Restriction map for pF1KA0580
##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       Acc65I      AccIII      AcyI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BlnI        
BsaAI       BsaBI       BsaHI       BseAI       BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrGI       BssHII      
BssSI       Bst1107I    BstZ17I     Bsu36I      CfoI        
ClaI        CpoI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoT22I     FauI        FseI        FspI        HaeII       
HhaI        Hin1I       HinP1I      HpaI        KasI        
KpnI        KspI        MamI        MluI        MroI        
NaeI        NarI        NgoMIV      NotI        NruI        
NsiI        PacI        PflFI       PmaCI       PmeI        
PmlI        PshAI       PvuI        RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SfoI        
SgrAI       SmaI        SnaBI       SphI        Sse8387I    
SspBI       Tth111I     XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccI         2814  GTCTAC               GT^[AC][GT]AC
AccII         451  CGCG                 CG^CG
AclI         4649  AACGTT               AA^CGTT
AflII        3177  CTTAAG               C^TTAAG
AflIII       2934  ACGTGT               A^C[GA][CT]GT
AgeI         4373  ACCGGT               A^CCGGT
ApaI         2896  GGGCCC               GGGCC^C
AvaI         1193  CTCGAG               C^[CT]CG[GA]G
BalI         4624  TGGCCA               TGG^CCA
BcgI         4191  GCAGGTGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI         4727  CCCGG                CC^[GC]GG
BfrI         3177  CTTAAG               C^TTAAG
BglI         4949  GCCATAAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         3366  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     3366  GCTCAGC              GC^T[ACGT]AGC
BsmFI        3828  GGGAC                GGGAC(10/14)
BsoBI        1193  CTCGAG               C^[CT]CG[GA]G
BsrDI        2243  GCAATG               GCAATG(2/0)
BsrFI        4373  ACCGGT               [GA]^CCGG[CT]
BstEII        163  GGTCACC              G^GT[ACGT]ACC
BstPI         163  GGTCACC              G^GT[ACGT]ACC
BstUI         451  CGCG                 CG^CG
CelII        3366  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       4373  ACCGGT               [GA]^CCGG[CT]
DraII        3860  AGGACCT              [GA]G^G[ACGT]CC[CT]
EaeI         4624  TGGCCA               [CT]^GGCC[GA]
EcoNI        4102  CCTACAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     3860  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       163  GGTCACC              G^GT[ACGT]ACC
EcoRI         978  GAATTC               G^AATTC
HgaI         4158  GCGTC                GACGC(5/10)
HindIII      3513  AAGCTT               A^AGCTT
MfeI         3317  CAATTG               C^AATTG
MluNI        4624  TGGCCA               TGG^CCA
MscI         4624  TGGCCA               TGG^CCA
MunI         3317  CAATTG               C^AATTG
MvnI          451  CGCG                 CG^CG
NciI         4727  CCCGG                CC^[GC]GG
NdeI         2332  CATATG               CA^TATG
NspI         1493  GCATGT               [GA]CATG^[CT]
PaeR7I       1193  CTCGAG               C^TCGAG
PinAI        4373  ACCGGT               A^CCGGT
PpuMI        3860  AGGACCT              [GA]G^G[AT]CC[CT]
Psp1406I     4649  AACGTT               AA^CGTT
SacI         3610  GAGCTC               GAGCT^C
SapI         4147  GAAGAGC              GCTCTTC(1/4)
SexAI        4235  ACCTGGT              A^CC[AT]GGT
SfiI         4948  GGCCATAAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI         3835  ACTAGT               A^CTAGT
StuI         4956  AGGCCT               AGG^CCT
SwaI         2885  ATTTAAAT             ATTT^AAAT
XbaI         2475  TCTAGA               T^CTAGA
XcmI         2116  CCAGATCCTGACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1193  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AspHI        3610  GAGCTC               G[AT]GC[AT]^C
             4849  GAGCAC              
BanI         2830  GGCACC               G^G[CT][GA]CC
             4544  GGCACC              
BanII        2896  GGGCCC               G[GA]GC[CT]^C
             3610  GAGCTC              
BglII        2182  AGATCT               A^GATCT
             2915  AGATCT              
BsaI         1006  GAGACC               GGTCTC(1/5)
             2528  GAGACC              
BsiHKAI      3610  GAGCTC               G[AT]GC[AT]^C
             4849  GAGCAC              
BspMI        3331  GCAGGT               ACCTGC(4/8)
             4191  GCAGGT              
BstAPI       1096  GCAGCTACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2574  GCAAAGTCTGC         
BstBI        1566  TTCGAA               TT^CGAA
             4868  TTCGAA              
BstXI          81  CCATGAGTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4501  CCAACAAGCTGG        
DraI         2886  TTTAAA               TTT^AAA
             3517  TTTAAA              
EcoRV        1775  GATATC               GAT^ATC
             4038  GATATC              
NcoI         3276  CCATGG               C^CATGG
             4829  CCATGG              
NheI         2230  GCTAGC               G^CTAGC
             2556  GCTAGC              
NspV         1566  TTCGAA               TT^CGAA
             4868  TTCGAA              
PvuII        3399  CAGCTG               CAG^CTG
             4631  CAGCTG              
SfuI         1566  TTCGAA               TT^CGAA
             4868  TTCGAA              


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AseI        AsnI        Asp700I     
AvaII       BbsI        BbvI        BciVI       BclI        
BfaI        BmyI        BpmI        BpuAI       BsaJI       
BsaWI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmI        Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DsaI        EarI        EcoRII      
EcoT14I     FbaI        Fnu4HI      FokI        HaeIII      
HapII       HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PflMI       
PleI        PshBI       PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XhoII       XmnI        
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