Restriction map for pF1KSDB3800
##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AatII       AccIII      AcyI        AflII       AgeI        
AhdI        Alw44I      Aor51HI     ApaLI       AscI        
AseI        AsnI        AspEI       AvaI        AviII       
BbeI        BbrPI       BbvCI       BcnI        BfrI        
BglI        BsaHI       BseAI       BsiEI       BsiWI       
BsoBI       BspDI       BspEI       BspLU11I    BsrBI       
BssHII      BssSI       BstBI       ClaI        CpoI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       FauI        FseI        FspI        
Hin1I       KasI        KspI        MluI        MroI        
MspA1I      NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       PvuI        PvuII       RsrII       
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SwaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccI         3045  GTATAC               GT^[AC][GT]AC
AccII        3462  CGCG                 CG^CG
AclI         3514  AACGTT               AA^CGTT
AflIII       3786  ACGTGT               A^C[GA][CT]GT
ApaI         3921  GGGCCC               GGGCC^C
AspI         2278  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII         592  CCTAGG               C^CTAGG
BalI         3834  TGGCCA               TGG^CCA
BamHI         333  GGATCC               G^GATCC
BbsI           28  GAAGAC               GAAGAC(2/6)
BciVI        3334  GGATAC               GTATCC(6/5)
BlnI          592  CCTAGG               C^CTAGG
BlpI         2152  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2152  GCTCAGC              GC^T[ACGT]AGC
BpuAI          28  GAAGAC               GAAGAC(2/6)
BsaAI        3785  TACGTG               [CT]AC^GT[GA]
BsaI          455  GAGACC               GGTCTC(1/5)
BseRI         667  GAGGAG               GAGGAG(10/8)
BsgI         2692  GTGCAG               GTGCAG(16/14)
Bsp1407I     3219  TGTACA               T^GTACA
BspHI        3011  TCATGA               T^CATGA
BspMI        1027  GCAGGT               ACCTGC(4/8)
BsrFI        3885  GCCGGT               [GA]^CCGG[CT]
BsrGI        3219  TGTACA               T^GTACA
Bst1107I     3045  GTATAC               GTA^TAC
BstEII       1309  GGTGACC              G^GT[ACGT]ACC
BstPI        1309  GGTGACC              G^GT[ACGT]ACC
BstUI        3462  CGCG                 CG^CG
BstZ17I      3045  GTATAC               GTA^TAC
CelII        2152  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       3885  GCCGGT               [GA]^CCGG[CT]
DraIII       2686  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         3834  TGGCCA               [CT]^GGCC[GA]
EcoO65I      1309  GGTGACC              G^GT[ACGT]ACC
EcoRV        2674  GATATC               GAT^ATC
HgaI         3460  GACGC                GACGC(5/10)
HindIII      3716  AAGCTT               A^AGCTT
HpaI          370  GTTAAC               GTT^AAC
MfeI         3663  CAATTG               C^AATTG
MluNI        3834  TGGCCA               TGG^CCA
MscI         3834  TGGCCA               TGG^CCA
MunI         3663  CAATTG               C^AATTG
MvnI         3462  CGCG                 CG^CG
PacI           37  TTAATTAA             TTAAT^TAA
PflFI        2278  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         566  AGGACCT              [GA]G^G[AT]CC[CT]
Psp1406I     3514  AACGTT               AA^CGTT
RcaI         3011  TCATGA               T^CATGA
ScaI         2832  AGTACT               AGT^ACT
SphI         2604  GCATGC               GCATG^C
SspBI        3219  TGTACA               T^GTACA
SspI         1246  AATATT               AAT^ATT
StuI         2072  AGGCCT               AGG^CCT
Tth111I      2278  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1032  TCTAGA               T^CTAGA
XcmI          510  CCAGGGAACAGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

Acc65I       1782  GGTACC               G^GTACC
             2112  GGTACC              
AciI         3457  GCGG                 CCGC(-3/-1)
             3463  GCGG                
AocI          976  CCTGAGG              CC^T[ACGT]AGG
             2075  CCTCAGG             
Asp718I      1782  GGTACC               G^GTACC
             2112  GGTACC              
AvaII         567  GGACC                G^G[AT]CC
             3375  GGTCC               
BcgI         2694  GCAGCTTTATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3521  GCAAGGGTGTCG        
BclI           63  TGATCA               T^GATCA
              270  TGATCA              
BsaBI        1702  GATAATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2862  GATTCTTATC          
BsmBI        2317  GAGACG               CGTCTC(1/5)
             2995  GAGACG              
BsmFI        2739  GGGAC                GGGAC(10/14)
             3780  GGGAC               
BsrDI        2377  GCAATG               GCAATG(2/0)
             3830  GCAATG              
BstAPI       3172  GCAGGAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3411  GCAGGCACTGC         
BstXI        2102  CCACTGTGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3350  CCAGTATTGTGG        
Bsu36I        976  CCTGAGG              CC^T[ACGT]AGG
             2075  CCTCAGG             
CfoI         3314  GCGC                 GCG^C
             3875  GCGC                
EarI         3327  CTCTTC               CTCTTC(1/4)
             2452  GAAGAG              
Eco81I        976  CCTGAGG              CC^T[ACGT]AGG
             2075  CCTCAGG             
EcoRI         315  GAATTC               G^AATTC
              847  GAATTC              
EcoT22I       154  ATGCAT               ATGCA^T
             2602  ATGCAT              
FbaI           63  TGATCA               T^GATCA
              270  TGATCA              
HaeII        3313  GGCGCT               [GA]GCGC^[CT]
             3874  AGCGCC              
HhaI         3314  GCGC                 GCG^C
             3875  GCGC                
HinP1I       3314  GCGC                 G^CGC
             3875  GCGC                
HincII        370  GTTAAC               GT[CT]^[GA]AC
              903  GTTGAC              
HindII        370  GTTAAC               GT[CT]^[GA]AC
              903  GTTGAC              
KpnI         1782  GGTACC               GGTAC^C
             2112  GGTACC              
Ksp632I      3327  CTCTTC               CTCTTC(1/4)
             2452  GAAGAG              
MaeII        3515  ACGT                 A^CGT
             3786  ACGT                
MamI         1702  GATAATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2862  GATTCTTATC          
NsiI          154  ATGCAT               ATGCA^T
             2602  ATGCAT              
PflMI        2983  CCATTGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3492  CCAGTGGGTGG         
SacI         2081  GAGCTC               GAGCT^C
             2088  GAGCTC              
TaqI         1619  TCGA                 T^CGA
             2940  TCGA                
TthHB8I      1619  TCGA                 T^CGA
             2940  TCGA                
Van91I       2983  CCATTGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3492  CCAGTGGGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        AlwNI       
ApoI        Asp700I     AspHI       BanI        BanII       
BbvI        BfaI        BglII       BmyI        BpmI        
BsaJI       BsaWI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmI        Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspI        
MvaI        MwoI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XhoII       XmnI        
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