Restriction map for pF1KA0591
##################################
# NO CUT ENZYMES (   48 enzymes) #
##################################

Acc65I      AflII       AflIII      AgeI        ApaI        
AscI        Asp718I     AviII       BbrPI       BclI        
BfrI        BsiWI       BsmBI       BspLU11I    BssHII      
Bst1107I    BstEII      BstPI       BstZ17I     CpoI        
DrdI        EcoO65I     FbaI        FseI        FspI        
KpnI        MluI        NotI        NruI        NspI        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshAI       PvuI        RsrII       SalI        SbfI        
ScaI        SexAI       SgrAI       SpeI        SphI        
Sse8387I    SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AatII        1362  GACGTC               GACGT^C
AccII         999  CGCG                 CG^CG
AclI         1424  AACGTT               AA^CGTT
AhdI         3133  GACTTTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       4798  GTGCAC               G^TGCAC
AocI         3967  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1802  AGCGCT               AGC^GCT
ApaLI        4798  GTGCAC               G^TGCAC
AspEI        3133  GACTTTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        1566  CCTAGG               C^CTAGG
BamHI        4851  GGATCC               G^GATCC
BbeI         1694  GGCGCC               GGCGC^C
BcgI         2703  CGAGCGCCTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI         1566  CCTAGG               C^CTAGG
BsaAI        1816  TACGTA               [CT]AC^GT[GA]
BspDI        1192  ATCGAT               AT^CGAT
BspHI        2442  TCATGA               T^CATGA
BspMI        2148  GCAGGT               ACCTGC(4/8)
BssSI        1919  CACGAG               CACGAG(-5/-1)
BstBI        4302  TTCGAA               TT^CGAA
BstUI         999  CGCG                 CG^CG
Bsu36I       3967  CCTGAGG              CC^T[ACGT]AGG
ClaI         1192  ATCGAT               AT^CGAT
DraI         1428  TTTAAA               TTT^AAA
EagI         2744  CGGCCG               C^GGCCG
Eam1105I     3133  GACTTTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2744  CGGCCG               C^GGCCG
Eco47III     1802  AGCGCT               AGC^GCT
Eco52I       2744  CGGCCG               C^GGCCG
Eco81I       3967  CCTGAGG              CC^T[ACGT]AGG
EcoT22I        75  ATGCAT               ATGCA^T
HpaI         1603  GTTAAC               GTT^AAC
KasI         1694  GGCGCC               G^GCGCC
KspI          998  CCGCGG               CCGC^GG
MfeI         4841  CAATTG               C^AATTG
MunI         4841  CAATTG               C^AATTG
MvnI          999  CGCG                 CG^CG
NarI         1694  GGCGCC               GG^CGCC
NdeI         4409  CATATG               CA^TATG
NheI         1260  GCTAGC               G^CTAGC
NsiI           75  ATGCAT               ATGCA^T
NspV         4302  TTCGAA               TT^CGAA
PaeR7I         50  CTCGAG               C^TCGAG
Psp1406I     1424  AACGTT               AA^CGTT
RcaI         2442  TCATGA               T^CATGA
SacII         998  CCGCGG               CCGC^GG
SapI         1732  GAAGAGC              GCTCTTC(1/4)
SfiI         2238  GGCCCAATGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1694  GGCGCC               GGC^GCC
SfuI         4302  TTCGAA               TT^CGAA
SmaI         4964  CCCGGG               CCC^GGG
SnaBI        1816  TACGTA               TAC^GTA
XhoI           50  CTCGAG               C^TCGAG
XmaCI        4964  CCCGGG               C^CCGGG
XmaI         4964  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AccI         1395  GTCTAC               GT^[AC][GT]AC
             4915  GTAGAC              
AccIII       1748  TCCGGA               T^CCGGA
             2252  TCCGGA              
AseI          811  ATTAAT               AT^TAAT
             3247  ATTAAT              
AsnI          811  ATTAAT               AT^TAAT
             3247  ATTAAT              
AspI         3197  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4149  GACCTTGTC           
BanI          775  GGTGCC               G^G[CT][GA]CC
             1694  GGCGCC              
BciVI        5116  GGATAC               GTATCC(6/5)
             2952  GTATCC              
BsaBI        1177  GATATTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4300  GATTCGAATC          
BsaWI        1748  TCCGGA               [AT]^CCGG[AT]
             2252  TCCGGA              
BseAI        1748  TCCGGA               T^CCGGA
             2252  TCCGGA              
BsmI         2812  GCATTC               GAATGC(1/-1)
             3445  GCATTC              
Bsp1407I      218  TGTACA               T^GTACA
             3027  TGTACA              
BspEI        1748  TCCGGA               T^CCGGA
             2252  TCCGGA              
BsrBI        1690  GAGCGG               CCGCTC(-3/-3)
             1713  GAGCGG              
BsrFI        2827  GCCGGC               [GA]^CCGG[CT]
             4188  GCCGGC              
BsrGI         218  TGTACA               T^GTACA
             3027  TGTACA              
Cfr10I       2827  GCCGGC               [GA]^CCGG[CT]
             4188  GCCGGC              
DraIII        652  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2971  CACAGGGTG           
EcoRI        3074  GAATTC               G^AATTC
             4786  GAATTC              
HincII       1603  GTTAAC               GT[CT]^[GA]AC
             3814  GTTGAC              
HindII       1603  GTTAAC               GT[CT]^[GA]AC
             3814  GTTGAC              
HindIII      1480  AAGCTT               A^AGCTT
             5413  AAGCTT              
MamI         1177  GATATTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4300  GATTCGAATC          
MroI         1748  TCCGGA               T^CCGGA
             2252  TCCGGA              
NaeI         2827  GCCGGC               GCC^GGC
             4188  GCCGGC              
NcoI         1304  CCATGG               C^CATGG
             5279  CCATGG              
NgoMIV       2827  GCCGGC               G^CCGGC
             4188  GCCGGC              
PflFI        3197  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4149  GACCTTGTC           
PflMI         514  CCACTTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2970  CCACAGGGTGG         
PshBI         811  ATTAAT               AT^TAAT
             3247  ATTAAT              
PstI         2171  CTGCAG               CTGCA^G
             3983  CTGCAG              
SspBI         218  TGTACA               T^GTACA
             3027  TGTACA              
StuI         2135  AGGCCT               AGG^CCT
             2452  AGGCCT              
Tth111I      3197  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4149  GACCTTGTC           
Van91I        514  CCACTTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2970  CCACAGGGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  120 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaI        AvaII       BalI        BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BglI        
BglII       BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrDI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstXI       BstYI       Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoRV       EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII       XmnI        
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