Restriction map for pF1KA0389
##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AgeI        AhdI        Aor51HI     ApaI        AscI        
AseI        AsnI        AspEI       AspI        AviII       
AvrII       BbrPI       BcgI        BciVI       BlnI        
BlpI        Bpu1102I    BsaBI       BsaI        BseAI       
BsiEI       BsiWI       BspDI       BspEI       BsrBI       
BssHII      Bst1107I    BstUI       BstZ17I     CelII       
ClaI        CpoI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoRI       EcoRV       EcoT22I     FseI        FspI        
KspI        MamI        MluI        MroI        MvnI        
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SapI        SbfI        
SexAI       SfiI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    StuI        Tsp45I      
Tth111I     XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I       1224  GGTACC               G^GTACC
AflII         901  CTTAAG               C^TTAAG
AflIII       2147  ACATGT               A^C[GA][CT]GT
Alw44I        960  GTGCAC               G^TGCAC
AocI         3039  CCTGAGG              CC^T[ACGT]AGG
ApaLI         960  GTGCAC               G^TGCAC
Asp718I      1224  GGTACC               G^GTACC
AvaI         2593  CCCGAG               C^[CT]CG[GA]G
BamHI        2187  GGATCC               G^GATCC
BbeI           24  GGCGCC               GGCGC^C
BfrI          901  CTTAAG               C^TTAAG
BglI         3088  GCCGACCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        3728  AGATCT               A^GATCT
BsaAI        3254  TACGTG               [CT]AC^GT[GA]
BsaWI        3135  ACCGGA               [AT]^CCGG[AT]
BsmBI        1715  GAGACG               CGTCTC(1/5)
BsoBI        2593  CCCGAG               C^[CT]CG[GA]G
Bsp1407I     2150  TGTACA               T^GTACA
BspLU11I     2147  ACATGT               A^CATGT
BspMI         907  GCAGGT               ACCTGC(4/8)
BsrDI        1427  GCAATG               GCAATG(2/0)
BsrFI         463  GCCGGC               [GA]^CCGG[CT]
BsrGI        2150  TGTACA               T^GTACA
BstAPI       1246  GCAAACAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2110  GGTTACC              G^GT[ACGT]ACC
BstPI        2110  GGTTACC              G^GT[ACGT]ACC
Bsu36I       3039  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        463  GCCGGC               [GA]^CCGG[CT]
DraII        3177  AGGTCCT              [GA]G^G[ACGT]CC[CT]
Eco81I       3039  CCTGAGG              CC^T[ACGT]AGG
EcoO109I     3177  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      2110  GGTTACC              G^GT[ACGT]ACC
HincII       2256  GTTAAC               GT[CT]^[GA]AC
HindII       2256  GTTAAC               GT[CT]^[GA]AC
HpaI         2256  GTTAAC               GTT^AAC
KasI           24  GGCGCC               G^GCGCC
KpnI         1224  GGTACC               GGTAC^C
NaeI          463  GCCGGC               GCC^GGC
NarI           24  GGCGCC               GG^CGCC
NgoMIV        463  GCCGGC               G^CCGGC
PflMI        1773  CCAGTTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        3177  AGGTCCT              [GA]G^G[AT]CC[CT]
SfoI           24  GGCGCC               GGC^GCC
SspBI        2150  TGTACA               T^GTACA
SwaI          200  ATTTAAAT             ATTT^AAAT
Van91I       1773  CCAGTTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         3641  CCATCCTACTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AcyI           24  GGCGCC               G[GA]^CG[CT]C
             1027  GGCGTC              
BalI         2645  TGGCCA               TGG^CCA
             3146  TGGCCA              
BbsI          585  GAAGAC               GAAGAC(2/6)
             2824  GAAGAC              
BbvCI        3439  CCTCAGC              CCTCAGC(-5/-2)
             2775  GCTGAGG             
BpuAI         585  GAAGAC               GAAGAC(2/6)
             2824  GAAGAC              
BsaHI          24  GGCGCC               G[GA]^CG[CT]C
             1027  GGCGTC              
BsgI          831  CTGCAC               GTGCAG(16/14)
             1033  CTGCAC              
BsmFI         361  GGGAC                GGGAC(10/14)
             3023  GGGAC               
BsmI         3789  GAATGC               GAATGC(1/-1)
             2939  GCATTC              
BspHI        2133  TCATGA               T^CATGA
             2306  TCATGA              
BssSI        2120  CACGAG               CACGAG(-5/-1)
             1256  CTCGTG              
BstBI        1973  TTCGAA               TT^CGAA
             2471  TTCGAA              
BstXI        1559  CCAAATTAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3585  CCATTTTGATGG        
DsaI          939  CCATGG               C^C[GA][CT]GG
             3598  CCATGG              
EaeI         2645  TGGCCA               [CT]^GGCC[GA]
             3146  TGGCCA              
FauI         1747  GCGGG                CCCGC(4/6)
             3477  GCGGG               
HaeII          24  GGCGCC               [GA]GCGC^[CT]
             3096  GGCGCT              
HgaI         2670  GACGC                GACGC(5/10)
             1028  GCGTC               
Hin1I          24  GGCGCC               G[GA]^CG[CT]C
             1027  GGCGTC              
MaeIII       2111  GTTAC                ^GT[ACGT]AC
             3400  GTTAC               
MfeI           89  CAATTG               C^AATTG
              386  CAATTG              
MluNI        2645  TGGCCA               TGG^CCA
             3146  TGGCCA              
MscI         2645  TGGCCA               TGG^CCA
             3146  TGGCCA              
MunI           89  CAATTG               C^AATTG
              386  CAATTG              
NcoI          939  CCATGG               C^CATGG
             3598  CCATGG              
NdeI          263  CATATG               CA^TATG
             1804  CATATG              
NspI         2147  ACATGT               [GA]CATG^[CT]
             2365  ACATGC              
NspV         1973  TTCGAA               TT^CGAA
             2471  TTCGAA              
PvuII        2962  CAGCTG               CAG^CTG
             3203  CAGCTG              
RcaI         2133  TCATGA               T^CATGA
             2306  TCATGA              
ScaI         1382  AGTACT               AGT^ACT
             1978  AGTACT              
SfuI         1973  TTCGAA               TT^CGAA
             2471  TTCGAA              


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AspHI       AvaII       
BanI        BanII       BbvI        BclI        BcnI        
BfaI        BmyI        BpmI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        EarI        EcoRII      EcoT14I     FbaI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PstI        
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     XhoII       XmnI        
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