Restriction map for pF1KSDF0136
 (Linear) (Six-base) MAPSORT of: FLJ00136.seq  Check: 956  from: 1  to: 2415



FLJ00136.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1069    2010    2047    2415 
   Size:       1069     941      37     368

  Fragments arranged by size: 

                1069     941     368      37
 
Acc65I G'GTAC_C

Cuts at:      0    2147    2415 
   Size:       2147     268
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     721    1243    1756    2415 
   Size:        721     522     513     659

  Fragments arranged by size: 

                 721     659     522     513
 
AflII C'TTAA_G

Cuts at:      0     507    2415 
   Size:        507    1908
 
AgeI A'CCGG_T

Cuts at:      0    1649    2144    2415 
   Size:       1649     495     271

  Fragments arranged by size: 

                1649     495     271
 
AleI CACnn'nnGTG

Cuts at:      0    1072    2415 
   Size:       1072    1343
 
AlwNI CAG_nnn'CTG

Cuts at:      0      12     465     678    1393    1644    1803    2415 
   Size:         12     453     213     715     251     159     612

  Fragments arranged by size: 

                 715     612     453     251     213     159      12
 
ApaI G_GGCC'C

Cuts at:      0      64     817     850    1458    2415 
   Size:         64     753      33     608     957

  Fragments arranged by size: 

                 957     753     608      64      33
 
ApaLI G'TGCA_C

Cuts at:      0      88     129     981    2140    2415 
   Size:         88      41     852    1159     275

  Fragments arranged by size: 

                1159     852     275      88      41
 
AvaI C'yCGr_G

Cuts at:      0     208     349     552    2031    2415 
   Size:        208     141     203    1479     384

  Fragments arranged by size: 

                1479     384     208     203     141
 
BanI G'GyrC_C

Cuts at:      0     214     283    1911    1987    2147    2379    2415 
   Size:        214      69    1628      76     160     232      36

  Fragments arranged by size: 

                1628     232     214     160      76      69      36
 
BanII G_rGCy'C

Cuts at:      0      64     458     817     850     878    1458    2415 
   Size:         64     394     359      33      28     580     957

  Fragments arranged by size: 

                 957     580     394     359      64      33      28
 
BbeI G_GCGC'C

Cuts at:      0    1915    1991    2415 
   Size:       1915      76     424

  Fragments arranged by size: 

                1915     424      76
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1140    1353    1863    1891    1944    1989    2166    2415 
   Size:       1140     213     510      28      53      45     177     249

  Fragments arranged by size: 

                1140     510     249     213     177      53      45      28
            
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2356    2390    2415 
   Size:       2356      34      25

  Fragments arranged by size: 

                2356      34      25
 
BglI GCCn_nnn'nGGC

Cuts at:      0     578     673     722    1920    2415 
   Size:        578      95      49    1198     495

  Fragments arranged by size: 

                1198     578     495      95      49
 
Bme1580I G_kGCm'C

Cuts at:      0      64      92     133     217     817     850     985    1458 
   Size:         64      28      41      84     600      33     135     473

Cuts at:   1458    2144    2415 
   Size:        686     271

  Fragments arranged by size: 

                 686     600     473     271     135      84      64      41
                  33      28
 
BmgBI CAC'GTC

Cuts at:      0    1359    2415 
   Size:       1359    1056
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     291    2415 
   Size:        291    2124
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     418     439     442     711     892    1180    1507 
   Size:        169     249      21       3     269     181     288     327

Cuts at:   1507    1531    1801    2122    2415 
   Size:         24     270     321     293

  Fragments arranged by size: 

                 327     321     293     288     270     269     249     181
                 169      24      21       3
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     739    1531    2242    2415 
   Size:        739     792     711     173

  Fragments arranged by size: 

                 792     739     711     173
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      31    2127    2415 
   Size:         31    2096     288

  Fragments arranged by size: 

                2096     288      31
 
BsaAI yAC'GTr

Cuts at:      0    1521    2415 
   Size:       1521     894
 
BsaHI Gr'CG_yC

Cuts at:      0    1601    1912    1988    2415 
   Size:       1601     311      76     427

  Fragments arranged by size: 

                1601     427     311      76
 
BsaWI w'CCGG_w

Cuts at:      0    1649    2051    2144    2415 
   Size:       1649     402      93     271

  Fragments arranged by size: 

                1649     402     271      93
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     436     439     442     868    1432    1755    1825    2356 
   Size:        436       3       3     426     564     323      70     531

Cuts at:   2356    2361    2383    2415 
   Size:          5      22      32

  Fragments arranged by size: 

                 564     531     436     426     323      70      32      22
                   5       3       3
 
BseYI C'CCAG_C

Cuts at:      0       5      64     256     376    1299    1335    1584    1619 
   Size:          5      59     192     120     923      36     249      35

Cuts at:   1619    1812    2415 
   Size:        193     603

  Fragments arranged by size: 

                 923     603     249     193     192     120      59      36
                  35       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     348     846    1867    2415 
   Size:        337      11     498    1021     548

  Fragments arranged by size: 

                1021     548     498     337      11
 
BsiHKAI G_wGCw'C

Cuts at:      0      92     133     226     458     737     878     985    1823 
   Size:         92      41      93     232     279     141     107     838

Cuts at:   1823    1948    2038    2144    2415 
   Size:        125      90     106     271

  Fragments arranged by size: 

                 838     279     271     232     141     125     107     106
                  93      92      90      41
 
Bsp1286I G_dGCh'C

Cuts at:      0      64      92     133     217     226     458     737     817 
   Size:         64      28      41      84       9     232     279      80

Cuts at:    817     850     878     985    1458    1823    1948    2038    2144 
   Size:         33      28     107     473     365     125      90     106

Cuts at:   2144    2415 
   Size:        271

  Fragments arranged by size: 

                 473     365     279     271     232     125     107     106
                  90      84      80      64      41      33      28      28
                   9
 
BspEI T'CCGG_A

Cuts at:      0    2051    2415 
   Size:       2051     364
 
BspHI T'CATG_A

Cuts at:      0    1373    2415 
   Size:       1373    1042
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1069    2010    2047    2415 
   Size:       1069     941      37     368

  Fragments arranged by size: 

                1069     941     368      37
 
BsrBI CCG'CTC

Cuts at:      0     168     365    2415 
   Size:        168     197    2050

  Fragments arranged by size: 

                2050     197     168
 
BsrDI GCAATG_nn'

Cuts at:      0    1763    2280    2415 
   Size:       1763     517     135

  Fragments arranged by size: 

                1763     517     135
 
BsrFI r'CCGG_y

Cuts at:      0     338     608    1649    1919    2077    2144    2415 
   Size:        338     270    1041     270     158      67     271

  Fragments arranged by size: 

                1041     338     271     270     270     158      67
 
BsrGI T'GTAC_A

Cuts at:      0    1076    2415 
   Size:       1076    1339
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     980    2386    2415 
   Size:        980    1406      29

  Fragments arranged by size: 

                1406     980      29
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     417     955    1896    2415 
   Size:        417     538     941     519

  Fragments arranged by size: 

                 941     538     519     417
 
Bsu36I CC'TnA_GG

Cuts at:      0     818    2415 
   Size:        818    1597
 
BtgI C'CryG_G

Cuts at:      0     410     685    1346    2415 
   Size:        410     275     661    1069

  Fragments arranged by size: 

                1069     661     410     275
 
BtsI GCAGTG_nn'

Cuts at:      0     476    2415 
   Size:        476    1939
 
EaeI y'GGCC_r

Cuts at:      0     227     245     270     606     921    1235    1349    1398 
   Size:        227      18      25     336     315     314     114      49

Cuts at:   1398    1478    1523    1553    1898    2198    2415 
   Size:         80      45      30     345     300     217

  Fragments arranged by size: 

                 345     336     315     314     300     227     217     114
                  80      49      45      30      25      18
 
EarI CTCTTCn'nnn_

Cuts at:      0     535    1732    1828    2415 
   Size:        535    1197      96     587

  Fragments arranged by size: 

                1197     587     535      96
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     357     373     654    1761    2415 
   Size:        357      16     281    1107     654

  Fragments arranged by size: 

                1107     654     357     281      16
 
EcoICRI GAG'CTC

Cuts at:      0     456     876    2415 
   Size:        456     420    1539

  Fragments arranged by size: 

                1539     456     420
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     418     439     442     711     721     892    1180 
   Size:        169     249      21       3     269      10     171     288

Cuts at:   1180    1243    1507    1531    1756    1801    2122    2415 
   Size:         63     264      24     225      45     321     293

  Fragments arranged by size: 

                 321     293     288     269     264     249     225     171
                 169      63      45      24      21      10       3
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     421     448    1198    2257    2415 
   Size:        421      27     750    1059     158

  Fragments arranged by size: 

                1059     750     421     158      27
 
EcoO109I rG'GnC_Cy

Cuts at:      0      60      61     548     675     814     847    1454    1455 
   Size:         60       1     487     127     139      33     607       1

Cuts at:   1455    2113    2415 
   Size:        658     302

  Fragments arranged by size: 

                 658     607     487     302     139     127      60      33
                   1       1
 
HaeII r_GCGC'y

Cuts at:      0     658     693    1915    1991    2415 
   Size:        658      35    1222      76     424

  Fragments arranged by size: 

                1222     658     424      76      35
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     272     304    1368    1400    2415 
   Size:        272      32    1064      32    1015

  Fragments arranged by size: 

                1064    1015     272      32      32
 
HindIII A'AGCT_T

Cuts at:      0    2308    2415 
   Size:       2308     107
 
KasI G'GCGC_C

Cuts at:      0    1911    1987    2415 
   Size:       1911      76     428

  Fragments arranged by size: 

                1911     428      76
 
KpnI G_GTAC'C

Cuts at:      0    2151    2415 
   Size:       2151     264
 
MscI TGG'CCA

Cuts at:      0     247    1400    1525    1555    1900    2415 
   Size:        247    1153     125      30     345     515

  Fragments arranged by size: 

                1153     515     345     247     125      30
 
MslI CAynn'nnrTG

Cuts at:      0     910    1072    2221    2415 
   Size:        910     162    1149     194

  Fragments arranged by size: 

                1149     910     194     162
 
MspA1I CmG'CkG

Cuts at:      0     480     584    1248    1993    2415 
   Size:        480     104     664     745     422

  Fragments arranged by size: 

                 745     664     480     422     104
 
NaeI GCC'GGC

Cuts at:      0     340     610    1921    2079    2415 
   Size:        340     270    1311     158     336

  Fragments arranged by size: 

                1311     340     336     270     158
 
NarI GG'CG_CC

Cuts at:      0    1912    1988    2415 
   Size:       1912      76     427

  Fragments arranged by size: 

                1912     427      76
 
NgoMIV G'CCGG_C

Cuts at:      0     338     608    1919    2077    2415 
   Size:        338     270    1311     158     338

  Fragments arranged by size: 

                1311     338     338     270     158
 
NspI r_CATG'y

Cuts at:      0     255     492    1017    2415 
   Size:        255     237     525    1398

  Fragments arranged by size: 

                1398     525     255     237
 
PasI CC'CwG_GG

Cuts at:      0      43     989    2117    2135    2415 
   Size:         43     946    1128      18     280

  Fragments arranged by size: 

                1128     946     280      43      18
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      12     465    1584    2415 
   Size:         12     453    1119     831

  Fragments arranged by size: 

                1119     831     453      12
 
PfoI T'CCnGG_A

Cuts at:      0     113    1778    2415 
   Size:        113    1665     637

  Fragments arranged by size: 

                1665     637     113
 
PpuMI rG'GwC_Cy

Cuts at:      0     548    2415 
   Size:        548    1867
 
PspOMI G'GGCC_C

Cuts at:      0      60     813     846    1454    2415 
   Size:         60     753      33     608     961

  Fragments arranged by size: 

                 961     753     608      60      33
 
PspXI vC'TCGA_Gb

Cuts at:      0    2031    2415 
   Size:       2031     384
 
PstI C_TGCA'G

Cuts at:      0     158     674    1534    1610    2415 
   Size:        158     516     860      76     805

  Fragments arranged by size: 

                 860     805     516     158      76
 
PvuII CAG'CTG

Cuts at:      0    1248    2415 
   Size:       1248    1167
 
SacI G_AGCT'C

Cuts at:      0     458     878    2415 
   Size:        458     420    1537

  Fragments arranged by size: 

                1537     458     420
 
SapI GCTCTTCn'nnn_

Cuts at:      0     535    1828    2415 
   Size:        535    1293     587

  Fragments arranged by size: 

                1293     587     535
 
SbfI CC_TGCA'GG

Cuts at:      0     674    1610    2415 
   Size:        674     936     805

  Fragments arranged by size: 

                 936     805     674
 
ScaI AGT'ACT

Cuts at:      0     301    2415 
   Size:        301    2114
 
SfcI C'TryA_G

Cuts at:      0     154     670     927    1530    1606    2415 
   Size:        154     516     257     603      76     809

  Fragments arranged by size: 

                 809     603     516     257     154      76
 
SfoI GGC'GCC

Cuts at:      0    1913    1989    2415 
   Size:       1913      76     426

  Fragments arranged by size: 

                1913     426      76
 
SmlI C'TyrA_G

Cuts at:      0     507     754    1546    2031    2257    2415 
   Size:        507     247     792     485     226     158

  Fragments arranged by size: 

                 792     507     485     247     226     158
 
SphI G_CATG'C

Cuts at:      0     255    2415 
   Size:        255    2160
 
SspI AAT'ATT

Cuts at:      0    1209    2415 
   Size:       1209    1206
 
StuI AGG'CCT

Cuts at:      0     869    2415 
   Size:        869    1546
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     232     916     985    1002    2079    2127    2415 
   Size:        232     684      69      17    1077      48     288

  Fragments arranged by size: 

                1077     684     288     232      69      48      17
 
TatI w'GTAC_w

Cuts at:      0     299     914    1076    2415 
   Size:        299     615     162    1339

  Fragments arranged by size: 

                1339     615     299     162
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     224     256    1693    1725    2415 
   Size:        224      32    1437      32     690

  Fragments arranged by size: 

                1437     690     224      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1669    2415 
   Size:       1669     746
 
XhoI C'TCGA_G

Cuts at:      0    2031    2415 
   Size:       2031     384
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI    AflII     AgeI     AleI    AlwNI     ApaI
    ApaLI     AvaI     BanI    BanII     BbeI     BbsI     BcgI     BglI
 Bme1580I    BmgBI     BmrI     BpmI    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI    BstXI   Bsu36I
     BtgI     BtsI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    HaeII    Hin4I  HindIII     KasI     KpnI     MscI     MslI
   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI    PflMI     PfoI
    PpuMI   PspOMI    PspXI     PstI    PvuII     SacI     SapI     SbfI
     ScaI     SfcI     SfoI     SmlI     SphI     SspI     StuI    TaqII
     TatI     TstI  Tth111I     XhoI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI   AflIII     AhdI     AlfI     AloI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI
    BciVI     BclI    BfrBI    BglII     BlpI     BmtI     BplI   Bpu10I
    BsaBI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI   BssHII    BssSI
    BstBI   BstEII    BstYI  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII     HpaI     MfeI     MluI     MmeI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI
     PpiI    PshAI     PsiI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     StyI     SwaI     XbaI     XcmI     XmaI     XmnI     ZraI
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