Restriction map for pF1KB9403
##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

AccI        AclI        AflII       AflIII      AgeI        
AhdI        AscI        AseI        AsnI        AspEI       
BamHI       BbrPI       BciVI       BclI        BfrI        
BsaBI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspLU11I    BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
CpoI        DraI        Eam1105I    EcoO65I     EcoRV       
EcoT22I     FbaI        FseI        HindIII     HpaI        
MamI        MfeI        MluI        MunI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    RsrII       SalI        
ScaI        SexAI       SfuI        SgrAI       SmaI        
SnaBI       SpeI        SspBI       SwaI        XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AatII        2074  GACGTC               GACGT^C
Alw44I        700  GTGCAC               G^TGCAC
ApaLI         700  GTGCAC               G^TGCAC
Asp700I       286  GAATTCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        1491  TGCGCA               TGC^GCA
AvrII        3006  CCTAGG               C^CTAGG
BbeI          202  GGCGCC               GGCGC^C
BlnI         3006  CCTAGG               C^CTAGG
BlpI         2284  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2284  GCTGAGC              GC^T[ACGT]AGC
BspHI         930  TCATGA               T^CATGA
BsrDI        3096  CATTGC               GCAATG(2/0)
BsrFI        3332  GCCGGC               [GA]^CCGG[CT]
CelII        2284  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       3332  GCCGGC               [GA]^CCGG[CT]
DraIII       1554  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         2068  GACACTGACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         3334  CGGCCG               C^GGCCG
EclXI        3334  CGGCCG               C^GGCCG
Eco52I       3334  CGGCCG               C^GGCCG
FspI         1491  TGCGCA               TGC^GCA
KasI          202  GGCGCC               G^GCGCC
KspI          171  CCGCGG               CCGC^GG
NaeI         3332  GCCGGC               GCC^GGC
NarI          202  GGCGCC               GG^CGCC
NcoI         1901  CCATGG               C^CATGG
NgoMIV       3332  GCCGGC               G^CCGGC
PvuI          856  CGATCG               CGAT^CG
RcaI          930  TCATGA               T^CATGA
SacI         2287  GAGCTC               GAGCT^C
SacII         171  CCGCGG               CCGC^GG
SbfI         2373  CCTGCAGG             CCTGCA^GG
SfoI          202  GGCGCC               GGC^GCC
SphI         1667  GCATGC               GCATG^C
Sse8387I     2373  CCTGCAGG             CCTGCA^GG
SspI          683  AATATT               AAT^ATT
XmnI          286  GAATTCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccIII       2514  TCCGGA               T^CCGGA
             3116  TCCGGA              
AcsI          286  GAATTC               [GA]^AATT[CT]
             2489  GAATTC              
AcyI          202  GGCGCC               G[GA]^CG[CT]C
             2074  GACGTC              
AocI          661  CCTGAGG              CC^T[ACGT]AGG
             3674  CCTCAGG             
ApaI         1319  GGGCCC               GGGCC^C
             3018  GGGCCC              
ApoI          286  GAATTC               [GA]^AATT[CT]
             2489  GAATTC              
BalI          981  TGGCCA               TGG^CCA
             1380  TGGCCA              
BglII        2430  AGATCT               A^GATCT
             3348  AGATCT              
BsaHI         202  GGCGCC               G[GA]^CG[CT]C
             2074  GACGTC              
BseAI        2514  TCCGGA               T^CCGGA
             3116  TCCGGA              
BsmI         1184  GAATGC               GAATGC(1/-1)
              241  GCATTC              
BspEI        2514  TCCGGA               T^CCGGA
             3116  TCCGGA              
Bsu36I        661  CCTGAGG              CC^T[ACGT]AGG
             3674  CCTCAGG             
Eco81I        661  CCTGAGG              CC^T[ACGT]AGG
             3674  CCTCAGG             
EcoNI        1720  CCTCCCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2022  CCTTCTCAAGG         
EcoRI         286  GAATTC               G^AATTC
             2489  GAATTC              
Hin1I         202  GGCGCC               G[GA]^CG[CT]C
             2074  GACGTC              
HincII        136  GTCAAC               GT[CT]^[GA]AC
              997  GTCAAC              
HindII        136  GTCAAC               GT[CT]^[GA]AC
              997  GTCAAC              
MluNI         981  TGGCCA               TGG^CCA
             1380  TGGCCA              
MroI         2514  TCCGGA               T^CCGGA
             3116  TCCGGA              
MscI          981  TGGCCA               TGG^CCA
             1380  TGGCCA              
MseI          692  TTAA                 T^TAA
             2144  TTAA                
NdeI          316  CATATG               CA^TATG
             1199  CATATG              
NspI         1667  GCATGC               [GA]CATG^[CT]
             3653  GCATGT              
PflMI        2115  CCAGGACTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2220  CCAGCACTTGG         
SfiI         1138  GGCCCAAGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             3759  GGCCTCCGAGGCC       
StuI         2460  AGGCCT               AGG^CCT
             3767  AGGCCT              
Tru9I         692  TTAA                 T^TAA
             2144  TTAA                
Van91I       2115  CCAGGACTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2220  CCAGCACTTGG         
XcmI          482  CCAATGTTCCCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1892  CCATGCCCTCCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

Acc65I      AccII       AciI        AfaI        AluI        
AlwI        AlwNI       Aor51HI     Asp718I     AspHI       
AspI        AvaI        AvaII       BanI        BanII       
BbsI        BbvCI       BbvI        BcgI        BcnI        
BfaI        BglI        BmyI        BpmI        BpuAI       
BsaAI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        Eco47III    EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
KpnI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PflFI       
PleI        PpuMI       PstI        PvuII       RsaI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     XhoII       
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