Restriction map for pF1KA0598
 (Linear) (Six-base) MAPSORT of: KIAA0598.seq  Check: 431  from: 1  to: 1683



KIAA0598.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:21  ..

AccI GT'mk_AC

Cuts at:      0    1316    1683 
   Size:       1316     367
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206    1683 
   Size:        206    1477
 
AfeI AGC'GCT

Cuts at:      0     670     880    1683 
   Size:        670     210     803

  Fragments arranged by size: 

                 803     670     210
 
AflII C'TTAA_G

Cuts at:      0     526    1500    1683 
   Size:        526     974     183

  Fragments arranged by size: 

                 974     526     183
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1162    1683 
   Size:       1162     521
 
AleI CACnn'nnGTG

Cuts at:      0     864    1683 
   Size:        864     819
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1485    1683 
   Size:       1485     198
 
ApaI G_GGCC'C

Cuts at:      0      79     303    1499    1580    1683 
   Size:         79     224    1196      81     103

  Fragments arranged by size: 

                1196     224     103      81      79
 
ApoI r'AATT_y

Cuts at:      0     125     568    1683 
   Size:        125     443    1115

  Fragments arranged by size: 

                1115     443     125
 
AseI AT'TA_AT

Cuts at:      0      14     460    1683 
   Size:         14     446    1223

  Fragments arranged by size: 

                1223     446      14
 
AvaI C'yCGr_G

Cuts at:      0     612    1558    1683 
   Size:        612     946     125

  Fragments arranged by size: 

                 946     612     125
 
AvrII C'CTAG_G

Cuts at:      0    1491    1683 
   Size:       1491     192
 
BanII G_rGCy'C

Cuts at:      0      79     303     592    1367    1499    1537    1580    1683 
   Size:         79     224     289     775     132      38      43     103

  Fragments arranged by size: 

                 775     289     224     132     103      79      43      38
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     527    1467    1681    1683 
   Size:        527     940     214       2

  Fragments arranged by size: 

                 940     527     214       2
 
BfrBI ATG'CAT

Cuts at:      0    1444    1683 
   Size:       1444     239
 
BglI GCCn_nnn'nGGC

Cuts at:      0      74    1350    1584    1683 
   Size:         74    1276     234      99

  Fragments arranged by size: 

                1276     234      99      74
 
BlpI GC'TnA_GC

Cuts at:      0    1393    1683 
   Size:       1393     290
 
Bme1580I G_kGCm'C

Cuts at:      0      79     103     303    1499    1580    1683 
   Size:         79      24     200    1196      81     103

  Fragments arranged by size: 

                1196     200     103      81      79      24
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     210    1683 
   Size:        210    1473
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     627    1683 
   Size:        627    1056
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1339    1683 
   Size:       1339     344
 
Bpu10I CC'TnA_GC

Cuts at:      0    1171    1683 
   Size:       1171     512
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     908    1683 
   Size:        908     775
 
BsaAI yAC'GTr

Cuts at:      0     107     654    1683 
   Size:        107     547    1029

  Fragments arranged by size: 

                1029     547     107
 
BsaHI Gr'CG_yC

Cuts at:      0     689    1554    1683 
   Size:        689     865     129

  Fragments arranged by size: 

                 865     689     129
 
BsaWI w'CCGG_w

Cuts at:      0    1565    1683 
   Size:       1565     118
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1177    1207    1683 
   Size:       1177      30     476

  Fragments arranged by size: 

                1177     476      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     619    1683 
   Size:        619    1064
 
BseYI C'CCAG_C

Cuts at:      0     349     358    1683 
   Size:        349       9    1325

  Fragments arranged by size: 

                1325     349       9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     808    1683 
   Size:        808     875
 
BsiHKAI G_wGCw'C

Cuts at:      0     659    1683 
   Size:        659    1024
 
Bsp1286I G_dGCh'C

Cuts at:      0      79     103     303     592     659    1367    1499    1537 
   Size:         79      24     200     289      67     708     132      38

Cuts at:   1537    1580    1683 
   Size:         43     103

  Fragments arranged by size: 

                 708     289     200     132     103      79      67      43
                  38      24
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      48    1635    1683 
   Size:         48    1587      48

  Fragments arranged by size: 

                1587      48      48
 
BsrBI CCG'CTC

Cuts at:      0     677    1683 
   Size:        677    1006
 
BssHII G'CGCG_C

Cuts at:      0    1305    1683 
   Size:       1305     378
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      17     540    1546    1683 
   Size:         17     523    1006     137

  Fragments arranged by size: 

                1006     523     137      17
 
BstBI TT'CG_AA

Cuts at:      0    1222    1683 
   Size:       1222     461
 
BstEII G'GTnAC_C

Cuts at:      0      88    1683 
   Size:         88    1595
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     366     866    1683 
   Size:        366     500     817

  Fragments arranged by size: 

                 817     500     366
 
BstYI r'GATC_y

Cuts at:      0     968    1683 
   Size:        968     715
 
Bsu36I CC'TnA_GG

Cuts at:      0     830    1683 
   Size:        830     853
 
BtgI C'CryG_G

Cuts at:      0     926    1232    1683 
   Size:        926     306     451

  Fragments arranged by size: 

                 926     451     306
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     249    1683 
   Size:        249    1434
 
BtsI GCAGTG_nn'

Cuts at:      0       6    1264    1300    1683 
   Size:          6    1258      36     383

  Fragments arranged by size: 

                1258     383      36       6
 
DraIII CAC_nnn'GTG

Cuts at:      0    1055    1683 
   Size:       1055     628
 
EaeI y'GGCC_r

Cuts at:      0     956    1683 
   Size:        956     727
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     733     834     883    1683 
   Size:        733     101      49     800

  Fragments arranged by size: 

                 800     733     101      49
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206    1339    1683 
   Size:        206    1133     344

  Fragments arranged by size: 

                1133     344     206
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     697    1683 
   Size:        697     986
 
EcoO109I rG'GnC_Cy

Cuts at:      0      75      76     299    1176    1495    1496    1576    1622 
   Size:         75       1     223     877     319       1      80      46

Cuts at:   1622    1647    1683 
   Size:         25      36

  Fragments arranged by size: 

                 877     319     223      80      75      46      36      25
                   1       1
 
FspI TGC'GCA

Cuts at:      0     697    1683 
   Size:        697     986
 
HaeII r_GCGC'y

Cuts at:      0     672     882    1683 
   Size:        672     210     801

  Fragments arranged by size: 

                 801     672     210
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     227     259    1148    1180    1429    1461    1683 
   Size:        227      32     889      32     249      32     222

  Fragments arranged by size: 

                 889     249     227     222      32      32      32
 
HincII GTy'rAC

Cuts at:      0      63    1683 
   Size:         63    1620
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1472    1683 
   Size:       1472     211
 
MslI CAynn'nnrTG

Cuts at:      0     864    1345    1683 
   Size:        864     481     338

  Fragments arranged by size: 

                 864     481     338
 
MspA1I CmG'CkG

Cuts at:      0      30    1234    1272    1683 
   Size:         30    1204      38     411

  Fragments arranged by size: 

                1204     411      38      30
 
NdeI CA'TA_TG

Cuts at:      0     545    1683 
   Size:        545    1138
 
NsiI A_TGCA'T

Cuts at:      0    1446    1683 
   Size:       1446     237
 
NspI r_CATG'y

Cuts at:      0    1290    1683 
   Size:       1290     393
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1055    1683 
   Size:       1055     628
 
PmlI CAC'GTG

Cuts at:      0     107    1683 
   Size:        107    1576
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     823     855    1683 
   Size:        823      32     828

  Fragments arranged by size: 

                 828     823      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1176    1647    1683 
   Size:       1176     471      36

  Fragments arranged by size: 

                1176     471      36
 
PspOMI G'GGCC_C

Cuts at:      0      75     299    1495    1576    1683 
   Size:         75     224    1196      81     107

  Fragments arranged by size: 

                1196     224     107      81      75
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     592     624    1683 
   Size:        592      32    1059

  Fragments arranged by size: 

                1059     592      32
 
PstI C_TGCA'G

Cuts at:      0     986    1271    1683 
   Size:        986     285     412

  Fragments arranged by size: 

                 986     412     285
 
PvuII CAG'CTG

Cuts at:      0      30    1272    1683 
   Size:         30    1242     411

  Fragments arranged by size: 

                1242     411      30
 
SacII CC_GC'GG

Cuts at:      0    1235    1683 
   Size:       1235     448
 
SanDI GG'GwC_CC

Cuts at:      0    1176    1683 
   Size:       1176     507
 
SfcI C'TryA_G

Cuts at:      0     982    1267    1617    1683 
   Size:        982     285     350      66

  Fragments arranged by size: 

                 982     350     285      66
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1584    1683 
   Size:       1584      99
 
SmlI C'TyrA_G

Cuts at:      0     526    1500    1683 
   Size:        526     974     183

  Fragments arranged by size: 

                 974     526     183
 
SphI G_CATG'C

Cuts at:      0    1290    1683 
   Size:       1290     393
 
SspI AAT'ATT

Cuts at:      0     426    1683 
   Size:        426    1257
 
StuI AGG'CCT

Cuts at:      0     703    1683 
   Size:        703     980
 
StyI C'CwwG_G

Cuts at:      0      69     269    1491    1683 
   Size:         69     200    1222     192

  Fragments arranged by size: 

                1222     200     192      69
 
TatI w'GTAC_w

Cuts at:      0    1193    1457    1683 
   Size:       1193     264     226

  Fragments arranged by size: 

                1193     264     226
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     394     426     953     985    1683 
   Size:        394      32     527      32     698

  Fragments arranged by size: 

                 698     527     394      32      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     739    1683 
   Size:        739     944
 

 Enzymes that do cut:

     AccI     AcuI     AfeI    AflII     AhdI     AleI    AlwNI     ApaI
     ApoI     AseI     AvaI    AvrII    BanII     BbsI    BfrBI     BglI
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspMI    BsrBI   BssHII   BstAPI    BstBI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI   DraIII     EaeI     EciI  Eco57MI
    EcoNI EcoO109I     FspI    HaeII    Hin4I   HincII     MmeI     MslI
   MspA1I     NdeI     NsiI     NspI    PflMI     PmlI     PpiI    PpuMI
   PspOMI     PsrI     PstI    PvuII    SacII    SanDI     SfcI     SfiI
     SmlI     SphI     SspI     StuI     StyI     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI   AflIII     AgeI     AlfI     AloI
    ApaLI     AscI    AsiSI     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI    BglII    BmgBI     BmtI    BpuEI    BsaBI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrFI
    BsrGI    BssSI  BstZ17I     ClaI    CspCI     DraI     DrdI     EagI
     EarI  EcoICRI    EcoRI    EcoRV     FalI     FseI    FspAI  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI
     PfoI     PmeI    PshAI     PsiI    PspXI     PvuI    RsrII     SacI
     SalI     SapI     SbfI     ScaI    SexAI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SwaI    TaqII  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI
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