Restriction map for pF1KA0594
##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AgeI        
Alw44I      AlwNI       Aor51HI     ApaI        ApaLI       
AscI        Asp718I     AspI        AviII       AvrII       
BalI        BamHI       BbrPI       BbvCI       BcgI        
BclI        BglI        BlnI        BlpI        Bpu1102I    
BsaBI       BsaWI       BseAI       BsiEI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstEII      BstPI       BstUI       
BstZ17I     CelII       Cfr10I      ClaI        CpoI        
DraIII      DrdI        EagI        EclXI       Eco47III    
Eco52I      EcoO65I     EcoRV       EcoT22I     FbaI        
FseI        FspI        HindIII     KpnI        KspI        
MamI        MluI        MluNI       MroI        MscI        
MvnI        NaeI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PstI        PvuI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SgrAI       
SmaI        SnaBI       SphI        Sse8387I    SspBI       
StuI        SwaI        Tth111I     Van91I      XcmI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII          21  GACGTC               GACGT^C
AccI         3211  GTCTAC               GT^[AC][GT]AC
AhdI           67  GACCCTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI         1720  GATCC                GGATC(4/5)
AocI          538  CCTCAGG              CC^T[ACGT]AGG
AseI         2815  ATTAAT               AT^TAAT
AsnI         2815  ATTAAT               AT^TAAT
AspEI          67  GACCCTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI         3275  GGCGCC               G^G[CT][GA]CC
BbeI         3275  GGCGCC               GGCGC^C
BciVI        2169  GGATAC               GTATCC(6/5)
BcnI          134  CCGGG                CC^[GC]GG
BglII        1654  AGATCT               A^GATCT
BpmI         2047  CTGGAG               CTGGAG(16/14)
BsgI         2489  GTGCAG               GTGCAG(16/14)
BsmBI        1221  GAGACG               CGTCTC(1/5)
BspHI        1758  TCATGA               T^CATGA
BspLU11I      919  ACATGT               A^CATGT
BspMI         300  ACCTGC               ACCTGC(4/8)
BstXI        3238  CCAAACACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1654  AGATCT               [GA]^GATC[CT]
Bsu36I        538  CCTCAGG              CC^T[ACGT]AGG
DraII          80  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EaeI         2366  TGGCCG               [CT]^GGCC[GA]
Eam1105I       67  GACCCTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        538  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1569  CCTCAAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I       80  AGGTCCC              [GA]G^G[ACGT]CC[CT]
HaeII        3275  GGCGCC               [GA]GCGC^[CT]
HapII         134  CCGG                 C^CGG
HgaI          948  GCGTC                GACGC(5/10)
HpaI         2706  GTTAAC               GTT^AAC
HpaII         134  CCGG                 C^CGG
KasI         3275  GGCGCC               G^GCGCC
MflI         1654  AGATCT               [GA]^GATC[CT]
MspI          134  CCGG                 C^CGG
NarI         3275  GGCGCC               GG^CGCC
NciI          134  CCGGG                CC^[GC]GG
NspI          919  ACATGT               [GA]CATG^[CT]
PaeR7I        673  CTCGAG               C^TCGAG
PpuMI          80  AGGTCCC              [GA]G^G[AT]CC[CT]
PshBI        2815  ATTAAT               AT^TAAT
PvuII         112  CAGCTG               CAG^CTG
RcaI         1758  TCATGA               T^CATGA
SacI           53  GAGCTC               GAGCT^C
ScaI         2938  AGTACT               AGT^ACT
SfoI         3275  GGCGCC               GGC^GCC
SpeI          858  ACTAGT               A^CTAGT
SspI          505  AATATT               AAT^ATT
XhoI          673  CTCGAG               C^TCGAG
XhoII        1654  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AcyI           21  GACGTC               G[GA]^CG[CT]C
             3275  GGCGCC              
AflII         651  CTTAAG               C^TTAAG
             1524  CTTAAG              
AflIII        919  ACATGT               A^C[GA][CT]GT
             1385  ACACGT              
Asp700I       826  GAAAATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2987  GAAGTGTTTC          
AspHI          53  GAGCTC               G[AT]GC[AT]^C
             2050  GAGCAC              
BanII          53  GAGCTC               G[GA]GC[CT]^C
             1447  GAGCCC              
BbsI           18  GAAGAC               GAAGAC(2/6)
             1955  GAAGAC              
BfrI          651  CTTAAG               C^TTAAG
             1524  CTTAAG              
BpuAI          18  GAAGAC               GAAGAC(2/6)
             1955  GAAGAC              
BsaAI        1386  CACGTA               [CT]AC^GT[GA]
             2027  TACGTG              
BsaHI          21  GACGTC               G[GA]^CG[CT]C
             3275  GGCGCC              
BsaI           65  GAGACC               GGTCTC(1/5)
              676  GAGACC              
BsiHKAI        53  GAGCTC               G[AT]GC[AT]^C
             2050  GAGCAC              
BsrI         2118  ACTGG                ACTGG(1/-1)
              528  CCAGT               
BstBI        2912  TTCGAA               TT^CGAA
             2930  TTCGAA              
BstNI         211  CCTGG                CC^[AT]GG
             2484  CCTGG               
Cac8I        1068  GCAGGC               GC[ACGT]^[ACGT]GC
             1998  GCAAGC              
CfoI         1918  GCGC                 GCG^C
             3276  GCGC                
EcoRI          99  GAATTC               G^AATTC
             2909  GAATTC              
EcoRII        211  CCTGG                ^CC[AT]GG
             2484  CCTGG               
FauI          108  CCCGC                CCCGC(4/6)
             1127  CCCGC               
HhaI         1918  GCGC                 GCG^C
             3276  GCGC                
Hin1I          21  GACGTC               G[GA]^CG[CT]C
             3275  GGCGCC              
HinP1I       1918  GCGC                 G^CGC
             3276  GCGC                
HincII         24  GTCAAC               GT[CT]^[GA]AC
             2706  GTTAAC              
HindII         24  GTCAAC               GT[CT]^[GA]AC
             2706  GTTAAC              
MfeI          605  CAATTG               C^AATTG
             2663  CAATTG              
MspA1I        112  CAGCTG               C[AC]G^C[GT]G
              118  CCGCTG              
MunI          605  CAATTG               C^AATTG
             2663  CAATTG              
MvaI          211  CCTGG                CC^[AT]GG
             2484  CCTGG               
NcoI          811  CCATGG               C^CATGG
             2564  CCATGG              
NspV         2912  TTCGAA               TT^CGAA
             2930  TTCGAA              
SapI         1093  GAAGAGC              GCTCTTC(1/4)
             2191  GAAGAGC             
SfuI         2912  TTCGAA               TT^CGAA
             2930  TTCGAA              
XbaI          355  TCTAGA               T^CTAGA
             1290  TCTAGA              
XmnI          826  GAAAATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2987  GAAGTGTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AvaI        AvaII       BbvI        BfaI        BmyI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BssKI       BstF5I      
Cfr13I      DdeI        DpnII       DraI        DsaI        
EarI        EcoT14I     Fnu4HI      FokI        HaeIII      
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MslI        MwoI        NdeI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     
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