Restriction map for pF1KA0389
 (Linear) (Six-base) MAPSORT of: KIAA0389.seq  Check: 5647  from: 1  to: 3855



KIAA0389.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:20  ..

Acc65I G'GTAC_C

Cuts at:      0    1224    3855 
   Size:       1224    2631
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     308     788    1058    1111    1486    2050    2462    2969 
   Size:        308     480     270      53     375     564     412     507

Cuts at:   2969    3769    3855 
   Size:        800      86

  Fragments arranged by size: 

                 800     564     507     480     412     375     308     270
                  86      53
 
AflII C'TTAA_G

Cuts at:      0     901    3855 
   Size:        901    2954
 
AflIII A'CryG_T

Cuts at:      0    2147    3855 
   Size:       2147    1708
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2473    3855 
   Size:       2473    1382
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2375    3208    3846    3855 
   Size:       2375     833     638       9

  Fragments arranged by size: 

                2375     833     638       9
 
ApaLI G'TGCA_C

Cuts at:      0     960    3855 
   Size:        960    2895
 
ApoI r'AATT_y

Cuts at:      0     254     484     622    1953    2051    2293    2554    2624 
   Size:        254     230     138    1331      98     242     261      70

Cuts at:   2624    3163    3310    3855 
   Size:        539     147     545

  Fragments arranged by size: 

                1331     545     539     261     254     242     230     147
                 138      98      70
 
AvaI C'yCGr_G

Cuts at:      0    2593    3855 
   Size:       2593    1262
 
BamHI G'GATC_C

Cuts at:      0    2187    3855 
   Size:       2187    1668
 
BanI G'GyrC_C

Cuts at:      0      24    1201    1224    3855 
   Size:         24    1177      23    2631

  Fragments arranged by size: 

                2631    1177      24      23
 
BanII G_rGCy'C

Cuts at:      0     655    3068    3290    3855 
   Size:        655    2413     222     565

  Fragments arranged by size: 

                2413     655     565     222
 
BbeI G_GCGC'C

Cuts at:      0      28    3855 
   Size:         28    3827
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     592    2831    3855 
   Size:        592    2239    1024

  Fragments arranged by size: 

                2239    1024     592
 
BbvCI CC'TCA_GC

Cuts at:      0    2776    3440    3855 
   Size:       2776     664     415

  Fragments arranged by size: 

                2776     664     415
 
BclI T'GATC_A

Cuts at:      0    1296    1617    2298    3855 
   Size:       1296     321     681    1557

  Fragments arranged by size: 

                1557    1296     681     321
 
BglI GCCn_nnn'nGGC

Cuts at:      0    3094    3855 
   Size:       3094     761
 
BglII A'GATC_T

Cuts at:      0    3728    3855 
   Size:       3728     127
 
Bme1580I G_kGCm'C

Cuts at:      0     964    1204    2533    3855 
   Size:        964     240    1329    1322

  Fragments arranged by size: 

                1329    1322     964     240
 
BmgBI CAC'GTC

Cuts at:      0    2818    3855 
   Size:       2818    1037
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1144    1767    3855 
   Size:       1144     623    2088

  Fragments arranged by size: 

                2088    1144     623
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     470    2003    3028    3855 
   Size:        470    1533    1025     827

  Fragments arranged by size: 

                1533    1025     827     470
 
Bpu10I CC'TnA_GC

Cuts at:      0    2776    3440    3855 
   Size:       2776     664     415

  Fragments arranged by size: 

                2776     664     415
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     406    2893    3712    3855 
   Size:        406    2487     819     143

  Fragments arranged by size: 

                2487     819     406     143
 
BsaAI yAC'GTr

Cuts at:      0    3256    3855 
   Size:       3256     599
 
BsaHI Gr'CG_yC

Cuts at:      0      25    1028    3855 
   Size:         25    1003    2827

  Fragments arranged by size: 

                2827    1003      25
 
BsaWI w'CCGG_w

Cuts at:      0    3135    3855 
   Size:       3135     720
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     656     668     686     698    3855 
   Size:        656      12      18      12    3157

  Fragments arranged by size: 

                3157     656      18      12      12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    2505    2716    2866    2995    3709    3855 
   Size:       2505     211     150     129     714     146

  Fragments arranged by size: 

                2505     714     211     150     146     129
 
BseYI C'CCAG_C

Cuts at:      0    1607    3450    3855 
   Size:       1607    1843     405

  Fragments arranged by size: 

                1843    1607     405
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     816    1018    3855 
   Size:        816     202    2837

  Fragments arranged by size: 

                2837     816     202
 
BsiHKAI G_wGCw'C

Cuts at:      0     422     655     964    2049    2398    3068    3290    3390 
   Size:        422     233     309    1085     349     670     222     100

Cuts at:   3390    3855 
   Size:        465

  Fragments arranged by size: 

                1085     670     465     422     349     309     233     222
                 100
 
BsmI GAATG_Cn'

Cuts at:      0    2939    3795    3855 
   Size:       2939     856      60

  Fragments arranged by size: 

                2939     856      60
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1709    3855 
   Size:       1709    2146
 
Bsp1286I G_dGCh'C

Cuts at:      0     422     655     964    1204    2049    2398    2533    3068 
   Size:        422     233     309     240     845     349     135     535

Cuts at:   3068    3290    3390    3855 
   Size:        222     100     465

  Fragments arranged by size: 

                 845     535     465     422     349     309     240     233
                 222     135     100
 
BspHI T'CATG_A

Cuts at:      0    2133    2306    3855 
   Size:       2133     173    1549

  Fragments arranged by size: 

                2133    1549     173
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     898    3855 
   Size:        898    2957
 
BsrDI GCAATG_nn'

Cuts at:      0    1434    3855 
   Size:       1434    2421
 
BsrFI r'CCGG_y

Cuts at:      0     463    3855 
   Size:        463    3392
 
BsrGI T'GTAC_A

Cuts at:      0    2150    3855 
   Size:       2150    1705
 
BssSI C'ACGA_G

Cuts at:      0    1256    2120    3855 
   Size:       1256     864    1735

  Fragments arranged by size: 

                1735    1256     864
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1252    3855 
   Size:       1252    2603
 
BstBI TT'CG_AA

Cuts at:      0    1974    2472    3855 
   Size:       1974     498    1383

  Fragments arranged by size: 

                1974    1383     498
 
BstEII G'GTnAC_C

Cuts at:      0    2110    3855 
   Size:       2110    1745
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1566    3592    3855 
   Size:       1566    2026     263

  Fragments arranged by size: 

                2026    1566     263
 
BstYI r'GATC_y

Cuts at:      0     701     921    2187    3728    3855 
   Size:        701     220    1266    1541     127

  Fragments arranged by size: 

                1541    1266     701     220     127
 
Bsu36I CC'TnA_GG

Cuts at:      0    3040    3855 
   Size:       3040     815
 
BtgI C'CryG_G

Cuts at:      0     939    3598    3855 
   Size:        939    2659     257

  Fragments arranged by size: 

                2659     939     257
 
BtsI GCAGTG_nn'

Cuts at:      0     214     293    1760    3855 
   Size:        214      79    1467    2095

  Fragments arranged by size: 

                2095    1467     214      79
 
DraI TTT'AAA

Cuts at:      0     203     821    2217    3855 
   Size:        203     618    1396    1638

  Fragments arranged by size: 

                1638    1396     618     203
 
EaeI y'GGCC_r

Cuts at:      0    2645    3146    3855 
   Size:       2645     501     709

  Fragments arranged by size: 

                2645     709     501
 
EarI CTCTTCn'nnn_

Cuts at:      0     149    1015    2753    2843    2900    3355    3855 
   Size:        149     866    1738      90      57     455     500

  Fragments arranged by size: 

                1738     866     500     455     149      90      57
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2877    3517    3855 
   Size:       2877     640     338

  Fragments arranged by size: 

                2877     640     338
 
EcoICRI GAG'CTC

Cuts at:      0     653    3066    3288    3855 
   Size:        653    2413     222     567

  Fragments arranged by size: 

                2413     653     567     222
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     308     470     788    1058    1111    1486    2003    2050 
   Size:        308     162     318     270      53     375     517      47

Cuts at:   2050    2462    2969    3028    3769    3855 
   Size:        412     507      59     741      86

  Fragments arranged by size: 

                 741     517     507     412     375     318     308     270
                 162      86      59      53      47
 
EcoO109I rG'GnC_Cy

Cuts at:      0    3178    3855 
   Size:       3178     677
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1018    1171    3855 
   Size:       1018     153    2684

  Fragments arranged by size: 

                2684    1018     153
 
HaeII r_GCGC'y

Cuts at:      0      28    3100    3855 
   Size:         28    3072     755

  Fragments arranged by size: 

                3072     755      28
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2601    2633    2971    3003    3162    3194    3855 
   Size:       2601      32     338      32     159      32     661

  Fragments arranged by size: 

                2601     661     338     159      32      32      32
 
HincII GTy'rAC

Cuts at:      0    2258    3855 
   Size:       2258    1597
 
HindIII A'AGCT_T

Cuts at:      0     398    1000    1989    2219    3855 
   Size:        398     602     989     230    1636

  Fragments arranged by size: 

                1636     989     602     398     230
 
HpaI GTT'AAC

Cuts at:      0    2258    3855 
   Size:       2258    1597
 
KasI G'GCGC_C

Cuts at:      0      24    3855 
   Size:         24    3831
 
KpnI G_GTAC'C

Cuts at:      0    1228    3855 
   Size:       1228    2627
 
MfeI C'AATT_G

Cuts at:      0      89     386    3855 
   Size:         89     297    3469

  Fragments arranged by size: 

                3469     297      89
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     642    3855 
   Size:        642    3213
 
MscI TGG'CCA

Cuts at:      0    2647    3148    3855 
   Size:       2647     501     707

  Fragments arranged by size: 

                2647     707     501
 
MslI CAynn'nnrTG

Cuts at:      0     415     641     967    1924    2455    2665    3590    3673 
   Size:        415     226     326     957     531     210     925      83

Cuts at:   3673    3855 
   Size:        182

  Fragments arranged by size: 

                 957     925     531     415     326     226     210     182
                  83
 
MspA1I CmG'CkG

Cuts at:      0    2964    3205    3477    3855 
   Size:       2964     241     272     378

  Fragments arranged by size: 

                2964     378     272     241
 
NaeI GCC'GGC

Cuts at:      0     465    3855 
   Size:        465    3390
 
NarI GG'CG_CC

Cuts at:      0      25    3855 
   Size:         25    3830
 
NcoI C'CATG_G

Cuts at:      0     939    3598    3855 
   Size:        939    2659     257

  Fragments arranged by size: 

                2659     939     257
 
NdeI CA'TA_TG

Cuts at:      0     264    1805    3855 
   Size:        264    1541    2050

  Fragments arranged by size: 

                2050    1541     264
 
NgoMIV G'CCGG_C

Cuts at:      0     463    3855 
   Size:        463    3392
 
NspI r_CATG'y

Cuts at:      0    2151    2369    3855 
   Size:       2151     218    1486

  Fragments arranged by size: 

                2151    1486     218
 
PciI A'CATG_T

Cuts at:      0    2147    3855 
   Size:       2147    1708
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1779    3855 
   Size:       1779    2076
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1466    1498    3855 
   Size:       1466      32    2357

  Fragments arranged by size: 

                2357    1466      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    3178    3855 
   Size:       3178     677
 
PsiI TTA'TAA

Cuts at:      0    1219    3855 
   Size:       1219    2636
 
PstI C_TGCA'G

Cuts at:      0     153    1638    3842    3855 
   Size:        153    1485    2204      13

  Fragments arranged by size: 

                2204    1485     153      13
 
PvuII CAG'CTG

Cuts at:      0    2964    3205    3855 
   Size:       2964     241     650

  Fragments arranged by size: 

                2964     650     241
 
SacI G_AGCT'C

Cuts at:      0     655    3068    3290    3855 
   Size:        655    2413     222     565

  Fragments arranged by size: 

                2413     655     565     222
 
ScaI AGT'ACT

Cuts at:      0    1384    1980    3855 
   Size:       1384     596    1875

  Fragments arranged by size: 

                1875    1384     596
 
SfcI C'TryA_G

Cuts at:      0      35     149    1634    3838    3855 
   Size:         35     114    1485    2204      17

  Fragments arranged by size: 

                2204    1485     114      35      17
 
SfoI GGC'GCC

Cuts at:      0      26    3855 
   Size:         26    3829
 
SmlI C'TyrA_G

Cuts at:      0     421     901    2872    3691    3855 
   Size:        421     480    1971     819     164

  Fragments arranged by size: 

                1971     819     480     421     164
 
SspI AAT'ATT

Cuts at:      0      60    1047    1120    3855 
   Size:         60     987      73    2735

  Fragments arranged by size: 

                2735     987      73      60
 
StyI C'CwwG_G

Cuts at:      0     939    1038    3598    3855 
   Size:        939      99    2560     257

  Fragments arranged by size: 

                2560     939     257      99
 
SwaI ATTT'AAAT

Cuts at:      0     203    3855 
   Size:        203    3652
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3654    3855 
   Size:       3654     201
 
TatI w'GTAC_w

Cuts at:      0    1382    1978    2150    3186    3542    3855 
   Size:       1382     596     172    1036     356     313

  Fragments arranged by size: 

                1382    1036     596     356     313     172
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     668     700    3705    3737    3855 
   Size:        668      32    3005      32     118

  Fragments arranged by size: 

                3005     668     118      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    3648    3855 
   Size:       3648     207
 
XmnI GAAnn'nnTTC

Cuts at:      0     785    1436    1460    3311    3855 
   Size:        785     651      24    1851     544

  Fragments arranged by size: 

                1851     785     651     544      24
 

 Enzymes that do cut:

   Acc65I     AcuI    AflII   AflIII     AlfI    AlwNI    ApaLI     ApoI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BclI
     BglI    BglII Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI
     BtsI     DraI     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
     FalI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NspI     PciI    PflMI     PpiI    PpuMI     PsiI
     PstI    PvuII     SacI     ScaI     SfcI     SfoI     SmlI     SspI
     StyI     SwaI    TaqII     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI     AgeI     AhdI     AleI
     AloI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI     BcgI
    BciVI    BfrBI     BlpI     BmtI     BplI     BsaI    BsaBI    BsiEI
    BsiWI    BspEI    BsrBI   BssHII  BstZ17I    BtgZI     ClaI    CspCI
   DraIII     DrdI     EagI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI     MluI     NheI     NotI     NruI     NsiI     PacI     PasI
     PfoI     PmeI     PmlI    PshAI   PspOMI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI
  Tth111I     XbaI     XhoI     XmaI     ZraI
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