Restriction map for pF1KSDF0201
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# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AhdI        AlwI        Aor51HI     AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AviII       AvrII       BamHI       BbeI        BbrPI       
BbvCI       BciVI       BclI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaI        BseAI       BsiEI       BsiWI       BsmBI       
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstYI       BstZ17I     CelII       CfoI        
ClaI        CpoI        DraI        DraIII      EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HhaI        HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
Ksp632I     MamI        MfeI        MflI        MluI        
MroI        MseI        MunI        NarI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SalI        SapI        
SbfI        ScaI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspI        SwaI        
Tru9I       XbaI        XhoI        XhoII       XmnI        


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# ONE CUT ENZYMES (   45 enzymes) #
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AccII          71  CGCG                 CG^CG
AcsI         1777  AAATTT               [GA]^AATT[CT]
AflIII       1895  ACGTGT               A^C[GA][CT]GT
Alw44I       1876  GTGCAC               G^TGCAC
ApaLI        1876  GTGCAC               G^TGCAC
ApoI         1777  AAATTT               [GA]^AATT[CT]
AspI         2011  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1800  TGGCCA               TGG^CCA
BbsI           24  GTCTTC               GAAGAC(2/6)
BcgI         1701  CGAGCTGTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI         1193  CTAG                 C^TAG
BpuAI          24  GTCTTC               GAAGAC(2/6)
BsaWI        1784  ACCGGA               [AT]^CCGG[AT]
BseRI        2076  CTCCTC               GAGGAG(10/8)
BsgI         2023  GTGCAG               GTGCAG(16/14)
BsmI         1172  GCATTC               GAATGC(1/-1)
Bsp1407I     1910  TGTACA               T^GTACA
BsrGI        1910  TGTACA               T^GTACA
BstEII       1180  GGTGACC              G^GT[ACGT]ACC
BstPI        1180  GGTGACC              G^GT[ACGT]ACC
BstUI          71  CGCG                 CG^CG
BstXI        1971  CCAGGCATCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII         601  GATC                 ^GATC
EcoNI        1965  CCTGGACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1180  GGTGACC              G^GT[ACGT]ACC
KspI           70  CCGCGG               CCGC^GG
MaeI         1193  CTAG                 C^TAG
MboI          601  GATC                 ^GATC
MluNI        1800  TGGCCA               TGG^CCA
MscI         1800  TGGCCA               TGG^CCA
MvnI           71  CGCG                 CG^CG
NaeI         1926  GCCGGC               GCC^GGC
NdeII         601  GATC                 ^GATC
NgoMIV       1926  GCCGGC               G^CCGGC
NspI         1766  GCATGC               [GA]CATG^[CT]
PflFI        2011  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI         1094  CTGCAG               CTGCA^G
SacII          70  CCGCGG               CCGC^GG
Sau3AI        601  GATC                 ^GATC
SfcI         1094  CTGCAG               C^T[GA][CT]AG
SmlI          610  CTCAAG               C^T[CT][GA]AG
SphI         1766  GCATGC               GCATG^C
SspBI        1910  TGTACA               T^GTACA
Tsp509I      1778  AATT                 ^AATT
Tth111I      2011  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AccI          829  GTAGAC               GT^[AC][GT]AC
             1855  GTCTAC              
AcyI           75  GGCGTC               G[GA]^CG[CT]C
             1852  GGCGTC              
BsaHI          75  GGCGTC               G[GA]^CG[CT]C
             1852  GGCGTC              
BsmAI         746  GAGAC                GTCTC(1/5)
             1612  GAGAC               
BspMI        1634  ACCTGC               ACCTGC(4/8)
             1837  ACCTGC              
DrdI           15  GACACCCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1397  GACTCCAGGGTC        
EaeI         1800  TGGCCA               [CT]^GGCC[GA]
             1928  CGGCCA              
HgaI           76  GCGTC                GACGC(5/10)
             1853  GCGTC               
Hin1I          75  GGCGTC               G[GA]^CG[CT]C
             1852  GGCGTC              
MaeIII       1181  GTGAC                ^GT[ACGT]AC
             1343  GTGAC               
MboII          25  TCTTC                GAAGA(8/7)
             1832  TCTTC               
NcoI          321  CCATGG               C^CATGG
             1248  CCATGG              
Tsp45I       1181  GTGAC                ^GT[GC]AC
             1343  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AocI        
ApaI        AspHI       AvaI        AvaII       BanI        
BanII       BbvI        BcnI        BglI        BmyI        
BpmI        BsaJI       BsiHKAI     BsiYI       BslI        
BsmFI       BsoBI       Bsp1286I    BsrFI       BsrI        
BssKI       BstF5I      BstNI       Bsu36I      Cac8I       
Cfr10I      Cfr13I      DdeI        DraII       DsaI        
Eco81I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        MaeII       MnlI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NlaIII      NlaIV       PflMI       PleI        
PpuMI       PvuII       RsaI        Sau96I      ScrFI       
SexAI       SfaNI       SfiI        SmaI        StuI        
StyI        TaqI        TfiI        TseI        TspRI       
TthHB8I     Van91I      XcmI        XmaCI       XmaI        
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