(Circular) (Six-base) MAPSORT of: pF1KA0399.seq Check: 9011 from: 1 to: 11996 pF1KA0399 11996 bp With 182 enzymes: SgfI * June 20, 2008 10:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1044 6249 6665 1044 Size: 5205 416 6375 Fragments arranged by size: 6375 5205 416 AatII G_ACGT'C Cuts at: 4787 4787 Size: 11996 AccI GT'mk_AC Cuts at: 6563 9034 6563 Size: 2471 9525 Acc65I G'GTAC_C Cuts at: 2210 9012 2210 Size: 6802 5194 AclI AA'CG_TT Cuts at: 11836 11836 Size: 11996 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 535 784 1075 1687 1913 1975 2302 3064 3322 Size: 249 291 612 226 62 327 762 258 Cuts at: 3322 3852 4559 4667 4798 4903 6299 6838 7219 Size: 530 707 108 131 105 1396 539 381 Cuts at: 7219 7270 8364 9821 10253 11000 11268 535 Size: 51 1094 1457 432 747 268 1263 Fragments arranged by size: 1457 1396 1263 1094 762 747 707 612 539 530 432 381 327 291 268 258 249 226 131 108 105 62 51 AfeI AGC'GCT Cuts at: 236 2179 236 Size: 1943 10053 AflIII A'CryG_T Cuts at: 512 737 4628 5408 9348 10452 11429 512 Size: 225 3891 780 3940 1104 977 1079 Fragments arranged by size: 3940 3891 1104 1079 977 780 225 AgeI A'CCGG_T Cuts at: 6039 10384 6039 Size: 4345 7651 AleI CACnn'nnGTG Cuts at: 131 2610 2612 4823 131 Size: 2479 2 2211 7304 Fragments arranged by size: 7304 2479 2211 2 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1637 4380 6365 7859 8942 1637 Size: 2743 1985 1494 1083 4691 Fragments arranged by size: 4691 2743 1985 1494 1083 AlwNI CAG_nnn'CTG Cuts at: 956 1024 1066 1665 2784 3111 4003 6277 7806 Size: 68 42 599 1119 327 892 2274 1529 Cuts at: 7806 8916 9301 10868 956 Size: 1110 385 1567 2084 Fragments arranged by size: 2274 2084 1567 1529 1119 1110 892 599 385 327 68 42 ApaI G_GGCC'C Cuts at: 175 2964 6418 7059 175 Size: 2789 3454 641 5112 Fragments arranged by size: 5112 3454 2789 641 ApaLI G'TGCA_C Cuts at: 741 1108 1587 2255 2808 10766 741 Size: 367 479 668 553 7958 1971 Fragments arranged by size: 7958 1971 668 553 479 367 ApoI r'AATT_y Cuts at: 531 1223 1936 2424 3478 3523 3667 4393 5036 Size: 692 713 488 1054 45 144 726 643 Cuts at: 5036 5313 6227 7319 8293 8585 9000 11349 11605 Size: 277 914 1092 974 292 415 2349 256 Cuts at: 11605 531 Size: 922 Fragments arranged by size: 2349 1092 1054 974 922 914 726 713 692 643 488 415 292 277 256 144 45 AseI AT'TA_AT Cuts at: 20 20 Size: 11996 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 11996 AvaI C'yCGr_G Cuts at: 198 216 2451 2930 4694 6015 7052 7135 9016 Size: 18 2235 479 1764 1321 1037 83 1881 Cuts at: 9016 198 Size: 3178 Fragments arranged by size: 3178 2235 1881 1764 1321 1037 479 83 18 AvrII C'CTAG_G Cuts at: 5538 5538 Size: 11996 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5481 5514 5481 Size: 33 11963 BamHI G'GATC_C Cuts at: 2829 6207 9021 2829 Size: 3378 2814 5804 Fragments arranged by size: 5804 3378 2814 BanI G'GyrC_C Cuts at: 430 1004 1891 2210 4582 4746 8272 9012 9426 Size: 574 887 319 2372 164 3526 740 414 Cuts at: 9426 9674 9709 430 Size: 248 35 2717 Fragments arranged by size: 3526 2717 2372 887 740 574 414 319 248 164 35 BanII G_rGCy'C Cuts at: 175 1582 2000 2964 2980 4655 6022 6418 7059 Size: 1407 418 964 16 1675 1367 396 641 Cuts at: 7059 7493 7631 8036 8813 9010 10040 175 Size: 434 138 405 777 197 1030 2131 Fragments arranged by size: 2131 1675 1407 1367 1030 964 777 641 434 418 405 396 197 138 16 BbeI G_GCGC'C Cuts at: 434 9430 9678 434 Size: 8996 248 2752 Fragments arranged by size: 8996 2752 248 BbsI GAAGACnn'nnnn_ Cuts at: 143 746 3858 4372 6514 143 Size: 603 3112 514 2142 5625 Fragments arranged by size: 5625 3112 2142 603 514 BbvCI CC'TCA_GC Cuts at: 482 482 Size: 11996 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 667 701 5913 5947 8815 8849 667 Size: 34 5212 34 2868 34 3814 Fragments arranged by size: 5212 3814 2868 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3447 7640 7713 9894 10655 11996 3447 Size: 4193 73 2181 761 1341 3447 Fragments arranged by size: 4193 3447 2181 1341 761 73 BclI T'GATC_A Cuts at: 4107 5267 5621 6575 6926 9516 4107 Size: 1160 354 954 351 2590 6587 Fragments arranged by size: 6587 2590 1160 954 354 351 BglI GCCn_nnn'nGGC Cuts at: 270 5845 6698 8624 8721 270 Size: 5575 853 1926 97 3545 Fragments arranged by size: 5575 3545 1926 853 97 BglII A'GATC_T Cuts at: 813 2414 3069 5217 9511 11548 813 Size: 1601 655 2148 4294 2037 1261 Fragments arranged by size: 4294 2148 2037 1601 1261 655 BlpI GC'TnA_GC Cuts at: 562 6101 7063 7370 7661 9108 562 Size: 5539 962 307 291 1447 3450 Fragments arranged by size: 5539 3450 1447 962 307 291 Bme1580I G_kGCm'C Cuts at: 175 205 745 1112 1591 1896 2259 2651 2812 Size: 30 540 367 479 305 363 392 161 Cuts at: 2812 2964 4585 4751 6053 6418 6753 7059 8802 Size: 152 1621 166 1302 365 335 306 1743 Cuts at: 8802 8838 9621 9714 10770 175 Size: 36 783 93 1056 1401 Fragments arranged by size: 1743 1621 1401 1302 1056 783 540 479 392 367 365 363 335 306 305 166 161 152 93 36 30 BmgBI CAC'GTC Cuts at: 1879 4629 6447 8763 1879 Size: 2750 1818 2316 5112 Fragments arranged by size: 5112 2750 2316 1818 BmrI ACTGGGnnnn_n' Cuts at: 195 465 1890 3759 3987 6717 7670 9208 9289 Size: 270 1425 1869 228 2730 953 1538 81 Cuts at: 9289 9624 11768 195 Size: 335 2144 423 Fragments arranged by size: 2730 2144 1869 1538 1425 953 423 335 270 228 81 BmtI G_CTAG'C Cuts at: 6254 6254 Size: 11996 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2994 4720 10363 2994 Size: 1726 5643 4627 Fragments arranged by size: 5643 4627 1726 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 215 280 418 439 678 742 830 1675 2482 Size: 65 138 21 239 64 88 845 807 Cuts at: 2482 3080 4136 4450 6460 7618 7797 8050 8110 Size: 598 1056 314 2010 1158 179 253 60 Cuts at: 8110 215 Size: 4101 Fragments arranged by size: 4101 2010 1158 1056 845 807 598 314 253 239 179 138 88 65 64 60 21 Bpu10I CC'TnA_GC Cuts at: 482 1000 1999 3841 6338 482 Size: 518 999 1842 2497 6140 Fragments arranged by size: 6140 2497 1842 999 518 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 511 829 3291 3763 7495 9172 10051 10543 10841 Size: 318 2462 472 3732 1677 879 492 298 Cuts at: 10841 511 Size: 1666 Fragments arranged by size: 3732 2462 1677 1666 879 492 472 318 298 BsaI GGTCTCn'nnnn_ Cuts at: 201 1237 1278 1313 3346 4556 7373 8393 11700 Size: 1036 41 35 2033 1210 2817 1020 3307 Cuts at: 11700 201 Size: 497 Fragments arranged by size: 3307 2817 2033 1210 1036 1020 497 41 35 BsaAI yAC'GTr Cuts at: 2813 3389 7971 8007 9349 2813 Size: 576 4582 36 1342 5460 Fragments arranged by size: 5460 4582 1342 576 36 BsaBI GATnn'nnATC Cuts at: 6 2881 9510 11547 6 Size: 2875 6629 2037 455 Fragments arranged by size: 6629 2875 2037 455 BsaHI Gr'CG_yC Cuts at: 221 431 1648 3753 4784 6566 9427 9675 10377 Size: 210 1217 2105 1031 1782 2861 248 702 Cuts at: 10377 11873 221 Size: 1496 344 Fragments arranged by size: 2861 2105 1782 1496 1217 1031 702 344 248 210 BsaWI w'CCGG_w Cuts at: 1828 3926 6039 6055 9185 9706 10384 10658 10805 Size: 2098 2113 16 3130 521 678 274 147 Cuts at: 10805 11216 1828 Size: 411 2608 Fragments arranged by size: 3130 2608 2113 2098 678 521 411 274 147 16 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 2630 2660 5204 5234 2630 Size: 30 2544 30 9392 Fragments arranged by size: 9392 2544 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 472 1660 2293 2296 2560 3721 4747 5212 6082 Size: 1188 633 3 264 1161 1026 465 870 Cuts at: 6082 7043 7294 7332 8200 8332 472 Size: 961 251 38 868 132 4136 Fragments arranged by size: 4136 1188 1161 1026 961 870 868 633 465 264 251 132 38 3 BseYI C'CCAG_C Cuts at: 167 369 1117 4586 5171 5991 7058 7076 8148 Size: 202 748 3469 585 820 1067 18 1072 Cuts at: 8148 9421 10179 10756 11252 167 Size: 1273 758 577 496 911 Fragments arranged by size: 3469 1273 1072 1067 911 820 758 748 585 577 496 202 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 661 1357 2139 2371 3225 4922 5526 5873 7290 Size: 696 782 232 854 1697 604 347 1417 Cuts at: 7290 7525 7742 7864 661 Size: 235 217 122 4793 Fragments arranged by size: 4793 1697 1417 854 782 696 604 347 235 232 217 122 BsiEI CG_ry'CG Cuts at: 104 8495 9538 9584 10792 104 Size: 8391 1043 46 1208 1308 Fragments arranged by size: 8391 1308 1208 1043 46 BsiHKAI G_wGCw'C Cuts at: 728 745 1112 1582 1591 2000 2259 2774 2812 Size: 17 367 470 9 409 259 515 38 Cuts at: 2812 3052 3269 6446 6718 7376 7631 8036 8126 Size: 240 217 3177 272 658 255 405 90 Cuts at: 8126 8813 9010 9788 9978 10770 728 Size: 687 197 778 190 792 1954 Fragments arranged by size: 3177 1954 792 778 687 658 515 470 409 405 367 272 259 255 240 217 197 190 90 38 17 9 BsmI GAATG_Cn' Cuts at: 1083 2467 1083 Size: 1384 10612 BsmBI CGTCTCn'nnnn_ Cuts at: 2472 2922 3741 5561 2472 Size: 450 819 1820 8907 Fragments arranged by size: 8907 1820 819 450 Bsp1286I G_dGCh'C Cuts at: 175 205 728 745 1112 1582 1591 1896 2000 Size: 30 523 17 367 470 9 305 104 Cuts at: 2000 2259 2651 2774 2812 2964 2980 3052 3269 Size: 259 392 123 38 152 16 72 217 Cuts at: 3269 4585 4655 4751 6022 6053 6418 6446 6718 Size: 1316 70 96 1271 31 365 28 272 Cuts at: 6718 6753 7059 7376 7493 7631 8036 8126 8802 Size: 35 306 317 117 138 405 90 676 Cuts at: 8802 8813 8838 9010 9621 9714 9788 9978 10040 Size: 11 25 172 611 93 74 190 62 Cuts at: 10040 10770 175 Size: 730 1401 Fragments arranged by size: 1401 1316 1271 730 676 611 523 470 405 392 367 365 317 306 305 272 259 217 190 172 152 138 123 117 104 96 93 90 74 72 70 62 38 35 31 30 28 25 17 16 11 9 BspEI T'CCGG_A Cuts at: 11216 11216 Size: 11996 BspHI T'CATG_A Cuts at: 1574 5225 6138 7518 11172 1574 Size: 3651 913 1380 3654 2398 Fragments arranged by size: 3654 3651 2398 1380 913 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1044 1673 3044 4978 6249 6665 8873 9046 9260 Size: 629 1371 1934 1271 416 2208 173 214 Cuts at: 9260 9562 9943 1044 Size: 302 381 3097 Fragments arranged by size: 3097 2208 1934 1371 1271 629 416 381 302 214 173 BsrBI CCG'CTC Cuts at: 50 366 10288 10342 11826 11991 50 Size: 316 9922 54 1484 165 55 Fragments arranged by size: 9922 1484 316 165 55 54 BsrDI GCAATG_nn' Cuts at: 1013 1128 5414 5652 6126 6770 8924 9908 1013 Size: 115 4286 238 474 644 2154 984 3101 Fragments arranged by size: 4286 3101 2154 984 644 474 238 115 BsrFI r'CCGG_y Cuts at: 154 264 433 3607 4679 5765 6039 9994 10175 Size: 110 169 3174 1072 1086 274 3955 181 Cuts at: 10175 10384 154 Size: 209 1766 Fragments arranged by size: 3955 3174 1766 1086 1072 274 209 181 169 110 BsrGI T'GTAC_A Cuts at: 2600 2600 Size: 11996 BssSI C'ACGA_G Cuts at: 6844 10267 10625 6844 Size: 3423 358 8215 Fragments arranged by size: 8215 3423 358 BstAPI GCAn_nnn'nTGC Cuts at: 1024 2784 4552 5531 5651 8121 9373 1024 Size: 1760 1768 979 120 2470 1252 3647 Fragments arranged by size: 3647 2470 1768 1760 1252 979 120 BstBI TT'CG_AA Cuts at: 4251 10357 4251 Size: 6106 5890 BstEII G'GTnAC_C Cuts at: 5745 5745 Size: 11996 BstXI CCAn_nnnn'nTGG Cuts at: 1020 4573 6641 7716 8163 8845 8940 1020 Size: 3553 2068 1075 447 682 95 4076 Fragments arranged by size: 4076 3553 2068 1075 682 447 95 BstYI r'GATC_y Cuts at: 1 813 1426 2414 2829 3069 5217 5382 5517 Size: 812 613 988 415 240 2148 165 135 Cuts at: 5517 6207 6728 7217 8108 9021 9488 9511 9846 Size: 690 521 489 891 913 467 23 335 Cuts at: 9846 10092 11104 11190 11202 11548 1 Size: 246 1012 86 12 346 449 Fragments arranged by size: 2148 1012 988 913 891 812 690 613 521 489 467 449 415 346 335 246 240 165 135 86 23 12 Bsu36I CC'TnA_GG Cuts at: 2308 3818 4846 5555 2308 Size: 1510 1028 709 8749 Fragments arranged by size: 8749 1510 1028 709 BtgI C'CryG_G Cuts at: 107 191 248 375 1643 1765 2651 3077 4403 Size: 84 57 127 1268 122 886 426 1326 Cuts at: 4403 4727 5562 6419 6719 8156 8879 107 Size: 324 835 857 300 1437 723 3224 Fragments arranged by size: 3224 1437 1326 1268 886 857 835 723 426 324 300 127 122 84 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 319 1220 5669 8685 9946 10127 10290 319 Size: 901 4449 3016 1261 181 163 2025 Fragments arranged by size: 4449 3016 2025 1261 901 181 163 BtsI GCAGTG_nn' Cuts at: 138 726 957 1025 2259 3258 3677 4614 4923 Size: 588 231 68 1234 999 419 937 309 Cuts at: 4923 5509 5607 5904 6578 6789 7775 8358 9239 Size: 586 98 297 674 211 986 583 881 Cuts at: 9239 138 Size: 2895 Fragments arranged by size: 2895 1234 999 986 937 881 674 588 586 583 419 309 297 231 211 98 68 ClaI AT'CG_AT Cuts at: 5487 5487 Size: 11996 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 4481 4516 4481 Size: 35 11961 DraI TTT'AAA Cuts at: 977 8995 977 Size: 8018 3978 DraIII CAC_nnn'GTG Cuts at: 3264 7765 8468 3264 Size: 4501 703 6792 Fragments arranged by size: 6792 4501 703 DrdI GACnn_nn'nnGTC Cuts at: 2914 9702 10560 2914 Size: 6788 858 4350 Fragments arranged by size: 6788 4350 858 EaeI y'GGCC_r Cuts at: 266 2870 5706 5846 6528 7426 8492 8625 8679 Size: 2604 2836 140 682 898 1066 133 54 Cuts at: 8679 8942 9581 9755 10146 10173 11518 266 Size: 263 639 174 391 27 1345 744 Fragments arranged by size: 2836 2604 1345 1066 898 744 682 639 391 263 174 140 133 54 27 EagI C'GGCC_G Cuts at: 8492 9581 8492 Size: 1089 10907 EarI CTCTTCn'nnn_ Cuts at: 356 377 1538 1706 2746 2992 3040 4208 4369 Size: 21 1161 168 1040 246 48 1168 161 Cuts at: 4369 4502 4643 6361 6982 7409 7603 7933 8818 Size: 133 141 1718 621 427 194 330 885 Cuts at: 8818 10019 10229 356 Size: 1201 210 2123 Fragments arranged by size: 2123 1718 1201 1168 1161 1040 885 621 427 330 246 210 194 168 161 141 133 48 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 10204 10514 10660 11806 10204 Size: 310 146 1146 10394 Fragments arranged by size: 10394 1146 310 146 EcoICRI GAG'CTC Cuts at: 1580 1998 7629 8034 8811 9008 1580 Size: 418 5631 405 777 197 4568 Fragments arranged by size: 5631 4568 777 418 405 197 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 215 280 418 439 535 678 742 784 830 Size: 65 138 21 96 143 64 42 46 Cuts at: 830 1075 1675 1687 1913 1975 2302 2482 3064 Size: 245 600 12 226 62 327 180 582 Cuts at: 3064 3080 3322 3852 4136 4450 4559 4667 4798 Size: 16 242 530 284 314 109 108 131 Cuts at: 4798 4903 6299 6460 6838 7219 7270 7618 7797 Size: 105 1396 161 378 381 51 348 179 Cuts at: 7797 8050 8110 8364 9821 10253 11000 11268 215 Size: 253 60 254 1457 432 747 268 943 Fragments arranged by size: 1457 1396 943 747 600 582 530 432 381 378 348 327 314 284 268 254 253 245 242 226 180 179 161 143 138 131 109 108 105 96 65 64 62 60 51 46 42 21 16 12 EcoNI CCTnn'n_nnAGG Cuts at: 2047 4556 7882 8755 2047 Size: 2509 3326 873 5288 Fragments arranged by size: 5288 3326 2509 873 EcoO109I rG'GnC_Cy Cuts at: 171 522 639 2312 2960 2961 4071 5111 6033 Size: 351 117 1673 648 1 1110 1040 922 Cuts at: 6033 6415 6867 7158 7311 7479 9135 171 Size: 382 452 291 153 168 1656 3032 Fragments arranged by size: 3032 1673 1656 1110 1040 922 648 452 382 351 291 168 153 117 1 EcoRI G'AATT_C Cuts at: 531 1223 3478 5313 9000 531 Size: 692 2255 1835 3687 3527 Fragments arranged by size: 3687 3527 2255 1835 692 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5311 10015 5311 Size: 4704 7292 FspI TGC'GCA Cuts at: 9777 9777 Size: 11996 HaeII r_GCGC'y Cuts at: 238 256 434 1393 2181 3778 5840 7709 7857 Size: 18 178 959 788 1597 2062 1869 148 Cuts at: 7857 7871 9430 9678 10700 11679 238 Size: 14 1559 248 1022 979 555 Fragments arranged by size: 2062 1869 1597 1559 1022 979 959 788 555 248 178 148 18 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 637 669 1612 1644 2626 2658 4642 4674 5940 Size: 32 943 32 982 32 1984 32 1266 Cuts at: 5940 5972 5998 6030 8613 8645 9838 9868 9870 Size: 32 26 32 2583 32 1193 30 2 Cuts at: 9870 9900 637 Size: 30 2733 Fragments arranged by size: 2733 2583 1984 1266 1193 982 943 32 32 32 32 32 32 32 30 30 26 2 HincII GTy'rAC Cuts at: 1697 3214 4185 5586 9035 1697 Size: 1517 971 1401 3449 4658 Fragments arranged by size: 4658 3449 1517 1401 971 HindIII A'AGCT_T Cuts at: 1987 1987 Size: 11996 KasI G'GCGC_C Cuts at: 430 9426 9674 430 Size: 8996 248 2752 Fragments arranged by size: 8996 2752 248 KpnI G_GTAC'C Cuts at: 2214 9016 2214 Size: 6802 5194 MluI A'CGCG_T Cuts at: 11429 11429 Size: 11996 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 497 911 3523 3872 4418 4937 8474 10667 10851 Size: 414 2612 349 546 519 3537 2193 184 Cuts at: 10851 497 Size: 1642 Fragments arranged by size: 3537 2612 2193 1642 546 519 414 349 184 MscI TGG'CCA Cuts at: 2872 5848 8627 8944 9757 2872 Size: 2976 2779 317 813 5111 Fragments arranged by size: 5111 2976 2779 813 317 MslI CAynn'nnrTG Cuts at: 131 1018 2610 2612 2749 3413 4823 5230 5261 Size: 887 1592 2 137 664 1410 407 31 Cuts at: 5261 6494 9985 10112 131 Size: 1233 3491 127 2015 Fragments arranged by size: 3491 2015 1592 1410 1233 887 664 407 137 127 31 2 MspA1I CmG'CkG Cuts at: 151 193 250 377 783 3042 4645 5495 6804 Size: 42 57 127 406 2259 1603 850 1309 Cuts at: 6804 6816 6887 7014 7062 7665 7951 8082 8384 Size: 12 71 127 48 603 286 131 302 Cuts at: 8384 8900 9107 9421 9781 10794 11039 151 Size: 516 207 314 360 1013 245 1108 Fragments arranged by size: 2259 1603 1309 1108 1013 850 603 516 406 360 314 302 286 245 207 131 127 127 71 57 48 42 12 NaeI GCC'GGC Cuts at: 266 435 4681 10177 266 Size: 169 4246 5496 2085 Fragments arranged by size: 5496 4246 2085 169 NarI GG'CG_CC Cuts at: 431 9427 9675 431 Size: 8996 248 2752 Fragments arranged by size: 8996 2752 248 NcoI C'CATG_G Cuts at: 107 1643 2651 4727 6719 8156 107 Size: 1536 1008 2076 1992 1437 3947 Fragments arranged by size: 3947 2076 1992 1536 1437 1008 NdeI CA'TA_TG Cuts at: 8051 8051 Size: 11996 NgoMIV G'CCGG_C Cuts at: 264 433 4679 10175 264 Size: 169 4246 5496 2085 Fragments arranged by size: 5496 4246 2085 169 NheI G'CTAG_C Cuts at: 6250 6250 Size: 11996 NspI r_CATG'y Cuts at: 516 741 1047 1404 1712 4061 5349 5412 5928 Size: 225 306 357 308 2349 1288 63 516 Cuts at: 5928 8148 8174 9049 9549 10456 516 Size: 2220 26 875 500 907 2056 Fragments arranged by size: 2349 2220 2056 1288 907 875 516 500 357 308 306 225 63 26 PasI CC'CwG_GG Cuts at: 2965 3454 6516 2965 Size: 489 3062 8445 Fragments arranged by size: 8445 3062 489 PciI A'CATG_T Cuts at: 512 737 5408 10452 512 Size: 225 4671 5044 2056 Fragments arranged by size: 5044 4671 2056 225 PflMI CCAn_nnn'nTGG Cuts at: 2346 3111 3771 5580 5742 8181 2346 Size: 765 660 1809 162 2439 6161 Fragments arranged by size: 6161 2439 1809 765 660 162 PfoI T'CCnGG_A Cuts at: 407 4871 7307 7595 7916 8027 8418 407 Size: 4464 2436 288 321 111 391 3985 Fragments arranged by size: 4464 3985 2436 391 321 288 111 PmeI GTTT'AAAC Cuts at: 8995 8995 Size: 11996 PmlI CAC'GTG Cuts at: 7971 8007 7971 Size: 36 11960 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 11135 11167 11135 Size: 32 11964 PpuMI rG'GwC_Cy Cuts at: 2312 5111 6867 7158 7311 2312 Size: 2799 1756 291 153 6997 Fragments arranged by size: 6997 2799 1756 291 153 PshAI GACnn'nnGTC Cuts at: 3644 4568 3644 Size: 924 11072 PspOMI G'GGCC_C Cuts at: 171 2960 6414 7055 171 Size: 2789 3454 641 5112 Fragments arranged by size: 5112 3454 2789 641 PspXI vC'TCGA_Gb Cuts at: 6015 6015 Size: 11996 PstI C_TGCA'G Cuts at: 291 2266 2492 2522 2789 3041 4626 5504 6246 Size: 1975 226 30 267 252 1585 878 742 Cuts at: 6246 6615 7571 7835 8117 8858 8921 9043 9728 Size: 369 956 264 282 741 63 122 685 Cuts at: 9728 291 Size: 2559 Fragments arranged by size: 2559 1975 1585 956 878 742 741 685 369 282 267 264 252 226 122 63 30 PvuI CG_AT'CG Cuts at: 104 104 Size: 11996 PvuII CAG'CTG Cuts at: 151 783 3042 4645 6804 6816 7014 7062 7951 Size: 632 2259 1603 2159 12 198 48 889 Cuts at: 7951 8082 9421 9781 151 Size: 131 1339 360 2366 Fragments arranged by size: 2366 2259 2159 1603 1339 889 632 360 198 131 48 12 RsrII CG'GwC_CG Cuts at: 10191 10191 Size: 11996 SacI G_AGCT'C Cuts at: 1582 2000 7631 8036 8813 9010 1582 Size: 418 5631 405 777 197 4568 Fragments arranged by size: 5631 4568 777 418 405 197 SacII CC_GC'GG Cuts at: 194 251 378 194 Size: 57 127 11812 Fragments arranged by size: 11812 127 57 SalI G'TCGA_C Cuts at: 9033 9033 Size: 11996 SapI GCTCTTCn'nnn_ Cuts at: 356 2992 3040 4502 4643 8818 10019 10229 356 Size: 2636 48 1462 141 4175 1201 210 2123 Fragments arranged by size: 4175 2636 2123 1462 1201 210 141 48 SbfI CC_TGCA'GG Cuts at: 9043 9043 Size: 11996 ScaI AGT'ACT Cuts at: 7072 11513 7072 Size: 4441 7555 SexAI A'CCwGG_T Cuts at: 2345 2345 Size: 11996 SfcI C'TryA_G Cuts at: 33 287 583 1067 1430 2262 2488 2518 2785 Size: 254 296 484 363 832 226 30 267 Cuts at: 2785 3037 3312 4622 5500 5832 6155 6242 6260 Size: 252 275 1310 878 332 323 87 18 Cuts at: 6260 6611 7567 7831 8113 8475 8854 8917 9039 Size: 351 956 264 282 362 379 63 122 Cuts at: 9039 9724 10717 10908 33 Size: 685 993 191 1121 Fragments arranged by size: 1310 1121 993 956 878 832 685 484 379 363 362 351 332 323 296 282 275 267 264 254 252 226 191 122 87 63 30 18 SfoI GGC'GCC Cuts at: 432 9428 9676 432 Size: 8996 248 2752 Fragments arranged by size: 8996 2752 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 11996 SmaI CCC'GGG Cuts at: 218 9018 218 Size: 8800 3196 SmlI C'TyrA_G Cuts at: 526 844 2930 3270 3778 6015 7474 9151 10066 Size: 318 2086 340 508 2237 1459 1677 915 Cuts at: 10066 10558 10820 526 Size: 492 262 1702 Fragments arranged by size: 2237 2086 1702 1677 1459 915 508 492 340 318 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 11996 SphI G_CATG'C Cuts at: 5928 8174 9049 9549 5928 Size: 2246 875 500 8375 Fragments arranged by size: 8375 2246 875 500 SrfI GCCC'GGGC Cuts at: 218 218 Size: 11996 SspI AAT'ATT Cuts at: 2134 2134 Size: 11996 StuI AGG'CCT Cuts at: 463 589 5356 5972 463 Size: 126 4767 616 6487 Fragments arranged by size: 6487 4767 616 126 StyI C'CwwG_G Cuts at: 107 1643 2043 2591 2651 3009 4235 4727 4911 Size: 1536 400 548 60 358 1226 492 184 Cuts at: 4911 5538 6719 8156 9130 9484 107 Size: 627 1181 1437 974 354 2619 Fragments arranged by size: 2619 1536 1437 1226 1181 974 627 548 492 400 358 354 184 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 3935 4600 4686 5450 5753 5983 6667 7255 7421 Size: 665 86 764 303 230 684 588 166 Cuts at: 7421 7597 8384 8593 9579 9712 10171 10380 3935 Size: 176 787 209 986 133 459 209 5551 Fragments arranged by size: 5551 986 787 764 684 665 588 459 303 230 209 209 176 166 133 86 TatI w'GTAC_w Cuts at: 2529 2600 3152 7070 7337 11511 2529 Size: 71 552 3918 267 4174 3014 Fragments arranged by size: 4174 3918 3014 552 267 71 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2630 2662 2764 2796 6697 6729 8186 8218 11513 Size: 32 102 32 3901 32 1457 32 3295 Cuts at: 11513 11545 2630 Size: 32 3081 Fragments arranged by size: 3901 3295 3081 1457 102 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 9793 9793 Size: 11996 XbaI T'CTAG_A Cuts at: 9027 9027 Size: 11996 XcmI CCAnnnn_n'nnnnTGG Cuts at: 182 2136 9491 182 Size: 1954 7355 2687 Fragments arranged by size: 7355 2687 1954 XhoI C'TCGA_G Cuts at: 2930 6015 2930 Size: 3085 8911 XmaI C'CCGG_G Cuts at: 216 9016 216 Size: 8800 3196 ZraI GAC'GTC Cuts at: 4785 4785 Size: 11996 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI Enzymes that do not cut: AflII AhdI AloI AscI BfrBI BsiWI BssHII BstZ17I EcoRV FseI FspAI HpaI MfeI NotI NruI NsiI PacI PsiI PsrI SanDI SfiI SgrAI SnaBI SwaI XmnI