Restriction map for pF1KA0182
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        ApoI        AscI        AseI        
AsnI        Asp718I     AspI        AviII       BamHI       
BciVI       BclI        BfrI        BglII       BsaBI       
BseAI       BsiEI       BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrGI       
BssHII      Bst1107I    BstYI       BstZ17I     ClaI        
CpoI        DraI        EagI        EclXI       Eco52I      
EcoRI       EcoT22I     FbaI        FspI        HpaI        
KpnI        MamI        MfeI        MflI        MluI        
MroI        MseI        MunI        NdeI        NotI        
NruI        NsiI        PacI        PflFI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SalI        ScaI        SexAI       
SgrAI       SnaBI       SpeI        SspBI       SspI        
StuI        SwaI        Tru9I       Tth111I     XbaI        
XhoII       


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AhdI         1309  GACCGGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        394  GTGCAC               G^TGCAC
ApaLI         394  GTGCAC               G^TGCAC
Asp700I      2370  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1309  GACCGGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        3403  CCTAGG               C^CTAGG
BalI         1035  TGGCCA               TGG^CCA
BbeI         1119  GGCGCC               GGCGC^C
BbrPI         400  CACGTG               CAC^GTG
BbsI         1663  GTCTTC               GAAGAC(2/6)
BcgI          668  CGATGCACCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI         3403  CCTAGG               C^CTAGG
BpuAI        1663  GTCTTC               GAAGAC(2/6)
BsaAI         400  CACGTG               [CT]AC^GT[GA]
BsaI         3327  GGTCTC               GGTCTC(1/5)
BsaWI        1149  TCCGGT               [AT]^CCGG[AT]
BsrDI        3391  GCAATG               GCAATG(2/0)
BstBI        2359  TTCGAA               TT^CGAA
BstEII        150  GGTGACC              G^GT[ACGT]ACC
BstPI         150  GGTGACC              G^GT[ACGT]ACC
Eam1105I     1309  GACCGGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1857  CCTCCCACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       150  GGTGACC              G^GT[ACGT]ACC
EcoRV        3422  GATATC               GAT^ATC
FseI         1369  GGCCGGCC             GGCCGG^CC
HincII       2950  GTTGAC               GT[CT]^[GA]AC
HindII       2950  GTTGAC               GT[CT]^[GA]AC
KasI         1119  GGCGCC               G^GCGCC
KspI         2618  CCGCGG               CCGC^GG
MluNI        1035  TGGCCA               TGG^CCA
MscI         1035  TGGCCA               TGG^CCA
MslI         2778  CATTCCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI         1119  GGCGCC               GG^CGCC
NheI         2676  GCTAGC               G^CTAGC
NspV         2359  TTCGAA               TT^CGAA
PaeR7I       1813  CTCGAG               C^TCGAG
PmaCI         400  CACGTG               CAC^GTG
PmlI          400  CACGTG               CAC^GTG
PvuII        1072  CAGCTG               CAG^CTG
SacII        2618  CCGCGG               CCGC^GG
SbfI         1113  CCTGCAGG             CCTGCA^GG
SfiI          978  GGCCGCCAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1119  GGCGCC               GGC^GCC
SfuI         2359  TTCGAA               TT^CGAA
SmlI         1813  CTCGAG               C^T[CT][GA]AG
SphI         2741  GCATGC               GCATG^C
Sse8387I     1113  CCTGCAGG             CCTGCA^GG
TfiI          358  GATTC                G^A[AT]TC
XcmI         2796  CCACAGCACCAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1813  CTCGAG               C^TCGAG
XmnI         2370  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI         2316  GTCTAC               GT^[AC][GT]AC
             3233  GTAGAC              
AcyI         1119  GGCGCC               G[GA]^CG[CT]C
             2292  GACGCC              
AflIII       2141  ACACGT               A^C[GA][CT]GT
             2954  ACACGT              
AlwI         1242  GATCC                GGATC(4/5)
             3155  GGATC               
AocI          268  CCTCAGG              CC^T[ACGT]AGG
              606  CCTGAGG             
Aor51HI       729  AGCGCT               AGC^GCT
             2407  AGCGCT              
BbvCI         756  CCTCAGC              CCTCAGC(-5/-2)
             3261  CCTCAGC             
BglI          979  GCCGCCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1911  GCCCTACCGGC         
BsaHI        1119  GGCGCC               G[GA]^CG[CT]C
             2292  GACGCC              
BsmBI        2526  CGTCTC               CGTCTC(1/5)
             1567  GAGACG              
Bsu36I        268  CCTCAGG              CC^T[ACGT]AGG
              606  CCTGAGG             
DpnII        1242  GATC                 ^GATC
             3156  GATC                
DrdI         1427  GACCAAACCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2953  GACACGTCCGTC        
Eco47III      729  AGCGCT               AGC^GCT
             2407  AGCGCT              
Eco81I        268  CCTCAGG              CC^T[ACGT]AGG
              606  CCTGAGG             
HgaI         1569  GACGC                GACGC(5/10)
             2292  GACGC               
Hin1I        1119  GGCGCC               G[GA]^CG[CT]C
             2292  GACGCC              
HindIII       441  AAGCTT               A^AGCTT
             3173  AAGCTT              
MboI         1242  GATC                 ^GATC
             3156  GATC                
NcoI           92  CCATGG               C^CATGG
             1194  CCATGG              
NdeII        1242  GATC                 ^GATC
             3156  GATC                
PflMI        3063  CCAGGAGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3288  CCACAGCGTGG         
PpuMI           7  GGGTCCT              [GA]G^G[AT]CC[CT]
              610  AGGTCCC             
SapI         2811  GAAGAGC              GCTCTTC(1/4)
             3193  GAAGAGC             
Sau3AI       1242  GATC                 ^GATC
             3156  GATC                
Tsp509I      2207  AATT                 ^AATT
             2274  AATT                
Van91I       3063  CCAGGAGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3288  CCACAGCGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwNI       
ApaI        AspHI       AvaI        AvaII       BanI        
BanII       BbvI        BcnI        BfaI        BlpI        
BmyI        BpmI        Bpu1102I    BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BssSI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DraII       DraIII      DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboII       
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NciI        NgoMIV      NlaIII      
NlaIV       NspI        PleI        PstI        RsaI        
SacI        Sau96I      ScrFI       SfaNI       SfcI        
SmaI        StyI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     XmaCI       XmaI        
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