Restriction map for
pF1KA0531
(Linear) (Six-base) MAPSORT of: KIAA0531.seq Check: 8193 from: 1 to: 2871
KIAA0531.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 847 1354 2108 2668 2871
Size: 847 507 754 560 203
Fragments arranged by size:
847 754 560 507 203
AfeI AGC'GCT
Cuts at: 0 2686 2871
Size: 2686 185
AflIII A'CryG_T
Cuts at: 0 573 825 1411 2871
Size: 573 252 586 1460
Fragments arranged by size:
1460 586 573 252
AlwNI CAG_nnn'CTG
Cuts at: 0 994 1086 1470 2223 2391 2424 2871
Size: 994 92 384 753 168 33 447
Fragments arranged by size:
994 753 447 384 168 92 33
ApaI G_GGCC'C
Cuts at: 0 2751 2871
Size: 2751 120
ApaLI G'TGCA_C
Cuts at: 0 504 2871
Size: 504 2367
ApoI r'AATT_y
Cuts at: 0 82 94 1653 2498 2850 2871
Size: 82 12 1559 845 352 21
Fragments arranged by size:
1559 845 352 82 21 12
AvaI C'yCGr_G
Cuts at: 0 1526 1822 2432 2871
Size: 1526 296 610 439
Fragments arranged by size:
1526 610 439 296
BamHI G'GATC_C
Cuts at: 0 6 2871
Size: 6 2865
BanI G'GyrC_C
Cuts at: 0 727 1696 2871
Size: 727 969 1175
Fragments arranged by size:
1175 969 727
BanII G_rGCy'C
Cuts at: 0 529 2019 2751 2871
Size: 529 1490 732 120
Fragments arranged by size:
1490 732 529 120
BbsI GAAGACnn'nnnn_
Cuts at: 0 135 915 2351 2871
Size: 135 780 1436 520
Fragments arranged by size:
1436 780 520 135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1803 1837 2383 2417 2871
Size: 1803 34 546 34 454
Fragments arranged by size:
1803 546 454 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 456 2871
Size: 456 2415
BfrBI ATG'CAT
Cuts at: 0 193 2871
Size: 193 2678
BglI GCCn_nnn'nGGC
Cuts at: 0 2725 2746 2871
Size: 2725 21 125
Fragments arranged by size:
2725 125 21
BglII A'GATC_T
Cuts at: 0 410 1259 1668 2871
Size: 410 849 409 1203
Fragments arranged by size:
1203 849 410 409
BlpI GC'TnA_GC
Cuts at: 0 1597 1615 1990 2871
Size: 1597 18 375 881
Fragments arranged by size:
1597 881 375 18
Bme1580I G_kGCm'C
Cuts at: 0 508 2751 2871
Size: 508 2243 120
Fragments arranged by size:
2243 508 120
BmgBI CAC'GTC
Cuts at: 0 1414 2871
Size: 1414 1457
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1278 2365 2871
Size: 1278 1087 506
Fragments arranged by size:
1278 1087 506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 1108 1189 1248 1402 1447 1492 2128
Size: 400 708 81 59 154 45 45 636
Cuts at: 2128 2380 2542 2614 2656 2871
Size: 252 162 72 42 215
Fragments arranged by size:
708 636 400 252 215 162 154 81
72 59 45 45 42
BsaI GGTCTCn'nnnn_
Cuts at: 0 103 931 2871
Size: 103 828 1940
Fragments arranged by size:
1940 828 103
BsaAI yAC'GTr
Cuts at: 0 576 2871
Size: 576 2295
BsaBI GATnn'nnATC
Cuts at: 0 2213 2871
Size: 2213 658
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1109 1139 2356 2386 2871
Size: 1109 30 1217 30 485
Fragments arranged by size:
1217 1109 485 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1249 1270 1417 1489 1756 1759 1974 2191
Size: 1249 21 147 72 267 3 215 217
Cuts at: 2191 2377 2849 2871
Size: 186 472 22
Fragments arranged by size:
1249 472 267 217 215 186 147 72
22 21 3
BseYI C'CCAG_C
Cuts at: 0 308 700 1901 2871
Size: 308 392 1201 970
Fragments arranged by size:
1201 970 392 308
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2377 2871
Size: 2377 494
BsiEI CG_ry'CG
Cuts at: 0 2813 2871
Size: 2813 58
BsiHKAI G_wGCw'C
Cuts at: 0 508 2871
Size: 508 2363
BsmI GAATG_Cn'
Cuts at: 0 22 1863 2871
Size: 22 1841 1008
Fragments arranged by size:
1841 1008 22
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1420 2159 2871
Size: 1420 739 712
Fragments arranged by size:
1420 739 712
Bsp1286I G_dGCh'C
Cuts at: 0 508 529 2019 2751 2871
Size: 508 21 1490 732 120
Fragments arranged by size:
1490 732 508 120 21
BspHI T'CATG_A
Cuts at: 0 468 2871
Size: 468 2403
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 171 2579 2871
Size: 171 2408 292
Fragments arranged by size:
2408 292 171
BsrBI CCG'CTC
Cuts at: 0 513 1869 2871
Size: 513 1356 1002
Fragments arranged by size:
1356 1002 513
BsrFI r'CCGG_y
Cuts at: 0 38 2871
Size: 38 2833
BsrGI T'GTAC_A
Cuts at: 0 1766 2871
Size: 1766 1105
BssHII G'CGCG_C
Cuts at: 0 2642 2871
Size: 2642 229
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2391 2871
Size: 2391 480
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2736 2871
Size: 2736 135
BstYI r'GATC_y
Cuts at: 0 6 65 410 1259 1646 1668 2421 2871
Size: 6 59 345 849 387 22 753 450
Fragments arranged by size:
849 753 450 387 345 59 22 6
Bsu36I CC'TnA_GG
Cuts at: 0 530 1130 2871
Size: 530 600 1741
Fragments arranged by size:
1741 600 530
BtgI C'CryG_G
Cuts at: 0 72 113 284 365 1754 2612 2871
Size: 72 41 171 81 1389 858 259
Fragments arranged by size:
1389 858 259 171 81 72 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 119 2871
Size: 119 2752
BtsI GCAGTG_nn'
Cuts at: 0 2389 2484 2871
Size: 2389 95 387
Fragments arranged by size:
2389 387 95
DraI TTT'AAA
Cuts at: 0 99 2871
Size: 99 2772
EaeI y'GGCC_r
Cuts at: 0 2738 2810 2871
Size: 2738 72 61
Fragments arranged by size:
2738 72 61
EagI C'GGCC_G
Cuts at: 0 2810 2871
Size: 2810 61
EarI CTCTTCn'nnn_
Cuts at: 0 1374 1951 1967 1991 2850 2871
Size: 1374 577 16 24 859 21
Fragments arranged by size:
1374 859 577 24 21 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 2632 2767 2871
Size: 19 2613 135 104
Fragments arranged by size:
2613 135 104 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 847 1108 1189 1248 1354 1402 1447
Size: 400 447 261 81 59 106 48 45
Cuts at: 1447 1492 2108 2128 2380 2542 2614 2656 2668
Size: 45 616 20 252 162 72 42 12
Cuts at: 2668 2871
Size: 203
Fragments arranged by size:
616 447 400 261 252 203 162 106
81 72 59 48 45 45 42 20
12
EcoO109I rG'GnC_Cy
Cuts at: 0 1467 2747 2871
Size: 1467 1280 124
Fragments arranged by size:
1467 1280 124
EcoRV GAT'ATC
Cuts at: 0 345 2871
Size: 345 2526
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 238 474 2871
Size: 238 236 2397
Fragments arranged by size:
2397 238 236
HaeII r_GCGC'y
Cuts at: 0 1832 2107 2688 2871
Size: 1832 275 581 183
Fragments arranged by size:
1832 581 275 183
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 928 960 1251 1283 1335 1367 1486 1518
Size: 928 32 291 32 52 32 119 32
Cuts at: 1518 1780 1812 1908 1940 2356 2388 2871
Size: 262 32 96 32 416 32 483
Fragments arranged by size:
928 483 416 291 262 119 96 52
32 32 32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 2242 2871
Size: 2242 629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1328 1868 2435 2830 2871
Size: 1328 540 567 395 41
Fragments arranged by size:
1328 567 540 395 41
MscI TGG'CCA
Cuts at: 0 2740 2871
Size: 2740 131
MslI CAynn'nnrTG
Cuts at: 0 28 952 2871
Size: 28 924 1919
Fragments arranged by size:
1919 924 28
MspA1I CmG'CkG
Cuts at: 0 74 1320 1542 1890 2151 2550 2871
Size: 74 1246 222 348 261 399 321
Fragments arranged by size:
1246 399 348 321 261 222 74
NcoI C'CATG_G
Cuts at: 0 284 365 1754 2871
Size: 284 81 1389 1117
Fragments arranged by size:
1389 1117 284 81
NdeI CA'TA_TG
Cuts at: 0 138 2871
Size: 138 2733
NsiI A_TGCA'T
Cuts at: 0 195 2871
Size: 195 2676
NspI r_CATG'y
Cuts at: 0 197 829 1941 2871
Size: 197 632 1112 930
Fragments arranged by size:
1112 930 632 197
PciI A'CATG_T
Cuts at: 0 825 2871
Size: 825 2046
PflMI CCAn_nnn'nTGG
Cuts at: 0 285 315 1086 1470 2871
Size: 285 30 771 384 1401
Fragments arranged by size:
1401 771 384 285 30
PfoI T'CCnGG_A
Cuts at: 0 2045 2417 2555 2871
Size: 2045 372 138 316
Fragments arranged by size:
2045 372 316 138
PmlI CAC'GTG
Cuts at: 0 576 2871
Size: 576 2295
PsiI TTA'TAA
Cuts at: 0 233 2258 2871
Size: 233 2025 613
Fragments arranged by size:
2025 613 233
PspOMI G'GGCC_C
Cuts at: 0 2747 2871
Size: 2747 124
PstI C_TGCA'G
Cuts at: 0 889 2871
Size: 889 1982
PvuII CAG'CTG
Cuts at: 0 1320 1542 1890 2151 2550 2871
Size: 1320 222 348 261 399 321
Fragments arranged by size:
1320 399 348 321 261 222
SacII CC_GC'GG
Cuts at: 0 75 2871
Size: 75 2796
SapI GCTCTTCn'nnn_
Cuts at: 0 1991 2850 2871
Size: 1991 859 21
Fragments arranged by size:
1991 859 21
SfcI C'TryA_G
Cuts at: 0 885 1229 1272 2871
Size: 885 344 43 1599
Fragments arranged by size:
1599 885 344 43
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2725 2746 2871
Size: 2725 21 125
Fragments arranged by size:
2725 125 21
SmaI CCC'GGG
Cuts at: 0 1528 2871
Size: 1528 1343
SmlI C'TyrA_G
Cuts at: 0 1822 2871
Size: 1822 1049
SspI AAT'ATT
Cuts at: 0 636 1203 2871
Size: 636 567 1668
Fragments arranged by size:
1668 636 567
StyI C'CwwG_G
Cuts at: 0 284 365 1151 1439 1754 2657 2871
Size: 284 81 786 288 315 903 214
Fragments arranged by size:
903 786 315 288 284 214 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 865 2871
Size: 865 2006
TatI w'GTAC_w
Cuts at: 0 1766 2871
Size: 1766 1105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1109 1141 2871
Size: 1109 32 1730
Fragments arranged by size:
1730 1109 32
Tth111I GACn'n_nGTC
Cuts at: 0 942 2370 2871
Size: 942 1428 501
Fragments arranged by size:
1428 942 501
XbaI T'CTAG_A
Cuts at: 0 607 2374 2871
Size: 607 1767 497
Fragments arranged by size:
1767 607 497
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 456 2871
Size: 456 2415
XhoI C'TCGA_G
Cuts at: 0 1822 2871
Size: 1822 1049
XmaI C'CCGG_G
Cuts at: 0 1526 2871
Size: 1526 1345
Enzymes that do cut:
AcuI AfeI AflIII AlwNI ApaI ApaLI ApoI AvaI
BamHI BanI BanII BbsI BcgI BciVI BfrBI BglI
BglII BlpI Bme1580I BmgBI BplI BpmI BsaI BsaAI
BsaBI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssHII
BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRV FalI
HaeII Hin4I HindIII MmeI MscI MslI MspA1I NcoI
NdeI NsiI NspI PciI PflMI PfoI PmlI PsiI
PspOMI PstI PvuII SacII SapI SfcI SfiI SmaI
SmlI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlfI AloI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BclI BmrI BmtI Bpu10I BpuEI BsaHI
BsaWI BsiWI BspEI BsrDI BssSI BstBI BstEII BstZ17I
ClaI CspCI DraIII DrdI EcoICRI EcoNI EcoRI FseI
FspI FspAI HincII HpaI KasI KpnI MfeI MluI
NaeI NarI NgoMIV NheI NotI NruI PacI PasI
PmeI PpiI PpuMI PshAI PspXI PsrI PvuI RsrII
SacI SalI SanDI SbfI ScaI SexAI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI StuI SwaI XmnI
ZraI