Restriction map for pF1KSDF0002
##################################
# NO CUT ENZYMES (   76 enzymes) #
##################################

AatII       AccIII      AclI        AflII       Alw44I      
ApaLI       AscI        AseI        AsnI        AspI        
AvrII       BbvCI       BcgI        BfrI        BglII       
BlnI        BsaBI       BseAI       BsiEI       BsiWI       
BsmBI       BspEI       BspHI       BspLU11I    BssHII      
BstBI       BstEII      BstPI       CpoI        DraI        
DraIII      DrdI        EagI        EclXI       Eco52I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FseI        
HpaI        KspI        MamI        MfeI        MluI        
MroI        MunI        NaeI        NdeI        NgoMIV      
NotI        NruI        NsiI        NspV        PacI        
PflFI       PmeI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspI        SwaI        Tth111I     
XbaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

Acc65I       1299  GGTACC               G^GTACC
AcsI         2931  AAATTC               [GA]^AATT[CT]
AflIII       2612  ACGTGT               A^C[GA][CT]GT
AgeI         1539  ACCGGT               A^CCGGT
AhdI         1484  GACTCCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      3490  AGCGCT               AGC^GCT
ApoI         2931  AAATTC               [GA]^AATT[CT]
Asp700I         4  GAACGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1299  GGTACC               G^GTACC
AspEI        1484  GACTCCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        4397  TGCGCA               TGC^GCA
BciVI        2126  GTATCC               GTATCC(6/5)
BclI         1670  TGATCA               T^GATCA
Bsp1407I     2081  TGTACA               T^GTACA
BspDI        4213  ATCGAT               AT^CGAT
BsrGI        2081  TGTACA               T^GTACA
Bst1107I     1519  GTATAC               GTA^TAC
BstAPI       4425  GCAGCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        3932  CCAGCCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1519  GTATAC               GTA^TAC
ClaI         4213  ATCGAT               AT^CGAT
Eam1105I     1484  GACTCCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     3490  AGCGCT               AGC^GCT
FbaI         1670  TGATCA               T^GATCA
FspI         4397  TGCGCA               TGC^GCA
HincII       1306  GTCAAC               GT[CT]^[GA]AC
HindII       1306  GTCAAC               GT[CT]^[GA]AC
KpnI         1299  GGTACC               GGTAC^C
NheI          987  GCTAGC               G^CTAGC
PaeR7I       1469  CTCGAG               C^TCGAG
PinAI        1539  ACCGGT               A^CCGGT
PshAI        2477  GACAAGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmaI          389  CCCGGG               CCC^GGG
SphI         2312  GCATGC               GCATG^C
SspBI        2081  TGTACA               T^GTACA
Tsp509I      2932  AATT                 ^AATT
XhoI         1469  CTCGAG               C^TCGAG
XmaCI         389  CCCGGG               C^CCGGG
XmaI          389  CCCGGG               C^CCGGG
XmnI            4  GAACGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccI         1519  GTATAC               GT^[AC][GT]AC
             2218  GTCTAC              
AocI          631  CCTGAGG              CC^T[ACGT]AGG
              730  CCTCAGG             
BbrPI        2611  CACGTG               CAC^GTG
             3238  CACGTG              
BlpI           99  GCTCAGC              GC^T[ACGT]AGC
             2316  GCTGAGC             
Bpu1102I       99  GCTCAGC              GC^T[ACGT]AGC
             2316  GCTGAGC             
BsaAI        2611  CACGTG               [CT]AC^GT[GA]
             3238  CACGTG              
BsaWI        1539  ACCGGT               [AT]^CCGG[AT]
             1706  ACCGGA              
BsrBI          46  CCGCTC               CCGCTC(-3/-3)
             3646  GAGCGG              
Bsu36I        631  CCTGAGG              CC^T[ACGT]AGG
              730  CCTCAGG             
CelII          99  GCTCAGC              GC^T[ACGT]AGC
             2316  GCTGAGC             
Eco81I        631  CCTGAGG              CC^T[ACGT]AGG
              730  CCTCAGG             
HgaI         2374  GACGC                GACGC(5/10)
             3641  GCGTC               
HindIII      1378  AAGCTT               A^AGCTT
             2621  AAGCTT              
MseI         1175  TTAA                 T^TAA
             1376  TTAA                
NcoI          503  CCATGG               C^CATGG
             4100  CCATGG              
NspI         1965  GCATGT               [GA]CATG^[CT]
             2312  GCATGC              
PmaCI        2611  CACGTG               CAC^GTG
             3238  CACGTG              
PmlI         2611  CACGTG               CAC^GTG
             3238  CACGTG              
SfiI          699  GGCCCGTGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             3354  GGCCACAGGGGCC       
SmlI         1469  CTCGAG               C^T[CT][GA]AG
             3400  CTTGAG              
Tru9I        1175  TTAA                 T^TAA
             1376  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BalI        BamHI       BanI        BanII       
BbeI        BbsI        BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BpuAI       BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspMI       BsrDI       BsrFI       
BsrI        BssKI       BssSI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NarI        NciI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SacI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
StuI        StyI        TaqI        TfiI        TseI        
Tsp45I      TspRI       TthHB8I     Van91I      XcmI        
XhoII       
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