Restriction map for pF1KA0182
 (Linear) (Six-base) MAPSORT of: KIAA0182.seq  Check: 8966  from: 1  to: 3432



KIAA0182.orf

 With 182 enzymes: SgfI * 

                        July 23, 2007 10:03  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     314     683    1332    3204    3432 
   Size:        314     369     649    1872     228

  Fragments arranged by size: 

                1872     649     369     314     228
 
AccI GT'mk_AC

Cuts at:      0    2317    3234    3432 
   Size:       2317     917     198

  Fragments arranged by size: 

                2317     917     198
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     558    1120    1918    3432 
   Size:        558     562     798    1514

  Fragments arranged by size: 

                1514     798     562     558
 
AfeI AGC'GCT

Cuts at:      0     731    2409    3432 
   Size:        731    1678    1023

  Fragments arranged by size: 

                1678    1023     731
 
AflIII A'CryG_T

Cuts at:      0    2141    2954    3432 
   Size:       2141     813     478

  Fragments arranged by size: 

                2141     813     478
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1314    3432 
   Size:       1314    2118
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1528    1560    3432 
   Size:       1528      32    1872

  Fragments arranged by size: 

                1872    1528      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1347    1359    2658    3432 
   Size:       1347      12    1299     774

  Fragments arranged by size: 

                1347    1299     774      12
 
ApaI G_GGCC'C

Cuts at:      0     100     960    1793    1799    1938    2100    3055    3432 
   Size:        100     860     833       6     139     162     955     377

  Fragments arranged by size: 

                 955     860     833     377     162     139     100       6
            
 
ApaLI G'TGCA_C

Cuts at:      0     394    3432 
   Size:        394    3038
 
AvaI C'yCGr_G

Cuts at:      0     703     959    1085    1786    1792    1813    3432 
   Size:        703     256     126     701       6      21    1619

  Fragments arranged by size: 

                1619     703     701     256     126      21       6
 
AvrII C'CTAG_G

Cuts at:      0    3403    3432 
   Size:       3403      29
 
BanI G'GyrC_C

Cuts at:      0     405    1119    1152    1173    1380    3432 
   Size:        405     714      33      21     207    2052

  Fragments arranged by size: 

                2052     714     405     207      33      21
 
BanII G_rGCy'C

Cuts at:      0     100     260     710     960     998    1622    1793    1799 
   Size:        100     160     450     250      38     624     171       6

Cuts at:   1799    1814    1886    1938    2100    2831    3035    3055    3432 
   Size:         15      72      52     162     731     204      20     377

  Fragments arranged by size: 

                 731     624     450     377     250     204     171     162
                 160     100      72      52      38      20      15       6
            
 
BbeI G_GCGC'C

Cuts at:      0    1123    3432 
   Size:       1123    2309
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1656    3432 
   Size:       1656    1776
 
BbvCI CC'TCA_GC

Cuts at:      0     757    3262    3432 
   Size:        757    2505     170

  Fragments arranged by size: 

                2505     757     170
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     657     691    3432 
   Size:        657      34    2741

  Fragments arranged by size: 

                2741     657      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     985    1917    3432 
   Size:        985     932    1515

  Fragments arranged by size: 

                1515     985     932
 
BlpI GC'TnA_GC

Cuts at:      0     310    1808    1885    2707    3432 
   Size:        310    1498      77     822     725

  Fragments arranged by size: 

                1498     822     725     310      77
 
Bme1580I G_kGCm'C

Cuts at:      0      92     100     134     398     410     960    1157    1178 
   Size:         92       8      34     264      12     550     197      21

Cuts at:   1178    1793    1799    1938    2100    3055    3432 
   Size:        615       6     139     162     955     377

  Fragments arranged by size: 

                 955     615     550     377     264     197     162     139
                  92      34      21      12       8       6
 
BmgBI CAC'GTC

Cuts at:      0    2144    2957    3432 
   Size:       2144     813     475

  Fragments arranged by size: 

                2144     813     475
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     407    1918    3432 
   Size:        407    1511    1514

  Fragments arranged by size: 

                1514    1511     407
 
BmtI G_CTAG'C

Cuts at:      0    2680    3432 
   Size:       2680     752
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3300    3432 
   Size:       3300     132
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     207     367     955    1012    1369    1786    2026 
   Size:        201       6     160     588      57     357     417     240

Cuts at:   2026    2741    2976    3226    3262    3331    3432 
   Size:        715     235     250      36      69     101

  Fragments arranged by size: 

                 715     588     417     357     250     240     235     201
                 160     101      69      57      36       6
 
Bpu10I CC'TnA_GC

Cuts at:      0     757     790    2978    3262    3432 
   Size:        757      33    2188     284     170

  Fragments arranged by size: 

                2188     757     284     170      33
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3333    3432 
   Size:       3333      99
 
BsaAI yAC'GTr

Cuts at:      0     402    3432 
   Size:        402    3030
 
BsaHI Gr'CG_yC

Cuts at:      0    1120    2293    3432 
   Size:       1120    1173    1139

  Fragments arranged by size: 

                1173    1139    1120
 
BsaWI w'CCGG_w

Cuts at:      0    1149    3432 
   Size:       1149    2283
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      46      76     144     174     213     243     365     395 
   Size:         46      30      68      30      39      30     122      30

Cuts at:    395     733     763    1252    1282    2026    2056    2490    2520 
   Size:        338      30     489      30     744      30     434      30

Cuts at:   2520    3432 
   Size:        912

  Fragments arranged by size: 

                 912     744     489     434     338     122      68      46
                  39      30      30      30      30      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     204     799    1063    1273    1276    1699    2023    2098 
   Size:        204     595     264     210       3     423     324      75

Cuts at:   2098    2101    3100    3103    3112    3139    3321    3432 
   Size:          3     999       3       9      27     182     111

  Fragments arranged by size: 

                 999     595     423     324     264     210     204     182
                 111      75      27       9       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     133     340     535     997    1177    1505    1621    3011 
   Size:        133     207     195     462     180     328     116    1390

Cuts at:   3011    3432 
   Size:        421

  Fragments arranged by size: 

                1390     462     421     328     207     195     180     133
                 116
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     640     661     718    1502    1579    2750    3432 
   Size:        373     267      21      57     784      77    1171     682

  Fragments arranged by size: 

                1171     784     682     373     267      77      57      21
            
 
BsiHKAI G_wGCw'C

Cuts at:      0     398     710    1400    1414    1772    1814    1886    3432 
   Size:        398     312     690      14     358      42      72    1546

  Fragments arranged by size: 

                1546     690     398     358     312      72      42      14
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1561    2532    3432 
   Size:       1561     971     900

  Fragments arranged by size: 

                1561     971     900
 
Bsp1286I G_dGCh'C

Cuts at:      0      92     100     134     260     398     410     710     960 
   Size:         92       8      34     126     138      12     300     250

Cuts at:    960     998    1157    1178    1400    1414    1622    1772    1793 
   Size:         38     159      21     222      14     208     150      21

Cuts at:   1793    1799    1814    1886    1938    2100    2831    3035    3055 
   Size:          6      15      72      52     162     731     204      20

Cuts at:   3055    3432 
   Size:        377

  Fragments arranged by size: 

                 731     377     300     250     222     208     204     162
                 159     150     138     126      92      72      52      38
                  34      21      21      20      15      14      12       8
                   6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     314     683    1332    3204    3432 
   Size:        314     369     649    1872     228

  Fragments arranged by size: 

                1872     649     369     314     228
 
BsrBI CCG'CTC

Cuts at:      0     264     849    1053    1659    3210    3342    3432 
   Size:        264     585     204     606    1551     132      90

  Fragments arranged by size: 

                1551     606     585     264     204     132      90
 
BsrDI GCAATG_nn'

Cuts at:      0    3398    3432 
   Size:       3398      34
 
BsrFI r'CCGG_y

Cuts at:      0     200     211    1310    1370    1383    1916    2577    3432 
   Size:        200      11    1099      60      13     533     661     855

  Fragments arranged by size: 

                1099     855     661     533     200      60      13      11
            
 
BssSI C'ACGA_G

Cuts at:      0    1438    1748    2863    3432 
   Size:       1438     310    1115     569

  Fragments arranged by size: 

                1438    1115     569     310
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1029    1152    1344    3432 
   Size:       1029     123     192    2088

  Fragments arranged by size: 

                2088    1029     192     123
 
BstBI TT'CG_AA

Cuts at:      0    2360    3432 
   Size:       2360    1072
 
BstEII G'GTnAC_C

Cuts at:      0     150    3432 
   Size:        150    3282
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     543     990    3019    3432 
   Size:        543     447    2029     413

  Fragments arranged by size: 

                2029     543     447     413
 
Bsu36I CC'TnA_GG

Cuts at:      0     269     607    3432 
   Size:        269     338    2825

  Fragments arranged by size: 

                2825     338     269
 
BtgI C'CryG_G

Cuts at:      0      92     146    1010    1194    1523    1693    1930    2618 
   Size:         92      54     864     184     329     170     237     688

Cuts at:   2618    2821    3432 
   Size:        203     611

  Fragments arranged by size: 

                 864     688     611     329     237     203     184     170
                  92      54
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     141     682    2093    3432 
   Size:        141     541    1411    1339

  Fragments arranged by size: 

                1411    1339     541     141
 
BtsI GCAGTG_nn'

Cuts at:      0     234     484     512     652    1227    2489    3038    3432 
   Size:        234     250      28     140     575    1262     549     394

  Fragments arranged by size: 

                1262     575     549     394     250     234     140      28
            
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2089    2124    3432 
   Size:       2089      35    1308

  Fragments arranged by size: 

                2089    1308      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     129     402     405    1173    3294    3432 
   Size:        129     273       3     768    2121     138

  Fragments arranged by size: 

                2121     768     273     138     129       3
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1433    2959    3432 
   Size:       1433    1526     473

  Fragments arranged by size: 

                1526    1433     473
 
EaeI y'GGCC_r

Cuts at:      0      47     509     977    1013    1035    1449    1868    2114 
   Size:         47     462     468      36      22     414     419     246

Cuts at:   2114    2486    3432 
   Size:        372     946

  Fragments arranged by size: 

                 946     468     462     419     414     372     246      47
                  36      22
 
EarI CTCTTCn'nnn_

Cuts at:      0    2410    2806    2837    3089    3188    3432 
   Size:       2410     396      31     252      99     244

  Fragments arranged by size: 

                2410     396     252     244      99      31
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1402    1727    2250    3313    3432 
   Size:       1402     325     523    1063     119

  Fragments arranged by size: 

                1402    1063     523     325     119
 
EcoICRI GAG'CTC

Cuts at:      0     708    1812    1884    3432 
   Size:        708    1104      72    1548

  Fragments arranged by size: 

                1548    1104     708      72
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     207     367     558     955    1012    1120    1369 
   Size:        201       6     160     191     397      57     108     249

Cuts at:   1369    1786    1918    2026    2741    2976    3226    3262    3331 
   Size:        417     132     108     715     235     250      36      69

Cuts at:   3331    3432 
   Size:        101

  Fragments arranged by size: 

                 715     417     397     250     249     235     201     191
                 160     132     108     108     101      69      57      36
                   6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1861    3432 
   Size:       1861    1571
 
EcoO109I rG'GnC_Cy

Cuts at:      0       8      97     611     987    1796    1935    2096    2715 
   Size:          8      89     514     376     809     139     161     619

Cuts at:   2715    2892    3051    3052    3432 
   Size:        177     159       1     380

  Fragments arranged by size: 

                 809     619     514     380     376     177     161     159
                 139      89       8       1
 
EcoRV GAT'ATC

Cuts at:      0    3424    3432 
   Size:       3424       8
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2452    3432 
   Size:       2452     980
 
FseI GG_CCGG'CC

Cuts at:      0    1374    3432 
   Size:       1374    2058
 
HaeII r_GCGC'y

Cuts at:      0     733     942    1123    1980    2314    2411    2765    3432 
   Size:        733     209     181     857     334      97     354     667

  Fragments arranged by size: 

                 857     733     667     354     334     209     181      97
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     144     176     199     231     278     310     733     765 
   Size:        144      32      23      32      47      32     423      32

Cuts at:    765    1300    1332    2131    2163    2490    2502    2522    2534 
   Size:        535      32     799      32     327      12      20      12

Cuts at:   2534    2971    3003    3064    3096    3432 
   Size:        437      32      61      32     336

  Fragments arranged by size: 

                 799     535     437     423     336     327     144      61
                  47      32      32      32      32      32      32      32
                  32      23      20      12      12
 
HincII GTy'rAC

Cuts at:      0    2952    3432 
   Size:       2952     480
 
HindIII A'AGCT_T

Cuts at:      0     441    3173    3432 
   Size:        441    2732     259

  Fragments arranged by size: 

                2732     441     259
 
KasI G'GCGC_C

Cuts at:      0    1119    3432 
   Size:       1119    2313
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     187    1158    1214    3050    3432 
   Size:        187     971      56    1836     382

  Fragments arranged by size: 

                1836     971     382     187      56
 
MscI TGG'CCA

Cuts at:      0    1037    3432 
   Size:       1037    2395
 
MslI CAynn'nnrTG

Cuts at:      0    2782    3432 
   Size:       2782     650
 
MspA1I CmG'CkG

Cuts at:      0     387     945     954    1074    1284    1644    1848    1889 
   Size:        387     558       9     120     210     360     204      41

Cuts at:   1889    2620    2690    2790    3432 
   Size:        731      70     100     642

  Fragments arranged by size: 

                 731     642     558     387     360     210     204     120
                 100      70      41       9
 
NaeI GCC'GGC

Cuts at:      0     202     213    1372    1385    3432 
   Size:        202      11    1159      13    2047

  Fragments arranged by size: 

                2047    1159     202      13      11
 
NarI GG'CG_CC

Cuts at:      0    1120    3432 
   Size:       1120    2312
 
NcoI C'CATG_G

Cuts at:      0      92    1194    3432 
   Size:         92    1102    2238

  Fragments arranged by size: 

                2238    1102      92
 
NgoMIV G'CCGG_C

Cuts at:      0     200     211    1370    1383    3432 
   Size:        200      11    1159      13    2049

  Fragments arranged by size: 

                2049    1159     200      13      11
 
NheI G'CTAG_C

Cuts at:      0    2676    3432 
   Size:       2676     756
 
NspI r_CATG'y

Cuts at:      0     480     750    2745    3432 
   Size:        480     270    1995     687

  Fragments arranged by size: 

                1995     687     480     270
 
PasI CC'CwG_GG

Cuts at:      0     115    3432 
   Size:        115    3317
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3069    3294    3432 
   Size:       3069     225     138

  Fragments arranged by size: 

                3069     225     138
 
PfoI T'CCnGG_A

Cuts at:      0     217    2669    3432 
   Size:        217    2452     763

  Fragments arranged by size: 

                2452     763     217
 
PmlI CAC'GTG

Cuts at:      0     402    3432 
   Size:        402    3030
 
PpuMI rG'GwC_Cy

Cuts at:      0       8     611    3432 
   Size:          8     603    2821

  Fragments arranged by size: 

                2821     603       8
 
PspOMI G'GGCC_C

Cuts at:      0      96     956    1789    1795    1934    2096    3051    3432 
   Size:         96     860     833       6     139     162     955     381

  Fragments arranged by size: 

                 955     860     833     381     162     139      96       6
            
 
PstI C_TGCA'G

Cuts at:      0     522     776    1118    1973    2879    2900    2934    2987 
   Size:        522     254     342     855     906      21      34      53

Cuts at:   2987    3432 
   Size:        445

  Fragments arranged by size: 

                 906     855     522     445     342     254      53      34
                  21
 
PvuII CAG'CTG

Cuts at:      0    1074    3432 
   Size:       1074    2358
 
SacI G_AGCT'C

Cuts at:      0     710    1814    1886    3432 
   Size:        710    1104      72    1546

  Fragments arranged by size: 

                1546    1104     710      72
 
SacII CC_GC'GG

Cuts at:      0    2621    3432 
   Size:       2621     811
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2806    3188    3432 
   Size:       2806     382     244

  Fragments arranged by size: 

                2806     382     244
 
SbfI CC_TGCA'GG

Cuts at:      0    1118    3432 
   Size:       1118    2314
 
SfcI C'TryA_G

Cuts at:      0     518     772    1114    1969    2325    2875    2896    2930 
   Size:        518     254     342     855     356     550      21      34

Cuts at:   2930    2983    3255    3432 
   Size:         53     272     177

  Fragments arranged by size: 

                 855     550     518     356     342     272     254     177
                  53      34      21
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     985    3432 
   Size:        985    2447
 
SfoI GGC'GCC

Cuts at:      0    1121    3432 
   Size:       1121    2311
 
SmaI CCC'GGG

Cuts at:      0     961    1788    1794    3432 
   Size:        961     827       6    1638

  Fragments arranged by size: 

                1638     961     827       6
 
SmlI C'TyrA_G

Cuts at:      0    1813    3432 
   Size:       1813    1619
 
SphI G_CATG'C

Cuts at:      0    2745    3432 
   Size:       2745     687
 
SrfI GCCC'GGGC

Cuts at:      0    1788    1794    3432 
   Size:       1788       6    1638

  Fragments arranged by size: 

                1788    1638       6
 
StyI C'CwwG_G

Cuts at:      0      92     983    1194    1562    2771    3403    3432 
   Size:         92     891     211     368    1209     632      29

  Fragments arranged by size: 

                1209     891     632     368     211      92      29
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      93     439     674    1536    1840    2203    2784    2899 
   Size:         93     346     235     862     304     363     581     115

Cuts at:   2899    3432 
   Size:        533

  Fragments arranged by size: 

                 862     581     533     363     346     304     235     115
                  93
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     213     245     365     397     542     574    1252    1284 
   Size:        213      32     120      32     145      32     678      32

Cuts at:   1284    2843    2875    3432 
   Size:       1559      32     557

  Fragments arranged by size: 

                1559     678     557     213     145     120      32      32
                  32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2803    3432 
   Size:       2803     629
 
XhoI C'TCGA_G

Cuts at:      0    1813    3432 
   Size:       1813    1619
 
XmaI C'CCGG_G

Cuts at:      0     959    1786    1792    3432 
   Size:        959     827       6    1640

  Fragments arranged by size: 

                1640     959     827       6
 
XmnI GAAnn'nnTTC

Cuts at:      0    2374    3432 
   Size:       2374    1058
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AhdI     AloI    AlwNI
     ApaI    ApaLI     AvaI    AvrII     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstXI   Bsu36I
     BtgI    BtgZI     BtsI    CspCI   DraIII     DrdI     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI     FseI
    HaeII    Hin4I   HincII  HindIII     KasI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NspI     PasI
    PflMI     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII     SacI
    SacII     SapI     SbfI     SfcI     SfiI     SfoI     SmaI     SmlI
     SphI     SrfI     StyI    TaqII     TstI     XcmI     XhoI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AleI     AlfI     ApoI
     AscI     AseI    AsiSI     BaeI    BamHI    BciVI     BclI    BfrBI
    BglII    BpuEI    BsaBI    BsiEI    BsiWI     BsmI    BspEI    BspHI
    BsrGI   BssHII    BstYI  BstZ17I     ClaI     DraI     EagI    EcoRI
     FspI    FspAI     HpaI     KpnI     MfeI     MluI     NdeI     NotI
     NruI     NsiI     PacI     PciI     PmeI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SalI    SanDI     ScaI    SexAI
     SgfI    SgrAI    SnaBI     SpeI     SspI     StuI     SwaI     TatI
  Tth111I     XbaI     ZraI
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