Restriction map for pF1KA0542
 (Linear) (Six-base) MAPSORT of: KIAA0542.seq  Check: 871  from: 1  to: 3633



KIAA0542.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3440    3498    3633 
   Size:       3440      58     135

  Fragments arranged by size: 

                3440     135      58
 
AccI GT'mk_AC

Cuts at:      0    1294    3633 
   Size:       1294    2339
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     598    3633 
   Size:        598    3035
 
AfeI AGC'GCT

Cuts at:      0    2185    3633 
   Size:       2185    1448
 
AflIII A'CryG_T

Cuts at:      0    1438    3633 
   Size:       1438    2195
 
AleI CACnn'nnGTG

Cuts at:      0     968    1263    3633 
   Size:        968     295    2370

  Fragments arranged by size: 

                2370     968     295
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1281    2362    3633 
   Size:       1281    1081    1271

  Fragments arranged by size: 

                1281    1271    1081
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2040    2072    3403    3435    3633 
   Size:       2040      32    1331      32     198

  Fragments arranged by size: 

                2040    1331     198      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     792    1155    1169    1394    1560    1758    1821    1956 
   Size:        792     363      14     225     166     198      63     135

Cuts at:   1956    2162    2223    2265    2625    2736    3484    3552    3615 
   Size:        206      61      42     360     111     748      68      63

Cuts at:   3615    3627    3633 
   Size:         12       6

  Fragments arranged by size: 

                 792     748     363     360     225     206     198     166
                 135     111      68      63      63      61      42      14
                  12       6
 
ApaI G_GGCC'C

Cuts at:      0     747    2439    3096    3633 
   Size:        747    1692     657     537

  Fragments arranged by size: 

                1692     747     657     537
 
ApoI r'AATT_y

Cuts at:      0    1738    3633 
   Size:       1738    1895
 
AscI GG'CGCG_CC

Cuts at:      0    1059    3633 
   Size:       1059    2574
 
AvaI C'yCGr_G

Cuts at:      0    1997    3633 
   Size:       1997    1636
 
AvrII C'CTAG_G

Cuts at:      0    3000    3229    3633 
   Size:       3000     229     404

  Fragments arranged by size: 

                3000     404     229
 
BamHI G'GATC_C

Cuts at:      0     170    3633 
   Size:        170    3463
 
BanI G'GyrC_C

Cuts at:      0    1403    1583    2572    2815    3589    3633 
   Size:       1403     180     989     243     774      44

  Fragments arranged by size: 

                1403     989     774     243     180      44
 
BanII G_rGCy'C

Cuts at:      0     747    1362    1703    2439    2862    2888    2903    3096 
   Size:        747     615     341     736     423      26      15     193

Cuts at:   3096    3566    3633 
   Size:        470      67

  Fragments arranged by size: 

                 747     736     615     470     423     341     193      67
                  26      15
 
BbeI G_GCGC'C

Cuts at:      0    1407    3593    3633 
   Size:       1407    2186      40

  Fragments arranged by size: 

                2186    1407      40
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     146     363     490    2296    3633 
   Size:        146     217     127    1806    1337

  Fragments arranged by size: 

                1806    1337     217     146     127
 
BbvCI CC'TCA_GC

Cuts at:      0    2729    2879    3633 
   Size:       2729     150     754

  Fragments arranged by size: 

                2729     754     150
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1257    1291    3426    3460    3633 
   Size:       1257      34    2135      34     173

  Fragments arranged by size: 

                2135    1257     173      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    3313    3633 
   Size:       3313     320
 
BlpI GC'TnA_GC

Cuts at:      0     929    3011    3572    3633 
   Size:        929    2082     561      61

  Fragments arranged by size: 

                2082     929     561      61
 
Bme1580I G_kGCm'C

Cuts at:      0     717     747    1641    1695    2439    3096    3633 
   Size:        717      30     894      54     744     657     537

  Fragments arranged by size: 

                 894     744     717     657     537      54      30
 
BmgBI CAC'GTC

Cuts at:      0     479    1603    3633 
   Size:        479    1124    2030

  Fragments arranged by size: 

                2030    1124     479
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1107    3633 
   Size:       1107    2526
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1234    3633 
   Size:       1234    2399
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     655     739     940    1503    1701    1791    2251    2290 
   Size:        655      84     201     563     198      90     460      39

Cuts at:   2290    2524    2548    2917    3478    3550    3633 
   Size:        234      24     369     561      72      83

  Fragments arranged by size: 

                 655     563     561     460     369     234     201     198
                  90      84      83      72      39      24
 
Bpu10I CC'TnA_GC

Cuts at:      0     748    2729    2879    3633 
   Size:        748    1981     150     754

  Fragments arranged by size: 

                1981     754     748     150
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1645    2842    3373    3385    3633 
   Size:       1645    1197     531      12     248

  Fragments arranged by size: 

                1645    1197     531     248      12
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1190    2818    3633 
   Size:       1190    1628     815

  Fragments arranged by size: 

                1628    1190     815
 
BsaAI yAC'GTr

Cuts at:      0     591     677    2483    3633 
   Size:        591      86    1806    1150

  Fragments arranged by size: 

                1806    1150     591      86
 
BsaHI Gr'CG_yC

Cuts at:      0    1404    2937    3590    3633 
   Size:       1404    1533     653      43

  Fragments arranged by size: 

                1533    1404     653      43
 
BsaWI w'CCGG_w

Cuts at:      0     173    3633 
   Size:        173    3460
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     991    1021    1603    1633    1674    1704    3633 
   Size:        991      30     582      30      41      30    1929

  Fragments arranged by size: 

                1929     991     582      41      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     403    2523    2551    2896    3382    3487    3574    3633 
   Size:        403    2120      28     345     486     105      87      59

  Fragments arranged by size: 

                2120     486     403     345     105      87      59      28
            
 
BseYI C'CCAG_C

Cuts at:      0    1751    2213    2633    2709    2752    2800    2806    3316 
   Size:       1751     462     420      76      43      48       6     510

Cuts at:   3316    3633 
   Size:        317

  Fragments arranged by size: 

                1751     510     462     420     317      76      48      43
                   6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     226     781    1118    1144    1822    1843    2116 
   Size:        128      98     555     337      26     678      21     273

Cuts at:   2116    2311    3179    3373    3532    3633 
   Size:        195     868     194     159     101

  Fragments arranged by size: 

                 868     678     555     337     273     195     194     159
                 128     101      98      26      21
 
BsiHKAI G_wGCw'C

Cuts at:      0     676     956    1136    2341    2862    2903    3566    3633 
   Size:        676     280     180    1205     521      41     663      67

  Fragments arranged by size: 

                1205     676     663     521     280     180      67      41
            
 
Bsp1286I G_dGCh'C

Cuts at:      0     676     717     747     956    1136    1362    1641    1695 
   Size:        676      41      30     209     180     226     279      54

Cuts at:   1695    1703    2341    2439    2862    2888    2903    3096    3566 
   Size:          8     638      98     423      26      15     193     470

Cuts at:   3566    3633 
   Size:         67

  Fragments arranged by size: 

                 676     638     470     423     279     226     209     193
                 180      98      67      54      41      30      26      15
                   8
 
BspEI T'CCGG_A

Cuts at:      0     173    3633 
   Size:        173    3460
 
BspHI T'CATG_A

Cuts at:      0     540    3633 
   Size:        540    3093
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     896    1844    2380    2631    3440    3498    3633 
   Size:        896     948     536     251     809      58     135

  Fragments arranged by size: 

                 948     896     809     536     251     135      58
 
BsrBI CCG'CTC

Cuts at:      0     993    1336    1807    1812    3633 
   Size:        993     343     471       5    1821

  Fragments arranged by size: 

                1821     993     471     343       5
 
BsrDI GCAATG_nn'

Cuts at:      0    2792    3633 
   Size:       2792     841
 
BsrFI r'CCGG_y

Cuts at:      0     242    2255    3633 
   Size:        242    2013    1378

  Fragments arranged by size: 

                2013    1378     242
 
BsrGI T'GTAC_A

Cuts at:      0     458    3633 
   Size:        458    3175
 
BssHII G'CGCG_C

Cuts at:      0    1059    3633 
   Size:       1059    2574
 
BssSI C'ACGA_G

Cuts at:      0     409    3633 
   Size:        409    3224
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     266     558    1854    1956    2223    2265    2301    2625 
   Size:        266     292    1296     102     267      42      36     324

Cuts at:   2625    3387    3510    3633 
   Size:        762     123     123

  Fragments arranged by size: 

                1296     762     324     292     267     266     123     123
                 102      42      36
 
BstBI TT'CG_AA

Cuts at:      0     300     374    3633 
   Size:        300      74    3259

  Fragments arranged by size: 

                3259     300      74
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1047    2067    2487    3586    3633 
   Size:       1047    1020     420    1099      47

  Fragments arranged by size: 

                1099    1047    1020     420      47
 
BstYI r'GATC_y

Cuts at:      0     170    3371    3542    3633 
   Size:        170    3201     171      91

  Fragments arranged by size: 

                3201     171     170      91
 
BstZ17I GTA'TAC

Cuts at:      0    1295    3633 
   Size:       1295    2338
 
Bsu36I CC'TnA_GG

Cuts at:      0    3485    3633 
   Size:       3485     148
 
BtgI C'CryG_G

Cuts at:      0    1650    1991    3633 
   Size:       1650     341    1642

  Fragments arranged by size: 

                1650    1642     341
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1413    2730    3633 
   Size:       1413    1317     903

  Fragments arranged by size: 

                1413    1317     903
 
BtsI GCAGTG_nn'

Cuts at:      0     836    1258    2246    2311    2536    2906    3633 
   Size:        836     422     988      65     225     370     727

  Fragments arranged by size: 

                 988     836     727     422     370     225      65
 
DraIII CAC_nnn'GTG

Cuts at:      0    1854    3633 
   Size:       1854    1779
 
EaeI y'GGCC_r

Cuts at:      0     272    1037    1532    3260    3633 
   Size:        272     765     495    1728     373

  Fragments arranged by size: 

                1728     765     495     373     272
 
EarI CTCTTCn'nnn_

Cuts at:      0     309     901    1124    1297    2311    3633 
   Size:        309     592     223     173    1014    1322

  Fragments arranged by size: 

                1322    1014     592     309     223     173
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2368    2665    3633 
   Size:       2368     297     968

  Fragments arranged by size: 

                2368     968     297
 
EcoICRI GAG'CTC

Cuts at:      0    2860    2901    3564    3633 
   Size:       2860      41     663      69

  Fragments arranged by size: 

                2860     663      69      41
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     598     655     739     940    1503    1701    1791    2251 
   Size:        598      57      84     201     563     198      90     460

Cuts at:   2251    2290    2524    2548    2917    3478    3550    3633 
   Size:         39     234      24     369     561      72      83

  Fragments arranged by size: 

                 598     563     561     460     369     234     201     198
                  90      84      83      72      57      39      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1915    2566    3049    3619    3633 
   Size:       1915     651     483     570      14

  Fragments arranged by size: 

                1915     651     570     483      14
 
EcoO109I rG'GnC_Cy

Cuts at:      0     743     744    2064    2436    2776    3054    3092    3093 
   Size:        743       1    1320     372     340     278      38       1

Cuts at:   3093    3481    3612    3624    3633 
   Size:        388     131      12       9

  Fragments arranged by size: 

                1320     743     388     372     340     278     131      38
                  12       9       1       1
 
EcoRI G'AATT_C

Cuts at:      0    1738    3633 
   Size:       1738    1895
 
FspI TGC'GCA

Cuts at:      0     259    2963    3448    3633 
   Size:        259    2704     485     185

  Fragments arranged by size: 

                2704     485     259     185
 
HaeII r_GCGC'y

Cuts at:      0    1407    1870    2187    2539    3107    3579    3593    3633 
   Size:       1407     463     317     352     568     472      14      40

  Fragments arranged by size: 

                1407     568     472     463     352     317      40      14
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     615     647    2283    2315    3633 
   Size:        615      32    1636      32    1318

  Fragments arranged by size: 

                1636    1318     615      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1667    3633 
   Size:       1667    1966
 
KasI G'GCGC_C

Cuts at:      0    1403    3589    3633 
   Size:       1403    2186      44

  Fragments arranged by size: 

                2186    1403      44
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     167    2933    3633 
   Size:        167    2766     700

  Fragments arranged by size: 

                2766     700     167
 
MscI TGG'CCA

Cuts at:      0     274    1039    1534    3262    3633 
   Size:        274     765     495    1728     371

  Fragments arranged by size: 

                1728     765     495     371     274
 
MslI CAynn'nnrTG

Cuts at:      0     968    1263    3633 
   Size:        968     295    2370

  Fragments arranged by size: 

                2370     968     295
 
MspA1I CmG'CkG

Cuts at:      0    1152    1320    1953    2229    2262    2397    2421    2625 
   Size:       1152     168     633     276      33     135      24     204

Cuts at:   2625    2681    3219    3549    3633 
   Size:         56     538     330      84

  Fragments arranged by size: 

                1152     633     538     330     276     204     168     135
                  84      56      33      24
 
NarI GG'CG_CC

Cuts at:      0    1404    3590    3633 
   Size:       1404    2186      43

  Fragments arranged by size: 

                2186    1404      43
 
NcoI C'CATG_G

Cuts at:      0    1991    3633 
   Size:       1991    1642
 
NdeI CA'TA_TG

Cuts at:      0    2053    3633 
   Size:       2053    1580
 
NspI r_CATG'y

Cuts at:      0    1089    1234    3633 
   Size:       1089     145    2399

  Fragments arranged by size: 

                2399    1089     145
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2349    2693    2895    3552    3633 
   Size:       2349     344     202     657      81

  Fragments arranged by size: 

                2349     657     344     202      81
 
PfoI T'CCnGG_A

Cuts at:      0    1913    2564    3633 
   Size:       1913     651    1069

  Fragments arranged by size: 

                1913    1069     651
 
PmlI CAC'GTG

Cuts at:      0     677    2483    3633 
   Size:        677    1806    1150

  Fragments arranged by size: 

                1806    1150     677
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     521     553    3633 
   Size:        521      32    3080

  Fragments arranged by size: 

                3080     521      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    2064    2776    3633 
   Size:       2064     712     857

  Fragments arranged by size: 

                2064     857     712
 
PspOMI G'GGCC_C

Cuts at:      0     743    2435    3092    3633 
   Size:        743    1692     657     541

  Fragments arranged by size: 

                1692     743     657     541
 
PstI C_TGCA'G

Cuts at:      0     831    1874    2200    2228    2306    2465    2531    2630 
   Size:        831    1043     326      28      78     159      66      99

Cuts at:   2630    3339    3633 
   Size:        709     294

  Fragments arranged by size: 

                1043     831     709     326     294     159      99      78
                  66      28
 
PvuII CAG'CTG

Cuts at:      0    1152    1320    1953    2229    2262    2397    2625    3549 
   Size:       1152     168     633     276      33     135     228     924

Cuts at:   3549    3633 
   Size:         84

  Fragments arranged by size: 

                1152     924     633     276     228     168     135      84
                  33
 
SacI G_AGCT'C

Cuts at:      0    2862    2903    3566    3633 
   Size:       2862      41     663      67

  Fragments arranged by size: 

                2862     663      67      41
 
SanDI GG'GwC_CC

Cuts at:      0    2776    3633 
   Size:       2776     857
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1124    3633 
   Size:       1124    2509
 
ScaI AGT'ACT

Cuts at:      0    2034    3633 
   Size:       2034    1599
 
SexAI A'CCwGG_T

Cuts at:      0    2173    3633 
   Size:       2173    1460
 
SfcI C'TryA_G

Cuts at:      0     444     626     827    1248    1288    1324    1870    2196 
   Size:        444     182     201     421      40      36     546     326

Cuts at:   2196    2224    2302    2461    2527    2626    3335    3633 
   Size:         28      78     159      66      99     709     298

  Fragments arranged by size: 

                 709     546     444     421     326     298     201     182
                 159      99      78      66      40      36      28
 
SfoI GGC'GCC

Cuts at:      0    1405    3591    3633 
   Size:       1405    2186      42

  Fragments arranged by size: 

                2186    1405      42
 
SmlI C'TyrA_G

Cuts at:      0    1660    2821    3352    3364    3633 
   Size:       1660    1161     531      12     269

  Fragments arranged by size: 

                1660    1161     531     269      12
 
SphI G_CATG'C

Cuts at:      0    1234    3633 
   Size:       1234    2399
 
StuI AGG'CCT

Cuts at:      0     568     874     985    2545    2611    2976    3633 
   Size:        568     306     111    1560      66     365     657

  Fragments arranged by size: 

                1560     657     568     365     306     111      66
 
StyI C'CwwG_G

Cuts at:      0    1695    1991    2060    3000    3229    3633 
   Size:       1695     296      69     940     229     404

  Fragments arranged by size: 

                1695     940     404     296     229      69
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1869    2356    2766    3633 
   Size:       1869     487     410     867

  Fragments arranged by size: 

                1869     867     487     410
 
TatI w'GTAC_w

Cuts at:      0     458     521    2032    2496    3633 
   Size:        458      63    1511     464    1137

  Fragments arranged by size: 

                1511    1137     464     458      63
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     408     440    1603    1635    1674    1706    3633 
   Size:        408      32    1163      32      39      32    1927

  Fragments arranged by size: 

                1927    1163     408      39      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     138    3633 
   Size:        138    3495
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1998    3633 
   Size:       1998    1635
 
XmnI GAAnn'nnTTC

Cuts at:      0     370    1669    3633 
   Size:        370    1299    1964

  Fragments arranged by size: 

                1964    1299     370
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AleI     AlfI     AloI
    AlwNI     ApaI     ApoI     AscI     AvaI    AvrII    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FspI    HaeII    Hin4I  HindIII     KasI
     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NdeI     NspI
    PflMI     PfoI     PmlI     PpiI    PpuMI   PspOMI     PstI    PvuII
     SacI    SanDI     SapI     ScaI    SexAI     SfcI     SfoI     SmlI
     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI
     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AhdI    ApaLI     AseI
    AsiSI     BaeI    BciVI     BclI    BfrBI    BglII     BmtI    BsaBI
    BsiEI    BsiWI     BsmI    BsmBI   BstEII     ClaI    CspCI     DraI
     DrdI     EagI    EcoRV     FalI     FseI    FspAI   HincII     HpaI
     KpnI     MfeI     MluI     NaeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PmeI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI     SbfI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI     XbaI
     XhoI     XmaI     ZraI
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