Restriction map for pF1KA0577
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# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AflII       
AgeI        AhdI        Alw44I      ApaLI       ApoI        
AscI        AseI        AsnI        AspEI       AviII       
AvrII       BalI        BbeI        BbrPI       BcgI        
BciVI       BfrI        BglII       BlnI        BsaAI       
BsaBI       BseAI       BsiEI       BsiWI       BsmBI       
BsmI        BspEI       BspLU11I    BssHII      BstAPI      
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco52I      EcoNI       EcoRI       EcoT22I     FspI        
HpaI        KasI        KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MunI        NarI        NheI        NotI        NruI        
NsiI        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        ScaI        SfiI        SfoI        SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
Tru9I       Tsp509I     Van91I      XbaI        XhoI        


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# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI         2211  GTATAC               GT^[AC][GT]AC
AccII         995  CGCG                 CG^CG
AflIII       2769  ACGTGT               A^C[GA][CT]GT
AocI          381  CCTCAGG              CC^T[ACGT]AGG
AspI         2575  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI        2150  CCTCAGC              CCTCAGC(-5/-2)
BclI         1604  TGATCA               T^GATCA
BsaWI         653  ACCGGA               [AT]^CCGG[AT]
Bsp1407I     2706  TGTACA               T^GTACA
BspDI        2718  ATCGAT               AT^CGAT
BspHI        2987  TCATGA               T^CATGA
BsrGI        2706  TGTACA               T^GTACA
Bst1107I     2211  GTATAC               GTA^TAC
BstBI        1709  TTCGAA               TT^CGAA
BstEII       2626  GGTGACC              G^GT[ACGT]ACC
BstPI        2626  GGTGACC              G^GT[ACGT]ACC
BstUI         995  CGCG                 CG^CG
BstZ17I      2211  GTATAC               GTA^TAC
Bsu36I        381  CCTCAGG              CC^T[ACGT]AGG
ClaI         2718  ATCGAT               AT^CGAT
DrdI         2575  GACAAGGTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          662  CCATGG               C^C[GA][CT]GG
Eco81I        381  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      2626  GGTGACC              G^GT[ACGT]ACC
EcoRV        1828  GATATC               GAT^ATC
FbaI         1604  TGATCA               T^GATCA
FseI          170  GGCCGGCC             GGCCGG^CC
MvnI          995  CGCG                 CG^CG
NcoI          662  CCATGG               C^CATGG
NdeI         2354  CATATG               CA^TATG
NspV         1709  TTCGAA               TT^CGAA
PflFI        2575  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI         2987  TCATGA               T^CATGA
SexAI        2633  ACCTGGT              A^CC[AT]GGT
SfuI         1709  TTCGAA               TT^CGAA
SgrAI           8  CGCCGGCG             C[GA]^CCGG[CT]G
SspBI        2706  TGTACA               T^GTACA
StuI         2147  AGGCCT               AGG^CCT
Tth111I      2575  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1052  CCAAGTATCAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

Acc65I         94  GGTACC               G^GTACC
              207  GGTACC              
AcyI            6  GACGCC               G[GA]^CG[CT]C
             1696  GACGCC              
Aor51HI        23  AGCGCT               AGC^GCT
              104  AGCGCT              
ApaI          263  GGGCCC               GGGCC^C
             1025  GGGCCC              
Asp700I       508  GAACGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2196  GAAGTGCTTC          
Asp718I        94  GGTACC               G^GTACC
              207  GGTACC              
BamHI        2070  GGATCC               G^GATCC
             2312  GGATCC              
BanI           94  GGTACC               G^G[CT][GA]CC
              207  GGTACC              
BsaHI           6  GACGCC               G[GA]^CG[CT]C
             1696  GACGCC              
BsaI         1081  GAGACC               GGTCTC(1/5)
             2782  GGTCTC              
BspMI        1724  GCAGGT               ACCTGC(4/8)
             2176  GCAGGT              
BsrDI        2025  CATTGC               GCAATG(2/0)
             2282  GCAATG              
BstXI        1373  CCATGAGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1815  CCAGCCCCCTGG        
DraIII         46  CACTCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2655  CACACAGTG           
EaeI          641  TGGCCG               [CT]^GGCC[GA]
             1382  TGGCCG              
Eco47III       23  AGCGCT               AGC^GCT
              104  AGCGCT              
Hin1I           6  GACGCC               G[GA]^CG[CT]C
             1696  GACGCC              
KpnI           94  GGTACC               GGTAC^C
              207  GGTACC              
NspI          896  ACATGC               [GA]CATG^[CT]
             1961  ACATGC              
PstI         2139  CTGCAG               CTGCA^G
             2189  CTGCAG              
SapI         2091  GAAGAGC              GCTCTTC(1/4)
             3112  GAAGAGC             
XmnI          508  GAACGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2196  GAAGTGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  106 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AvaI        AvaII       BanII       BbsI        
BbvI        BcnI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrBI       
BsrFI       BsrI        BssKI       BssSI       BstF5I      
BstNI       BstYI       Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HincII      HindII      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MslI        MspA1I      
MspI        MvaI        MwoI        NaeI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       PleI        
PpuMI       PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
SmlI        StyI        TaqI        TfiI        TseI        
Tsp45I      TspRI       TthHB8I     XhoII       XmaCI       
XmaI        
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