Restriction map for pF1KSDB3800
 (Linear) (Six-base) MAPSORT of: KIBB3800.seq  Check: 89  from: 1  to: 3969



KIBB3800.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:19  ..

AccI GT'mk_AC

Cuts at:      0    3046    3969 
   Size:       3046     923
 
Acc65I G'GTAC_C

Cuts at:      0    1782    2112    3969 
   Size:       1782     330    1857

  Fragments arranged by size: 

                1857    1782     330
 
AclI AA'CG_TT

Cuts at:      0    3515    3969 
   Size:       3515     454
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     701    2414    2736    3015    3775    3969 
   Size:        701    1713     322     279     760     194

  Fragments arranged by size: 

                1713     760     701     322     279     194
 
AflIII A'CryG_T

Cuts at:      0    3786    3969 
   Size:       3786     183
 
AleI CACnn'nnGTG

Cuts at:      0     632    3969 
   Size:        632    3337
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3385    3417    3969 
   Size:       3385      32     552

  Fragments arranged by size: 

                3385     552      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     413    2820    3417    3969 
   Size:        413    2407     597     552

  Fragments arranged by size: 

                2407     597     552     413
 
ApaI G_GGCC'C

Cuts at:      0    3925    3969 
   Size:       3925      44
 
ApoI r'AATT_y

Cuts at:      0     315     615     847    1463    1730    2532    3107    3969 
   Size:        315     300     232     616     267     802     575     862

  Fragments arranged by size: 

                 862     802     616     575     315     300     267     232
            
 
AvrII C'CTAG_G

Cuts at:      0     592    3969 
   Size:        592    3377
 
BamHI G'GATC_C

Cuts at:      0     333    3969 
   Size:        333    3636
 
BanI G'GyrC_C

Cuts at:      0    1782    2112    3178    3289    3882    3969 
   Size:       1782     330    1066     111     593      87

  Fragments arranged by size: 

                1782    1066     593     330     111      87
 
BanII G_rGCy'C

Cuts at:      0    2014    2085    2092    3925    3969 
   Size:       2014      71       7    1833      44

  Fragments arranged by size: 

                2014    1833      71      44       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      35    3969 
   Size:         35    3934
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2683    2717    3510    3544    3969 
   Size:       2683      34     793      34     425

  Fragments arranged by size: 

                2683     793     425      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3328    3969 
   Size:       3328     641
 
BclI T'GATC_A

Cuts at:      0      63     270    3969 
   Size:         63     207    3699

  Fragments arranged by size: 

                3699     207      63
 
BfrBI ATG'CAT

Cuts at:      0     156    2604    3969 
   Size:        156    2448    1365

  Fragments arranged by size: 

                2448    1365     156
 
BglII A'GATC_T

Cuts at:      0     364     600    1658    3969 
   Size:        364     236    1058    2311

  Fragments arranged by size: 

                2311    1058     364     236
 
BlpI GC'TnA_GC

Cuts at:      0    2153    3969 
   Size:       2153    1816
 
Bme1580I G_kGCm'C

Cuts at:      0    3925    3969 
   Size:       3925      44
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2275    3787    3969 
   Size:       2275    1512     182

  Fragments arranged by size: 

                2275    1512     182
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     888    2366    3969 
   Size:        888    1478    1603

  Fragments arranged by size: 

                1603    1478     888
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1673    1757    2159    3969 
   Size:       1673      84     402    1810

  Fragments arranged by size: 

                1810    1673     402      84
 
Bpu10I CC'TnA_GC

Cuts at:      0    2069    3969 
   Size:       2069    1900
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1958    1986    3426    3969 
   Size:       1958      28    1440     543

  Fragments arranged by size: 

                1958    1440     543      28
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     449    3969 
   Size:        449    3520
 
BsaAI yAC'GTr

Cuts at:      0    3787    3969 
   Size:       3787     182
 
BsaBI GATnn'nnATC

Cuts at:      0    1706    2866    3969 
   Size:       1706    1160    1103

  Fragments arranged by size: 

                1706    1160    1103
 
BsaWI w'CCGG_w

Cuts at:      0     692    1785    2649    3969 
   Size:        692    1093     864    1320

  Fragments arranged by size: 

                1320    1093     864     692
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2037    2067    2132    2162    3969 
   Size:       2037      30      65      30    1807

  Fragments arranged by size: 

                2037    1807      65      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     682    3969 
   Size:        682    3287
 
BseYI C'CCAG_C

Cuts at:      0    3112    3969 
   Size:       3112     857
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2713    3969 
   Size:       2713    1256
 
BsiHKAI G_wGCw'C

Cuts at:      0     676    2085    2092    3482    3969 
   Size:        676    1409       7    1390     487

  Fragments arranged by size: 

                1409    1390     676     487       7
 
BsmI GAATG_Cn'

Cuts at:      0    1145    1240    1974    2606    3969 
   Size:       1145      95     734     632    1363

  Fragments arranged by size: 

                1363    1145     734     632      95
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2311    2989    3969 
   Size:       2311     678     980

  Fragments arranged by size: 

                2311     980     678
 
Bsp1286I G_dGCh'C

Cuts at:      0     676    2014    2085    2092    3482    3925    3969 
   Size:        676    1338      71       7    1390     443      44

  Fragments arranged by size: 

                1390    1338     676     443      71      44       7
 
BspHI T'CATG_A

Cuts at:      0    3011    3969 
   Size:       3011     958
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1018    3969 
   Size:       1018    2951
 
BsrDI GCAATG_nn'

Cuts at:      0    2384    3837    3969 
   Size:       2384    1453     132

  Fragments arranged by size: 

                2384    1453     132
 
BsrFI r'CCGG_y

Cuts at:      0    3885    3969 
   Size:       3885      84
 
BsrGI T'GTAC_A

Cuts at:      0    3219    3969 
   Size:       3219     750
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3178    3417    3969 
   Size:       3178     239     552

  Fragments arranged by size: 

                3178     552     239
 
BstEII G'GTnAC_C

Cuts at:      0    1309    3969 
   Size:       1309    2660
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2109    3357    3969 
   Size:       2109    1248     612

  Fragments arranged by size: 

                2109    1248     612
 
BstYI r'GATC_y

Cuts at:      0     333     364     600    1658    2192    3969 
   Size:        333      31     236    1058     534    1777

  Fragments arranged by size: 

                1777    1058     534     333     236      31
 
BstZ17I GTA'TAC

Cuts at:      0    3047    3969 
   Size:       3047     922
 
Bsu36I CC'TnA_GG

Cuts at:      0     977    2076    3969 
   Size:        977    1099    1893

  Fragments arranged by size: 

                1893    1099     977
 
BtgI C'CryG_G

Cuts at:      0    3554    3585    3894    3969 
   Size:       3554      31     309      75

  Fragments arranged by size: 

                3554     309      75      31
 
BtsI GCAGTG_nn'

Cuts at:      0      78    1851    2615    2818    3415    3969 
   Size:         78    1773     764     203     597     554

  Fragments arranged by size: 

                1773     764     597     554     203      78
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2834    2869    3969 
   Size:       2834      35    1100

  Fragments arranged by size: 

                2834    1100      35
 
DraI TTT'AAA

Cuts at:      0    1040    1298    2129    2525    3969 
   Size:       1040     258     831     396    1444

  Fragments arranged by size: 

                1444    1040     831     396     258
 
DraIII CAC_nnn'GTG

Cuts at:      0    2691    3969 
   Size:       2691    1278
 
EaeI y'GGCC_r

Cuts at:      0    3834    3969 
   Size:       3834     135
 
EarI CTCTTCn'nnn_

Cuts at:      0    2447    3333    3969 
   Size:       2447     886     636

  Fragments arranged by size: 

                2447     886     636
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3472    3969 
   Size:       3472     497
 
EcoICRI GAG'CTC

Cuts at:      0    2083    2090    3969 
   Size:       2083       7    1879

  Fragments arranged by size: 

                2083    1879       7
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     701    1673    1757    2159    2414    2736    3015    3775 
   Size:        701     972      84     402     255     322     279     760

Cuts at:   3775    3969 
   Size:        194

  Fragments arranged by size: 

                 972     760     701     402     322     279     255     194
                  84
 
EcoO109I rG'GnC_Cy

Cuts at:      0     567    3902    3921    3922    3969 
   Size:        567    3335      19       1      47

  Fragments arranged by size: 

                3335     567      47      19       1
 
EcoRI G'AATT_C

Cuts at:      0     315     847    3969 
   Size:        315     532    3122

  Fragments arranged by size: 

                3122     532     315
 
EcoRV GAT'ATC

Cuts at:      0    2676    3969 
   Size:       2676    1293
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     850    2419    2694    3969 
   Size:        850    1569     275    1275

  Fragments arranged by size: 

                1569    1275     850     275
 
HaeII r_GCGC'y

Cuts at:      0    3317    3878    3969 
   Size:       3317     561      91

  Fragments arranged by size: 

                3317     561      91
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     349     381    2446    2478    3535    3567    3969 
   Size:        349      32    2065      32    1057      32     402

  Fragments arranged by size: 

                2065    1057     402     349      32      32      32
 
HincII GTy'rAC

Cuts at:      0     372     905    3969 
   Size:        372     533    3064

  Fragments arranged by size: 

                3064     533     372
 
HindIII A'AGCT_T

Cuts at:      0    3716    3969 
   Size:       3716     253
 
HpaI GTT'AAC

Cuts at:      0     372    3969 
   Size:        372    3597
 
KpnI G_GTAC'C

Cuts at:      0    1786    2116    3969 
   Size:       1786     330    1853

  Fragments arranged by size: 

                1853    1786     330
 
MfeI C'AATT_G

Cuts at:      0    3663    3969 
   Size:       3663     306
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     993    2337    2349    2468    3969 
   Size:        993    1344      12     119    1501

  Fragments arranged by size: 

                1501    1344     993     119      12
 
MscI TGG'CCA

Cuts at:      0    3836    3969 
   Size:       3836     133
 
MslI CAynn'nnrTG

Cuts at:      0     161     632    2107    3969 
   Size:        161     471    1475    1862

  Fragments arranged by size: 

                1862    1475     471     161
 
NcoI C'CATG_G

Cuts at:      0    3554    3585    3894    3969 
   Size:       3554      31     309      75

  Fragments arranged by size: 

                3554     309      75      31
 
NsiI A_TGCA'T

Cuts at:      0     158    2606    3969 
   Size:        158    2448    1363

  Fragments arranged by size: 

                2448    1363     158
 
NspI r_CATG'y

Cuts at:      0     729    1865    2608    3166    3709    3969 
   Size:        729    1136     743     558     543     260

  Fragments arranged by size: 

                1136     743     729     558     543     260
 
PacI TTA_AT'TAA

Cuts at:      0      41    3969 
   Size:         41    3928
 
PasI CC'CwG_GG

Cuts at:      0     510    3969 
   Size:        510    3459
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2989    3498    3969 
   Size:       2989     509     471

  Fragments arranged by size: 

                2989     509     471
 
PpuMI rG'GwC_Cy

Cuts at:      0     567    3969 
   Size:        567    3402
 
PsiI TTA'TAA

Cuts at:      0     209    3969 
   Size:        209    3760
 
PspOMI G'GGCC_C

Cuts at:      0    3921    3969 
   Size:       3921      48
 
PstI C_TGCA'G

Cuts at:      0    1858    2256    3390    3684    3969 
   Size:       1858     398    1134     294     285

  Fragments arranged by size: 

                1858    1134     398     294     285
 
SacI G_AGCT'C

Cuts at:      0    2085    2092    3969 
   Size:       2085       7    1877

  Fragments arranged by size: 

                2085    1877       7
 
ScaI AGT'ACT

Cuts at:      0    2834    3969 
   Size:       2834    1135
 
SfcI C'TryA_G

Cuts at:      0      51     842     961    1344    1854    1890    2252    2638 
   Size:         51     791     119     383     510      36     362     386

Cuts at:   2638    3386    3539    3680    3969 
   Size:        748     153     141     289

  Fragments arranged by size: 

                 791     748     510     386     383     362     289     153
                 141     119      51      36
 
SmlI C'TyrA_G

Cuts at:      0    1973    2001    3405    3969 
   Size:       1973      28    1404     564

  Fragments arranged by size: 

                1973    1404     564      28
 
SphI G_CATG'C

Cuts at:      0    2608    3969 
   Size:       2608    1361
 
SspI AAT'ATT

Cuts at:      0    1248    3969 
   Size:       1248    2721
 
StuI AGG'CCT

Cuts at:      0    2074    3969 
   Size:       2074    1895
 
StyI C'CwwG_G

Cuts at:      0     592    1382    2594    2766    2887    3554    3585    3878 
   Size:        592     790    1212     172     121     667      31     293

Cuts at:   3878    3894    3969 
   Size:         16      75

  Fragments arranged by size: 

                1212     790     667     592     293     172     121      75
                  31      16
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     363    1750    3486    3969 
   Size:        363    1387    1736     483

  Fragments arranged by size: 

                1736    1387     483     363
 
TatI w'GTAC_w

Cuts at:      0    2832    3219    3854    3969 
   Size:       2832     387     635     115

  Fragments arranged by size: 

                2832     635     387     115
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2132    2164    2644    2676    3773    3805    3969 
   Size:       2132      32     480      32    1097      32     164

  Fragments arranged by size: 

                2132    1097     480     164      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2281    3969 
   Size:       2281    1688
 
XbaI T'CTAG_A

Cuts at:      0    1032    3969 
   Size:       1032    2937
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     517    3969 
   Size:        517    3452
 
XmnI GAAnn'nnTTC

Cuts at:      0     956    1873    3433    3580    3969 
   Size:        956     917    1560     147     389

  Fragments arranged by size: 

                1560     956     917     389     147
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AleI     AloI    AlwNI
     ApaI     ApoI    AvrII    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI
    BspMI    BsrDI    BsrFI    BsrGI   BstAPI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI     BtsI    CspCI     DraI   DraIII     EaeI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MmeI
     MscI     MslI     NcoI     NsiI     NspI     PacI     PasI    PflMI
    PpuMI     PsiI   PspOMI     PstI     SacI     ScaI     SfcI     SmlI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AhdI     AlfI    ApaLI
     AscI     AseI    AsiSI     AvaI     BaeI     BbeI    BbvCI     BglI
    BmgBI     BmtI    BsaHI    BsiEI    BsiWI    BspEI    BsrBI   BssHII
    BssSI    BstBI    BtgZI     ClaI     DrdI     EagI    EcoNI     FseI
     FspI    FspAI     KasI     MluI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     PciI     PfoI     PmeI     PmlI
     PpiI    PshAI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SwaI     XhoI     XmaI
     ZraI
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