################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI AscI Asp700I Asp718I AspEI AspI AviII AvrII BbrPI BcgI BciVI BfrI BlnI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRV EcoT22I FseI FspI HindIII HpaI KpnI KspI MamI MluI MroI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccI 2417 GTAGAC GT^[AC][GT]AC AccII 3227 CGCG CG^CG Alw44I 389 GTGCAC G^TGCAC ApaLI 389 GTGCAC G^TGCAC AseI 1431 ATTAAT AT^TAAT AsnI 1431 ATTAAT AT^TAAT AvaI 2633 CCCGGG C^[CT]CG[GA]G BamHI 3627 GGATCC G^GATCC BglI 679 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 3745 GCTCAGC GC^T[ACGT]AGC Bpu1102I 3745 GCTCAGC GC^T[ACGT]AGC BsaI 1276 GAGACC GGTCTC(1/5) BsoBI 2633 CCCGGG C^[CT]CG[GA]G BsrDI 647 CATTGC GCAATG(2/0) BssSI 2800 CACGAG CACGAG(-5/-1) BstUI 3227 CGCG CG^CG CelII 3745 GCTCAGC GC^T[ACGT]AGC DraI 1492 TTTAAA TTT^AAA DraIII 3421 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 1663 CCTGCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 520 GAATTC G^AATTC MfeI 137 CAATTG C^AATTG MunI 137 CAATTG C^AATTG MvnI 3227 CGCG CG^CG NaeI 2336 GCCGGC GCC^GGC NgoMIV 2336 GCCGGC G^CCGGC NheI 4069 GCTAGC G^CTAGC PshAI 3001 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1431 ATTAAT AT^TAAT SacI 2012 GAGCTC GAGCT^C SmaI 2633 CCCGGG CCC^GGG SphI 3894 GCATGC GCATG^C SspI 2546 AATATT AAT^ATT XmaCI 2633 CCCGGG C^CCGGG XmaI 2633 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 214 GAATTT [GA]^AATT[CT] 520 GAATTC Aor51HI 2483 AGCGCT AGC^GCT 2621 AGCGCT ApoI 214 GAATTT [GA]^AATT[CT] 520 GAATTC AspHI 389 GTGCAC G[AT]GC[AT]^C 2012 GAGCTC BbeI 56 GGCGCC GGCGC^C 2339 GGCGCC BglII 267 AGATCT A^GATCT 2852 AGATCT BsiHKAI 389 GTGCAC G[AT]GC[AT]^C 2012 GAGCTC BspMI 1662 ACCTGC ACCTGC(4/8) 4244 ACCTGC BsrBI 2322 CCGCTC CCGCTC(-3/-3) 2859 CCGCTC BstAPI 3922 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4183 GCAGCAGCTGC Eco47III 2483 AGCGCT AGC^GCT 2621 AGCGCT HgaI 1657 GACGC GACGC(5/10) 2829 GCGTC HincII 121 GTTGAC GT[CT]^[GA]AC 3642 GTCAAC HindII 121 GTTGAC GT[CT]^[GA]AC 3642 GTCAAC KasI 56 GGCGCC G^GCGCC 2339 GGCGCC MaeII 1635 ACGT A^CGT 1830 ACGT MslI 15 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2122 CACAAAGGTG NarI 56 GGCGCC GG^CGCC 2339 GGCGCC NspI 3894 GCATGC [GA]CATG^[CT] 4140 ACATGC PflMI 354 CCATGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3175 CCATCTCTTGG SfoI 56 GGCGCC GGC^GCC 2339 GGCGCC Van91I 354 CCATGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3175 CCATCTCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI AvaII BalI BanI BanII BbsI BbvCI BbvI BclI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII