Restriction map for pF1KSDB0185
 (Linear) (Six-base) MAPSORT of: KIBB0185.seq  Check: 5685  from: 1  to: 4938



KIBB0185.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1167    4787    4916    4938 
   Size:       1167    3620     129      22

  Fragments arranged by size: 

                3620    1167     129      22
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     889    1062    1086    1744    1772    1930    2584    2838 
   Size:        889     173      24     658      28     158     654     254

Cuts at:   2838    2895    3796    4938 
   Size:         57     901    1142

  Fragments arranged by size: 

                1142     901     889     658     654     254     173     158
                  57      28      24
 
AfeI AGC'GCT

Cuts at:      0    2193    4938 
   Size:       2193    2745
 
AflIII A'CryG_T

Cuts at:      0     488    3566    4938 
   Size:        488    3078    1372

  Fragments arranged by size: 

                3078    1372     488
 
AhdI GACnn_n'nnGTC

Cuts at:      0    4446    4938 
   Size:       4446     492
 
AleI CACnn'nnGTG

Cuts at:      0     914    1529    1972    4938 
   Size:        914     615     443    2966

  Fragments arranged by size: 

                2966     914     615     443
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     922     932    1990    4235    4938 
   Size:        922      10    1058    2245     703

  Fragments arranged by size: 

                2245    1058     922     703      10
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3553    3585    4874    4906    4938 
   Size:       3553      32    1289      32      32

  Fragments arranged by size: 

                3553    1289      32      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     192     711     902    1182    1770    2426    2616    2769 
   Size:        192     519     191     280     588     656     190     153

Cuts at:   2769    2980    3248    4938 
   Size:        211     268    1690

  Fragments arranged by size: 

                1690     656     588     519     280     268     211     192
                 191     190     153
 
ApaLI G'TGCA_C

Cuts at:      0    4566    4938 
   Size:       4566     372
 
ApoI r'AATT_y

Cuts at:      0    1613    4938 
   Size:       1613    3325
 
AscI GG'CGCG_CC

Cuts at:      0    2415    4938 
   Size:       2415    2523
 
AvaI C'yCGr_G

Cuts at:      0     171    1354    1902    2743    3726    4505    4938 
   Size:        171    1183     548     841     983     779     433

  Fragments arranged by size: 

                1183     983     841     779     548     433     171
 
BamHI G'GATC_C

Cuts at:      0     110    4938 
   Size:        110    4828
 
BanI G'GyrC_C

Cuts at:      0    1531    2089    2619    2891    4005    4475    4938 
   Size:       1531     558     530     272    1114     470     463

  Fragments arranged by size: 

                1531    1114     558     530     470     463     272
 
BanII G_rGCy'C

Cuts at:      0     434     896    1898    2484    2528    2675    2816    3983 
   Size:        434     462    1002     586      44     147     141    1167

Cuts at:   3983    4506    4938 
   Size:        523     432

  Fragments arranged by size: 

                1167    1002     586     523     462     434     432     147
                 141      44
 
BbeI G_GCGC'C

Cuts at:      0    4479    4938 
   Size:       4479     459
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     393    1236    1349    1546    2144    2322    4096    4881 
   Size:        393     843     113     197     598     178    1774     785

Cuts at:   4881    4938 
   Size:         57

  Fragments arranged by size: 

                1774     843     785     598     393     197     178     113
                  57
 
BbvCI CC'TCA_GC

Cuts at:      0    1268    2692    2801    4795    4938 
   Size:       1268    1424     109    1994     143

  Fragments arranged by size: 

                1994    1424    1268     143     109
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2706    2740    3316    3350    4386    4420    4938 
   Size:       2706      34     576      34    1036      34     518

  Fragments arranged by size: 

                2706    1036     576     518      34      34      34
 
BclI T'GATC_A

Cuts at:      0     305    4938 
   Size:        305    4633
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2032    2650    3079    3202    3754    4070    4938 
   Size:       2032     618     429     123     552     316     868

  Fragments arranged by size: 

                2032     868     618     552     429     316     123
 
BglII A'GATC_T

Cuts at:      0    4871    4938 
   Size:       4871      67
 
BlpI GC'TnA_GC

Cuts at:      0     805     895    4393    4938 
   Size:        805      90    3498     545

  Fragments arranged by size: 

                3498     805     545      90
 
Bme1580I G_kGCm'C

Cuts at:      0     155     300    1660    1750    2171    2732    4010    4570 
   Size:        155     145    1360      90     421     561    1278     560

Cuts at:   4570    4938 
   Size:        368

  Fragments arranged by size: 

                1360    1278     561     560     421     368     155     145
                  90
 
BmgBI CAC'GTC

Cuts at:      0    1738    3330    4938 
   Size:       1738    1592    1608

  Fragments arranged by size: 

                1738    1608    1592
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2436    2531    3287    4938 
   Size:       2436      95     756    1651

  Fragments arranged by size: 

                2436    1651     756      95
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1917    4938 
   Size:       1917    3021
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     933    1807    1905    2203    2284    2857    2957    3738 
   Size:        933     874      98     298      81     573     100     781

Cuts at:   3738    4357    4717    4843    4938 
   Size:        619     360     126      95

  Fragments arranged by size: 

                 933     874     781     619     573     360     298     126
                 100      98      95      81
 
Bpu10I CC'TnA_GC

Cuts at:      0      10    1268    1819    2692    2801    4286    4795    4894 
   Size:         10    1258     551     873     109    1485     509      99

Cuts at:   4894    4938 
   Size:         44

  Fragments arranged by size: 

                1485    1258     873     551     509     109      99      44
                  10
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2059    4612    4938 
   Size:       2059    2553     326

  Fragments arranged by size: 

                2553    2059     326
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1124    1469    1573    2009    2254    3051    3793    4938 
   Size:       1124     345     104     436     245     797     742    1145

  Fragments arranged by size: 

                1145    1124     797     742     436     345     245     104
            
 
BsaAI yAC'GTr

Cuts at:      0    2166    3237    3567    4938 
   Size:       2166    1071     330    1371

  Fragments arranged by size: 

                2166    1371    1071     330
 
BsaBI GATnn'nnATC

Cuts at:      0     115    1417    4938 
   Size:        115    1302    3521

  Fragments arranged by size: 

                3521    1302     115
 
BsaHI Gr'CG_yC

Cuts at:      0    1913    4476    4938 
   Size:       1913    2563     462

  Fragments arranged by size: 

                2563    1913     462
 
BsaWI w'CCGG_w

Cuts at:      0    1637    3842    4938 
   Size:       1637    2205    1096

  Fragments arranged by size: 

                2205    1637    1096
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     198     228    1780    1810    1903    1933    2035    2065 
   Size:        198      30    1552      30      93      30     102      30

Cuts at:   2065    3221    3251    3262    3292    3634    3664    4015    4045 
   Size:       1156      30      11      30     342      30     351      30

Cuts at:   4045    4843    4873    4938 
   Size:        798      30      65

  Fragments arranged by size: 

                1552    1156     798     351     342     198     102      93
                  65      30      30      30      30      30      30      30
                  30      30      11
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      16      19     937    2506    2509    2655    2854    3642 
   Size:         16       3     918    1569       3     146     199     788

Cuts at:   3642    3861    4135    4564    4714    4938 
   Size:        219     274     429     150     224

  Fragments arranged by size: 

                1569     918     788     429     274     224     219     199
                 150     146      16       3       3
 
BseYI C'CCAG_C

Cuts at:      0    1182    1518    1718    2010    2147    2194    2283    2753 
   Size:       1182     336     200     292     137      47      89     470

Cuts at:   2753    2757    3059    3392    3525    3937    4068    4619    4799 
   Size:          4     302     333     133     412     131     551     180

Cuts at:   4799    4938 
   Size:        139

  Fragments arranged by size: 

                1182     551     470     412     336     333     302     292
                 200     180     139     137     133     131      89      47
                   4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     661     868     892    1756    2518    3220    3235    3630 
   Size:        661     207      24     864     762     702      15     395

Cuts at:   3630    4063    4609    4690    4938 
   Size:        433     546      81     248

  Fragments arranged by size: 

                 864     762     702     661     546     433     395     248
                 207      81      24      15
 
BsiEI CG_ry'CG

Cuts at:      0    1469    2936    3738    4938 
   Size:       1469    1467     802    1200

  Fragments arranged by size: 

                1469    1467    1200     802
 
BsiHKAI G_wGCw'C

Cuts at:      0      11     410    1040    1211    1703    2233    2675    2828 
   Size:         11     399     630     171     492     530     442     153

Cuts at:   2828    3983    4399    4570    4757    4938 
   Size:       1155     416     171     187     181

  Fragments arranged by size: 

                1155     630     530     492     442     416     399     187
                 181     171     171     153      11
 
BsmI GAATG_Cn'

Cuts at:      0    3151    4938 
   Size:       3151    1787
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3625    4938 
   Size:       3625    1313
 
Bsp1286I G_dGCh'C

Cuts at:      0      11     155     300     410     434     896    1040    1211 
   Size:         11     144     145     110      24     462     144     171

Cuts at:   1211    1660    1703    1750    1898    2171    2233    2484    2528 
   Size:        449      43      47     148     273      62     251      44

Cuts at:   2528    2675    2732    2816    2828    3983    4010    4399    4506 
   Size:        147      57      84      12    1155      27     389     107

Cuts at:   4506    4570    4757    4938 
   Size:         64     187     181

  Fragments arranged by size: 

                1155     462     449     389     273     251     187     181
                 171     148     147     145     144     144     110     107
                  84      64      62      57      47      44      43      27
                  24      12      11
 
BspEI T'CCGG_A

Cuts at:      0    1637    3842    4938 
   Size:       1637    2205    1096

  Fragments arranged by size: 

                2205    1637    1096
 
BspHI T'CATG_A

Cuts at:      0     965    4938 
   Size:        965    3973
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1167    2015    2638    3143    3815    4787    4916    4938 
   Size:       1167     848     623     505     672     972     129      22

  Fragments arranged by size: 

                1167     972     848     672     623     505     129      22
            
 
BsrBI CCG'CTC

Cuts at:      0    1823    2889    4938 
   Size:       1823    1066    2049

  Fragments arranged by size: 

                2049    1823    1066
 
BsrDI GCAATG_nn'

Cuts at:      0      94     830    2288    3122    4938 
   Size:         94     736    1458     834    1816

  Fragments arranged by size: 

                1816    1458     834     736      94
 
BsrFI r'CCGG_y

Cuts at:      0    2092    3737    4399    4938 
   Size:       2092    1645     662     539

  Fragments arranged by size: 

                2092    1645     662     539
 
BssHII G'CGCG_C

Cuts at:      0    2415    4938 
   Size:       2415    2523
 
BssSI C'ACGA_G

Cuts at:      0     766    2407    3456    4499    4938 
   Size:        766    1641    1049    1043     439

  Fragments arranged by size: 

                1641    1049    1043     766     439
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1770    3833    4005    4938 
   Size:       1770    2063     172     933

  Fragments arranged by size: 

                2063    1770     933     172
 
BstEII G'GTnAC_C

Cuts at:      0     717    1221    2830    3507    4938 
   Size:        717     504    1609     677    1431

  Fragments arranged by size: 

                1609    1431     717     677     504
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     387    2985    3558    4356    4458    4938 
   Size:        387    2598     573     798     102     480

  Fragments arranged by size: 

                2598     798     573     480     387     102
 
BstYI r'GATC_y

Cuts at:      0     110    1634    1938    4871    4938 
   Size:        110    1524     304    2933      67

  Fragments arranged by size: 

                2933    1524     304     110      67
 
Bsu36I CC'TnA_GG

Cuts at:      0    3762    3893    4938 
   Size:       3762     131    1045

  Fragments arranged by size: 

                3762    1045     131
 
BtgI C'CryG_G

Cuts at:      0     395    2318    2456    3146    3752    4019    4466    4493 
   Size:        395    1923     138     690     606     267     447      27

Cuts at:   4493    4938 
   Size:        445

  Fragments arranged by size: 

                1923     690     606     447     445     395     267     138
                  27
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     735     936    1839    1848    2007    3474    4389    4938 
   Size:        735     201     903       9     159    1467     915     549

  Fragments arranged by size: 

                1467     915     903     735     549     201     159       9
            
 
BtsI GCAGTG_nn'

Cuts at:      0    2056    2124    2535    2791    2978    3446    4938 
   Size:       2056      68     411     256     187     468    1492

  Fragments arranged by size: 

                2056    1492     468     411     256     187      68
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2430    2465    4938 
   Size:       2430      35    2473

  Fragments arranged by size: 

                2473    2430      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    2166    4938 
   Size:       2166    2772
 
EaeI y'GGCC_r

Cuts at:      0     389     635     770    1466    2033    2651    2987    3158 
   Size:        389     246     135     696     567     618     336     171

Cuts at:   3158    3239    3351    3443    3735    3755    4016    4577    4938 
   Size:         81     112      92     292      20     261     561     361

  Fragments arranged by size: 

                 696     618     567     561     389     361     336     292
                 261     246     171     135     112      92      81      20
            
 
EagI C'GGCC_G

Cuts at:      0    1466    3735    4938 
   Size:       1466    2269    1203

  Fragments arranged by size: 

                2269    1466    1203
 
EarI CTCTTCn'nnn_

Cuts at:      0      91     946     982    1636    2680    3145    3793    4938 
   Size:         91     855      36     654    1044     465     648    1145

  Fragments arranged by size: 

                1145    1044     855     654     648     465      91      36
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     206     617    3867    4938 
   Size:        206     411    3250    1071

  Fragments arranged by size: 

                3250    1071     411     206
 
EcoICRI GAG'CTC

Cuts at:      0    2673    3981    4938 
   Size:       2673    1308     957

  Fragments arranged by size: 

                2673    1308     957
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     889     933    1062    1086    1744    1772    1807    1905 
   Size:        889      44     129      24     658      28      35      98

Cuts at:   1905    1930    2203    2284    2584    2838    2857    2895    2957 
   Size:         25     273      81     300     254      19      38      62

Cuts at:   2957    3738    3796    4357    4717    4843    4938 
   Size:        781      58     561     360     126      95

  Fragments arranged by size: 

                 889     781     658     561     360     300     273     254
                 129     126      98      95      81      62      58      44
                  38      35      28      25      24      19
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     218    1432    1495    2488    2566    3456    4938 
   Size:        218    1214      63     993      78     890    1482

  Fragments arranged by size: 

                1482    1214     993     890     218      78      63
 
EcoO109I rG'GnC_Cy

Cuts at:      0      63    1398    1728    2060    2613    2747    3270    3920 
   Size:         63    1335     330     332     553     134     523     650

Cuts at:   3920    4301    4326    4560    4938 
   Size:        381      25     234     378

  Fragments arranged by size: 

                1335     650     553     523     381     378     332     330
                 234     134      63      25
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2350    4938 
   Size:       2350    2588
 
FspI TGC'GCA

Cuts at:      0     292     883    3520    4938 
   Size:        292     591    2637    1418

  Fragments arranged by size: 

                2637    1418     591     292
 
FspAI rTGC'GCAy

Cuts at:      0     883    3520    4938 
   Size:        883    2637    1418

  Fragments arranged by size: 

                2637    1418     883
 
HaeII r_GCGC'y

Cuts at:      0      25    2195    3074    3725    4293    4479    4696    4938 
   Size:         25    2170     879     651     568     186     217     242

  Fragments arranged by size: 

                2170     879     651     568     242     217     186      25
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     358     390    1903    1935    2006    2038    2471    2503 
   Size:        358      32    1513      32      71      32     433      32

Cuts at:   2503    3262    3294    4015    4047    4432    4464    4843    4875 
   Size:        759      32     721      32     385      32     379      32

Cuts at:   4875    4938 
   Size:         63

  Fragments arranged by size: 

                1513     759     721     433     385     379     358      71
                  63      32      32      32      32      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1149    2940    4938 
   Size:       1149    1791    1998

  Fragments arranged by size: 

                1998    1791    1149
 
KasI G'GCGC_C

Cuts at:      0    4475    4938 
   Size:       4475     463
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     122    2492    3074    4158    4938 
   Size:        122    2370     582    1084     780

  Fragments arranged by size: 

                2370    1084     780     582     122
 
MscI TGG'CCA

Cuts at:      0     772    2035    2653    3160    3241    4579    4938 
   Size:        772    1263     618     507      81    1338     359

  Fragments arranged by size: 

                1338    1263     772     618     507     359      81
 
MslI CAynn'nnrTG

Cuts at:      0     316     680     883     914    1529    1972    2317    3514 
   Size:        316     364     203      31     615     443     345    1197

Cuts at:   3514    3877    4456    4585    4938 
   Size:        363     579     129     353

  Fragments arranged by size: 

                1197     615     579     443     364     363     353     345
                 316     203     129      31
 
MspA1I CmG'CkG

Cuts at:      0      18     441     699     786     902    1604    1722    1950 
   Size:         18     423     258      87     116     702     118     228

Cuts at:   1950    2151    2506    2582    2757    2769    3529    4002    4194 
   Size:        201     355      76     175      12     760     473     192

Cuts at:   4194    4646    4799    4938 
   Size:        452     153     139

  Fragments arranged by size: 

                 760     702     473     452     423     355     258     228
                 201     192     175     153     139     118     116      87
                  76      18      12
 
NaeI GCC'GGC

Cuts at:      0    2094    4938 
   Size:       2094    2844
 
NarI GG'CG_CC

Cuts at:      0    4476    4938 
   Size:       4476     462
 
NcoI C'CATG_G

Cuts at:      0    2318    2456    3146    3752    4938 
   Size:       2318     138     690     606    1186

  Fragments arranged by size: 

                2318    1186     690     606     138
 
NgoMIV G'CCGG_C

Cuts at:      0    2092    4938 
   Size:       2092    2846
 
NspI r_CATG'y

Cuts at:      0     315     492     649     882    3214    4348    4938 
   Size:        315     177     157     233    2332    1134     590

  Fragments arranged by size: 

                2332    1134     590     315     233     177     157
 
PasI CC'CwG_GG

Cuts at:      0    1249    4938 
   Size:       1249    3689
 
PciI A'CATG_T

Cuts at:      0     488    4938 
   Size:        488    4450
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1626    1992    2426    3248    3594    4545    4764    4938 
   Size:       1626     366     434     822     346     951     219     174

  Fragments arranged by size: 

                1626     951     822     434     366     346     219     174
            
 
PfoI T'CCnGG_A

Cuts at:      0      77     185     620    1921    4436    4742    4938 
   Size:         77     108     435    1301    2515     306     196

  Fragments arranged by size: 

                2515    1301     435     306     196     108      77
 
PmlI CAC'GTG

Cuts at:      0    2166    3237    3567    4938 
   Size:       2166    1071     330    1371

  Fragments arranged by size: 

                2166    1371    1071     330
 
PpuMI rG'GwC_Cy

Cuts at:      0      63    1398    2060    2613    2747    3270    3920    4938 
   Size:         63    1335     662     553     134     523     650    1018

  Fragments arranged by size: 

                1335    1018     662     650     553     523     134      63
            
 
PshAI GACnn'nnGTC

Cuts at:      0    2632    4938 
   Size:       2632    2306
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     730     762    4938 
   Size:        730      32    4176

  Fragments arranged by size: 

                4176     730      32
 
PstI C_TGCA'G

Cuts at:      0      53    2051    2399    2798    3080    4913    4938 
   Size:         53    1998     348     399     282    1833      25

  Fragments arranged by size: 

                1998    1833     399     348     282      53      25
 
PvuII CAG'CTG

Cuts at:      0      18     699     786     902    1722    2151    2582    2757 
   Size:         18     681      87     116     820     429     431     175

Cuts at:   2757    2769    3529    4799    4938 
   Size:         12     760    1270     139

  Fragments arranged by size: 

                1270     820     760     681     431     429     175     139
                 116      87      18      12
 
SacI G_AGCT'C

Cuts at:      0    2675    3983    4938 
   Size:       2675    1308     955

  Fragments arranged by size: 

                2675    1308     955
 
SapI GCTCTTCn'nnn_

Cuts at:      0     982    1636    2680    3145    3793    4938 
   Size:        982     654    1044     465     648    1145

  Fragments arranged by size: 

                1145    1044     982     654     648     465
 
SbfI CC_TGCA'GG

Cuts at:      0    3080    4938 
   Size:       3080    1858
 
ScaI AGT'ACT

Cuts at:      0    1378    4938 
   Size:       1378    3560
 
SexAI A'CCwGG_T

Cuts at:      0    1225    1400    4938 
   Size:       1225     175    3538

  Fragments arranged by size: 

                3538    1225     175
 
SfcI C'TryA_G

Cuts at:      0      49    2047    2395    2794    3076    4909    4938 
   Size:         49    1998     348     399     282    1833      29

  Fragments arranged by size: 

                1998    1833     399     348     282      49      29
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2650    3202    3754    4938 
   Size:       2650     552     552    1184

  Fragments arranged by size: 

                2650    1184     552     552
 
SfoI GGC'GCC

Cuts at:      0    4477    4938 
   Size:       4477     461
 
SmaI CCC'GGG

Cuts at:      0     173    2745    3728    4938 
   Size:        173    2572     983    1210

  Fragments arranged by size: 

                2572    1210     983     173
 
SmlI C'TyrA_G

Cuts at:      0    2074    4627    4938 
   Size:       2074    2553     311

  Fragments arranged by size: 

                2553    2074     311
 
SphI G_CATG'C

Cuts at:      0     649    4938 
   Size:        649    4289
 
StuI AGG'CCT

Cuts at:      0    3322    4711    4938 
   Size:       3322    1389     227

  Fragments arranged by size: 

                3322    1389     227
 
StyI C'CwwG_G

Cuts at:      0     577    1315    1433    1874    2036    2318    2456    2702 
   Size:        577     738     118     441     162     282     138     246

Cuts at:   2702    3086    3146    3752    3986    4664    4758    4832    4938 
   Size:        384      60     606     234     678      94      74     106

  Fragments arranged by size: 

                 738     678     606     577     441     384     282     246
                 234     162     138     118     106      94      74      60
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1999    4938 
   Size:       1999    2939
 
TatI w'GTAC_w

Cuts at:      0     875    1376    4938 
   Size:        875     501    3562

  Fragments arranged by size: 

                3562     875     501
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     198     230     529     561    1780    1812    2035    2067 
   Size:        198      32     299      32    1219      32     223      32

Cuts at:   2067    3221    3253    4356    4388    4938 
   Size:       1154      32    1103      32     550

  Fragments arranged by size: 

                1219    1154    1103     550     299     223     198      32
                  32      32      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     227    1905    1989    2178    2316    3153    3501    3591 
   Size:        227    1678      84     189     138     837     348      90

Cuts at:   3591    4938 
   Size:       1347

  Fragments arranged by size: 

                1678    1347     837     348     227     189     138      90
                  84
 
XmaI C'CCGG_G

Cuts at:      0     171    2743    3726    4938 
   Size:        171    2572     983    1212

  Fragments arranged by size: 

                2572    1212     983     171
 
XmnI GAAnn'nnTTC

Cuts at:      0    3115    3427    4810    4938 
   Size:       3115     312    1383     128

  Fragments arranged by size: 

                3115    1383     312     128
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AhdI     AleI     AlfI     AloI
    AlwNI    ApaLI     ApoI     AscI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI   BstEII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
     FspI    FspAI    HaeII    Hin4I   HincII     KasI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI
     PciI    PflMI     PfoI     PmlI    PpuMI    PshAI     PsrI     PstI
    PvuII     SacI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SmaI     SmlI     SphI     StuI     StyI    TaqII     TatI
     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     ApaI     AseI
    AsiSI    AvrII     BaeI    BciVI    BfrBI     BmtI    BsiWI    BsrGI
    BstBI  BstZ17I     ClaI     DraI     DrdI    EcoRI    EcoRV     FseI
  HindIII     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PmeI     PpiI     PsiI   PspOMI    PspXI
     PvuI    RsrII    SacII     SalI    SanDI     SgfI    SgrAI    SnaBI
     SpeI     SrfI     SspI     SwaI  Tth111I     XbaI     XhoI     ZraI
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