Restriction map for pF1KSDB0238
##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

AatII       AccI        AclI        AflII       AgeI        
AhdI        Alw44I      Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp700I     AspEI       AviII       
AvrII       BbvCI       BclI        BfrI        BlnI        
BlpI        Bpu1102I    BsaBI       Bsp1407I    BspDI       
BspHI       BspLU11I    BsrGI       BssHII      Bst1107I    
BstBI       BstZ17I     CelII       ClaI        CpoI        
DraI        Eam1105I    Eco47III    EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HindIII     
HpaI        MamI        MfeI        MluI        MunI        
NaeI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmeI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SalI        SbfI        
SfiI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        XbaI        
XhoI        XmnI        


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# ONE CUT ENZYMES (   40 enzymes) #
###################################

Acc65I       2963  GGTACC               G^GTACC
AccIII        500  TCCGGA               T^CCGGA
AcsI         1951  GAATTT               [GA]^AATT[CT]
ApoI         1951  GAATTT               [GA]^AATT[CT]
Asp718I      2963  GGTACC               G^GTACC
AspI          856  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1109  GGATCC               G^GATCC
BciVI        2539  GGATAC               GTATCC(6/5)
BfaI         3766  CTAG                 C^TAG
BglII         524  AGATCT               A^GATCT
BseAI         500  TCCGGA               T^CCGGA
BsiWI        3015  CGTACG               C^GTACG
BsmBI        3099  CGTCTC               CGTCTC(1/5)
BsmI         1448  GCATTC               GAATGC(1/-1)
BspEI         500  TCCGGA               T^CCGGA
BsrDI        3719  GCAATG               GCAATG(2/0)
BssSI        2276  CACGAG               CACGAG(-5/-1)
BstAPI         41  GCAGCCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI         2500  CGGCCG               C^GGCCG
EclXI        2500  CGGCCG               C^GGCCG
Eco52I       2500  CGGCCG               C^GGCCG
HincII       2371  GTCAAC               GT[CT]^[GA]AC
HindII       2371  GTCAAC               GT[CT]^[GA]AC
KpnI         2963  GGTACC               GGTAC^C
KspI         2535  CCGCGG               CCGC^GG
MaeI         3766  CTAG                 C^TAG
MroI          500  TCCGGA               T^CCGGA
NspI          966  GCATGC               [GA]CATG^[CT]
PflFI         856  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        2115  GACTGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         3216  GAGCTC               GAGCT^C
SacII        2535  CCGCGG               CCGC^GG
ScaI         1703  AGTACT               AGT^ACT
SexAI         752  ACCTGGT              A^CC[AT]GGT
SmaI         2089  CCCGGG               CCC^GGG
SphI          966  GCATGC               GCATG^C
Tsp509I      1952  AATT                 ^AATT
Tth111I       856  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        2089  CCCGGG               C^CCGGG
XmaI         2089  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AflIII         20  ACGTGT               A^C[GA][CT]GT
             2330  ACACGT              
AocI         2128  CCTGAGG              CC^T[ACGT]AGG
             2266  CCTCAGG             
BbrPI        1611  CACGTG               CAC^GTG
             2199  CACGTG              
BcgI         1343  GCAAGGCCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1574  GCAGCACAATCG        
BsrBI        2209  CCGCTC               CCGCTC(-3/-3)
              547  GAGCGG              
Bsu36I       2128  CCTGAGG              CC^T[ACGT]AGG
             2266  CCTCAGG             
DrdI          676  GACCTCCGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1057  GACTGCCAAGTC        
Eco81I       2128  CCTGAGG              CC^T[ACGT]AGG
             2266  CCTCAGG             
EcoNI        2511  CCTGGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2871  CCTGGATGAGG         
HgaI         1478  GACGC                GACGC(5/10)
             1878  GACGC               
PmaCI        1611  CACGTG               CAC^GTG
             2199  CACGTG              
PmlI         1611  CACGTG               CAC^GTG
             2199  CACGTG              
SapI          238  GAAGAGC              GCTCTTC(1/4)
              726  GCTCTTC             
TfiI         1100  GAATC                G^A[AT]TC
             1149  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (  122 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbeI        
BbsI        BbvI        BcnI        BglI        BmyI        
BpmI        BpuAI       BsaAI       BsaHI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrFI       BsrI        
BssKI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DraIII      DsaI        EaeI        EarI        EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NarI        NciI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
SmlI        StuI        StyI        TaqI        Tru9I       
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XcmI        XhoII       
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