Restriction map for pF1KSDB1453
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

Acc65I      AccI        AccII       AclI        AgeI        
AhdI        Alw44I      ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AvaI        
AvaII       AviII       AvrII       BamHI       BanI        
BbeI        BbrPI       BbvCI       BciVI       BglI        
BglII       BlnI        BlpI        Bpu1102I    BsgI        
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspHI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco52I      EcoNI       EcoO65I     
EcoRV       EcoT22I     FauI        FseI        FspI        
HgaI        HindIII     HpaI        KasI        KpnI        
KspI        MfeI        MluI        MspA1I      MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NlaIV       NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Van91I      
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   65 enzymes) #
###################################

AatII         824  GACGTC               GACGT^C
AccIII        191  TCCGGA               T^CCGGA
AciI          406  GCGG                 CCGC(-3/-1)
AcyI          824  GACGTC               G[GA]^CG[CT]C
AflII        1255  CTTAAG               C^TTAAG
AlwI         1062  GGATC                GGATC(4/5)
AlwNI        1241  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          871  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1337  AGCGCT               AGC^GCT
Asp700I       450  GAAAATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        1106  GAGCTC               G[AT]GC[AT]^C
AspI         1309  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanII        1106  GAGCTC               G[GA]GC[CT]^C
BbsI         1315  GTCTTC               GAAGAC(2/6)
BcgI           95  GCAACCTTATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          929  TGATCA               T^GATCA
BcnI          890  CCGGG                CC^[GC]GG
BfrI         1255  CTTAAG               C^TTAAG
BmyI         1106  GAGCTC               G[AGT]GC[ACT]^C
BpuAI        1315  GTCTTC               GAAGAC(2/6)
BsaAI        1329  CACGTA               [CT]AC^GT[GA]
BsaBI         942  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         824  GACGTC               G[GA]^CG[CT]C
BsaJI        1058  CCAAGG               C^C[ACGT][ACGT]GG
BsaWI         191  TCCGGA               [AT]^CCGG[AT]
BseAI         191  TCCGGA               T^CCGGA
BsiHKAI      1106  GAGCTC               G[AT]GC[AT]^C
Bsp1286I     1106  GAGCTC               G[AGT]GC[ACT]^C
BspEI         191  TCCGGA               T^CCGGA
BspLU11I      336  ACATGT               A^CATGT
BstYI        1062  GGATCT               [GA]^GATC[CT]
Bsu36I        871  CCTGAGG              CC^T[ACGT]AGG
Cac8I         831  GCTAGC               GC[ACGT]^[ACGT]GC
CfoI         1338  GCGC                 GCG^C
DraII        1346  AGGGCCT              [GA]G^G[ACGT]CC[CT]
Eco47III     1337  AGCGCT               AGC^GCT
Eco81I        871  CCTGAGG              CC^T[ACGT]AGG
EcoO109I     1346  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         786  GAATTC               G^AATTC
EcoT14I      1058  CCAAGG               C^C[AT][AT]GG
FbaI          929  TGATCA               T^GATCA
HaeII        1337  AGCGCT               [GA]GCGC^[CT]
HhaI         1338  GCGC                 GCG^C
Hin1I         824  GACGTC               G[GA]^CG[CT]C
HinP1I       1338  GCGC                 G^CGC
HincII       1153  GTTGAC               GT[CT]^[GA]AC
HindII       1153  GTTGAC               GT[CT]^[GA]AC
MamI          942  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         1062  GGATCT               [GA]^GATC[CT]
MroI          191  TCCGGA               T^CCGGA
NciI          890  CCGGG                CC^[GC]GG
NheI          831  GCTAGC               G^CTAGC
PflFI        1309  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI         688  GACTTTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         1106  GAGCTC               GAGCT^C
SfaNI         942  GATGC                GCATC(5/9)
SfcI          459  CTATAG               C^T[GA][CT]AG
StuI          165  AGGCCT               AGG^CCT
StyI         1058  CCAAGG               C^C[AT][AT]GG
TaqI         1229  TCGA                 T^CGA
Tth111I      1309  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      1229  TCGA                 T^CGA
XcmI           68  CCAAAGTGACACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1062  GGATCT               [GA]^GATC[CT]
XmnI          450  GAAAATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AflIII        336  ACATGT               A^C[GA][CT]GT
             1328  ACACGT              
BalI          393  TGGCCA               TGG^CCA
             1268  TGGCCA              
BbvI         1387  GCAGC                GCAGC(8/12)
             1183  GCTGC               
BfaI          421  CTAG                 C^TAG
              832  CTAG                
BsaI          973  GAGACC               GGTCTC(1/5)
             1176  GAGACC              
BsmFI         333  GGGAC                GGGAC(10/14)
              515  GGGAC               
BspMI        1179  ACCTGC               ACCTGC(4/8)
              387  GCAGGT              
Cfr13I        669  GGGCC                G^G[ACGT]CC
             1347  GGGCC               
EaeI          393  TGGCCA               [CT]^GGCC[GA]
             1268  TGGCCA              
EarI          237  GAAGAG               CTCTTC(1/4)
              556  GAAGAG              
Fnu4HI       1183  GCTGC                GC^[ACGT]GC
             1387  GCAGC               
HapII         192  CCGG                 C^CGG
              890  CCGG                
HpaII         192  CCGG                 C^CGG
              890  CCGG                
ItaI         1183  GCTGC                GC^[ACGT]GC
             1387  GCAGC               
Ksp632I       237  GAAGAG               CTCTTC(1/4)
              556  GAAGAG              
MaeI          421  CTAG                 C^TAG
              832  CTAG                
MaeII         825  ACGT                 A^CGT
             1330  ACGT                
MluNI         393  TGGCCA               TGG^CCA
             1268  TGGCCA              
MscI          393  TGGCCA               TGG^CCA
             1268  TGGCCA              
MseI          274  TTAA                 T^TAA
             1256  TTAA                
MspI          192  CCGG                 C^CGG
              890  CCGG                
MwoI         1340  GCTTTGAGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1357  GCAAGGGGGGC         
NspI          204  ACATGC               [GA]CATG^[CT]
              336  ACATGT              
Sau96I        669  GGGCC                G^G[ACGT]CC
             1347  GGGCC               
SmlI          980  CTCAAG               C^T[CT][GA]AG
             1255  CTTAAG              
Tru9I         274  TTAA                 T^TAA
             1256  TTAA                
TseI         1183  GCTGC                G^C[AT]GC
             1387  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BpmI        
BseRI       BsiYI       BslI        BsmAI       BsrDI       
BsrI        BssKI       BstF5I      BstNI       DdeI        
DpnII       EcoRII      FokI        HaeIII      HinfI       
HphI        MaeIII      MboI        MboII       MnlI        
MslI        MvaI        NdeII       NlaIII      PleI        
RsaI        Sau3AI      ScrFI       TfiI        Tsp45I      
Tsp509I     TspRI       
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