Restriction map for pF1KA0606
 (Linear) (Six-base) MAPSORT of: KIAA0606.seq  Check: 1742  from: 1  to: 3615



KIAA0606.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3218    3615 
   Size:       3218     397
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1591    2021    2174    2431    3199    3615 
   Size:       1591     430     153     257     768     416

  Fragments arranged by size: 

                1591     768     430     416     257     153
 
AfeI AGC'GCT

Cuts at:      0    1091    2712    3007    3615 
   Size:       1091    1621     295     608

  Fragments arranged by size: 

                1621    1091     608     295
 
AflII C'TTAA_G

Cuts at:      0     336    3615 
   Size:        336    3279
 
AflIII A'CryG_T

Cuts at:      0     623     894    3615 
   Size:        623     271    2721

  Fragments arranged by size: 

                2721     623     271
 
AhdI GACnn_n'nnGTC

Cuts at:      0     145    2601    3615 
   Size:        145    2456    1014

  Fragments arranged by size: 

                2456    1014     145
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     655    1732    3615 
   Size:        655    1077    1883

  Fragments arranged by size: 

                1883    1077     655
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2750    3210    3615 
   Size:       2750     460     405

  Fragments arranged by size: 

                2750     460     405
 
ApaLI G'TGCA_C

Cuts at:      0    2130    3615 
   Size:       2130    1485
 
ApoI r'AATT_y

Cuts at:      0     775    3615 
   Size:        775    2840
 
AvrII C'CTAG_G

Cuts at:      0    2574    3615 
   Size:       2574    1041
 
BamHI G'GATC_C

Cuts at:      0    2289    2299    3438    3615 
   Size:       2289      10    1139     177

  Fragments arranged by size: 

                2289    1139     177      10
 
BanI G'GyrC_C

Cuts at:      0    2112    2251    2517    3219    3320    3615 
   Size:       2112     139     266     702     101     295

  Fragments arranged by size: 

                2112     702     295     266     139     101
 
BanII G_rGCy'C

Cuts at:      0    1394    1974    2765    2906    2957    2970    3119    3615 
   Size:       1394     580     791     141      51      13     149     496

  Fragments arranged by size: 

                1394     791     580     496     149     141      51      13
            
 
BbeI G_GCGC'C

Cuts at:      0    3324    3615 
   Size:       3324     291
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    3123    3615 
   Size:       3123     492
 
BbvCI CC'TCA_GC

Cuts at:      0    3545    3615 
   Size:       3545      70
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2024    2058    3615 
   Size:       2024      34    1557

  Fragments arranged by size: 

                2024    1557      34
 
BclI T'GATC_A

Cuts at:      0    1893    1941    2828    3447    3615 
   Size:       1893      48     887     619     168

  Fragments arranged by size: 

                1893     887     619     168      48
 
BfrBI ATG'CAT

Cuts at:      0     210    3615 
   Size:        210    3405
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1335    2776    3615 
   Size:       1335    1441     839

  Fragments arranged by size: 

                1441    1335     839
 
BglII A'GATC_T

Cuts at:      0    2380    3615 
   Size:       2380    1235
 
BlpI GC'TnA_GC

Cuts at:      0    2764    3431    3615 
   Size:       2764     667     184

  Fragments arranged by size: 

                2764     667     184
 
Bme1580I G_kGCm'C

Cuts at:      0    1553    2117    2134    2642    3615 
   Size:       1553     564      17     508     973

  Fragments arranged by size: 

                1553     973     564     508      17
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1129    2504    2786    2990    3615 
   Size:       1129    1375     282     204     625

  Fragments arranged by size: 

                1375    1129     625     282     204
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1454    1660    2110    2853    3428    3535    3615 
   Size:       1454     206     450     743     575     107      80

  Fragments arranged by size: 

                1454     743     575     450     206     107      80
 
Bpu10I CC'TnA_GC

Cuts at:      0    1798    2971    3545    3615 
   Size:       1798    1173     574      70

  Fragments arranged by size: 

                1798    1173     574      70
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1408    3615 
   Size:       1408    2207
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     844    2090    3615 
   Size:        844    1246    1525

  Fragments arranged by size: 

                1525    1246     844
 
BsaAI yAC'GTr

Cuts at:      0    2532    3615 
   Size:       2532    1083
 
BsaHI Gr'CG_yC

Cuts at:      0     141    3128    3321    3615 
   Size:        141    2987     193     294

  Fragments arranged by size: 

                2987     294     193     141
 
BsaWI w'CCGG_w

Cuts at:      0     199    1289    1966    2099    3615 
   Size:        199    1090     677     133    1516

  Fragments arranged by size: 

                1516    1090     677     199     133
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1977    2007    3615 
   Size:       1977      30    1608

  Fragments arranged by size: 

                1977    1608      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     270    2626    3127    3493    3615 
   Size:        270    2356     501     366     122

  Fragments arranged by size: 

                2356     501     366     270     122
 
BseYI C'CCAG_C

Cuts at:      0     371    1974    2233    2853    3615 
   Size:        371    1603     259     620     762

  Fragments arranged by size: 

                1603     762     620     371     259
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2554    2743    3615 
   Size:       2554     189     872

  Fragments arranged by size: 

                2554     872     189
 
BsiHKAI G_wGCw'C

Cuts at:      0    1394    2134    2546    2765    2906    3615 
   Size:       1394     740     412     219     141     709

  Fragments arranged by size: 

                1394     740     709     412     219     141
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1952    3615 
   Size:       1952    1663
 
Bsp1286I G_dGCh'C

Cuts at:      0    1394    1553    1974    2117    2134    2546    2642    2765 
   Size:       1394     159     421     143      17     412      96     123

Cuts at:   2765    2906    2957    2970    3119    3615 
   Size:        141      51      13     149     496

  Fragments arranged by size: 

                1394     496     421     412     159     149     143     141
                 123      96      51      17      13
 
BspEI T'CCGG_A

Cuts at:      0    1289    1966    3615 
   Size:       1289     677    1649

  Fragments arranged by size: 

                1649    1289     677
 
BspHI T'CATG_A

Cuts at:      0    1700    2390    3494    3615 
   Size:       1700     690    1104     121

  Fragments arranged by size: 

                1700    1104     690     121
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     172    1847    3218    3615 
   Size:        172    1675    1371     397

  Fragments arranged by size: 

                1675    1371     397     172
 
BsrDI GCAATG_nn'

Cuts at:      0     639    3615 
   Size:        639    2976
 
BsrFI r'CCGG_y

Cuts at:      0    2770    3615 
   Size:       2770     845
 
BssSI C'ACGA_G

Cuts at:      0     396    3615 
   Size:        396    3219
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     216    2541    3210    3615 
   Size:        216    2325     669     405

  Fragments arranged by size: 

                2325     669     405     216
 
BstBI TT'CG_AA

Cuts at:      0    3399    3615 
   Size:       3399     216
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1982    3615 
   Size:       1982    1633
 
BstYI r'GATC_y

Cuts at:      0     900    1580    2289    2299    2380    3438    3615 
   Size:        900     680     709      10      81    1058     177

  Fragments arranged by size: 

                1058     900     709     680     177      81      10
 
Bsu36I CC'TnA_GG

Cuts at:      0     478     856    3615 
   Size:        478     378    2759

  Fragments arranged by size: 

                2759     478     378
 
BtgI C'CryG_G

Cuts at:      0    2618    3565    3615 
   Size:       2618     947      50

  Fragments arranged by size: 

                2618     947      50
 
BtsI GCAGTG_nn'

Cuts at:      0    2904    3171    3615 
   Size:       2904     267     444

  Fragments arranged by size: 

                2904     444     267
 
DraI TTT'AAA

Cuts at:      0     464    3615 
   Size:        464    3151
 
DraIII CAC_nnn'GTG

Cuts at:      0    1725    3369    3615 
   Size:       1725    1644     246

  Fragments arranged by size: 

                1725    1644     246
 
EaeI y'GGCC_r

Cuts at:      0    1236    3615 
   Size:       1236    2379
 
EarI CTCTTCn'nnn_

Cuts at:      0     436     538    1107    1481    2150    2399    2407    3401 
   Size:        436     102     569     374     669     249       8     994

Cuts at:   3401    3470    3615 
   Size:         69     145

  Fragments arranged by size: 

                 994     669     569     436     374     249     145     102
                  69       8
 
EcoICRI GAG'CTC

Cuts at:      0    1392    2763    2904    3615 
   Size:       1392    1371     141     711

  Fragments arranged by size: 

                1392    1371     711     141
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1454    1591    1660    2021    2110    2174    2431    2853 
   Size:       1454     137      69     361      89      64     257     422

Cuts at:   2853    3199    3428    3535    3615 
   Size:        346     229     107      80

  Fragments arranged by size: 

                1454     422     361     346     257     229     137     107
                  89      80      69      64
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1952    3214    3615 
   Size:       1952    1262     401

  Fragments arranged by size: 

                1952    1262     401
 
EcoO109I rG'GnC_Cy

Cuts at:      0     161    3615 
   Size:        161    3454
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     317    3312    3615 
   Size:        317    2995     303

  Fragments arranged by size: 

                2995     317     303
 
FspI TGC'GCA

Cuts at:      0    2632    3615 
   Size:       2632     983
 
HaeII r_GCGC'y

Cuts at:      0    1093    2714    2771    3009    3051    3065    3080    3260 
   Size:       1093    1621      57     238      42      14      15     180

Cuts at:   3260    3324    3615 
   Size:         64     291

  Fragments arranged by size: 

                1621    1093     291     238     180      64      57      42
                  15      14
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     131     163    2372    2404    2587    2619    2820    2827 
   Size:        131      32    2209      32     183      32     201       7

Cuts at:   2827    2852    2859    2920    2952    3615 
   Size:         25       7      61      32     663

  Fragments arranged by size: 

                2209     663     201     183     131      61      32      32
                  32      32      25       7       7
 
HincII GTy'rAC

Cuts at:      0    1559    2939    3615 
   Size:       1559    1380     676

  Fragments arranged by size: 

                1559    1380     676
 
HindIII A'AGCT_T

Cuts at:      0     988    2003    2242    3615 
   Size:        988    1015     239    1373

  Fragments arranged by size: 

                1373    1015     988     239
 
HpaI GTT'AAC

Cuts at:      0    1559    3615 
   Size:       1559    2056
 
KasI G'GCGC_C

Cuts at:      0    3320    3615 
   Size:       3320     295
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     628     642     674     710    1763    3059    3110    3615 
   Size:        628      14      32      36    1053    1296      51     505

  Fragments arranged by size: 

                1296    1053     628     505      51      36      32      14
            
 
MscI TGG'CCA

Cuts at:      0    1238    3615 
   Size:       1238    2377
 
MslI CAynn'nnrTG

Cuts at:      0    2209    2510    3615 
   Size:       2209     301    1105

  Fragments arranged by size: 

                2209    1105     301
 
MspA1I CmG'CkG

Cuts at:      0     502    1928    1978    2257    2367    2876    3561    3567 
   Size:        502    1426      50     279     110     509     685       6

Cuts at:   3567    3579    3588    3615 
   Size:         12       9      27

  Fragments arranged by size: 

                1426     685     509     502     279     110      50      27
                  12       9       6
 
NarI GG'CG_CC

Cuts at:      0    3321    3615 
   Size:       3321     294
 
NsiI A_TGCA'T

Cuts at:      0     212    3615 
   Size:        212    3403
 
NspI r_CATG'y

Cuts at:      0     214     898     961    1719    3015    3615 
   Size:        214     684      63     758    1296     600

  Fragments arranged by size: 

                1296     758     684     600     214      63
 
PciI A'CATG_T

Cuts at:      0     894    3615 
   Size:        894    2721
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     226    3615 
   Size:        226    3389
 
PfoI T'CCnGG_A

Cuts at:      0    2292    3615 
   Size:       2292    1323
 
PmlI CAC'GTG

Cuts at:      0    2532    3615 
   Size:       2532    1083
 
PpuMI rG'GwC_Cy

Cuts at:      0     161    3615 
   Size:        161    3454
 
PstI C_TGCA'G

Cuts at:      0     598    1856    3178    3615 
   Size:        598    1258    1322     437

  Fragments arranged by size: 

                1322    1258     598     437
 
PvuII CAG'CTG

Cuts at:      0     502    1978    3561    3579    3588    3615 
   Size:        502    1476    1583      18       9      27

  Fragments arranged by size: 

                1583    1476     502      27      18       9
 
SacI G_AGCT'C

Cuts at:      0    1394    2765    2906    3615 
   Size:       1394    1371     141     709

  Fragments arranged by size: 

                1394    1371     709     141
 
SacII CC_GC'GG

Cuts at:      0    3568    3615 
   Size:       3568      47
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2150    2399    3615 
   Size:       2150     249    1216

  Fragments arranged by size: 

                2150    1216     249
 
SfcI C'TryA_G

Cuts at:      0     435     594     862     952    1852    1952    3174    3383 
   Size:        435     159     268      90     900     100    1222     209

Cuts at:   3383    3615 
   Size:        232

  Fragments arranged by size: 

                1222     900     435     268     232     209     159     100
                  90
 
SfoI GGC'GCC

Cuts at:      0    3322    3615 
   Size:       3322     293
 
SgrAI Cr'CCGG_yG

Cuts at:      0    2770    3615 
   Size:       2770     845
 
SmlI C'TyrA_G

Cuts at:      0     336    1387    3615 
   Size:        336    1051    2228

  Fragments arranged by size: 

                2228    1051     336
 
SphI G_CATG'C

Cuts at:      0    1719    3015    3615 
   Size:       1719    1296     600

  Fragments arranged by size: 

                1719    1296     600
 
StyI C'CwwG_G

Cuts at:      0     359    2574    3185    3615 
   Size:        359    2215     611     430

  Fragments arranged by size: 

                2215     611     430     359
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2805    3615 
   Size:       2805     810
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     382     414     830     862    2521    2553    3615 
   Size:        382      32     416      32    1659      32    1062

  Fragments arranged by size: 

                1659    1062     416     382      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2472    3615 
   Size:       2472    1143
 
XbaI T'CTAG_A

Cuts at:      0     999    3615 
   Size:        999    2616
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     128    2484    2877    3615 
   Size:        128    2356     393     738

  Fragments arranged by size: 

                2356     738     393     128
 
XmnI GAAnn'nnTTC

Cuts at:      0      71    1115    1463    2053    3615 
   Size:         71    1044     348     590    1562

  Fragments arranged by size: 

                1562    1044     590     348      71
 

 Enzymes that do cut:

     AarI     AcuI     AfeI    AflII   AflIII     AhdI     AlfI    AlwNI
    ApaLI     ApoI    AvrII    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI
    BstYI   Bsu36I     BtgI     BtsI     DraI   DraIII     EaeI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     MmeI     MscI     MslI   MspA1I
     NarI     NsiI     NspI     PciI    PflMI     PfoI     PmlI    PpuMI
     PstI    PvuII     SacI    SacII     SapI     SfcI     SfoI    SgrAI
     SmlI     SphI     StyI    TaqII     TstI  Tth111I     XbaI     XcmI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AgeI     AleI     AloI     ApaI
     AscI     AseI    AsiSI     AvaI     BaeI    BciVI    BmgBI     BmtI
     BplI    BsaBI    BsiEI    BsiWI     BsmI    BsrBI    BsrGI   BssHII
   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DrdI     EagI     EciI
    EcoRI    EcoRV     FseI    FspAI     KpnI     MfeI     MluI     NaeI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SgfI
     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI     TatI
     XhoI     XmaI     ZraI
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