Restriction map for pF1KSDB0068
 (Linear) (Six-base) MAPSORT of: KIBB0068.seq  Check: 2808  from: 1  to: 3096



KIBB0068.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1424    3096 
   Size:       1424    1672
 
AatII G_ACGT'C

Cuts at:      0    2414    3096 
   Size:       2414     682
 
Acc65I G'GTAC_C

Cuts at:      0    2675    3096 
   Size:       2675     421
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2065    2218    2275    2656    3096 
   Size:       2065     153      57     381     440

  Fragments arranged by size: 

                2065     440     381     153      57
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1500    3096 
   Size:       1500    1596
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      49    2302    3096 
   Size:         49    2253     794

  Fragments arranged by size: 

                2253     794      49
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1470    1542    2295    3096 
   Size:       1470      72     753     801

  Fragments arranged by size: 

                1470     801     753      72
 
ApaLI G'TGCA_C

Cuts at:      0     466    2630    3096 
   Size:        466    2164     466

  Fragments arranged by size: 

                2164     466     466
 
ApoI r'AATT_y

Cuts at:      0     410     425     999    3096 
   Size:        410      15     574    2097

  Fragments arranged by size: 

                2097     574     410      15
 
AvaI C'yCGr_G

Cuts at:      0     338     874    1231    1376    2363    2420    2724    3096 
   Size:        338     536     357     145     987      57     304     372

  Fragments arranged by size: 

                 987     536     372     357     338     304     145      57
            
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2907    2940    3096 
   Size:       2907      33     156

  Fragments arranged by size: 

                2907     156      33
 
BanI G'GyrC_C

Cuts at:      0     238     486    1811    1964    2653    2675    2809    3096 
   Size:        238     248    1325     153     689      22     134     287

  Fragments arranged by size: 

                1325     689     287     248     238     153     134      22
            
 
BanII G_rGCy'C

Cuts at:      0    1178    1877    2192    2450    2725    2821    3096 
   Size:       1178     699     315     258     275      96     275

  Fragments arranged by size: 

                1178     699     315     275     275     258      96
 
BbeI G_GCGC'C

Cuts at:      0    2657    3096 
   Size:       2657     439
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1060    2182    2459    3096 
   Size:       1060    1122     277     637

  Fragments arranged by size: 

                1122    1060     637     277
 
BbvCI CC'TCA_GC

Cuts at:      0     308     809    1643    3047    3096 
   Size:        308     501     834    1404      49

  Fragments arranged by size: 

                1404     834     501     308      49
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2382    2416    3096 
   Size:       2382      34     680

  Fragments arranged by size: 

                2382     680      34
 
BfrBI ATG'CAT

Cuts at:      0     193    3096 
   Size:        193    2903
 
BglII A'GATC_T

Cuts at:      0    2556    3096 
   Size:       2556     540
 
BlpI GC'TnA_GC

Cuts at:      0    1016    1094    1099    1408    3096 
   Size:       1016      78       5     309    1688

  Fragments arranged by size: 

                1688    1016     309      78       5
 
Bme1580I G_kGCm'C

Cuts at:      0     470     817    2634    3096 
   Size:        470     347    1817     462

  Fragments arranged by size: 

                1817     470     462     347
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1192    3096 
   Size:       1192    1904
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     746     847    1108    1492    1606    1816    1843    1996 
   Size:        746     101     261     384     114     210      27     153

Cuts at:   1996    2116    2197    2356    2368    2644    3096 
   Size:        120      81     159      12     276     452

  Fragments arranged by size: 

                 746     452     384     276     261     210     159     153
                 120     114     101      81      27      12
 
Bpu10I CC'TnA_GC

Cuts at:      0     308     809    1439    1643    3047    3096 
   Size:        308     501     630     204    1404      49

  Fragments arranged by size: 

                1404     630     501     308     204      49
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     158     400    1318    2010    2323    2518    3096 
   Size:        158     242     918     692     313     195     578

  Fragments arranged by size: 

                 918     692     578     313     242     195     158
 
BsaI GGTCTCn'nnnn_

Cuts at:      0       1     931    1180    3096 
   Size:          1     930     249    1916

  Fragments arranged by size: 

                1916     930     249       1
 
BsaAI yAC'GTr

Cuts at:      0    2545    3096 
   Size:       2545     551
 
BsaBI GATnn'nnATC

Cuts at:      0     319    3096 
   Size:        319    2777
 
BsaHI Gr'CG_yC

Cuts at:      0    2411    2654    3096 
   Size:       2411     243     442

  Fragments arranged by size: 

                2411     442     243
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     346     376    1109    1139    1579    1609    3096 
   Size:        346      30     733      30     440      30    1487

  Fragments arranged by size: 

                1487     733     440     346      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     547    1168    1198    1249    1267    1270    1394    1489 
   Size:        547     621      30      51      18       3     124      95

Cuts at:   1489    1531    1756    1759    1882    2365    3096 
   Size:         42     225       3     123     483     731

  Fragments arranged by size: 

                 731     621     547     483     225     124     123      95
                  51      42      30      18       3       3
 
BseYI C'CCAG_C

Cuts at:      0    1147    2781    2887    3096 
   Size:       1147    1634     106     209

  Fragments arranged by size: 

                1634    1147     209     106
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283    2456    3096 
   Size:        283    2173     640

  Fragments arranged by size: 

                2173     640     283
 
BsiHKAI G_wGCw'C

Cuts at:      0      38     470    1178    1877    2192    2281    2634    2725 
   Size:         38     432     708     699     315      89     353      91

Cuts at:   2725    3096 
   Size:        371

  Fragments arranged by size: 

                 708     699     432     371     353     315      91      89
                  38
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1066    1273    3096 
   Size:       1066     207    1823

  Fragments arranged by size: 

                1823    1066     207
 
Bsp1286I G_dGCh'C

Cuts at:      0      38     470     817    1178    1877    2192    2281    2450 
   Size:         38     432     347     361     699     315      89     169

Cuts at:   2450    2634    2725    2821    3096 
   Size:        184      91      96     275

  Fragments arranged by size: 

                 699     432     361     347     315     275     184     169
                  96      91      89      38
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1424    1455    2882    3035    3096 
   Size:       1424      31    1427     153      61

  Fragments arranged by size: 

                1427    1424     153      61      31
 
BsrBI CCG'CTC

Cuts at:      0    1239    1922    3096 
   Size:       1239     683    1174

  Fragments arranged by size: 

                1239    1174     683
 
BsrDI GCAATG_nn'

Cuts at:      0     795    1119    3096 
   Size:        795     324    1977

  Fragments arranged by size: 

                1977     795     324
 
BsrFI r'CCGG_y

Cuts at:      0    1103    1814    2132    2672    3096 
   Size:       1103     711     318     540     424

  Fragments arranged by size: 

                1103     711     540     424     318
 
BssSI C'ACGA_G

Cuts at:      0     469    2269    3096 
   Size:        469    1800     827

  Fragments arranged by size: 

                1800     827     469
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1344    3096 
   Size:       1344    1752
 
BstYI r'GATC_y

Cuts at:      0    1259    1668    1916    2556    3096 
   Size:       1259     409     248     640     540

  Fragments arranged by size: 

                1259     640     540     409     248
 
Bsu36I CC'TnA_GG

Cuts at:      0     267    3096 
   Size:        267    2829
 
BtgI C'CryG_G

Cuts at:      0     284     365    3096 
   Size:        284      81    2731

  Fragments arranged by size: 

                2731     284      81
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1209    2009    3096 
   Size:       1209     800    1087

  Fragments arranged by size: 

                1209    1087     800
 
BtsI GCAGTG_nn'

Cuts at:      0     986    1027    2937    3096 
   Size:        986      41    1910     159

  Fragments arranged by size: 

                1910     986     159      41
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     115     459    3096 
   Size:        115     344    2637

  Fragments arranged by size: 

                2637     344     115
 
EaeI y'GGCC_r

Cuts at:      0    1568    2003    2772    3096 
   Size:       1568     435     769     324

  Fragments arranged by size: 

                1568     769     435     324
 
EarI CTCTTCn'nnn_

Cuts at:      0    1352    1516    1967    2251    2269    2854    3096 
   Size:       1352     164     451     284      18     585     242

  Fragments arranged by size: 

                1352     585     451     284     242     164      18
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19    1252    3096 
   Size:         19    1233    1844

  Fragments arranged by size: 

                1844    1233      19
 
EcoICRI GAG'CTC

Cuts at:      0    1176    1875    2190    2723    3096 
   Size:       1176     699     315     533     373

  Fragments arranged by size: 

                1176     699     533     373     315
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     746     847    1108    1492    1606    1816    1843    1996 
   Size:        746     101     261     384     114     210      27     153

Cuts at:   1996    2065    2116    2197    2218    2275    2356    2368    2644 
   Size:         69      51      81      21      57      81      12     276

Cuts at:   2644    2656    3096 
   Size:         12     440

  Fragments arranged by size: 

                 746     440     384     276     261     210     153     114
                 101      81      81      69      57      51      27      21
                  12      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      58    3096 
   Size:         58    3038
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1467    2334    2619    3096 
   Size:       1467     867     285     477

  Fragments arranged by size: 

                1467     867     477     285
 
EcoRV GAT'ATC

Cuts at:      0    3006    3096 
   Size:       3006      90
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     238    2508    3096 
   Size:        238    2270     588

  Fragments arranged by size: 

                2270     588     238
 
FspI TGC'GCA

Cuts at:      0    1222    3096 
   Size:       1222    1874
 
FspAI rTGC'GCAy

Cuts at:      0    1222    3096 
   Size:       1222    1874
 
HaeII r_GCGC'y

Cuts at:      0    1143    2657    2673    3096 
   Size:       1143    1514      16     423

  Fragments arranged by size: 

                1514    1143     423      16
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     928     960    1192    1224    1251    1283    1486    1518 
   Size:        928      32     232      32      27      32     203      32

Cuts at:   1518    1540    1572    1653    1685    1754    1786    1864    1896 
   Size:         22      32      81      32      69      32      78      32

Cuts at:   1896    2097    2129    3096 
   Size:        201      32     967

  Fragments arranged by size: 

                 967     928     232     203     201      81      78      69
                  32      32      32      32      32      32      32      32
                  32      27      22
 
HincII GTy'rAC

Cuts at:      0    1010    3096 
   Size:       1010    2086
 
HindIII A'AGCT_T

Cuts at:      0    2230    3061    3096 
   Size:       2230     831      35

  Fragments arranged by size: 

                2230     831      35
 
KasI G'GCGC_C

Cuts at:      0    2653    3096 
   Size:       2653     443
 
KpnI G_GTAC'C

Cuts at:      0    2679    3096 
   Size:       2679     417
 
MscI TGG'CCA

Cuts at:      0    1570    2005    2774    3096 
   Size:       1570     435     769     322

  Fragments arranged by size: 

                1570     769     435     322
 
MslI CAynn'nnrTG

Cuts at:      0      31     194     589     952    1216    3096 
   Size:         31     163     395     363     264    1880

  Fragments arranged by size: 

                1880     395     363     264     163      31
 
MspA1I CmG'CkG

Cuts at:      0     312     966    1401    1602    1821    2139    2538    3096 
   Size:        312     654     435     201     219     318     399     558

  Fragments arranged by size: 

                 654     558     435     399     318     312     219     201
            
 
NaeI GCC'GGC

Cuts at:      0    1816    3096 
   Size:       1816    1280
 
NarI GG'CG_CC

Cuts at:      0    2654    3096 
   Size:       2654     442
 
NcoI C'CATG_G

Cuts at:      0     284     365    3096 
   Size:        284      81    2731

  Fragments arranged by size: 

                2731     284      81
 
NdeI CA'TA_TG

Cuts at:      0     138    3096 
   Size:        138    2958
 
NgoMIV G'CCGG_C

Cuts at:      0    1814    3096 
   Size:       1814    1282
 
NsiI A_TGCA'T

Cuts at:      0     195    3096 
   Size:        195    2901
 
NspI r_CATG'y

Cuts at:      0     197    1941    3096 
   Size:        197    1744    1155

  Fragments arranged by size: 

                1744    1155     197
 
PasI CC'CwG_GG

Cuts at:      0    1164    3096 
   Size:       1164    1932
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1512    1542    3096 
   Size:       1512      30    1554

  Fragments arranged by size: 

                1554    1512      30
 
PfoI T'CCnGG_A

Cuts at:      0     863    1469    2150    3096 
   Size:        863     606     681     946

  Fragments arranged by size: 

                 946     863     681     606
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     976    1008    1732    1764    3096 
   Size:        976      32     724      32    1332

  Fragments arranged by size: 

                1332     976     724      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1467    2334    3096 
   Size:       1467     867     762

  Fragments arranged by size: 

                1467     867     762
 
PshAI GACnn'nnGTC

Cuts at:      0    2422    3096 
   Size:       2422     674
 
PsiI TTA'TAA

Cuts at:      0     926    3096 
   Size:        926    2170
 
PspXI vC'TCGA_Gb

Cuts at:      0    2420    3096 
   Size:       2420     676
 
PstI C_TGCA'G

Cuts at:      0    1466    2105    2291    2879    3096 
   Size:       1466     639     186     588     217

  Fragments arranged by size: 

                1466     639     588     217     186
 
PvuII CAG'CTG

Cuts at:      0     312    1821    2139    2538    3096 
   Size:        312    1509     318     399     558

  Fragments arranged by size: 

                1509     558     399     318     312
 
SacI G_AGCT'C

Cuts at:      0    1178    1877    2192    2725    3096 
   Size:       1178     699     315     533     371

  Fragments arranged by size: 

                1178     699     533     371     315
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1352    1516    2251    2269    3096 
   Size:       1352     164     735      18     827

  Fragments arranged by size: 

                1352     827     735     164      18
 
SbfI CC_TGCA'GG

Cuts at:      0    2879    3096 
   Size:       2879     217
 
SfcI C'TryA_G

Cuts at:      0    1462    1606    2101    2287    2875    3096 
   Size:       1462     144     495     186     588     221

  Fragments arranged by size: 

                1462     588     495     221     186     144
 
SfoI GGC'GCC

Cuts at:      0    2655    3096 
   Size:       2655     441
 
SmaI CCC'GGG

Cuts at:      0    2365    3096 
   Size:       2365     731
 
SmlI C'TyrA_G

Cuts at:      0     173     379    1333    1989    2338    2420    2497    3096 
   Size:        173     206     954     656     349      82      77     599

  Fragments arranged by size: 

                 954     656     599     349     206     173      82      77
            
 
StyI C'CwwG_G

Cuts at:      0     102     284     365    2645    2957    3096 
   Size:        102     182      81    2280     312     139

  Fragments arranged by size: 

                2280     312     182     139     102      81
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2808    2901    3096 
   Size:       2808      93     195

  Fragments arranged by size: 

                2808     195      93
 
TatI w'GTAC_w

Cuts at:      0     505    2906    3096 
   Size:        505    2401     190

  Fragments arranged by size: 

                2401     505     190
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     346     378    1109    1141    3096 
   Size:        346      32     731      32    1955

  Fragments arranged by size: 

                1955     731     346      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     115     942    3096 
   Size:        115     827    2154

  Fragments arranged by size: 

                2154     827     115
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1581    3096 
   Size:       1581    1515
 
XhoI C'TCGA_G

Cuts at:      0    2420    3096 
   Size:       2420     676
 
XmaI C'CCGG_G

Cuts at:      0    2363    3096 
   Size:       2363     733
 
ZraI GAC'GTC

Cuts at:      0    2412    3096 
   Size:       2412     684
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI     AhdI     AlfI    AlwNI    ApaLI
     ApoI     AvaI     BaeI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BfrBI    BglII     BlpI Bme1580I     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DrdI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV
     FalI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     KasI
     KpnI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NsiI     NspI     PasI    PflMI     PfoI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PstI    PvuII     SacI     SapI     SbfI
     SfcI     SfoI     SmaI     SmlI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XhoI     XmaI     ZraI

 Enzymes that do not cut: 

     AccI     AclI     AfeI    AflII   AflIII     AgeI     AleI     AloI
     ApaI     AscI     AseI    AsiSI    AvrII    BamHI    BciVI     BclI
     BglI    BmgBI     BmrI     BmtI    BsaWI    BsiEI    BsiWI     BsmI
    BspEI    BspHI    BsrGI   BssHII    BstBI   BstEII    BstXI  BstZ17I
     ClaI    CspCI     DraI   DraIII     EagI    EcoRI     FseI     HpaI
     MfeI     MluI     MmeI     NheI     NotI     NruI     PacI     PciI
     PmeI     PmlI   PspOMI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     XbaI     XmnI
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