################################## # NO CUT ENZYMES ( 33 enzymes) # ################################## Acc65I AclI AgeI AhdI AscI AseI AsnI Asp718I AspEI AvrII BlnI BssHII CpoI DraI DrdI Eam1105I HpaI KpnI NheI NotI PacI PinAI PmeI PshBI Psp1406I PvuI RsrII SalI SexAI SgrAI SpeI SspI SwaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccI 7638 GTATAC GT^[AC][GT]AC AccIII 8480 TCCGGA T^CCGGA AflII 7119 CTTAAG C^TTAAG BbrPI 403 CACGTG CAC^GTG BcgI 205 GCAGCCCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 4215 GTATCC GTATCC(6/5) BclI 8912 TGATCA T^GATCA BfrI 7119 CTTAAG C^TTAAG BseAI 8480 TCCGGA T^CCGGA BsiWI 639 CGTACG C^GTACG BspDI 7351 ATCGAT AT^CGAT BspEI 8480 TCCGGA T^CCGGA BspHI 8909 TCATGA T^CATGA BssSI 222 CACGAG CACGAG(-5/-1) Bst1107I 7638 GTATAC GTA^TAC BstBI 4007 TTCGAA TT^CGAA BstZ17I 7638 GTATAC GTA^TAC ClaI 7351 ATCGAT AT^CGAT EcoT22I 7347 ATGCAT ATGCA^T FbaI 8912 TGATCA T^GATCA FseI 8535 GGCCGGCC GGCCGG^CC HindIII 8917 AAGCTT A^AGCTT MluI 4943 ACGCGT A^CGCGT MroI 8480 TCCGGA T^CCGGA NdeI 7907 CATATG CA^TATG NruI 2845 TCGCGA TCG^CGA NsiI 7347 ATGCAT ATGCA^T NspV 4007 TTCGAA TT^CGAA PmaCI 403 CACGTG CAC^GTG PmlI 403 CACGTG CAC^GTG RcaI 8909 TCATGA T^CATGA SapI 3177 GAAGAGC GCTCTTC(1/4) ScaI 8927 AGTACT AGT^ACT SfuI 4007 TTCGAA TT^CGAA SnaBI 5499 TACGTA TAC^GTA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AatII 3331 GACGTC GACGT^C 8152 GACGTC Aor51HI 2920 AGCGCT AGC^GCT 3481 AGCGCT ApaI 5916 GGGCCC GGGCC^C 8705 GGGCCC AspI 924 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 5017 GACAGGGTC BbvCI 5588 CCTCAGC CCTCAGC(-5/-2) 2754 GCTGAGG BglII 4682 AGATCT A^GATCT 6038 AGATCT BsaAI 403 CACGTG [CT]AC^GT[GA] 5499 TACGTA BsaWI 3040 ACCGGA [AT]^CCGG[AT] 8480 TCCGGA BsmI 7725 GAATGC GAATGC(1/-1) 2135 GCATTC BspLU11I 718 ACATGT A^CATGT 4226 ACATGT Eco47III 2920 AGCGCT AGC^GCT 3481 AGCGCT HincII 4753 GTCAAC GT[CT]^[GA]AC 6630 GTCAAC HindII 4753 GTCAAC GT[CT]^[GA]AC 6630 GTCAAC KspI 4936 CCGCGG CCGC^GG 7493 CCGCGG MfeI 2113 CAATTG C^AATTG 3995 CAATTG MunI 2113 CAATTG C^AATTG 3995 CAATTG PaeR7I 6292 CTCGAG C^TCGAG 8629 CTCGAG PflFI 924 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 5017 GACAGGGTC PshAI 678 GACGATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5184 GACATCCGTC SacII 4936 CCGCGG CCGC^GG 7493 CCGCGG SbfI 5196 CCTGCAGG CCTGCA^GG 8229 CCTGCAGG SfiI 969 GGCCACCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 8526 GGCCGTGCGGGCC SmaI 2939 CCCGGG CCC^GGG 4774 CCCGGG SphI 2602 GCATGC GCATG^C 7345 GCATGC Sse8387I 5196 CCTGCAGG CCTGCA^GG 8229 CCTGCAGG Tth111I 924 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 5017 GACAGGGTC XbaI 2089 TCTAGA T^CTAGA 2404 TCTAGA XhoI 6292 CTCGAG C^TCGAG 8629 CTCGAG XmaCI 2939 CCCGGG C^CCGGG 4774 CCCGGG XmaI 2939 CCCGGG C^CCGGG 4774 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 160 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaLI ApoI Asp700I AspHI AvaI AvaII AviII BalI BamHI BanI BanII BbeI BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI