Restriction map for pF1KA0361
 (Linear) (Six-base) MAPSORT of: KIAA0361.seq  Check: 1201  from: 1  to: 4014



KIAA0361.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1398    4014 
   Size:       1398    2616
 
AccI GT'mk_AC

Cuts at:      0    1367    4014 
   Size:       1367    2647
 
AclI AA'CG_TT

Cuts at:      0    1746    4014 
   Size:       1746    2268
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2555    2658    2914    3333    3441    4014 
   Size:       2555     103     256     419     108     573

  Fragments arranged by size: 

                2555     573     419     256     108     103
 
AfeI AGC'GCT

Cuts at:      0     616    2032    4014 
   Size:        616    1416    1982

  Fragments arranged by size: 

                1982    1416     616
 
AflIII A'CryG_T

Cuts at:      0     906    4014 
   Size:        906    3108
 
AhdI GACnn_n'nnGTC

Cuts at:      0     910    4014 
   Size:        910    3104
 
AlwNI CAG_nnn'CTG

Cuts at:      0      55     735    1956    1992    2509    2691    2892    3394 
   Size:         55     680    1221      36     517     182     201     502

Cuts at:   3394    4014 
   Size:        620

  Fragments arranged by size: 

                1221     680     620     517     502     201     182      55
                  36
 
ApaI G_GGCC'C

Cuts at:      0     275    1023    2099    2957    3122    3547    4014 
   Size:        275     748    1076     858     165     425     467

  Fragments arranged by size: 

                1076     858     748     467     425     275     165
 
ApaLI G'TGCA_C

Cuts at:      0     739    1005    1981    4014 
   Size:        739     266     976    2033

  Fragments arranged by size: 

                2033     976     739     266
 
ApoI r'AATT_y

Cuts at:      0     802    1119    4014 
   Size:        802     317    2895

  Fragments arranged by size: 

                2895     802     317
 
AvaI C'yCGr_G

Cuts at:      0     221     639    2429    2587    3188    4014 
   Size:        221     418    1790     158     601     826

  Fragments arranged by size: 

                1790     826     601     418     221     158
 
BanI G'GyrC_C

Cuts at:      0    1768    3670    4014 
   Size:       1768    1902     344

  Fragments arranged by size: 

                1902    1768     344
 
BanII G_rGCy'C

Cuts at:      0     275    1023    1886    2099    2153    2457    2572    2957 
   Size:        275     748     863     213      54     304     115     385

Cuts at:   2957    3027    3122    3373    3547    4014 
   Size:         70      95     251     174     467

  Fragments arranged by size: 

                 863     748     467     385     304     275     251     213
                 174     115      95      70      54
 
BbeI G_GCGC'C

Cuts at:      0    3674    4014 
   Size:       3674     340
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     906    4014 
   Size:        906    3108
 
BbvCI CC'TCA_GC

Cuts at:      0     143     161     404    1699    2416    2840    3427    4014 
   Size:        143      18     243    1295     717     424     587     587

  Fragments arranged by size: 

                1295     717     587     587     424     243     143      18
            
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1121    4014 
   Size:       1121    2893
 
BclI T'GATC_A

Cuts at:      0    1493    1545    4014 
   Size:       1493      52    2469

  Fragments arranged by size: 

                2469    1493      52
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1971    2224    2356    2942    3564    4014 
   Size:       1971     253     132     586     622     450

  Fragments arranged by size: 

                1971     622     586     450     253     132
 
BlpI GC'TnA_GC

Cuts at:      0    1975    3422    4014 
   Size:       1975    1447     592

  Fragments arranged by size: 

                1975    1447     592
 
Bme1580I G_kGCm'C

Cuts at:      0     275     743    1009    1023    1740    1771    1985    2099 
   Size:        275     468     266      14     717      31     214     114

Cuts at:   2099    2596    2957    3122    3164    3547    4014 
   Size:        497     361     165      42     383     467

  Fragments arranged by size: 

                 717     497     468     467     383     361     275     266
                 214     165     114      42      31      14
 
BmgBI CAC'GTC

Cuts at:      0    3276    4014 
   Size:       3276     738
 
BmrI ACTGGGnnnn_n'

Cuts at:      0       1     149     341     538    2601    3783    4014 
   Size:          1     148     192     197    2063    1182     231

  Fragments arranged by size: 

                2063    1182     231     197     192     148       1
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     571    1156    1213    1619    1799    1900    2014 
   Size:        283     288     585      57     406     180     101     114

Cuts at:   2014    2621    2779    2881    2947    3019    3952    4014 
   Size:        607     158     102      66      72     933      62

  Fragments arranged by size: 

                 933     607     585     406     288     283     180     158
                 114     102     101      72      66      62      57
 
Bpu10I CC'TnA_GC

Cuts at:      0     143     161     404    1699    2416    2715    2840    3427 
   Size:        143      18     243    1295     717     299     125     587

Cuts at:   3427    3893    4014 
   Size:        466     121

  Fragments arranged by size: 

                1295     717     587     466     299     243     143     125
                 121      18
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2509    4014 
   Size:       2509    1505
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     352    1454    1775    2437    3218    4014 
   Size:        352    1102     321     662     781     796

  Fragments arranged by size: 

                1102     796     781     662     352     321
 
BsaAI yAC'GTr

Cuts at:      0     720    1026    3711    4014 
   Size:        720     306    2685     303

  Fragments arranged by size: 

                2685     720     306     303
 
BsaHI Gr'CG_yC

Cuts at:      0     104    3671    4014 
   Size:        104    3567     343

  Fragments arranged by size: 

                3567     343     104
 
BsaWI w'CCGG_w

Cuts at:      0     440    1784    3437    3726    4014 
   Size:        440    1344    1653     289     288

  Fragments arranged by size: 

                1653    1344     440     289     288
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1349    1379    2057    2087    2729    2759    4014 
   Size:       1349      30     678      30     642      30    1255

  Fragments arranged by size: 

                1349    1255     678     642      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     178    1472    2099    2161    2698    2857    3016    3049 
   Size:        178    1294     627      62     537     159     159      33

Cuts at:   3049    3103    3223    4014 
   Size:         54     120     791

  Fragments arranged by size: 

                1294     791     627     537     178     159     159     120
                  62      54      33
 
BseYI C'CCAG_C

Cuts at:      0     336    1593    1896    2078    2618    2931    3121    3181 
   Size:        336    1257     303     182     540     313     190      60

Cuts at:   3181    3256    3415    3503    3554    3614    4014 
   Size:         75     159      88      51      60     400

  Fragments arranged by size: 

                1257     540     400     336     313     303     190     182
                 159      88      75      60      60      51
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     418    1426    1432    1471    2887    3754    4014 
   Size:        418    1008       6      39    1416     867     260

  Fragments arranged by size: 

                1416    1008     867     418     260      39       6
 
BsiEI CG_ry'CG

Cuts at:      0     441    1809    4014 
   Size:        441    1368    2205

  Fragments arranged by size: 

                2205    1368     441
 
BsiHKAI G_wGCw'C

Cuts at:      0     646     686     743     769    1009    1886    1985    2153 
   Size:        646      40      57      26     240     877      99     168

Cuts at:   2153    4014 
   Size:       1861

  Fragments arranged by size: 

                1861     877     646     240     168      99      57      40
                  26
 
BsmI GAATG_Cn'

Cuts at:      0    1653    4014 
   Size:       1653    2361
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3031    4014 
   Size:       3031     983
 
Bsp1286I G_dGCh'C

Cuts at:      0     275     646     686     743     769    1009    1023    1740 
   Size:        275     371      40      57      26     240      14     717

Cuts at:   1740    1771    1886    1985    2099    2153    2457    2572    2596 
   Size:         31     115      99     114      54     304     115      24

Cuts at:   2596    2957    3027    3122    3164    3373    3547    4014 
   Size:        361      70      95      42     209     174     467

  Fragments arranged by size: 

                 717     467     371     361     304     275     240     209
                 174     115     115     114      99      95      70      57
                  54      42      40      31      26      24      14
 
BspEI T'CCGG_A

Cuts at:      0    3437    3726    4014 
   Size:       3437     289     288

  Fragments arranged by size: 

                3437     289     288
 
BspHI T'CATG_A

Cuts at:      0     761    1542    4014 
   Size:        761     781    2472

  Fragments arranged by size: 

                2472     781     761
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     263    1398    4014 
   Size:        263    1135    2616

  Fragments arranged by size: 

                2616    1135     263
 
BsrBI CCG'CTC

Cuts at:      0    1217    2057    3111    3897    4014 
   Size:       1217     840    1054     786     117

  Fragments arranged by size: 

                1217    1054     840     786     117
 
BsrDI GCAATG_nn'

Cuts at:      0     416    1569    1575    2919    4014 
   Size:        416    1153       6    1344    1095

  Fragments arranged by size: 

                1344    1153    1095     416       6
 
BsrFI r'CCGG_y

Cuts at:      0     374     539    1042    2582    3056    4014 
   Size:        374     165     503    1540     474     958

  Fragments arranged by size: 

                1540     958     503     474     374     165
 
BssSI C'ACGA_G

Cuts at:      0    2854    4014 
   Size:       2854    1160
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     336     423     571     724    1114    1434    2049    2172 
   Size:        336      87     148     153     390     320     615     123

Cuts at:   2172    3256    3759    3773    4014 
   Size:       1084     503      14     241

  Fragments arranged by size: 

                1084     615     503     390     336     320     241     153
                 148     123      87      14
 
BstYI r'GATC_y

Cuts at:      0    1095    4014 
   Size:       1095    2919
 
Bsu36I CC'TnA_GG

Cuts at:      0     857    3103    4014 
   Size:        857    2246     911

  Fragments arranged by size: 

                2246     911     857
 
BtgI C'CryG_G

Cuts at:      0    1014    1295    2018    2060    2228    2963    3324    4014 
   Size:       1014     281     723      42     168     735     361     690

  Fragments arranged by size: 

                1014     735     723     690     361     281     168      42
            
 
BtsI GCAGTG_nn'

Cuts at:      0     825    1037    2092    2375    2399    3766    3922    4014 
   Size:        825     212    1055     283      24    1367     156      92

  Fragments arranged by size: 

                1367    1055     825     283     212     156      92      24
            
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1853    1888    4014 
   Size:       1853      35    2126

  Fragments arranged by size: 

                2126    1853      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1029    4014 
   Size:       1029    2985
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2209    4014 
   Size:       2209    1805
 
EaeI y'GGCC_r

Cuts at:      0      36     871    1242    1833    2021    2225    2231    3236 
   Size:         36     835     371     591     188     204       6    1005

Cuts at:   3236    3445    3565    3864    4014 
   Size:        209     120     299     150

  Fragments arranged by size: 

                1005     835     591     371     299     209     204     188
                 150     120      36       6
 
EarI CTCTTCn'nnn_

Cuts at:      0    1697    4014 
   Size:       1697    2317
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2359    4014 
   Size:       2359    1655
 
EcoICRI GAG'CTC

Cuts at:      0    1884    2151    4014 
   Size:       1884     267    1863

  Fragments arranged by size: 

                1884    1863     267
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     571    1156    1213    1619    1799    1900    2014 
   Size:        283     288     585      57     406     180     101     114

Cuts at:   2014    2555    2621    2658    2779    2881    2914    2947    3019 
   Size:        541      66      37     121     102      33      33      72

Cuts at:   3019    3333    3441    3952    4014 
   Size:        314     108     511      62

  Fragments arranged by size: 

                 585     541     511     406     314     288     283     180
                 121     114     108     102     101      72      66      62
                  57      37      33      33
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2884    3031    4014 
   Size:       2884     147     983

  Fragments arranged by size: 

                2884     983     147
 
EcoO109I rG'GnC_Cy

Cuts at:      0     147     340     594     861    1019    1323    1360    1512 
   Size:        147     193     254     267     158     304      37     152

Cuts at:   1512    1635    1703    2095    2096    2954    3099    3118    3288 
   Size:        123      68     392       1     858     145      19     170

Cuts at:   3288    3544    3634    3827    4014 
   Size:        256      90     193     187

  Fragments arranged by size: 

                 858     392     304     267     256     254     193     193
                 187     170     158     152     147     145     123      90
                  68      37      19       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1938    4014 
   Size:       1938    2076
 
FspI TGC'GCA

Cuts at:      0    3695    4014 
   Size:       3695     319
 
FspAI rTGC'GCAy

Cuts at:      0    3695    4014 
   Size:       3695     319
 
HaeII r_GCGC'y

Cuts at:      0     372     618    1556    2034    3435    3603    3674    4014 
   Size:        372     246     938     478    1401     168      71     340

  Fragments arranged by size: 

                1401     938     478     372     340     246     168      71
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     896     928    1349    1381    2532    2564    2729    2761 
   Size:        896      32     421      32    1151      32     165      32

Cuts at:   2761    2809    2841    3182    3214    3280    3312    3390    3422 
   Size:         48      32     341      32      66      32      78      32

Cuts at:   3422    4014 
   Size:        592

  Fragments arranged by size: 

                1151     896     592     421     341     165      78      66
                  48      32      32      32      32      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    3484    4014 
   Size:       3484     530
 
HindIII A'AGCT_T

Cuts at:      0    1101    4014 
   Size:       1101    2913
 
KasI G'GCGC_C

Cuts at:      0    3670    4014 
   Size:       3670     344
 
MfeI C'AATT_G

Cuts at:      0    3305    4014 
   Size:       3305     709
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1335    1362    4014 
   Size:       1335      27    2652

  Fragments arranged by size: 

                2652    1335      27
 
MscI TGG'CCA

Cuts at:      0      38    1835    2023    4014 
   Size:         38    1797     188    1991

  Fragments arranged by size: 

                1991    1797     188      38
 
MslI CAynn'nnrTG

Cuts at:      0      20     722    1733    4014 
   Size:         20     702    1011    2281

  Fragments arranged by size: 

                2281    1011     702      20
 
MspA1I CmG'CkG

Cuts at:      0     142     403     633    1016    1248    1597    3051    3391 
   Size:        142     261     230     383     232     349    1454     340

Cuts at:   3391    4014 
   Size:        623

  Fragments arranged by size: 

                1454     623     383     349     340     261     232     230
                 142
 
NaeI GCC'GGC

Cuts at:      0     541    1044    4014 
   Size:        541     503    2970

  Fragments arranged by size: 

                2970     541     503
 
NarI GG'CG_CC

Cuts at:      0    3671    4014 
   Size:       3671     343
 
NcoI C'CATG_G

Cuts at:      0    1295    2963    4014 
   Size:       1295    1668    1051

  Fragments arranged by size: 

                1668    1295    1051
 
NgoMIV G'CCGG_C

Cuts at:      0     539    1042    4014 
   Size:        539     503    2972

  Fragments arranged by size: 

                2972     539     503
 
NspI r_CATG'y

Cuts at:      0     498     910    2753    4014 
   Size:        498     412    1843    1261

  Fragments arranged by size: 

                1843    1261     498     412
 
PasI CC'CwG_GG

Cuts at:      0    1088    1969    2323    2382    2572    2810    3410    3832 
   Size:       1088     881     354      59     190     238     600     422

Cuts at:   3832    4014 
   Size:        182

  Fragments arranged by size: 

                1088     881     600     422     354     238     190     182
                  59
 
PciI A'CATG_T

Cuts at:      0     906    4014 
   Size:        906    3108
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1029    1992    2925    2964    3383    4014 
   Size:       1029     963     933      39     419     631

  Fragments arranged by size: 

                1029     963     933     631     419      39
 
PfoI T'CCnGG_A

Cuts at:      0     620    2276    4014 
   Size:        620    1656    1738

  Fragments arranged by size: 

                1738    1656     620
 
PmlI CAC'GTG

Cuts at:      0     720    1026    4014 
   Size:        720     306    2988

  Fragments arranged by size: 

                2988     720     306
 
PpuMI rG'GwC_Cy

Cuts at:      0     594     861    1360    1635    1703    3288    3827    4014 
   Size:        594     267     499     275      68    1585     539     187

  Fragments arranged by size: 

                1585     594     539     499     275     267     187      68
            
 
PshAI GACnn'nnGTC

Cuts at:      0    1729    4014 
   Size:       1729    2285
 
PspOMI G'GGCC_C

Cuts at:      0     271    1019    2095    2953    3118    3543    4014 
   Size:        271     748    1076     858     165     425     471

  Fragments arranged by size: 

                1076     858     748     471     425     271     165
 
PstI C_TGCA'G

Cuts at:      0     101    1537    1931    1952    3537    4014 
   Size:        101    1436     394      21    1585     477

  Fragments arranged by size: 

                1585    1436     477     394     101      21
 
PvuI CG_AT'CG

Cuts at:      0    1809    4014 
   Size:       1809    2205
 
PvuII CAG'CTG

Cuts at:      0     142     403    1597    3051    3391    4014 
   Size:        142     261    1194    1454     340     623

  Fragments arranged by size: 

                1454    1194     623     340     261     142
 
SacI G_AGCT'C

Cuts at:      0    1886    2153    4014 
   Size:       1886     267    1861

  Fragments arranged by size: 

                1886    1861     267
 
SacII CC_GC'GG

Cuts at:      0    1017    4014 
   Size:       1017    2997
 
SanDI GG'GwC_CC

Cuts at:      0    1635    3827    4014 
   Size:       1635    2192     187

  Fragments arranged by size: 

                2192    1635     187
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1697    4014 
   Size:       1697    2317
 
SexAI A'CCwGG_T

Cuts at:      0    2249    4014 
   Size:       2249    1765
 
SfcI C'TryA_G

Cuts at:      0      97     628    1368    1533    1927    1948    2788    3061 
   Size:         97     531     740     165     394      21     840     273

Cuts at:   3061    3533    4014 
   Size:        472     481

  Fragments arranged by size: 

                 840     740     531     481     472     394     273     165
                  97      21
 
SfoI GGC'GCC

Cuts at:      0    3672    4014 
   Size:       3672     342
 
SmlI C'TyrA_G

Cuts at:      0    2524    4014 
   Size:       2524    1490
 
SnaBI TAC'GTA

Cuts at:      0    3711    4014 
   Size:       3711     303
 
SphI G_CATG'C

Cuts at:      0     498    4014 
   Size:        498    3516
 
StuI AGG'CCT

Cuts at:      0     655    2069    2741    2852    3572    3909    4014 
   Size:        655    1414     672     111     720     337     105

  Fragments arranged by size: 

                1414     720     672     655     337     111     105
 
StyI C'CwwG_G

Cuts at:      0    1220    1295    1517    2171    2963    3910    4014 
   Size:       1220      75     222     654     792     947     104

  Fragments arranged by size: 

                1220     947     792     654     222     104      75
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     520     784    1881    2374    3495    4014 
   Size:        520     264    1097     493    1121     519

  Fragments arranged by size: 

                1121    1097     520     519     493     264
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2057    2089    2272    2304    4014 
   Size:       2057      32     183      32    1710

  Fragments arranged by size: 

                2057    1710     183      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1030    3592    3627    4014 
   Size:       1030    2562      35     387

  Fragments arranged by size: 

                2562    1030     387      35
 
XmnI GAAnn'nnTTC

Cuts at:      0    1918    4014 
   Size:       1918    2096
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI     AfeI   AflIII     AhdI    AlwNI
     ApaI    ApaLI     ApoI     AvaI     BanI    BanII     BbeI     BbsI
    BbvCI    BciVI     BclI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI    BstXI
    BstYI   Bsu36I     BtgI     BtsI    CspCI   DraIII     DrdI     EaeI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     KasI     MfeI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI     PciI    PflMI     PfoI     PmlI    PpuMI    PshAI   PspOMI
     PstI     PvuI    PvuII     SacI    SacII    SanDI     SapI    SexAI
     SfcI     SfoI     SmlI    SnaBI     SphI     StuI     StyI    TaqII
     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I    AflII     AgeI     AleI     AlfI     AloI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BcgI    BfrBI    BglII
     BmtI     BplI    BsaBI    BsiWI    BsrGI   BssHII   BstAPI    BstBI
   BstEII  BstZ17I    BtgZI     ClaI     DraI     EagI    EcoRI    EcoRV
     FseI     HpaI     KpnI     MluI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PmeI     PpiI     PsiI    PspXI     PsrI    RsrII
     SalI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmaI     SpeI
     SrfI     SspI     SwaI     TatI  Tth111I     XbaI     XhoI     XmaI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com