Restriction map for pF1KA0577
 (Linear) (Six-base) MAPSORT of: KIAA0577.seq  Check: 6704  from: 1  to: 3123



KIAA0577.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:21  ..

AccI GT'mk_AC

Cuts at:      0    2212    3123 
   Size:       2212     911
 
Acc65I G'GTAC_C

Cuts at:      0      94     207    3123 
   Size:         94     113    2916

  Fragments arranged by size: 

                2916     113      94
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     499    1036    1637    3123 
   Size:        499     537     601    1486

  Fragments arranged by size: 

                1486     601     537     499
 
AfeI AGC'GCT

Cuts at:      0      25     106    3123 
   Size:         25      81    3017

  Fragments arranged by size: 

                3017      81      25
 
AflIII A'CryG_T

Cuts at:      0    2769    3123 
   Size:       2769     354
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     123    2214    2405    3123 
   Size:        123    2091     191     718

  Fragments arranged by size: 

                2091     718     191     123
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2062    2094    3123 
   Size:       2062      32    1029

  Fragments arranged by size: 

                2062    1029      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      87     522    1765    2257    2530    2792    3000    3123 
   Size:         87     435    1243     492     273     262     208     123

  Fragments arranged by size: 

                1243     492     435     273     262     208     123      87
            
 
ApaI G_GGCC'C

Cuts at:      0     267    1029    3123 
   Size:        267     762    2094

  Fragments arranged by size: 

                2094     762     267
 
AvaI C'yCGr_G

Cuts at:      0     176     239     260     839     911     926    1028    1388 
   Size:        176      63      21     579      72      15     102     360

Cuts at:   1388    1397    2732    3123 
   Size:          9    1335     391

  Fragments arranged by size: 

                1335     579     391     360     176     102      72      63
                  21      15       9
 
BamHI G'GATC_C

Cuts at:      0    2070    2312    3123 
   Size:       2070     242     811

  Fragments arranged by size: 

                2070     811     242
 
BanI G'GyrC_C

Cuts at:      0      94     207    3123 
   Size:         94     113    2916

  Fragments arranged by size: 

                2916     113      94
 
BanII G_rGCy'C

Cuts at:      0     267     809     963    1029    1052    1223    1637    1909 
   Size:        267     542     154      66      23     171     414     272

Cuts at:   1909    1925    2403    2423    2733    2762    3123 
   Size:         16     478      20     310      29     361

  Fragments arranged by size: 

                 542     478     414     361     310     272     267     171
                 154      66      29      23      20      16
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     308     656    1288    2895    3123 
   Size:        308     348     632    1607     228

  Fragments arranged by size: 

                1607     632     348     308     228
 
BbvCI CC'TCA_GC

Cuts at:      0    2151    3123 
   Size:       2151     972
 
BclI T'GATC_A

Cuts at:      0    1604    3123 
   Size:       1604    1519
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1393    1904    2946    3123 
   Size:       1393     511    1042     177

  Fragments arranged by size: 

                1393    1042     511     177
 
BlpI GC'TnA_GC

Cuts at:      0      61     545     707     784    3123 
   Size:         61     484     162      77    2339

  Fragments arranged by size: 

                2339     484     162      77      61
 
Bme1580I G_kGCm'C

Cuts at:      0     267    1029    2001    3123 
   Size:        267     762     972    1122

  Fragments arranged by size: 

                1122     972     762     267
 
BmgBI CAC'GTC

Cuts at:      0      75     777    2429    3123 
   Size:         75     702    1652     694

  Fragments arranged by size: 

                1652     702     694      75
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      75    2679    3123 
   Size:         75    2604     444

  Fragments arranged by size: 

                2604     444      75
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      63    1237    1290    1533    3123 
   Size:         63    1174      53     243    1590

  Fragments arranged by size: 

                1590    1174     243      63      53
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     292     337     544     757     895    1090    1096 
   Size:         40     252      45     207     213     138     195       6

Cuts at:   1096    1742    1867    3022    3046    3123 
   Size:        646     125    1155      24      77

  Fragments arranged by size: 

                1155     646     252     213     207     195     138     125
                  77      45      40      24       6
 
Bpu10I CC'TnA_GC

Cuts at:      0     671    1275    1631    2151    2307    3123 
   Size:        671     604     356     520     156     816

  Fragments arranged by size: 

                 816     671     604     520     356     156
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     619     748    1621    2260    2284    3123 
   Size:        619     129     873     639      24     839

  Fragments arranged by size: 

                 873     839     639     619     129      24
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1075    2788    3123 
   Size:       1075    1713     335

  Fragments arranged by size: 

                1713    1075     335
 
BsaHI Gr'CG_yC

Cuts at:      0       7    1697    3123 
   Size:          7    1690    1426

  Fragments arranged by size: 

                1690    1426       7
 
BsaWI w'CCGG_w

Cuts at:      0     653    3123 
   Size:        653    2470
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1039    1069    1740    1770    2371    2401    3123 
   Size:       1039      30     671      30     601      30     722

  Fragments arranged by size: 

                1039     722     671     601      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     136     337     421     541     772     961     985    1003 
   Size:        136     201      84     120     231     189      24      18

Cuts at:   1003    1087    1090    1093    1138    1231    1327    1870    2527 
   Size:         84       3       3      45      93      96     543     657

Cuts at:   2527    2910    3123 
   Size:        383     213

  Fragments arranged by size: 

                 657     543     383     231     213     201     189     136
                 120      96      93      84      84      45      24      18
                   3       3
 
BseYI C'CCAG_C

Cuts at:      0      26     856     983    1157    1814    3123 
   Size:         26     830     127     174     657    1309

  Fragments arranged by size: 

                1309     830     657     174     127      26
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28      93     151    1332    1440    3123 
   Size:         28      65      58    1181     108    1683

  Fragments arranged by size: 

                1683    1181     108      65      58      28
 
BsiHKAI G_wGCw'C

Cuts at:      0     809    1223    1637    1925    2423    3123 
   Size:        809     414     414     288     498     700

  Fragments arranged by size: 

                 809     700     498     414     414     288
 
Bsp1286I G_dGCh'C

Cuts at:      0     267     809     963    1029    1052    1223    1637    1909 
   Size:        267     542     154      66      23     171     414     272

Cuts at:   1909    1925    2001    2403    2423    2733    2762    3123 
   Size:         16      76     402      20     310      29     361

  Fragments arranged by size: 

                 542     414     402     361     310     272     267     171
                 154      76      66      29      23      20      16
 
BspHI T'CATG_A

Cuts at:      0    2987    3123 
   Size:       2987     136
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1715    2167    3123 
   Size:       1715     452     956

  Fragments arranged by size: 

                1715     956     452
 
BsrBI CCG'CTC

Cuts at:      0      69     549     798    3123 
   Size:         69     480     249    2325

  Fragments arranged by size: 

                2325     480     249      69
 
BsrDI GCAATG_nn'

Cuts at:      0    2024    2289    3123 
   Size:       2024     265     834

  Fragments arranged by size: 

                2024     834     265
 
BsrFI r'CCGG_y

Cuts at:      0       9     171     788    2454    3123 
   Size:          9     162     617    1666     669

  Fragments arranged by size: 

                1666     669     617     162       9
 
BsrGI T'GTAC_A

Cuts at:      0    2706    3123 
   Size:       2706     417
 
BssSI C'ACGA_G

Cuts at:      0     793    2233    3107    3123 
   Size:        793    1440     874      16

  Fragments arranged by size: 

                1440     874     793      16
 
BstBI TT'CG_AA

Cuts at:      0    1710    3123 
   Size:       1710    1413
 
BstEII G'GTnAC_C

Cuts at:      0    2626    3123 
   Size:       2626     497
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1380    1822    3123 
   Size:       1380     442    1301

  Fragments arranged by size: 

                1380    1301     442
 
BstYI r'GATC_y

Cuts at:      0     159     831     936    1292    1802    2070    2261    2312 
   Size:        159     672     105     356     510     268     191      51

Cuts at:   2312    2516    3066    3123 
   Size:        204     550      57

  Fragments arranged by size: 

                 672     550     510     356     268     204     191     159
                 105      57      51
 
BstZ17I GTA'TAC

Cuts at:      0    2213    3123 
   Size:       2213     910
 
Bsu36I CC'TnA_GG

Cuts at:      0     382    3123 
   Size:        382    2741
 
BtgI C'CryG_G

Cuts at:      0     662    3123 
   Size:        662    2461
 
BtsI GCAGTG_nn'

Cuts at:      0     227     324    1667    2362    2828    3123 
   Size:        227      97    1343     695     466     295

  Fragments arranged by size: 

                1343     695     466     295     227      97
 
ClaI AT'CG_AT

Cuts at:      0    2719    3123 
   Size:       2719     404
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1671    1706    3123 
   Size:       1671      35    1417

  Fragments arranged by size: 

                1671    1417      35
 
DraIII CAC_nnn'GTG

Cuts at:      0      51    2660    3123 
   Size:         51    2609     463

  Fragments arranged by size: 

                2609     463      51
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2581    3123 
   Size:       2581     542
 
EaeI y'GGCC_r

Cuts at:      0     641    1382    3123 
   Size:        641     741    1741

  Fragments arranged by size: 

                1741     741     641
 
EarI CTCTTCn'nnn_

Cuts at:      0     305     398     407     611    2086    2685    3107    3123 
   Size:        305      93       9     204    1475     599     422      16

  Fragments arranged by size: 

                1475     599     422     305     204      93      16       9
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     757    1174    2194    3123 
   Size:        757     417    1020     929

  Fragments arranged by size: 

                1020     929     757     417
 
EcoICRI GAG'CTC

Cuts at:      0     807    1221    1635    1923    2421    3123 
   Size:        807     414     414     288     498     702

  Fragments arranged by size: 

                 807     702     498     414     414     288
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     292     337     499     544     757     895    1036 
   Size:         40     252      45     162      45     213     138     141

Cuts at:   1036    1090    1096    1637    1742    1867    3022    3046    3123 
   Size:         54       6     541     105     125    1155      24      77

  Fragments arranged by size: 

                1155     541     252     213     162     141     138     125
                 105      77      54      45      45      40      24       6
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     264     519     732    1025    1574    1641    3123 
   Size:        264     255     213     293     549      67    1482

  Fragments arranged by size: 

                1482     549     293     264     255     213      67
 
EcoRV GAT'ATC

Cuts at:      0    1830    3123 
   Size:       1830    1293
 
FseI GG_CCGG'CC

Cuts at:      0     175    3123 
   Size:        175    2948
 
HaeII r_GCGC'y

Cuts at:      0      27     108     138     633     984    3123 
   Size:         27      81      30     495     351    2139

  Fragments arranged by size: 

                2139     495     351      81      30      27
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     937     969    1024    1056    1114    1146    1444    1476 
   Size:        937      32      55      32      58      32     298      32

Cuts at:   1476    1619    1651    3123 
   Size:        143      32    1472

  Fragments arranged by size: 

                1472     937     298     143      58      55      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    2547    2607    2861    3123 
   Size:       2547      60     254     262

  Fragments arranged by size: 

                2547     262     254      60
 
HindIII A'AGCT_T

Cuts at:      0     724    1414    1778    3123 
   Size:        724     690     364    1345

  Fragments arranged by size: 

                1345     724     690     364
 
KpnI G_GTAC'C

Cuts at:      0      98     211    3123 
   Size:         98     113    2912

  Fragments arranged by size: 

                2912     113      98
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     458    1650    3123 
   Size:        458    1192    1473

  Fragments arranged by size: 

                1473    1192     458
 
MslI CAynn'nnrTG

Cuts at:      0    1378    2062    2113    3123 
   Size:       1378     684      51    1010

  Fragments arranged by size: 

                1378    1010     684      51
 
MspA1I CmG'CkG

Cuts at:      0     561     856     978    2385    2504    2751    3123 
   Size:        561     295     122    1407     119     247     372

  Fragments arranged by size: 

                1407     561     372     295     247     122     119
 
NaeI GCC'GGC

Cuts at:      0      11     173     790    3123 
   Size:         11     162     617    2333

  Fragments arranged by size: 

                2333     617     162      11
 
NcoI C'CATG_G

Cuts at:      0     662    3123 
   Size:        662    2461
 
NdeI CA'TA_TG

Cuts at:      0    2355    3123 
   Size:       2355     768
 
NgoMIV G'CCGG_C

Cuts at:      0       9     171     788    3123 
   Size:          9     162     617    2335

  Fragments arranged by size: 

                2335     617     162       9
 
NspI r_CATG'y

Cuts at:      0     900    1965    3123 
   Size:        900    1065    1158

  Fragments arranged by size: 

                1158    1065     900
 
PasI CC'CwG_GG

Cuts at:      0     964    1822    3123 
   Size:        964     858    1301

  Fragments arranged by size: 

                1301     964     858
 
PfoI T'CCnGG_A

Cuts at:      0     587    1505    1883    1916    2339    3123 
   Size:        587     918     378      33     423     784

  Fragments arranged by size: 

                 918     784     587     423     378      33
 
PpuMI rG'GwC_Cy

Cuts at:      0     519     732    1574    1641    3123 
   Size:        519     213     842      67    1482

  Fragments arranged by size: 

                1482     842     519     213      67
 
PsiI TTA'TAA

Cuts at:      0    3047    3123 
   Size:       3047      76
 
PspOMI G'GGCC_C

Cuts at:      0     263    1025    3123 
   Size:        263     762    2098

  Fragments arranged by size: 

                2098     762     263
 
PstI C_TGCA'G

Cuts at:      0    2143    2193    3123 
   Size:       2143      50     930

  Fragments arranged by size: 

                2143     930      50
 
PvuII CAG'CTG

Cuts at:      0     856    2385    2504    2751    3123 
   Size:        856    1529     119     247     372

  Fragments arranged by size: 

                1529     856     372     247     119
 
SacI G_AGCT'C

Cuts at:      0     809    1223    1637    1925    2423    3123 
   Size:        809     414     414     288     498     700

  Fragments arranged by size: 

                 809     700     498     414     414     288
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2086    3107    3123 
   Size:       2086    1021      16

  Fragments arranged by size: 

                2086    1021      16
 
SexAI A'CCwGG_T

Cuts at:      0    2633    3123 
   Size:       2633     490
 
SfcI C'TryA_G

Cuts at:      0    1434    2139    2189    3123 
   Size:       1434     705      50     934

  Fragments arranged by size: 

                1434     934     705      50
 
SgrAI Cr'CCGG_yG

Cuts at:      0       9    3123 
   Size:          9    3114
 
SmaI CCC'GGG

Cuts at:      0     178     262     841    1399    2734    3123 
   Size:        178      84     579     558    1335     389

  Fragments arranged by size: 

                1335     579     558     389     178      84
 
SmlI C'TyrA_G

Cuts at:      0     634     727    1636    2239    2299    3123 
   Size:        634      93     909     603      60     824

  Fragments arranged by size: 

                 909     824     634     603      93      60
 
SrfI GCCC'GGGC

Cuts at:      0     262    3123 
   Size:        262    2861
 
StuI AGG'CCT

Cuts at:      0    2149    3123 
   Size:       2149     974
 
StyI C'CwwG_G

Cuts at:      0     155     662     902    1751    2412    3053    3123 
   Size:        155     507     240     849     661     641      70

  Fragments arranged by size: 

                 849     661     641     507     240     155      70
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1155    1303    1366    1396    3123 
   Size:       1155     148      63      30    1727

  Fragments arranged by size: 

                1727    1155     148      63      30
 
TatI w'GTAC_w

Cuts at:      0    2498    2706    3123 
   Size:       2498     208     417

  Fragments arranged by size: 

                2498     417     208
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     378     410     472     489     504     521    1740    1772 
   Size:        378      32      62      17      15      17    1219      32

Cuts at:   1772    3123 
   Size:       1351

  Fragments arranged by size: 

                1351    1219     378      62      32      32      17      17
                  15
 
Tth111I GACn'n_nGTC

Cuts at:      0    2578    3123 
   Size:       2578     545
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1059    3123 
   Size:       1059    2064
 
XmaI C'CCGG_G

Cuts at:      0     176     260     839    1397    2732    3123 
   Size:        176      84     579     558    1335     391

  Fragments arranged by size: 

                1335     579     558     391     176      84
 
XmnI GAAnn'nnTTC

Cuts at:      0     512    2200    3123 
   Size:        512    1688     923

  Fragments arranged by size: 

                1688     923     512
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI   AflIII     AlfI     AloI    AlwNI
     ApaI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BclI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRV     FseI    HaeII    Hin4I   HincII
  HindIII     KpnI     MmeI     MslI   MspA1I     NaeI     NcoI     NdeI
   NgoMIV     NspI     PasI     PfoI    PpuMI     PsiI   PspOMI     PstI
    PvuII     SacI     SapI    SexAI     SfcI    SgrAI     SmaI     SmlI
     SrfI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI    AflII     AgeI     AhdI     AleI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BcgI
    BciVI    BfrBI    BglII     BmtI    BsaAI    BsaBI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI   BssHII   BstAPI    BtgZI     DraI     EagI
    EcoNI    EcoRI     FalI     FspI    FspAI     HpaI     KasI     MfeI
     MluI     MscI     NarI     NheI     NotI     NruI     NsiI     PacI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI     SfiI
     SfoI     SgfI    SnaBI     SpeI     SphI     SspI     SwaI     XbaI
     XhoI     ZraI
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