Restriction map for pF1KE2504
 (Linear) (Six-base) MAPSORT of: KIEE2504.seq  Check: 8729  from: 1  to: 3042



KIEE2504.orf

 With 182 enzymes: SgfI * 

                        February 16, 2012 13:36  ..

AatII G_ACGT'C

Cuts at:      0     944    1385    3042 
   Size:        944     441    1657

  Fragments arranged by size: 

                1657     944     441
 
AccI GT'mk_AC

Cuts at:      0    2900    3042 
   Size:       2900     142
 
Acc65I G'GTAC_C

Cuts at:      0     152     470    3042 
   Size:        152     318    2572

  Fragments arranged by size: 

                2572     318     152
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     534     604     802    1136    1390    1641    2062    2444 
   Size:        534      70     198     334     254     251     421     382

Cuts at:   2444    2666    2815    2858    3016    3042 
   Size:        222     149      43     158      26

  Fragments arranged by size: 

                 534     421     382     334     254     251     222     198
                 158     149      70      43      26
 
AflIII A'CryG_T

Cuts at:      0    2666    2783    3042 
   Size:       2666     117     259

  Fragments arranged by size: 

                2666     259     117
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1465    1501    1722    1763    2187    3042 
   Size:       1465      36     221      41     424     855

  Fragments arranged by size: 

                1465     855     424     221      41      36
 
ApoI r'AATT_y

Cuts at:      0    1012    1887    3016    3042 
   Size:       1012     875    1129      26

  Fragments arranged by size: 

                1129    1012     875      26
 
AscI GG'CGCG_CC

Cuts at:      0      51    3042 
   Size:         51    2991
 
AvaI C'yCGr_G

Cuts at:      0     520     894    1192    1243    3042 
   Size:        520     374     298      51    1799

  Fragments arranged by size: 

                1799     520     374     298      51
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     136     169    3042 
   Size:        136      33    2873

  Fragments arranged by size: 

                2873     136      33
 
BamHI G'GATC_C

Cuts at:      0    1599    2361    2612    3042 
   Size:       1599     762     251     430

  Fragments arranged by size: 

                1599     762     430     251
 
BanI G'GyrC_C

Cuts at:      0     152     194     470     868    1978    3042 
   Size:        152      42     276     398    1110    1064

  Fragments arranged by size: 

                1110    1064     398     276     152      42
 
BbsI GAAGACnn'nnnn_

Cuts at:      0       2     265     328     900    1647    2620    3042 
   Size:          2     263      63     572     747     973     422

  Fragments arranged by size: 

                 973     747     572     422     263      63       2
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     511     545     846     880    3042 
   Size:        511      34     301      34    2162

  Fragments arranged by size: 

                2162     511     301      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1910    3042 
   Size:       1910    1132
 
BglI GCCn_nnn'nGGC

Cuts at:      0     715    1093    1273    3042 
   Size:        715     378     180    1769

  Fragments arranged by size: 

                1769     715     378     180
 
BlpI GC'TnA_GC

Cuts at:      0    2101    3042 
   Size:       2101     941
 
Bme1580I G_kGCm'C

Cuts at:      0     873    3042 
   Size:        873    2169
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     944    3042 
   Size:        944    2098
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     909    3042 
   Size:        909    2133
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293    1360    1657    1744    1942    3042 
   Size:        293    1067     297      87     198    1100

  Fragments arranged by size: 

                1100    1067     297     293     198      87
 
Bpu10I CC'TnA_GC

Cuts at:      0    2332    2984    3042 
   Size:       2332     652      58

  Fragments arranged by size: 

                2332     652      58
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     526    1018    3042 
   Size:        526     492    2024

  Fragments arranged by size: 

                2024     526     492
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2706    2732    3042 
   Size:       2706      26     310

  Fragments arranged by size: 

                2706     310      26
 
BsaAI yAC'GTr

Cuts at:      0     371    3042 
   Size:        371    2671
 
BsaBI GATnn'nnATC

Cuts at:      0     106    3042 
   Size:        106    2936
 
BsaHI Gr'CG_yC

Cuts at:      0     941    1382    3042 
   Size:        941     441    1660

  Fragments arranged by size: 

                1660     941     441
 
BsaWI w'CCGG_w

Cuts at:      0      98     986    3042 
   Size:         98     888    2056

  Fragments arranged by size: 

                2056     888      98
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     145     175    1580    1610    2686    2716    3042 
   Size:        145      30    1405      30    1076      30     326

  Fragments arranged by size: 

                1405    1076     326     145      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     290     517    1134    1601    3042 
   Size:        290     227     617     467    1441

  Fragments arranged by size: 

                1441     617     467     290     227
 
BseYI C'CCAG_C

Cuts at:      0     507    2608    3042 
   Size:        507    2101     434

  Fragments arranged by size: 

                2101     507     434
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     915    2293    3042 
   Size:        136     779    1378     749

  Fragments arranged by size: 

                1378     779     749     136
 
BsiHKAI G_wGCw'C

Cuts at:      0    1838    3042 
   Size:       1838    1204
 
BsmI GAATG_Cn'

Cuts at:      0     895    2536    3042 
   Size:        895    1641     506

  Fragments arranged by size: 

                1641     895     506
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1389    2735    3042 
   Size:       1389    1346     307

  Fragments arranged by size: 

                1389    1346     307
 
Bsp1286I G_dGCh'C

Cuts at:      0     873    1838    3042 
   Size:        873     965    1204

  Fragments arranged by size: 

                1204     965     873
 
BspEI T'CCGG_A

Cuts at:      0      98    3042 
   Size:         98    2944
 
BspHI T'CATG_A

Cuts at:      0    1841    3042 
   Size:       1841    1201
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2309    3042 
   Size:       2309     733
 
BsrBI CCG'CTC

Cuts at:      0      47    2250    3042 
   Size:         47    2203     792

  Fragments arranged by size: 

                2203     792      47
 
BssHII G'CGCG_C

Cuts at:      0      51    3042 
   Size:         51    2991
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      68     349     813    3042 
   Size:         68     281     464    2229

  Fragments arranged by size: 

                2229     464     281      68
 
BstEII G'GTnAC_C

Cuts at:      0     999    3042 
   Size:        999    2043
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     963    2230    3042 
   Size:        963    1267     812

  Fragments arranged by size: 

                1267     963     812
 
BstYI r'GATC_y

Cuts at:      0    1181    1599    2114    2183    2190    2361    2612    2681 
   Size:       1181     418     515      69       7     171     251      69

Cuts at:   2681    3042 
   Size:        361

  Fragments arranged by size: 

                1181     515     418     361     251     171      69      69
                   7
 
BtgI C'CryG_G

Cuts at:      0    1091    2054    3042 
   Size:       1091     963     988

  Fragments arranged by size: 

                1091     988     963
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     644     932    3042 
   Size:        644     288    2110

  Fragments arranged by size: 

                2110     644     288
 
BtsI GCAGTG_nn'

Cuts at:      0     944    1958    3042 
   Size:        944    1014    1084

  Fragments arranged by size: 

                1084    1014     944
 
ClaI AT'CG_AT

Cuts at:      0    2369    2501    3042 
   Size:       2369     132     541

  Fragments arranged by size: 

                2369     541     132
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2586    2621    3042 
   Size:       2586      35     421

  Fragments arranged by size: 

                2586     421      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2494    3042 
   Size:       2494     548
 
EaeI y'GGCC_r

Cuts at:      0     104    1097    1265    1935    3042 
   Size:        104     993     168     670    1107

  Fragments arranged by size: 

                1107     993     670     168     104
 
EarI CTCTTCn'nnn_

Cuts at:      0    1940    3042 
   Size:       1940    1102
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19    1097    1377    3042 
   Size:         19    1078     280    1665

  Fragments arranged by size: 

                1665    1078     280      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     534     604     802    1136    1360    1390    1641 
   Size:        293     241      70     198     334     224      30     251

Cuts at:   1641    1657    1744    1942    2062    2444    2666    2815    2858 
   Size:         16      87     198     120     382     222     149      43

Cuts at:   2858    3016    3042 
   Size:        158      26

  Fragments arranged by size: 

                 382     334     293     251     241     224     222     198
                 198     158     149     120      87      70      43      30
                  26      16
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2140    2620    3042 
   Size:       2140     480     422

  Fragments arranged by size: 

                2140     480     422
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1760    2010    3042 
   Size:       1760     250    1032

  Fragments arranged by size: 

                1760    1032     250
 
EcoRI G'AATT_C

Cuts at:      0    1012    3042 
   Size:       1012    2030
 
EcoRV GAT'ATC

Cuts at:      0    2505    3042 
   Size:       2505     537
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2043    2106    2791    3042 
   Size:       2043      63     685     251

  Fragments arranged by size: 

                2043     685     251      63
 
FspI TGC'GCA

Cuts at:      0    1628    3042 
   Size:       1628    1414
 
HaeII r_GCGC'y

Cuts at:      0    1088    2653    3042 
   Size:       1088    1565     389

  Fragments arranged by size: 

                1565    1088     389
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     205     237    1173    1205    1956    1988    2175    2207 
   Size:        205      32     936      32     751      32     187      32

Cuts at:   2207    2686    2718    3042 
   Size:        479      32     324

  Fragments arranged by size: 

                 936     751     479     324     205     187      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    1256    2376    2412    3042 
   Size:       1256    1120      36     630

  Fragments arranged by size: 

                1256    1120     630      36
 
HindIII A'AGCT_T

Cuts at:      0     697    3042 
   Size:        697    2345
 
KpnI G_GTAC'C

Cuts at:      0     156     474    3042 
   Size:        156     318    2568

  Fragments arranged by size: 

                2568     318     156
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     383    1196    3042 
   Size:        383     813    1846

  Fragments arranged by size: 

                1846     813     383
 
MscI TGG'CCA

Cuts at:      0     106    1937    3042 
   Size:        106    1831    1105

  Fragments arranged by size: 

                1831    1105     106
 
MslI CAynn'nnrTG

Cuts at:      0     112    2020    2314    2404    3042 
   Size:        112    1908     294      90     638

  Fragments arranged by size: 

                1908     638     294     112      90
 
MspA1I CmG'CkG

Cuts at:      0     265     467     903    1594    3042 
   Size:        265     202     436     691    1448

  Fragments arranged by size: 

                1448     691     436     265     202
 
NcoI C'CATG_G

Cuts at:      0    2054    3042 
   Size:       2054     988
 
NspI r_CATG'y

Cuts at:      0    1633    2670    2787    3042 
   Size:       1633    1037     117     255

  Fragments arranged by size: 

                1633    1037     255     117
 
PasI CC'CwG_GG

Cuts at:      0    2622    3042 
   Size:       2622     420
 
PciI A'CATG_T

Cuts at:      0    2666    2783    3042 
   Size:       2666     117     259

  Fragments arranged by size: 

                2666     259     117
 
PfoI T'CCnGG_A

Cuts at:      0    1204    1376    1634    2186    2590    3042 
   Size:       1204     172     258     552     404     452

  Fragments arranged by size: 

                1204     552     452     404     258     172
 
PpuMI rG'GwC_Cy

Cuts at:      0    1760    2010    3042 
   Size:       1760     250    1032

  Fragments arranged by size: 

                1760    1032     250
 
PstI C_TGCA'G

Cuts at:      0    1718    3042 
   Size:       1718    1324
 
PvuII CAG'CTG

Cuts at:      0     903    1594    3042 
   Size:        903     691    1448

  Fragments arranged by size: 

                1448     903     691
 
SfcI C'TryA_G

Cuts at:      0     563    1714    2322    3042 
   Size:        563    1151     608     720

  Fragments arranged by size: 

                1151     720     608     563
 
SmlI C'TyrA_G

Cuts at:      0     541    1033    3042 
   Size:        541     492    2009

  Fragments arranged by size: 

                2009     541     492
 
StyI C'CwwG_G

Cuts at:      0      86    1438    1668    1817    2054    2090    2279    2775 
   Size:         86    1352     230     149     237      36     189     496

Cuts at:   2775    2825    3042 
   Size:         50     217

  Fragments arranged by size: 

                1352     496     237     230     217     189     149      86
                  50      36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     319     857     996    3042 
   Size:        319     538     139    2046

  Fragments arranged by size: 

                2046     538     319     139
 
TatI w'GTAC_w

Cuts at:      0     524    2963    3042 
   Size:        524    2439      79

  Fragments arranged by size: 

                2439     524      79
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1417    1449    1580    1612    1884    1916    3042 
   Size:       1417      32     131      32     272      32    1126

  Fragments arranged by size: 

                1417    1126     272     131      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2698    3042 
   Size:       2698     344
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     301    1089    1302    1671    3042 
   Size:        301     788     213     369    1371

  Fragments arranged by size: 

                1371     788     369     301     213
 
XmnI GAAnn'nnTTC

Cuts at:      0    1654    3042 
   Size:       1654    1388
 
ZraI GAC'GTC

Cuts at:      0     942    1383    3042 
   Size:        942     441    1659

  Fragments arranged by size: 

                1659     942     441
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI   AflIII    AlwNI     ApoI     AscI
     AvaI     BaeI    BamHI     BanI     BbsI     BcgI    BciVI     BglI
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
   BssHII   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DrdI     EaeI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     KpnI     MmeI     MscI     MslI   MspA1I     NcoI     NspI
     PasI     PciI     PfoI    PpuMI     PstI    PvuII     SfcI     SmlI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AclI     AfeI    AflII     AgeI     AhdI     AleI     AlfI
     AloI     ApaI    ApaLI     AseI    AsiSI    AvrII    BanII     BbeI
    BbvCI     BclI    BfrBI    BglII    BmgBI     BmtI    BsiEI    BsiWI
    BsrDI    BsrFI    BsrGI    BssSI    BstBI  BstZ17I   Bsu36I     DraI
   DraIII     EagI  EcoICRI     FseI    FspAI     HpaI     KasI     MfeI
     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     XbaI     XhoI     XmaI
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