Restriction map for pF1KSDB1316
##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

AccIII      AclI        AgeI        AhdI        Aor51HI     
ApaI        AscI        AseI        AsnI        AspEI       
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BbvCI       BclI        BglII       BlnI        
BseAI       BsiEI       BsiWI       BspDI       BspEI       
BspLU11I    BsrDI       BssHII      BssSI       Bst1107I    
BstBI       BstYI       BstZ17I     ClaI        CpoI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KasI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MunI        
NarI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SalI        SbfI        
ScaI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        XbaI        XhoII       


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

Acc65I       1083  GGTACC               G^GTACC
AccI          691  GTCTAC               GT^[AC][GT]AC
AflII        2182  CTTAAG               C^TTAAG
AflIII        371  ACGTGT               A^C[GA][CT]GT
AocI          789  CCTGAGG              CC^T[ACGT]AGG
Asp700I       569  GAAATTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1083  GGTACC               G^GTACC
AspI          685  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BciVI        2272  GGATAC               GTATCC(6/5)
BfaI          626  CTAG                 C^TAG
BfrI         2182  CTTAAG               C^TTAAG
BlpI          162  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      162  GCTGAGC              GC^T[ACGT]AGC
BsaAI         370  TACGTG               [CT]AC^GT[GA]
BsaBI        2133  GATCGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1277  ACCGGA               [AT]^CCGG[AT]
BsmBI        2371  GAGACG               CGTCTC(1/5)
BsmI          388  GCATTC               GAATGC(1/-1)
Bsp1407I      185  TGTACA               T^GTACA
BspHI         126  TCATGA               T^CATGA
BsrGI         185  TGTACA               T^GTACA
BstEII       1209  GGTAACC              G^GT[ACGT]ACC
BstPI        1209  GGTAACC              G^GT[ACGT]ACC
Bsu36I        789  CCTGAGG              CC^T[ACGT]AGG
CelII         162  GCTGAGC              GC^T[ACGT]AGC
EaeI          847  CGGCCA               [CT]^GGCC[GA]
Eco81I        789  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1872  CCTGTCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1209  GGTAACC              G^GT[ACGT]ACC
HaeII         355  AGCGCC               [GA]GCGC^[CT]
KpnI         1083  GGTACC               GGTAC^C
KspI         1093  CCGCGG               CCGC^GG
MaeI          626  CTAG                 C^TAG
MamI         2133  GATCGTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI          396  GCCGGC               GCC^GGC
NgoMIV        396  GCCGGC               G^CCGGC
NspI         1025  ACATGC               [GA]CATG^[CT]
PaeR7I       1360  CTCGAG               C^TCGAG
PflFI         685  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuII         946  CAGCTG               CAG^CTG
RcaI          126  TCATGA               T^CATGA
SacI         1337  GAGCTC               GAGCT^C
SacII        1093  CCGCGG               CCGC^GG
SapI         1395  GCTCTTC              GCTCTTC(1/4)
SexAI         113  ACCTGGT              A^CC[AT]GGT
SfiI         1491  GGCCCCGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI         185  TGTACA               T^GTACA
TfiI         1626  GATTC                G^A[AT]TC
Tth111I       685  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         1360  CTCGAG               C^TCGAG
XmnI          569  GAAATTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AatII         528  GACGTC               GACGT^C
             2539  GACGTC              
AccII        1094  CGCG                 CG^CG
             1544  CGCG                
AcyI          528  GACGTC               G[GA]^CG[CT]C
             2539  GACGTC              
BcgI          832  CGACTCATCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2511  CGACCTTTCTGC        
BpmI         1100  CTGGAG               CTGGAG(16/14)
             1563  CTGGAG              
BsaHI         528  GACGTC               G[GA]^CG[CT]C
             2539  GACGTC              
BsrBI        2301  CCGCTC               CCGCTC(-3/-3)
             1638  GAGCGG              
BsrI         1414  CCAGT                ACTGG(1/-1)
             1866  CCAGT               
BstUI        1094  CGCG                 CG^CG
             1544  CGCG                
BstXI        2009  CCAAAGTGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2400  CCAGCAGAATGG        
DraIII        112  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1056  CACCAAGTG           
EcoT14I       610  CCTTGG               C^C[AT][AT]GG
             2031  CCAAGG              
HgaI         1167  GACGC                GACGC(5/10)
             2572  GACGC               
Hin1I         528  GACGTC               G[GA]^CG[CT]C
             2539  GACGTC              
MvnI         1094  CGCG                 CG^CG
             1544  CGCG                
PflMI        1263  CCAGATCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1284  CCAGGCCCTGG         
PstI          343  CTGCAG               CTGCA^G
             1171  CTGCAG              
SfcI          343  CTGCAG               C^T[GA][CT]AG
             1171  CTGCAG              
SmlI         1360  CTCGAG               C^T[CT][GA]AG
             2182  CTTAAG              
StyI          610  CCTTGG               C^C[AT][AT]GG
             2031  CCAAGG              
Tsp45I        404  GTGAC                ^GT[GC]AC
             2348  GTGAC               
Van91I       1263  CCAGATCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1284  CCAGGCCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        Alw44I      
AlwI        AlwNI       ApaLI       ApoI        AspHI       
AvaI        AvaII       BanI        BanII       BbsI        
BbvI        BcnI        BglI        BmyI        BpuAI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BssKI       BstAPI      
BstF5I      BstNI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EarI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PpuMI       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SmaI        TaqI        Tru9I       
TseI        Tsp509I     TspRI       TthHB8I     XcmI        
XmaCI       XmaI        
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