cuts at:      0     264     518     922    1587    2354    2472    2545    2706 
   Size:        264     254     404     665     767     118      73     161

Cuts at:   2706    2859    2893    3240 
   Size:        153      34     347

  Fragments arranged by size: 

                 767     665     404     347     264     254     161     153
                 118      73      34
 
NaeI GCC'GGC

Cuts at:      0    2746    3240 
   Size:       2746     494
 
NgoMIV G'CCGG_C

Cuts at:      0    2744    3240 
   Size:       2744     496
 
NspI r_CATG'y

Cuts at:      0     153     825    2000    2716    3240 
   Size:        153     672    1175     716     524

  Fragments arranged by size: 

                1175     716     672     524     153
 
PasI CC'CwG_GG

Cuts at:      0     777    2192    2868    2903    3240 
   Size:        777    1415     676      35     337

  Fragments arranged by size: 

                1415     777     676     337      35
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     121    1233    1654    3240 
   Size:        121    1112     421    1586

  Fragments arranged by size: 

                1586    1112     421     121
 
PfoI T'CCnGG_A

Cuts at:      0     632    1790    2798    3191    3240 
   Size:        632    1158    1008     393      49

  Fragments arranged by size: 

                1158    1008     632     393      49
 
PpuMI rG'GwC_Cy

Cuts at:      0    1264    1521    3240 
   Size:       1264     257    1719

  Fragments arranged by size: 

                1719    1264     257
 
PspOMI G'GGCC_C

Cuts at:      0    1598    1637    1662    2589    2906    3240 
   Size:       1598      39      25     927     317     334

  Fragments arranged by size: 

                1598     927     334     317      39      25
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2681    2713    3240 
   Size:       2681      32     527

  Fragments arranged by size: 

                2681     527      32
 
PstI C_TGCA'G

Cuts at:      0      33     259     962    1309    1490    1610    1853    2249 
   Size:         33     226     703     347     181     120     243     396

Cuts at:   2249    2428    2705    2726    3240 
   Size:        179     277      21     514

  Fragments arranged by size: 

                 703     514     396     347     277     243     226     181
                 179     120      33      21
 
PvuII CAG'CTG

Cuts at:      0     264     922    1587    2472    2545    2859    2893    3240 
   Size:        264     658     665     885      73     314      34     347

  Fragments arranged by size: 

                 885     665     658     347     314     264      73      34
            
 
SacI G_AGCT'C

Cuts at:      0    1139    3240 
   Size:       1139    2101
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1076    3077    3240 
   Size:       1076    2001     163

  Fragments arranged by size: 

                2001    1076     163
 
SfcI C'TryA_G

Cuts at:      0      29     255     873     958    1305    1486    1606    1849 
   Size:         29     226     618      85     347     181     120     243

Cuts at:   1849    2245    2424    2536    2701    2722    3224    3240 
   Size:        396     179     112     165      21     502      16

  Fragments arranged by size: 

                 618     502     396     347     243     226     181     179
                 165     120     112      85      29      21      16
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1670    2905    3240 
   Size:       1670    1235     335

  Fragments arranged by size: 

                1670    1235     335
 
SmaI CCC'GGG

Cuts at:      0     286     352    1948    2818    3240 
   Size:        286      66    1596     870     422

  Fragments arranged by size: 

                1596     870     422     286      66
 
SmlI C'TyrA_G

Cuts at:      0    1405    2218    2964    3240 
   Size:       1405     813     746     276

  Fragments arranged by size: 

                1405     813     746     276
 
SphI G_CATG'C

Cuts at:      0     153    3240 
   Size:        153    3087
 
SrfI GCCC'GGGC

Cuts at:      0    2818    3240 
   Size:       2818     422
 
StuI AGG'CCT

Cuts at:      0    1324    3240 
   Size:       1324    1916
 
StyI C'CwwG_G

Cuts at:      0      12     377     470     545    2339    3240 
   Size:         12     365      93      75    1794     901

  Fragments arranged by size: 

                1794     901     365      93      75      12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1225    2893    3068    3240 
   Size:       1225    1668     175     172

  Fragments arranged by size: 

                1668    1225     175     172
 
TatI w'GTAC_w

Cuts at:      0    1776    1808    2960    3240 
   Size:       1776      32    1152     280

  Fragments arranged by size: 

                1776    1152     280      32
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1267    1299    1927    1959    2596    2628    3240 
   Size:       1267      32     628      32     637      32     612

  Fragments arranged by size: 

                1267     637     628     612      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     142    2262    3240 
   Size:        142    2120     978

  Fragments arranged by size: 

                2120     978     142
 
XmaI C'CCGG_G

Cuts at:      0     284     350    1946    2816    3240 
   Size:        284      66    1596     870     424

  Fragments arranged by size: 

                1596     870     424     284      66
 

 Enzymes that do cut:

   Acc65I     AcuI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AvaI     BaeI    BamHI     BanI    BanII     BbsI    BbvCI    BciVI
     BclI     BglI    BglII Bme1580I     BmrI     BpmI   Bpu10I    BpuEI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI    CspCI   DraIII     EaeI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I     FalI     FspI    FspAI    HaeII    Hin4I     KpnI
     MmeI     MslI   MspA1I     NaeI   NgoMIV     NspI     PasI    PflMI
     PfoI    PpuMI   PspOMI     PsrI     PstI    PvuII     SacI     SapI
     SfcI     SfiI     SmaI     SmlI     SphI     SrfI     StuI     StyI
    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     ApoI     AscI     AseI    AsiSI    AvrII     BbeI     BcgI
    BfrBI     BlpI    BmgBI     BmtI     BplI     BsaI    BsaAI    BsaBI
    BsiEI    BsrGI   BssHII   BstEII  BstZ17I     ClaI     DraI     DrdI
     EagI    EcoNI    EcoRI    EcoRV     FseI   HincII  HindIII     HpaI
     KasI     MfeI     MluI     MscI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SspI     SwaI  Tth111I     XbaI     XhoI     XmnI     ZraI
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