(Circular) (Six-base) MAPSORT of: pF1KSDF0136.seq Check: 3237 from: 1 to: 5554 pF1KSDF0136 5554 bp With 182 enzymes: SgfI * June 20, 2008 10:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1195 2136 2173 1195 Size: 941 37 4576 Fragments arranged by size: 4576 941 37 AccI GT'mk_AC Cuts at: 2590 2590 Size: 5554 Acc65I G'GTAC_C Cuts at: 2273 2568 2273 Size: 295 5259 AclI AA'CG_TT Cuts at: 5394 5394 Size: 5554 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 847 1369 1882 3377 3809 4558 4826 847 Size: 522 513 1495 432 749 268 1575 Fragments arranged by size: 1575 1495 749 522 513 432 268 AflII C'TTAA_G Cuts at: 633 633 Size: 5554 AflIII A'CryG_T Cuts at: 2904 4010 4987 2904 Size: 1106 977 3471 Fragments arranged by size: 3471 1106 977 AgeI A'CCGG_T Cuts at: 1775 2270 3942 1775 Size: 495 1672 3387 Fragments arranged by size: 3387 1672 495 AleI CACnn'nnGTG Cuts at: 1198 1198 Size: 5554 AlwNI CAG_nnn'CTG Cuts at: 138 591 804 1519 1770 1929 2857 4426 138 Size: 453 213 715 251 159 928 1569 1266 Fragments arranged by size: 1569 1266 928 715 453 251 213 159 ApaI G_GGCC'C Cuts at: 190 943 976 1584 190 Size: 753 33 608 4160 Fragments arranged by size: 4160 753 608 33 ApaLI G'TGCA_C Cuts at: 214 255 1107 2266 4324 214 Size: 41 852 1159 2058 1444 Fragments arranged by size: 2058 1444 1159 852 41 ApoI r'AATT_y Cuts at: 2556 4907 5163 2556 Size: 2351 256 2947 Fragments arranged by size: 2947 2351 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5554 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5554 AvaI C'yCGr_G Cuts at: 334 475 678 2157 2572 334 Size: 141 203 1479 415 3316 Fragments arranged by size: 3316 1479 415 203 141 BamHI G'GATC_C Cuts at: 2577 2577 Size: 5554 BanI G'GyrC_C Cuts at: 340 409 2037 2113 2273 2505 2568 2982 3230 Size: 69 1628 76 160 232 63 414 248 Cuts at: 3230 3265 340 Size: 35 2629 Fragments arranged by size: 2629 1628 414 248 232 160 76 69 63 35 BanII G_rGCy'C Cuts at: 190 584 943 976 1004 1584 2566 3596 190 Size: 394 359 33 28 580 982 1030 2148 Fragments arranged by size: 2148 1030 982 580 394 359 33 28 BbeI G_GCGC'C Cuts at: 2041 2117 2986 3234 2041 Size: 76 869 248 4361 Fragments arranged by size: 4361 869 248 76 BbsI GAAGACnn'nnnn_ Cuts at: 1266 1479 1989 2017 2070 2115 2292 1266 Size: 213 510 28 53 45 177 4528 Fragments arranged by size: 4528 510 213 177 53 45 28 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 2482 2516 2482 Size: 34 5520 BciVI GTATCCnnnnn_n' Cuts at: 3450 4213 5554 3450 Size: 763 1341 3450 Fragments arranged by size: 3450 1341 763 BclI T'GATC_A Cuts at: 3072 3072 Size: 5554 BglI GCCn_nnn'nGGC Cuts at: 704 799 848 2046 704 Size: 95 49 1198 4212 Fragments arranged by size: 4212 1198 95 49 BglII A'GATC_T Cuts at: 3067 5106 3067 Size: 2039 3515 BlpI GC'TnA_GC Cuts at: 2664 2664 Size: 5554 Bme1580I G_kGCm'C Cuts at: 190 218 259 343 943 976 1111 1584 2270 Size: 28 41 84 600 33 135 473 686 Cuts at: 2270 3177 3270 4328 190 Size: 907 93 1058 1416 Fragments arranged by size: 1416 1058 907 686 600 473 135 93 84 41 33 28 BmgBI CAC'GTC Cuts at: 1485 1485 Size: 5554 BmrI ACTGGGnnnn_n' Cuts at: 417 2764 2845 3180 5326 417 Size: 2347 81 335 2146 645 Fragments arranged by size: 2347 2146 645 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3919 3919 Size: 5554 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 295 544 565 568 837 1018 1306 1633 1657 Size: 249 21 3 269 181 288 327 24 Cuts at: 1657 1927 2248 295 Size: 270 321 3601 Fragments arranged by size: 3601 327 321 288 270 269 249 181 24 21 3 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 865 1657 2368 2728 3607 4101 4399 865 Size: 792 711 360 879 494 298 2020 Fragments arranged by size: 2020 879 792 711 494 360 298 BsaI GGTCTCn'nnnn_ Cuts at: 157 2253 5258 157 Size: 2096 3005 453 Fragments arranged by size: 3005 2096 453 BsaAI yAC'GTr Cuts at: 1647 2544 2905 1647 Size: 897 361 4296 Fragments arranged by size: 4296 897 361 BsaBI GATnn'nnATC Cuts at: 6 3066 5105 6 Size: 3060 2039 455 Fragments arranged by size: 3060 2039 455 BsaHI Gr'CG_yC Cuts at: 1727 2038 2114 2983 3231 3935 5431 1727 Size: 311 76 869 248 704 1496 1850 Fragments arranged by size: 1850 1496 869 704 311 248 76 BsaWI w'CCGG_w Cuts at: 1775 2177 2270 2741 3262 3942 4216 4363 4774 Size: 402 93 471 521 680 274 147 411 Cuts at: 4774 1775 Size: 2555 Fragments arranged by size: 2555 680 521 471 411 402 274 147 93 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 562 565 568 994 1558 1881 1951 2482 2487 Size: 3 3 426 564 323 70 531 5 Cuts at: 2487 2509 562 Size: 22 3607 Fragments arranged by size: 3607 564 531 426 323 70 22 5 3 3 BseYI C'CCAG_C Cuts at: 131 190 382 502 1425 1461 1710 1745 1938 Size: 59 192 120 923 36 249 35 193 Cuts at: 1938 2977 3735 4314 4810 131 Size: 1039 758 579 496 875 Fragments arranged by size: 1039 923 875 758 579 496 249 193 192 120 59 36 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 463 474 972 1993 463 Size: 11 498 1021 4024 Fragments arranged by size: 4024 1021 498 11 BsiEI CG_ry'CG Cuts at: 104 3094 3140 4350 104 Size: 2990 46 1210 1308 Fragments arranged by size: 2990 1308 1210 46 BsiHKAI G_wGCw'C Cuts at: 218 259 352 584 863 1004 1111 1949 2074 Size: 41 93 232 279 141 107 838 125 Cuts at: 2074 2164 2270 2566 3344 3534 4328 218 Size: 90 106 296 778 190 794 1444 Fragments arranged by size: 1444 838 794 778 296 279 232 190 141 125 107 106 93 90 41 Bsp1286I G_dGCh'C Cuts at: 190 218 259 343 352 584 863 943 976 Size: 28 41 84 9 232 279 80 33 Cuts at: 976 1004 1111 1584 1949 2074 2164 2270 2566 Size: 28 107 473 365 125 90 106 296 Cuts at: 2566 3177 3270 3344 3534 3596 4328 190 Size: 611 93 74 190 62 732 1416 Fragments arranged by size: 1416 732 611 473 365 296 279 232 190 125 107 106 93 90 84 80 74 62 41 33 28 28 9 BspEI T'CCGG_A Cuts at: 2177 4774 2177 Size: 2597 2957 BspHI T'CATG_A Cuts at: 1499 4730 1499 Size: 3231 2323 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1195 2136 2173 2602 2816 3118 3499 1195 Size: 941 37 429 214 302 381 3250 Fragments arranged by size: 3250 941 429 381 302 214 37 BsrBI CCG'CTC Cuts at: 50 294 491 3844 3898 5384 5549 50 Size: 244 197 3353 54 1486 165 55 Fragments arranged by size: 3353 1486 244 197 165 55 54 BsrDI GCAATG_nn' Cuts at: 1889 2406 3464 1889 Size: 517 1058 3979 Fragments arranged by size: 3979 1058 517 BsrFI r'CCGG_y Cuts at: 464 734 1775 2045 2203 2270 3550 3731 3942 Size: 270 1041 270 158 67 1280 181 211 Cuts at: 3942 464 Size: 2076 Fragments arranged by size: 2076 1280 1041 270 270 211 181 158 67 BsrGI T'GTAC_A Cuts at: 1202 1202 Size: 5554 BssSI C'ACGA_G Cuts at: 3823 4183 3823 Size: 360 5194 BstAPI GCAn_nnn'nTGC Cuts at: 1106 2512 2929 1106 Size: 1406 417 3731 Fragments arranged by size: 3731 1406 417 BstBI TT'CG_AA Cuts at: 110 110 Size: 5554 BstXI CCAn_nnnn'nTGG Cuts at: 543 1081 2022 543 Size: 538 941 4075 Fragments arranged by size: 4075 941 538 BstYI r'GATC_y Cuts at: 1 2577 3044 3067 3402 3648 4662 4748 4760 Size: 2576 467 23 335 246 1014 86 12 Cuts at: 4760 5106 1 Size: 346 449 Fragments arranged by size: 2576 1014 467 449 346 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 944 944 Size: 5554 BtgI C'CryG_G Cuts at: 125 536 811 1472 125 Size: 411 275 661 4207 Fragments arranged by size: 4207 661 411 275 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3502 3683 3846 3502 Size: 181 163 5210 Fragments arranged by size: 5210 181 163 BtsI GCAGTG_nn' Cuts at: 602 2795 602 Size: 2193 3361 DraI TTT'AAA Cuts at: 2551 2551 Size: 5554 DrdI GACnn_nn'nnGTC Cuts at: 3258 4118 3258 Size: 860 4694 EaeI y'GGCC_r Cuts at: 353 371 396 732 1047 1361 1475 1524 1604 Size: 18 25 336 315 314 114 49 80 Cuts at: 1604 1649 1679 2024 2324 3137 3311 3702 3729 Size: 45 30 345 300 813 174 391 27 Cuts at: 3729 5076 353 Size: 1347 831 Fragments arranged by size: 1347 831 813 391 345 336 315 314 300 174 114 80 49 45 30 27 25 18 EagI C'GGCC_G Cuts at: 3137 3137 Size: 5554 EarI CTCTTCn'nnn_ Cuts at: 661 1858 1954 3575 3785 661 Size: 1197 96 1621 210 2430 Fragments arranged by size: 2430 1621 1197 210 96 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 483 499 780 1887 3760 4072 4218 5364 483 Size: 16 281 1107 1873 312 146 1146 673 Fragments arranged by size: 1873 1146 1107 673 312 281 146 16 EcoICRI GAG'CTC Cuts at: 582 1002 2564 582 Size: 420 1562 3572 Fragments arranged by size: 3572 1562 420 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 295 544 565 568 837 847 1018 1306 1369 Size: 249 21 3 269 10 171 288 63 Cuts at: 1369 1633 1657 1882 1927 2248 3377 3809 4558 Size: 264 24 225 45 321 1129 432 749 Cuts at: 4558 4826 295 Size: 268 1023 Fragments arranged by size: 1129 1023 749 432 321 288 269 268 264 249 225 171 63 45 24 21 10 3 EcoNI CCTnn'n_nnAGG Cuts at: 547 574 1324 2383 547 Size: 27 750 1059 3718 Fragments arranged by size: 3718 1059 750 27 EcoO109I rG'GnC_Cy Cuts at: 186 187 674 801 940 973 1580 1581 2239 Size: 1 487 127 139 33 607 1 658 Cuts at: 2239 2691 186 Size: 452 3049 Fragments arranged by size: 3049 658 607 487 452 139 127 33 1 1 EcoRI G'AATT_C Cuts at: 2556 2556 Size: 5554 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3571 3571 Size: 5554 FspI TGC'GCA Cuts at: 3333 3333 Size: 5554 HaeII r_GCGC'y Cuts at: 784 819 2041 2117 2986 3234 4258 5237 784 Size: 35 1222 76 869 248 1024 979 1101 Fragments arranged by size: 1222 1101 1024 979 869 248 76 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 398 430 1494 1526 3394 3424 3426 3456 398 Size: 32 1064 32 1868 30 2 30 2496 Fragments arranged by size: 2496 1868 1064 32 32 30 30 2 HincII GTy'rAC Cuts at: 2591 2591 Size: 5554 HindIII A'AGCT_T Cuts at: 2434 2434 Size: 5554 KasI G'GCGC_C Cuts at: 2037 2113 2982 3230 2037 Size: 76 869 248 4361 Fragments arranged by size: 4361 869 248 76 KpnI G_GTAC'C Cuts at: 2277 2572 2277 Size: 295 5259 MluI A'CGCG_T Cuts at: 4987 4987 Size: 5554 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 4225 4409 4225 Size: 184 5370 MscI TGG'CCA Cuts at: 373 1526 1651 1681 2026 3313 373 Size: 1153 125 30 345 1287 2614 Fragments arranged by size: 2614 1287 1153 345 125 30 MslI CAynn'nnrTG Cuts at: 1036 1198 2347 3541 3668 1036 Size: 162 1149 1194 127 2922 Fragments arranged by size: 2922 1194 1149 162 127 MspA1I CmG'CkG Cuts at: 606 710 1374 2119 2663 2977 3337 4352 4597 Size: 104 664 745 544 314 360 1015 245 Cuts at: 4597 606 Size: 1563 Fragments arranged by size: 1563 1015 745 664 544 360 314 245 104 NaeI GCC'GGC Cuts at: 466 736 2047 2205 3733 466 Size: 270 1311 158 1528 2287 Fragments arranged by size: 2287 1528 1311 270 158 NarI GG'CG_CC Cuts at: 2038 2114 2983 3231 2038 Size: 76 869 248 4361 Fragments arranged by size: 4361 869 248 76 NcoI C'CATG_G Cuts at: 125 125 Size: 5554 NgoMIV G'CCGG_C Cuts at: 464 734 2045 2203 3731 464 Size: 270 1311 158 1528 2287 Fragments arranged by size: 2287 1528 1311 270 158 NruI TCG'CGA Cuts at: 3915 3915 Size: 5554 NspI r_CATG'y Cuts at: 381 618 1143 2605 3105 4014 381 Size: 237 525 1462 500 909 1921 Fragments arranged by size: 1921 1462 909 525 500 237 PasI CC'CwG_GG Cuts at: 169 1115 2243 2261 169 Size: 946 1128 18 3462 Fragments arranged by size: 3462 1128 946 18 PciI A'CATG_T Cuts at: 4010 4010 Size: 5554 PflMI CCAn_nnn'nTGG Cuts at: 138 591 1710 138 Size: 453 1119 3982 Fragments arranged by size: 3982 1119 453 PfoI T'CCnGG_A Cuts at: 239 1904 239 Size: 1665 3889 PmeI GTTT'AAAC Cuts at: 2551 2551 Size: 5554 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4693 4725 4693 Size: 32 5522 PpuMI rG'GwC_Cy Cuts at: 674 674 Size: 5554 PspOMI G'GGCC_C Cuts at: 186 939 972 1580 186 Size: 753 33 608 4160 Fragments arranged by size: 4160 753 608 33 PspXI vC'TCGA_Gb Cuts at: 2157 2157 Size: 5554 PstI C_TGCA'G Cuts at: 284 800 1660 1736 2599 3284 284 Size: 516 860 76 863 685 2554 Fragments arranged by size: 2554 863 860 685 516 76 PvuI CG_AT'CG Cuts at: 104 104 Size: 5554 PvuII CAG'CTG Cuts at: 1374 2977 3337 1374 Size: 1603 360 3591 Fragments arranged by size: 3591 1603 360 RsrII CG'GwC_CG Cuts at: 3747 3747 Size: 5554 SacI G_AGCT'C Cuts at: 584 1004 2566 584 Size: 420 1562 3572 Fragments arranged by size: 3572 1562 420 SalI G'TCGA_C Cuts at: 2589 2589 Size: 5554 SapI GCTCTTCn'nnn_ Cuts at: 661 1954 3575 3785 661 Size: 1293 1621 210 2430 Fragments arranged by size: 2430 1621 1293 210 SbfI CC_TGCA'GG Cuts at: 800 1736 2599 800 Size: 936 863 3755 Fragments arranged by size: 3755 936 863 ScaI AGT'ACT Cuts at: 427 5071 427 Size: 4644 910 SfcI C'TryA_G Cuts at: 33 280 796 1053 1656 1732 2595 3280 4275 Size: 247 516 257 603 76 863 685 995 Cuts at: 4275 4466 33 Size: 191 1121 Fragments arranged by size: 1121 995 863 685 603 516 257 247 191 76 SfoI GGC'GCC Cuts at: 2039 2115 2984 3232 2039 Size: 76 869 248 4361 Fragments arranged by size: 4361 869 248 76 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5554 SmaI CCC'GGG Cuts at: 2574 2574 Size: 5554 SmlI C'TyrA_G Cuts at: 633 880 1672 2157 2383 2707 3622 4116 4378 Size: 247 792 485 226 324 915 494 262 Cuts at: 4378 633 Size: 1809 Fragments arranged by size: 1809 915 792 494 485 324 262 247 226 SnaBI TAC'GTA Cuts at: 2544 2544 Size: 5554 SpeI A'CTAG_T Cuts at: 67 67 Size: 5554 SphI G_CATG'C Cuts at: 381 2605 3105 381 Size: 2224 500 2830 Fragments arranged by size: 2830 2224 500 SspI AAT'ATT Cuts at: 1335 1335 Size: 5554 StuI AGG'CCT Cuts at: 995 995 Size: 5554 StyI C'CwwG_G Cuts at: 125 2686 3040 125 Size: 2561 354 2639 Fragments arranged by size: 2639 2561 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 358 1042 1111 1128 2205 2253 3135 3268 3727 Size: 684 69 17 1077 48 882 133 459 Cuts at: 3727 3938 358 Size: 211 1974 Fragments arranged by size: 1974 1077 882 684 459 211 133 69 48 17 TatI w'GTAC_w Cuts at: 425 1040 1202 5069 425 Size: 615 162 3867 910 Fragments arranged by size: 3867 910 615 162 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 350 382 1819 1851 5071 5103 350 Size: 32 1437 32 3220 32 801 Fragments arranged by size: 3220 1437 801 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1795 3349 1795 Size: 1554 4000 XbaI T'CTAG_A Cuts at: 2583 2583 Size: 5554 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3047 3047 Size: 5554 XhoI C'TCGA_G Cuts at: 2157 2157 Size: 5554 XmaI C'CCGG_G Cuts at: 2572 2572 Size: 5554 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflII AflIII AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AatII AfeI AhdI AlfI AloI AscI AvrII BaeI BbvCI BfrBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NsiI PacI PmlI PshAI PsiI PsrI SacII SanDI SexAI SfiI SgrAI SrfI SwaI XmnI ZraI