Restriction map for pF1KSDB0068
##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

AccI        AccIII      AclI        AflII       AflIII      
AgeI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp700I     AvrII       BamHI       BbrPI       
BciVI       BclI        BfrI        BglI        BlnI        
BsaWI       BseAI       BsiEI       BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrGI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstZ17I     ClaI        CpoI        
DraI        DraIII      EagI        EclXI       Eco47III    
Eco52I      EcoO65I     EcoRI       FbaI        FseI        
HpaI        KspI        MfeI        MluI        MroI        
MunI        NheI        NotI        NruI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
SspBI       SspI        StuI        SwaI        XbaI        
XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII        2410  GACGTC               GACGT^C
Acc65I       2675  GGTACC               G^GTACC
AccII        1227  CGCG                 CG^CG
AhdI         1495  GACCAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          266  CCTCAGG              CC^T[ACGT]AGG
Asp718I      2675  GGTACC               G^GTACC
AspEI        1495  GACCAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        1220  TGCGCA               TGC^GCA
BbeI         2653  GGCGCC               GGCGC^C
BcgI         2393  GCAAGCTGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI         2224  CTAG                 C^TAG
BglII        2556  AGATCT               A^GATCT
BsaAI        2543  TACGTG               [CT]AC^GT[GA]
BsaBI         315  GATGGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BstAPI       1338  GCAGCAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1227  CGCG                 CG^CG
Bsu36I        266  CCTCAGG              CC^T[ACGT]AGG
Eam1105I     1495  GACCAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        266  CCTCAGG              CC^T[ACGT]AGG
EcoNI          54  CCTGAACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        3004  GATATC               GAT^ATC
EcoT22I       191  ATGCAT               ATGCA^T
FspI         1220  TGCGCA               TGC^GCA
HgaI          116  GCGTC                GACGC(5/10)
HincII       1008  GTTGAC               GT[CT]^[GA]AC
HindII       1008  GTTGAC               GT[CT]^[GA]AC
KasI         2653  GGCGCC               G^GCGCC
KpnI         2675  GGTACC               GGTAC^C
MaeI         2224  CTAG                 C^TAG
MamI          315  GATGGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI         1227  CGCG                 CG^CG
NaeI         1814  GCCGGC               GCC^GGC
NarI         2653  GGCGCC               GG^CGCC
NdeI          137  CATATG               CA^TATG
NgoMIV       1814  GCCGGC               G^CCGGC
NsiI          191  ATGCAT               ATGCA^T
PaeR7I       2420  CTCGAG               C^TCGAG
PshAI        2418  GACTCGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI         2874  CCTGCAGG             CCTGCA^GG
SfoI         2653  GGCGCC               GGC^GCC
SmaI         2363  CCCGGG               CCC^GGG
Sse8387I     2874  CCTGCAGG             CCTGCA^GG
XcmI         1574  CCATGCTGTCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         2420  CTCGAG               C^TCGAG
XmaCI        2363  CCCGGG               C^CCGGG
XmaI         2363  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcyI         2410  GACGTC               G[GA]^CG[CT]C
             2653  GGCGCC              
Alw44I        466  GTGCAC               G^TGCAC
             2630  GTGCAC              
ApaLI         466  GTGCAC               G^TGCAC
             2630  GTGCAC              
AspI          112  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              939  GACCAAGTC           
BsaHI        2410  GACGTC               G[GA]^CG[CT]C
             2653  GGCGCC              
BsgI          298  CTGCAC               GTGCAG(16/14)
             2435  GTGCAG              
BsmBI        1267  CGTCTC               CGTCTC(1/5)
             1072  GAGACG              
BsrBI        1920  CCGCTC               CCGCTC(-3/-3)
             1237  GAGCGG              
BsrDI         788  GCAATG               GCAATG(2/0)
             1112  GCAATG              
BssSI         469  CACGAG               CACGAG(-5/-1)
             2269  CACGAG              
DrdI          109  GACGACAGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              453  GACAAATCTGTC        
DsaI          284  CCATGG               C^C[GA][CT]GG
              365  CCATGG              
Hin1I        2410  GACGTC               G[GA]^CG[CT]C
             2653  GGCGCC              
HindIII      2230  AAGCTT               A^AGCTT
             3061  AAGCTT              
MaeII        2411  ACGT                 A^CGT
             2544  ACGT                
NcoI          284  CCATGG               C^CATGG
              365  CCATGG              
NspI          193  GCATGT               [GA]CATG^[CT]
             1937  ACATGC              
PflFI         112  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              939  GACCAAGTC           
PflMI        1506  CCAGGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1536  CCAGCTTCTGG         
PpuMI        1466  AGGTCCT              [GA]G^G[AT]CC[CT]
             2333  AGGACCT             
Tth111I       112  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              939  GACCAAGTC           
Van91I       1506  CCAGGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1536  CCAGCTTCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  104 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvCI       
BbvI        BcnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PstI        PvuII       
RsaI        SacI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
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