Restriction map for pF1KA0361
##################################
# NO CUT ENZYMES (   74 enzymes) #
##################################

AatII       Acc65I      AflII       AgeI        AscI        
AseI        AsnI        Asp718I     AspI        AvrII       
BamHI       BcgI        BfrI        BglII       BlnI        
BsaBI       BsiWI       Bsp1407I    BspDI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     ClaI        CpoI        DraI        
EagI        EclXI       Eco52I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        HpaI        KpnI        
MamI        MluI        MseI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshBI       
RsrII       SalI        SbfI        ScaI        SfiI        
SfuI        SgrAI       SmaI        SpeI        Sse8387I    
SspBI       SspI        SwaI        Tru9I       Tth111I     
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI         1366  GTCTAC               GT^[AC][GT]AC
AclI         1745  AACGTT               AA^CGTT
AflIII        906  ACATGT               A^C[GA][CT]GT
AhdI          905  GACATGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I      1914  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         905  GACATGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        3693  TGCGCA               TGC^GCA
BbeI         3670  GGCGCC               GGCGC^C
BbsI          913  GTCTTC               GAAGAC(2/6)
BciVI        1110  GTATCC               GTATCC(6/5)
BfaI          598  CTAG                 C^TAG
BpuAI         913  GTCTTC               GAAGAC(2/6)
BsmBI        3037  GAGACG               CGTCTC(1/5)
BsmI         1647  GAATGC               GAATGC(1/-1)
BspLU11I      906  ACATGT               A^CATGT
BssSI        2854  CTCGTG               CACGAG(-5/-1)
BstYI        1095  GGATCT               [GA]^GATC[CT]
DraIII       1024  CACGTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         2203  GACCCAAAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      905  GACATGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1691  CTCTTC               CTCTTC(1/4)
FspI         3693  TGCGCA               TGC^GCA
HgaI          104  GCGTC                GACGC(5/10)
HincII       3482  GTCAAC               GT[CT]^[GA]AC
HindII       3482  GTCAAC               GT[CT]^[GA]AC
HindIII      1101  AAGCTT               A^AGCTT
KasI         3670  GGCGCC               G^GCGCC
Ksp632I      1691  CTCTTC               CTCTTC(1/4)
KspI         1014  CCGCGG               CCGC^GG
MaeI          598  CTAG                 C^TAG
MfeI         3305  CAATTG               C^AATTG
MflI         1095  GGATCT               [GA]^GATC[CT]
MunI         3305  CAATTG               C^AATTG
NarI         3670  GGCGCC               GG^CGCC
PshAI        1725  GACTCATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     1745  AACGTT               AA^CGTT
PvuI         1806  CGATCG               CGAT^CG
SacII        1014  CCGCGG               CCGC^GG
SapI         1690  GCTCTTC              GCTCTTC(1/4)
SexAI        2249  ACCTGGT              A^CC[AT]GGT
SfoI         3670  GGCGCC               GGC^GCC
SmlI         2524  CTCAAG               C^T[CT][GA]AG
SnaBI        3709  TACGTA               TAC^GTA
SphI          494  GCATGC               GCATG^C
XhoII        1095  GGATCT               [GA]^GATC[CT]
XmnI         1914  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

AccII         325  CGCG                 CG^CG
             1015  CGCG                
AccIII       3437  TCCGGA               T^CCGGA
             3726  TCCGGA              
AcsI          802  AAATTC               [GA]^AATT[CT]
             1119  GAATTT              
AcyI          103  GGCGTC               G[GA]^CG[CT]C
             3670  GGCGCC              
AfaI         1674  GTAC                 GT^AC
             3807  GTAC                
AocI          856  CCTGAGG              CC^T[ACGT]AGG
             3102  CCTGAGG             
Aor51HI       614  AGCGCT               AGC^GCT
             2030  AGCGCT              
ApoI          802  AAATTC               [GA]^AATT[CT]
             1119  GAATTT              
BanI         1768  GGCACC               G^G[CT][GA]CC
             3670  GGCGCC              
BbrPI         718  CACGTG               CAC^GTG
             1024  CACGTG              
BclI         1493  TGATCA               T^GATCA
             1545  TGATCA              
BlpI         1974  GCTGAGC              GC^T[ACGT]AGC
             3421  GCTGAGC             
Bpu1102I     1974  GCTGAGC              GC^T[ACGT]AGC
             3421  GCTGAGC             
BsaHI         103  GGCGTC               G[GA]^CG[CT]C
             3670  GGCGCC              
BseAI        3437  TCCGGA               T^CCGGA
             3726  TCCGGA              
BsiEI         438  CGACCG               CG[GA][CT]^CG
             1806  CGATCG              
BspEI        3437  TCCGGA               T^CCGGA
             3726  TCCGGA              
BspHI         761  TCATGA               T^CATGA
             1542  TCATGA              
BspMI         254  ACCTGC               ACCTGC(4/8)
             1407  GCAGGT              
BstUI         325  CGCG                 CG^CG
             1015  CGCG                
Bsu36I        856  CCTGAGG              CC^T[ACGT]AGG
             3102  CCTGAGG             
CelII        1974  GCTGAGC              GC^T[ACGT]AGC
             3421  GCTGAGC             
Eco47III      614  AGCGCT               AGC^GCT
             2030  AGCGCT              
Eco81I        856  CCTGAGG              CC^T[ACGT]AGG
             3102  CCTGAGG             
EcoNI        2880  CCTGGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3027  CCTCTGGGAGG         
FbaI         1493  TGATCA               T^GATCA
             1545  TGATCA              
Hin1I         103  GGCGTC               G[GA]^CG[CT]C
             3670  GGCGCC              
MroI         3437  TCCGGA               T^CCGGA
             3726  TCCGGA              
MvnI          325  CGCG                 CG^CG
             1015  CGCG                
NaeI          539  GCCGGC               GCC^GGC
             1042  GCCGGC              
NcoI         1295  CCATGG               C^CATGG
             2963  CCATGG              
NgoMIV        539  GCCGGC               G^CCGGC
             1042  GCCGGC              
PmaCI         718  CACGTG               CAC^GTG
             1024  CACGTG              
PmlI          718  CACGTG               CAC^GTG
             1024  CACGTG              
RcaI          761  TCATGA               T^CATGA
             1542  TCATGA              
RsaI         1674  GTAC                 GT^AC
             3807  GTAC                
SacI         1882  GAGCTC               GAGCT^C
             2149  GAGCTC              


#########################################
# 3 OR MORE CUT ENZYMES (  101 enzymes) #
#########################################

AciI        AluI        Alw44I      AlwI        AlwNI       
ApaI        ApaLI       AspHI       AvaI        AvaII       
BalI        BanII       BbvCI       BbvI        BcnI        
BglI        BmyI        BpmI        BsaAI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrBI       BsrDI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstXI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StuI        StyI        TaqI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        
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