Restriction map for pF1KB9404
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspHI       
AspI        AviII       AvrII       BalI        BanI        
BbeI        BbrPI       BbvCI       BciVI       BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaI        BseAI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstEII      BstPI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraIII      DrdI        DsaI        EaeI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FseI        FspI        HaeII       
HpaI        KasI        KpnI        KspI        MluI        
MluNI       MroI        MscI        MspA1I      NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PstI        PvuI        PvuII       
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        StyI        SwaI        Tth111I     
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI          974  GTAGAC               GT^[AC][GT]AC
AccII         218  CGCG                 CG^CG
AcsI          402  GAATTT               [GA]^AATT[CT]
AcyI         1751  GACGCC               G[GA]^CG[CT]C
AflIII       1878  ACATGT               A^C[GA][CT]GT
AhdI         2120  GACAGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI          402  GAATTT               [GA]^AATT[CT]
AspEI        2120  GACAGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          896  CTCGGG               C^[CT]CG[GA]G
BclI          966  TGATCA               T^GATCA
BpmI         2024  CTGGAG               CTGGAG(16/14)
BsaHI        1751  GACGCC               G[GA]^CG[CT]C
BsaWI        1611  ACCGGA               [AT]^CCGG[AT]
BsgI          992  GTGCAG               GTGCAG(16/14)
BsoBI         896  CTCGGG               C^[CT]CG[GA]G
BspHI        1968  TCATGA               T^CATGA
BspLU11I     1878  ACATGT               A^CATGT
BspMI         246  GCAGGT               ACCTGC(4/8)
BstAPI        285  GCAGAGTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1289  TTCGAA               TT^CGAA
BstUI         218  CGCG                 CG^CG
BstXI        1130  CCATTGTATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          219  GCGC                 GCG^C
DraII        2141  AGGACCT              [GA]G^G[ACGT]CC[CT]
Eam1105I     2120  GACAGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I     2141  AGGACCT              [GA]G^G[ACGT]CC[CT]
FbaI          966  TGATCA               T^GATCA
HaeIII        710  GGCC                 GG^CC
HgaI         1751  GACGC                GACGC(5/10)
HhaI          219  GCGC                 GCG^C
Hin1I        1751  GACGCC               G[GA]^CG[CT]C
HinP1I        219  GCGC                 G^CGC
HincII        436  GTCAAC               GT[CT]^[GA]AC
HindII        436  GTCAAC               GT[CT]^[GA]AC
HindIII      1669  AAGCTT               A^AGCTT
MaeII        2379  ACGT                 A^CGT
MvnI          218  CGCG                 CG^CG
NspV         1289  TTCGAA               TT^CGAA
PflMI         772  CCACTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        2141  AGGACCT              [GA]G^G[AT]CC[CT]
RcaI         1968  TCATGA               T^CATGA
SapI          763  GCTCTTC              GCTCTTC(1/4)
SfcI          987  CTACAG               C^T[GA][CT]AG
SfuI         1289  TTCGAA               TT^CGAA
SspI          980  AATATT               AAT^ATT
Tsp45I        214  GTCAC                ^GT[GC]AC
Van91I        772  CCACTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

Asp700I      2005  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2344  GAATTAATTC          
BamHI        1659  GGATCC               G^GATCC
             1807  GGATCC              
BanII         783  GAGCCC               G[GA]GC[CT]^C
             2027  GAGCCC              
BbsI         1197  GTCTTC               GAAGAC(2/6)
             1305  GTCTTC              
BcgI          275  CGAAAATAATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1764  GCAAGCTGTTCG        
BcnI          541  CCCGG                CC^[GC]GG
              786  CCCGG               
BfaI          572  CTAG                 C^TAG
             1691  CTAG                
BmyI          783  GAGCCC               G[AGT]GC[ACT]^C
             2027  GAGCCC              
BpuAI        1197  GTCTTC               GAAGAC(2/6)
             1305  GTCTTC              
BsaBI         391  GATGCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1225  GATACAGATC          
BsmAI        1119  GAGAC                GTCTC(1/5)
             2296  GAGAC               
BsmFI         859  GGGAC                GGGAC(10/14)
             2270  GTCCC               
Bsp1286I      783  GAGCCC               G[AGT]GC[ACT]^C
             2027  GAGCCC              
DraI         1543  TTTAAA               TTT^AAA
             1681  TTTAAA              
MaeI          572  CTAG                 C^TAG
             1691  CTAG                
MaeIII        214  GTCAC                ^GT[ACGT]AC
             1258  GTAAC               
MamI          391  GATGCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1225  GATACAGATC          
MfeI         1427  CAATTG               C^AATTG
             2258  CAATTG              
MunI         1427  CAATTG               C^AATTG
             2258  CAATTG              
MwoI          153  GCAAGTCAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              285  GCAGAGTTTGC         
NciI          541  CCCGG                CC^[GC]GG
              786  CCCGG               
NspI          957  GCATGT               [GA]CATG^[CT]
             1878  ACATGT              
XmnI         2005  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2344  GAATTAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AseI        
AsnI        AvaII       BbvI        BsaJI       BseRI       
BsiYI       BslI        BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       EarI        EcoRII      Fnu4HI      FokI        
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MboI        MboII       MflI        MnlI        
MseI        MslI        MspI        MvaI        NdeII       
NlaIII      NlaIV       PleI        PshBI       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
TaqI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com